Análisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en Colombia

Ilustraciones

Autores:
Úsuga Restrepo, Jaime Andrés
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/85425
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/85425
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología
Dinámica molecular
Variación genética
Virus de la influenza
HA
Influenza
Diversidad genética
Dinámica molecular
Mutaciones
Influenza virus
Docking
Genetic diversity
Molecular dynamics
Mutations
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_968a0c5058fa79ff4f02f4906922b1f7
oai_identifier_str oai:repositorio.unal.edu.co:unal/85425
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Análisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en Colombia
dc.title.translated.eng.fl_str_mv Computational analysis of the hemagglutinin of pandemic lineage influenza A viruses in Colombia
title Análisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en Colombia
spellingShingle Análisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en Colombia
570 - Biología
Dinámica molecular
Variación genética
Virus de la influenza
HA
Influenza
Diversidad genética
Dinámica molecular
Mutaciones
Influenza virus
Docking
Genetic diversity
Molecular dynamics
Mutations
title_short Análisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en Colombia
title_full Análisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en Colombia
title_fullStr Análisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en Colombia
title_full_unstemmed Análisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en Colombia
title_sort Análisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en Colombia
dc.creator.fl_str_mv Úsuga Restrepo, Jaime Andrés
dc.contributor.advisor.none.fl_str_mv Hernández Ortiz, Juan Pablo
Ciuoderis Aponte, Karl Adolf
dc.contributor.author.none.fl_str_mv Úsuga Restrepo, Jaime Andrés
dc.contributor.researchgroup.spa.fl_str_mv Crs-Tid Center for Research and Surveillance of Tropical and Infectious Diseases
dc.contributor.orcid.spa.fl_str_mv 0009-0005-2830-908X
dc.subject.ddc.spa.fl_str_mv 570 - Biología
topic 570 - Biología
Dinámica molecular
Variación genética
Virus de la influenza
HA
Influenza
Diversidad genética
Dinámica molecular
Mutaciones
Influenza virus
Docking
Genetic diversity
Molecular dynamics
Mutations
dc.subject.lemb.none.fl_str_mv Dinámica molecular
Variación genética
Virus de la influenza
dc.subject.proposal.spa.fl_str_mv HA
Influenza
Diversidad genética
Dinámica molecular
Mutaciones
dc.subject.proposal.eng.fl_str_mv Influenza virus
Docking
Genetic diversity
Molecular dynamics
Mutations
description Ilustraciones
publishDate 2023
dc.date.issued.none.fl_str_mv 2023-08-01
dc.date.accessioned.none.fl_str_mv 2024-01-24T20:14:33Z
dc.date.available.none.fl_str_mv 2024-01-24T20:14:33Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/85425
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/85425
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.indexed.spa.fl_str_mv LaReferencia
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
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dc.publisher.faculty.spa.fl_str_mv Facultad de Minas
dc.publisher.place.spa.fl_str_mv Medellín, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Medellín
institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Hernández Ortiz, Juan Pablo0d123ac4980de4343bdf5d61e0de6ad3Ciuoderis Aponte, Karl Adolf35bbe164fc18adc697b811689aff95afÚsuga Restrepo, Jaime Andrés3b9edeaa8cbb0d361de74319597eb6e8Crs-Tid Center for Research and Surveillance of Tropical and Infectious Diseases0009-0005-2830-908X2024-01-24T20:14:33Z2024-01-24T20:14:33Z2023-08-01https://repositorio.unal.edu.co/handle/unal/85425Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/IlustracionesAunque se ha demostrado la amplia circulación del virus influenza (VI) A en cerdos y humanos en el mundo y en Colombia, existen pocos estudios que hayan caracterizado los aspectos biológicos de estos virus. Por tanto, éste trabajo se basó en el análisis computacional de la proteína de superficie hemaglutinina (HA) de cepas pandémicas del VI circulantes en el país, y su relación con un cambio en las propiedades biológicas de estos. Este trabajo se desarrolló en tres capítulos, en el primero se caracterizó la composición genómica de la HA, seguido de la identificación de las mutaciones en esta proteína y su posible contribución a la diseminación de estos virus en la región. Los últimos dos capítulos, correspondieron al análisis estructural de la HA y su interacción con los receptores celulares. En general, se sugiere que las mutaciones I322V, P84S, S204T, E375K entre otras, pudieron haber contribuido al fitness y en el establecimiento de estos virus en las poblaciones actuales en Colombia. Las mutaciones más relevantes (E375K y S163N) estaban relacionadas con el aumento de la virulencia y la capacidad de evadir la respuesta inmune. Adicionalmente, se observó un clúster filogenéticamente relacionado que presentó dos mutaciones únicas (D223X y Q224X) no reportadas previamente. Por otra parte, para los modelos de los consensos tridimensionales de la HA, se observó que el modelo cBri18 (con mutaciones únicas R46G, P283A y I299V), representativo de su clúster, mostró comportamientos estructurales ligeramente distintos a los otros modelos y presentó cambios en el loop130 del sitio de unión al receptor. Adicionalmente, luego del análisis de los modelos de HA más relevantes, se encontró que estos mantienen preferencia la unión con los receptores celulares humanos (SA2,6) sobre los aviares (SA2,3). También que el modelo cCal09 tuvo mayor afinidad por SA2,6; y que el modelo cBri18 fue el única interactuó con SA2,3, hecho que puede atribuirse a los cambios evidenciados en el loop130. En conclusión, la presencia de ciertas mutaciones en la proteína HA de VI H1N1pdm de Colombia están posiblemente influenciando las propiedades biológicas de estos virus, sin embargo, otros estudios son necesarios para poder confirmar estos hallazgos. No obstante, este conocimiento generado aporta en el fortalecimiento de las acciones en la vigilancia y control de estos virus en el país y la región. (texto tomado de la fuente)Despite circulation of influenza virus (IV) in pigs and humans globally, few studies have characterized its biological features in Colombia. Therefore, in this study a computational analysis of the hemagglutinin (HA) surface protein of IV pandemic strains circulating in the country, and its relationship with a change in their biological properties was carried out. This work was developed in three chapters, in the first one, the genomic composition of HA was characterized, followed by the identification of the mutations and their possible contribution to the dissemination of these viruses in the region. The last two chapters corresponded to the structural analysis of HA and its interaction with cell receptors. In general, the mutations I322V, P84S, S204T, E375K, among others, have contributed to the fitness and establishment of these viruses in current populations in Colombia. The most relevant changes (E375K and S163N) were related to increased virulence and the ability to evade the immune response. In addition, a phylogenetically related cluster was found that exhibited two unique changes (D223X and Q224X) not previously reported. On the other hand, for the three-dimensional consensus models of the HA, it was shown that the cBri18 model (with exclusive mutations R46G, P283A and I299V), representative of its cluster, showed structural changes in the loop130 of receptor binding domain behaviors slightly different from the other models. In addition, analyzing the most relevant HA models, it was found that they preferentially bind to human cell receptors (SA2,6) over avian (SA2,3). Also, that the cCal09 model had a higher affinity for SA2,6; and that the cBri18 model was the only one that interacted with SA2,3, a fact that can be attributed to the changes observed in loop130. In conclusion, the presence of certain mutations in the HA protein of IV H1N1pdm from Colombia might be influencing the biological properties of these viruses, however, other studies are necessary to confirm these findings. However, this generated knowledge contributes to strengthening actions in the surveillance and control of these viruses in the country and the region.MaestríaMagíster en Ingeniería - Materiales y ProcesosBioinformáticaVirologíaÁrea Curricular de Materiales y Nanotecnología103 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Minas - Maestría en Ingeniería - Materiales y ProcesosFacultad de MinasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín570 - BiologíaDinámica molecularVariación genéticaVirus de la influenzaHAInfluenzaDiversidad genéticaDinámica molecularMutacionesInfluenza virusDockingGenetic diversityMolecular dynamicsMutationsAnálisis computacional de la hemaglutinina de los virus influenza A de linaje pandémico en ColombiaComputational analysis of the hemagglutinin of pandemic lineage influenza A viruses in ColombiaTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMLaReferenciaAbraham, M. 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PLoS ONE, 7(2). https://doi.org/10.1371/JOURNAL.PONE.0030946InvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85425/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1152706894.2023.pdf1152706894.2023.pdfTesis de maestría Influenzaapplication/pdf15209046https://repositorio.unal.edu.co/bitstream/unal/85425/2/1152706894.2023.pdf365237afe49ccf6ef6f56d5dd7224b1cMD52THUMBNAIL1152706894.2023.pdf.jpg1152706894.2023.pdf.jpgGenerated Thumbnailimage/jpeg4972https://repositorio.unal.edu.co/bitstream/unal/85425/3/1152706894.2023.pdf.jpg13c6b43ae1b4b236a3c71bcadbb3109dMD53unal/85425oai:repositorio.unal.edu.co:unal/854252024-01-24 23:04:16.01Repositorio Institucional Universidad Nacional de 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