Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)

Immature embryos of Juglans neotropica (Juglandaceae) with 16 and 20 weeks of development were aseptically removed from the fruits and placed in different culture media MS (Murashige and Skoog, 1962), WPM (Lloyd and McCown, 1980) y DKW (Driver and Kuniyuki, 1984) without growth regulators for 60 day...

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Autores:
Quintero García, Oscar Darío
Jaramillo Villegas, Sonia
Tipo de recurso:
Article of journal
Fecha de publicación:
2012
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/42772
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/42772
http://bdigital.unal.edu.co/32869/
Palabra clave:
6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Cedro negro
cultivo de embriones
cultivo in vitro
embriones vegetales
germinación
Juglans neotropica
medio de cultivo
nogal
rescate
Black cedar
culture media
embryo culture
germination
in vitro culture
Juglans neotropica
plant embryos
rescue
walnut.
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_94ef13d28a3582a6be376afb0383f261
oai_identifier_str oai:repositorio.unal.edu.co:unal/42772
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)
title Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)
spellingShingle Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)
6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Cedro negro
cultivo de embriones
cultivo in vitro
embriones vegetales
germinación
Juglans neotropica
medio de cultivo
nogal
rescate
Black cedar
culture media
embryo culture
germination
in vitro culture
Juglans neotropica
plant embryos
rescue
walnut.
title_short Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)
title_full Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)
title_fullStr Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)
title_full_unstemmed Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)
title_sort Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)
dc.creator.fl_str_mv Quintero García, Oscar Darío
Jaramillo Villegas, Sonia
dc.contributor.author.spa.fl_str_mv Quintero García, Oscar Darío
Jaramillo Villegas, Sonia
dc.subject.ddc.spa.fl_str_mv 6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
topic 6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Cedro negro
cultivo de embriones
cultivo in vitro
embriones vegetales
germinación
Juglans neotropica
medio de cultivo
nogal
rescate
Black cedar
culture media
embryo culture
germination
in vitro culture
Juglans neotropica
plant embryos
rescue
walnut.
dc.subject.proposal.spa.fl_str_mv Cedro negro
cultivo de embriones
cultivo in vitro
embriones vegetales
germinación
Juglans neotropica
medio de cultivo
nogal
rescate
Black cedar
culture media
embryo culture
germination
in vitro culture
Juglans neotropica
plant embryos
rescue
walnut.
description Immature embryos of Juglans neotropica (Juglandaceae) with 16 and 20 weeks of development were aseptically removed from the fruits and placed in different culture media MS (Murashige and Skoog, 1962), WPM (Lloyd and McCown, 1980) y DKW (Driver and Kuniyuki, 1984) without growth regulators for 60 days. It was observed that embryos with 16 weeks of development did not germinate in any of the three culture media. The embryos with 20 weeks of development germinated 100% in the three culture media. It was found a marked effect of the culture media on the growth of the embryos, the MS medium was significantly better than the DKW and WPM medium (P and lt; 0.05), due to the fact that in MS medium embryos had the highest height and a better proportion of stem/root to obtain complete plantlets. After this, seedlings were adapted to environmental conditions (greenhouse).
publishDate 2012
dc.date.issued.spa.fl_str_mv 2012-01-01
dc.date.accessioned.spa.fl_str_mv 2019-06-28T11:10:09Z
dc.date.available.spa.fl_str_mv 2019-06-28T11:10:09Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/32869/
identifier_str_mv ISSN: 0120-2812
url https://repositorio.unal.edu.co/handle/unal/42772
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dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/acta_agronomica/article/view/32459
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica
Acta Agronómica
dc.relation.references.spa.fl_str_mv Quintero García, Oscar Darío and Jaramillo Villegas, Sonia (2012) Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels). Revista Acta Agronómica, Universidad Nacional de Colombia Sede Palmira, 61 (1). pp. 52-60. ISSN 0120-2812
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
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institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Quintero García, Oscar Daríoe1f6be9a-dbe6-464f-b020-774298d981c7300Jaramillo Villegas, Soniacb7755b4-7268-4d4e-b76b-e16b76dc4cbc3002019-06-28T11:10:09Z2019-06-28T11:10:09Z2012-01-01ISSN: 0120-2812https://repositorio.unal.edu.co/handle/unal/42772http://bdigital.unal.edu.co/32869/Immature embryos of Juglans neotropica (Juglandaceae) with 16 and 20 weeks of development were aseptically removed from the fruits and placed in different culture media MS (Murashige and Skoog, 1962), WPM (Lloyd and McCown, 1980) y DKW (Driver and Kuniyuki, 1984) without growth regulators for 60 days. It was observed that embryos with 16 weeks of development did not germinate in any of the three culture media. The embryos with 20 weeks of development germinated 100% in the three culture media. It was found a marked effect of the culture media on the growth of the embryos, the MS medium was significantly better than the DKW and WPM medium (P and lt; 0.05), due to the fact that in MS medium embryos had the highest height and a better proportion of stem/root to obtain complete plantlets. After this, seedlings were adapted to environmental conditions (greenhouse).Embriones inmaduros de cedro negro (Juglans neotropica) (Juglandaceae) con 16 y 20 semanas de desarrollo fueron removidos asépticamente de los frutos y sembrados durante 60 días en los medios de cultivo MS (Murashige y Skoog, 1962), WPM (Lloyd y McCown, 1980) y DKW (Driver y Kuniyuki, 1984), sin reguladores de crecimiento. Se observó que embriones con 16 semanas de desarrollo no germinaron en los tres medios de cultivo; por el contrario, los embriones con 20 semanas de desarrollo presentaron una germinación de 100%. Se encontró un marcado efecto del medio en el crecimiento de los embriones, siendo el medio MS mejor (P and lt; 0.05) que los medios DKW y WPM, debido a que en él los embriones presentaron mayor altura y proporción tallo/raíz para la obtención de plántulas completas.application/mswordspaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/acta_agronomica/article/view/32459Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaQuintero García, Oscar Darío and Jaramillo Villegas, Sonia (2012) Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels). Revista Acta Agronómica, Universidad Nacional de Colombia Sede Palmira, 61 (1). pp. 52-60. ISSN 0120-28126 Tecnología (ciencias aplicadas) / Technology63 Agricultura y tecnologías relacionadas / AgricultureCedro negrocultivo de embrionescultivo in vitroembriones vegetalesgerminaciónJuglans neotropicamedio de cultivonogalrescateBlack cedarculture mediaembryo culturegerminationin vitro cultureJuglans neotropicaplant embryosrescuewalnut.Germination and rescue in vitro of immature embryos of black cedar (juglans neotropica diels)Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL32459-169880-1-PB.docxapplication/vnd.openxmlformats-officedocument.wordprocessingml.document3907413https://repositorio.unal.edu.co/bitstream/unal/42772/1/32459-169880-1-PB.docx212a35650a252cfb66b7e2deeb29f020MD5132459-120082-1-PB.pdfapplication/pdf859473https://repositorio.unal.edu.co/bitstream/unal/42772/2/32459-120082-1-PB.pdffcda10821c86f36cc92707c9380006e9MD5232459-169881-1-PB.pdfapplication/pdf537079https://repositorio.unal.edu.co/bitstream/unal/42772/3/32459-169881-1-PB.pdf17ea0491f141af75bbb13513709831ccMD5332459-120081-1-PB.docapplication/msword1344000https://repositorio.unal.edu.co/bitstream/unal/42772/4/32459-120081-1-PB.doc041093451aa8af2252ae884cc097cb4cMD54THUMBNAIL32459-120082-1-PB.pdf.jpg32459-120082-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg5040https://repositorio.unal.edu.co/bitstream/unal/42772/5/32459-120082-1-PB.pdf.jpg8a417fdb5076f35af69f442abc38211dMD5532459-169881-1-PB.pdf.jpg32459-169881-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg6931https://repositorio.unal.edu.co/bitstream/unal/42772/6/32459-169881-1-PB.pdf.jpg3ecb45a8dd2a4cfcce2c8872faeb0a2fMD56unal/42772oai:repositorio.unal.edu.co:unal/427722023-02-09 23:03:42.32Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

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