Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodilla

ilustraciones, diagramas, tablas

Autores:
Roa Bohórquez, María Alejandra
Tipo de recurso:
Fecha de publicación:
2024
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/86644
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/86644
https://repositorio.unal.edu.co/
Palabra clave:
610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
Osteoartritis
Artroplastia de Reemplazo
Osteoartritis de la Rodilla
Osteoarthritis
Arthroplasty, Replacement
Osteoarthritis, Knee
Inflamación
Sinoviocitos
Células Mesenquimales
Biomarcador
Interleuquina-17A
Interleuquina-20
Inflammation
Synoviocytes
Mesenchymal Cells
Biomarker
Interleukin-17A
Interleukin-20
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_93b4f284e3ad478e14dc78d140b7530e
oai_identifier_str oai:repositorio.unal.edu.co:unal/86644
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodilla
dc.title.translated.eng.fl_str_mv Quantification and distribution of IL 17A and IL 20 as prognostic markers in patients with early knee osteoarthritis
title Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodilla
spellingShingle Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodilla
610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
Osteoartritis
Artroplastia de Reemplazo
Osteoartritis de la Rodilla
Osteoarthritis
Arthroplasty, Replacement
Osteoarthritis, Knee
Inflamación
Sinoviocitos
Células Mesenquimales
Biomarcador
Interleuquina-17A
Interleuquina-20
Inflammation
Synoviocytes
Mesenchymal Cells
Biomarker
Interleukin-17A
Interleukin-20
title_short Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodilla
title_full Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodilla
title_fullStr Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodilla
title_full_unstemmed Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodilla
title_sort Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodilla
dc.creator.fl_str_mv Roa Bohórquez, María Alejandra
dc.contributor.advisor.none.fl_str_mv Rojas Rojas, Angela Patricia
Rondón Herrera, Federico
dc.contributor.author.none.fl_str_mv Roa Bohórquez, María Alejandra
dc.contributor.orcid.spa.fl_str_mv Roa Bohórquez, María Alejandra [0009000122169224]
dc.contributor.cvlac.spa.fl_str_mv María Alejandra Roa Bohórquez
dc.subject.ddc.spa.fl_str_mv 610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
topic 610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
Osteoartritis
Artroplastia de Reemplazo
Osteoartritis de la Rodilla
Osteoarthritis
Arthroplasty, Replacement
Osteoarthritis, Knee
Inflamación
Sinoviocitos
Células Mesenquimales
Biomarcador
Interleuquina-17A
Interleuquina-20
Inflammation
Synoviocytes
Mesenchymal Cells
Biomarker
Interleukin-17A
Interleukin-20
dc.subject.decs.spa.fl_str_mv Osteoartritis
Artroplastia de Reemplazo
Osteoartritis de la Rodilla
dc.subject.decs.eng.fl_str_mv Osteoarthritis
Arthroplasty, Replacement
Osteoarthritis, Knee
dc.subject.proposal.spa.fl_str_mv Inflamación
Sinoviocitos
Células Mesenquimales
Biomarcador
Interleuquina-17A
Interleuquina-20
dc.subject.proposal.eng.fl_str_mv Inflammation
Synoviocytes
Mesenchymal Cells
Biomarker
Interleukin-17A
Interleukin-20
description ilustraciones, diagramas, tablas
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-07-29T19:35:46Z
dc.date.available.none.fl_str_mv 2024-07-29T19:35:46Z
dc.date.issued.none.fl_str_mv 2024
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/86644
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/86644
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.publisher.faculty.spa.fl_str_mv Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rojas Rojas, Angela Patriciad6d6c58bd88fbad8491e85761431333eRondón Herrera, Federico9014453c01ed18f6d6c8ce7f0b2dba06Roa Bohórquez, María Alejandra47a25eab175a0b7120f774b65a7031b3Roa Bohórquez, María Alejandra [0009000122169224]María Alejandra Roa Bohórquez2024-07-29T19:35:46Z2024-07-29T19:35:46Z2024https://repositorio.unal.edu.co/handle/unal/86644Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, tablasLa osteoartritis (OA) es la enfermedad articular más frecuente a nivel mundial y una de las principales enfermedades degenerativas que causan discapacidad. Su origen es multifactorial, siendo más habitual en la etapa de envejecimiento y en pacientes que padecen obesidad, por lo que constituyen dos aspectos que en la actualidad y a corto plazo se mantienen en aumento. La OA se diagnostica con una base clínica apoyada de la evaluación sintomática y de herramientas de imagen. Sin embargo, a pesar de su alta prevalencia suele diagnosticarse cuando el daño en la articulación es importante y no se obtiene una respuesta terapéutica satisfactoria con el tratamiento farmacológico establecido, conllevando a requerir intervenciones quirúrgicas que en la mayoría de los casos implican reemplazo articular para mejorar la calidad de vida del paciente en términos de alivio del dolor además de mantener la independencia en la realización de sus actividades diarias. Por esta razón, surge la necesidad de proponer alternativas que permitan establecer un pronóstico de la enfermedad en etapas tempranas, facilitando el inicio de un tratamiento oportuno cuando todavía no se ha extendido el daño en toda la articulación, al igual que monitorear la respuesta al tratamiento en función del tiempo tomando como base dos características importantes de la OA que corresponden a la degeneración del cartílago y al proceso inflamatorio subyacente que promueven su cronicidad e involucra la secreción de diferentes citoquinas proinflamatorias en el microambiente sinovial dentro de las cuales están interleuquina 17A (IL-17A) e interleuquina 20 (IL-20), las cuales son componentes proinflamatorios del sistema inmune que participan en la comunicación intercelular y desempeñan funciones específicas según el sistema y proceso orgánico en el que estén implicadas. De acuerdo con lo anterior, el objetivo de este trabajo fue determinar el componente inflamatorio relacionado con los niveles de IL-17A e IL-20 mediante la estandarización de cultivos de sinoviocitos similares a fibroblastos (FLS) y de células mesenquimales (MSC) a partir de muestras de tejido sinovial provenientes de pacientes con OA temprana de rodilla. Posteriormente, se evaluó la localización y distribución de IL-17A e IL-20 por técnica de microscopía de fluorescencia y luego se llevó a cabo la cuantificación de los niveles de IL-17A e IL-20 en sobrenadantes de cultivo de FLS y MSC. En conjunto, los resultados presentados demuestran mediante técnicas de microscopía y de inmunoensayo que las células FLS y MSC son componentes que ejercen un rol importante en el proceso inflamatorio generado en la OA temprana de rodilla, pues se evidenció la producción de IL-17A e IL-20 en FLS y MSC en respuesta al estímulo inflamatorio inducido con TNF-α comparado con células control de acuerdo con las diferencias en la intensidad media de fluorescencia; la distribución de IL-17A, IL-20 y de CD-90 mediante microscopía de fluorescencia se observó a nivel citoplasmático y perinuclear evidenciando una variación en la intensidad de fluorescencia en las que contenían el anticuerpo de interés. Respecto a la cuantificación por técnica ELISA se obtuvo una concentración promedio de 0,0850 pg/mL para IL-17A; y de 0,0157 pg/mL para IL-20. A partir de los resultados con la prueba ELISA no se identificaron diferencias entre los niveles de las muestras evaluadas con o sin estímulo de TNF-α, en tanto, la intensidad de producción a nivel intracelular de IL-17A e IL-20 por técnica de microscopía de fluorescencia permitieron evidenciar la presencia de estas citoquinas en un ambiente inflamatorio, así que considerando la participación de IL-17A e IL-20 durante la etapa temprana de la OA, podrían ser marcadores de apoyo pronóstico cuyos niveles son indicativo de la inflamación de bajo grado de la articulación. Estudios posteriores que contemplen un mayor número de pacientes serían necesarios para establecer su utilidad (Texto tomado de la fuente).Osteoarthritis (OA) is the most common joint disease worldwide and one of the main degenerative diseases that cause disability. Its origin is multifactorial, being more common in the aging stage and in patients who suffer from obesity, which is why they constitute two aspects that currently and in the short term continue to increase. OA is diagnosed on a clinical basis supported by symptomatic evaluation and imaging tools. However, despite its high prevalence, it is usually diagnosed when the damage to the joint is significant and a satisfactory therapeutic response is not obtained with the established pharmacological treatment, leading to the need for surgical interventions that in most cases involve joint replacement to improve. the patient's quality of life in terms of pain relief as well as maintaining independence in carrying out daily activities. For this reason, the need arises to propose alternatives that allow establishing a prognosis of the disease in early stages, facilitating the start of timely treatment when the damage has not yet spread throughout the joint, as well as monitoring the response to treatment. as a function of time based on two important characteristics of OA that correspond to the degeneration of the cartilage and the underlying inflammatory process that promote its chronicity and involves the secretion of different proinflammatory cytokines in the synovial microenvironment within which are interleukin 17A (IL -17A) and interleukin 20 (IL-20), which are pro-inflammatory components of the immune system that participate in intercellular communication and perform specific functions depending on the system and organic process in which they are involved. In accordance with the above, the objective of this work was to determine the inflammatory component related to the levels of IL-17A and IL-20 by standardizing cultures of fibroblast-like synoviocytes (FLS) and mesenchymal cells (MSC) from of synovial tissue samples from patients with early knee OA. Subsequently, the localization and distribution of IL-17A and IL-20 was evaluated by fluorescence microscopy technique and then quantification of the levels of IL-17A and IL-20 in FLS and MSC culture supernatants was carried out. Altogether, the results presented demonstrate through microscopy and immunoassay techniques that FLS and MSC cells are components that play an important role in the inflammatory process generated in early knee OA, since the production of IL-17A and IL was evident. -20 in FLS and MSC in response to the inflammatory stimulus induced with TNF-α compared to control cells according to the differences in the mean fluorescence intensity; the distribution of IL-17A, IL-20 and CD-90 by microscopy Fluorescence was observed at the cytoplasmic and perinuclear level, evidencing a variation in the fluorescence intensity in those that contained the antibody of interest. Regarding the quantification by ELISA technique, an average concentration of 0.0850 pg/mL was obtained for IL-17A; and 0.0157 pg/mL for IL-20 from the results with the ELISA test, no differences were identified between the levels of the samples evaluated with or without TNF-α stimulation, meanwhile, the intensity of production at intracellular level of IL-17A and IL-20 by fluorescence microscopy technique allowed us to demonstrate the presence of these cytokines in an inflammatory environment, so considering the participation of IL-17A and IL-20 during the early stage of OA, they could be prognostic supportive markers whose levels are indicative of low-grade inflammation of the joint. Subsequent studies that include a larger number of patients would be necessary to establish its usefulness.MaestríaMagister en Ciencias FarmacologíaLa toma de muestras de tejido sinovial en pacientes con OA temprana de rodilla estuvo a cargo de un médico especialista en reumatología en el Hospital Universitario Nacional de Colombia y los ensayos celulares se realizaron en el Departamento de Farmacia de la Universidad Nacional de Colombia para establecer la caracterización fisiopatológica de las muestras obtenidas a partir de cada paciente correspondientes a: Estandarización de cultivos primarios de sinoviocitos (FLS) y células mesenquimales (MSC) provenientes de tejido sinovial, fibroblastos de piel, análisis por microscopía de luz, microscopía de fluorescencia y Prueba ELISA.80 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - FarmacologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::615 - Farmacología y terapéutica610 - Medicina y salud::616 - EnfermedadesOsteoartritisArtroplastia de ReemplazoOsteoartritis de la RodillaOsteoarthritisArthroplasty, ReplacementOsteoarthritis, KneeInflamaciónSinoviocitosCélulas MesenquimalesBiomarcadorInterleuquina-17AInterleuquina-20InflammationSynoviocytesMesenchymal CellsBiomarkerInterleukin-17AInterleukin-20Cuantificación y distribución de IL 17A e IL 20 como marcadores pronóstico en pacientes con osteoartritis temprana de rodillaQuantification and distribution of IL 17A and IL 20 as prognostic markers in patients with early knee osteoarthritisTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TM1. 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Immunological reviews, 233(1), 233–255. https://doi.org/10.1111/j.0105-2896.2009.00859.xEstudiantesInvestigadoresPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86644/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1057602278.2024.pdf1057602278.2024.pdfTesis de Maestría en Ciencias Farmacologíaapplication/pdf2798468https://repositorio.unal.edu.co/bitstream/unal/86644/2/1057602278.2024.pdf6ed013aeb48a94eebaff50753698f6a9MD52THUMBNAIL1057602278.2024.pdf.jpg1057602278.2024.pdf.jpgGenerated Thumbnailimage/jpeg4079https://repositorio.unal.edu.co/bitstream/unal/86644/3/1057602278.2024.pdf.jpg420eef93d493a738115ac134cbff55feMD53unal/86644oai:repositorio.unal.edu.co:unal/866442024-10-04 10:11:33.066Repositorio Institucional Universidad Nacional de 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