Efecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razas
El objetivo de este estudio fue evaluar el efecto de la estimulación ovárica con gonadotropinas sobre a) la cantidad de folículos ováricos de 3-9 mm al momento de la aspiración folicular, b) la cantidad y calidad de complejos cumulus oocitos obtenidos por sesión de aspiración folicular, c) la capaci...
- Autores:
-
Ruiz Cristancho, Silvia Andrea
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/83128
- Palabra clave:
- 630 - Agricultura y tecnologías relacionadas::636 - Producción animal
Reproducción dirigida
Artificial insemination
Inseminación Artificial
Reproduction control
Gonadotropinas
Sincronización
Producción embriones in vitro
Bovinos
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Efecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razas |
| dc.title.translated.eng.fl_str_mv |
Effect of stimulation with gonadotropins on the number of follicles and the oocyte viability obtained from donnors of different breed |
| title |
Efecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razas |
| spellingShingle |
Efecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razas 630 - Agricultura y tecnologías relacionadas::636 - Producción animal Reproducción dirigida Artificial insemination Inseminación Artificial Reproduction control Gonadotropinas Sincronización Producción embriones in vitro Bovinos |
| title_short |
Efecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razas |
| title_full |
Efecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razas |
| title_fullStr |
Efecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razas |
| title_full_unstemmed |
Efecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razas |
| title_sort |
Efecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razas |
| dc.creator.fl_str_mv |
Ruiz Cristancho, Silvia Andrea |
| dc.contributor.advisor.none.fl_str_mv |
Jimenez Escobar, Claudia Zambrano Varon, Jorge Luis |
| dc.contributor.author.none.fl_str_mv |
Ruiz Cristancho, Silvia Andrea |
| dc.contributor.researchgroup.spa.fl_str_mv |
Reproducción Animal y Salud de Hato |
| dc.contributor.orcid.spa.fl_str_mv |
https://orcid.org/0000-0001-5387-4578 |
| dc.contributor.cvlac.spa.fl_str_mv |
RUIZ CRISTANCHO, SILVIA ANDREA |
| dc.contributor.researchgate.spa.fl_str_mv |
Silvia Andrea Ruiz |
| dc.contributor.googlescholar.spa.fl_str_mv |
https://scholar.google.es/citations?user=HRELhsAAAAAJ&hl=es&oi=sra |
| dc.subject.ddc.spa.fl_str_mv |
630 - Agricultura y tecnologías relacionadas::636 - Producción animal |
| topic |
630 - Agricultura y tecnologías relacionadas::636 - Producción animal Reproducción dirigida Artificial insemination Inseminación Artificial Reproduction control Gonadotropinas Sincronización Producción embriones in vitro Bovinos |
| dc.subject.lemb.spa.fl_str_mv |
Reproducción dirigida Artificial insemination Inseminación Artificial |
| dc.subject.lemb.eng.fl_str_mv |
Reproduction control |
| dc.subject.proposal.spa.fl_str_mv |
Gonadotropinas Sincronización Producción embriones in vitro Bovinos |
| description |
El objetivo de este estudio fue evaluar el efecto de la estimulación ovárica con gonadotropinas sobre a) la cantidad de folículos ováricos de 3-9 mm al momento de la aspiración folicular, b) la cantidad y calidad de complejos cumulus oocitos obtenidos por sesión de aspiración folicular, c) la capacidad de desarrollo de los mismos medida por la tasa de clivaje y de blastocistos, así como determinar si existe efecto de la raza de la donadora en las tasas de recuperación y viabilidad de oocitos obtenidos mediante aspiración folicular. Veintitrés donadoras de razas Brahman, Jersey, Gyr, Simmental y Holstein, fueron sometidas a cuatro tratamientos: G1) control (sin tratamientos farmacológicos), G2) sincronización de la onda folicular sin estimulación con gonadotropinas G3) sincronización con estimulación con FSH y G4) sincronización con estimulación con eCG, en un modelo factorial cruzado. Un efecto positivo (p<0.05) fue encontrado en términos del número de folículos disponibles al momento de la OPU en los grupos de tratamientos G2, G3 y G4 comparados con los animales del grupo control (G1). No se encontraron diferencias estadísticamente significativas entre estos tres grupos (Sincronización, FSH o eCG). La sincronización de la onda folicular, afectó positivamente la cantidad de los complejos cumulus oocitos (COC´s), tasas de clivaje y blastocistos. Adicionalmente, se encontró un efecto positivo de la raza sobre la población de folículos presentes al momento de la OPU, tasas de recuperación, número de COC´s recuperados, tasas de maduración, de clivaje y de blastocistos. En conclusión, se encontró un efecto positivo de la sincronización de la onda folicular y de la raza sobre la calidad de los oocitos obtenidos y parámetros de producción de embriones in vitro, lo cual justificaría su utilización en programas de producción de embriones in vitro, más no se encontró en efecto benéfico del uso de gonadotropinas (FSH o eCG) antes de realizar la OPU. (Texto tomado de la fuente) |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-01-25T21:28:12Z |
| dc.date.available.none.fl_str_mv |
2023-01-25T21:28:12Z |
| dc.date.issued.none.fl_str_mv |
2023-01-24 |
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Trabajo de grado - Maestría |
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info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/acceptedVersion |
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Text |
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http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/83128 |
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Universidad Nacional de Colombia |
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Repositorio Institucional Universidad Nacional de Colombia |
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https://repositorio.unal.edu.co/ |
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https://repositorio.unal.edu.co/handle/unal/83128 https://repositorio.unal.edu.co/ |
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Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
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The Influence of morphology, follicle size and Bcl-2 and bax transcripts on the developmental competence of bovine Oocytes. Reproduction in Domestic Animals, 49(4), 576–583. https://doi.org/10.1111/rda.12325 Duffy, P., Crowe, M. A., Austin, E. J., Mihm, M., Boland, M. P., & Roche, J. F. (2004). The effect of eCG or estradiol at or after norgestomet removal on follicular dynamics, estrus and ovulation in early post-partum beef cows nursing calves. Theriogenology, 61(4), 725–734. https://doi.org/10.1016/S0093-691X(03)00255-3 Ferré, L. B., Kjelland, M. E., Taiyeb, A. M., Campos-Chillon, F., & Ross, P. J. (2020). Recent progress in bovine in vitro-derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations. In Reproduction in Domestic Animals (Vol. 55, Issue 6, pp. 659–676). Blackwell Publishing Ltd. https://doi.org/10.1111/rda.13667 Gimenes, L. U., Ferraz, M. L., Fantinato-Neto, P., Chiaratti, M. R., Mesquita, L. 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Acquisition of developmental competence and in vitro growth culture of bovine oocytes. Journal of Reproduction and Development. |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Jimenez Escobar, Claudia6426808bceaa12f877cd3379048c0530Zambrano Varon, Jorge Luisfbde601acedd3ad89bc9e1e01152bad6Ruiz Cristancho, Silvia Andrea87177c7db29d41ca96403a3b473c899fReproducción Animal y Salud de Hatohttps://orcid.org/0000-0001-5387-4578RUIZ CRISTANCHO, SILVIA ANDREASilvia Andrea Ruizhttps://scholar.google.es/citations?user=HRELhsAAAAAJ&hl=es&oi=sra2023-01-25T21:28:12Z2023-01-25T21:28:12Z2023-01-24https://repositorio.unal.edu.co/handle/unal/83128Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/El objetivo de este estudio fue evaluar el efecto de la estimulación ovárica con gonadotropinas sobre a) la cantidad de folículos ováricos de 3-9 mm al momento de la aspiración folicular, b) la cantidad y calidad de complejos cumulus oocitos obtenidos por sesión de aspiración folicular, c) la capacidad de desarrollo de los mismos medida por la tasa de clivaje y de blastocistos, así como determinar si existe efecto de la raza de la donadora en las tasas de recuperación y viabilidad de oocitos obtenidos mediante aspiración folicular. Veintitrés donadoras de razas Brahman, Jersey, Gyr, Simmental y Holstein, fueron sometidas a cuatro tratamientos: G1) control (sin tratamientos farmacológicos), G2) sincronización de la onda folicular sin estimulación con gonadotropinas G3) sincronización con estimulación con FSH y G4) sincronización con estimulación con eCG, en un modelo factorial cruzado. Un efecto positivo (p<0.05) fue encontrado en términos del número de folículos disponibles al momento de la OPU en los grupos de tratamientos G2, G3 y G4 comparados con los animales del grupo control (G1). No se encontraron diferencias estadísticamente significativas entre estos tres grupos (Sincronización, FSH o eCG). La sincronización de la onda folicular, afectó positivamente la cantidad de los complejos cumulus oocitos (COC´s), tasas de clivaje y blastocistos. Adicionalmente, se encontró un efecto positivo de la raza sobre la población de folículos presentes al momento de la OPU, tasas de recuperación, número de COC´s recuperados, tasas de maduración, de clivaje y de blastocistos. En conclusión, se encontró un efecto positivo de la sincronización de la onda folicular y de la raza sobre la calidad de los oocitos obtenidos y parámetros de producción de embriones in vitro, lo cual justificaría su utilización en programas de producción de embriones in vitro, más no se encontró en efecto benéfico del uso de gonadotropinas (FSH o eCG) antes de realizar la OPU. (Texto tomado de la fuente)The objective of this study was to evaluate the effect of ovarian stimulation with gonadotropins over the number of ovarian follicles (3-9 mm), at the time of Ovum Pick Up (OPU), the quantity and quality of oocyte cumulus complexes obtained per OPU session, their developmental capacity measured by the cleavage rate and blastocyst production; Additionally the effect of donor breed on the recovery and viability rates of oocytes obtained by follicular aspiration was also evaluated. Twenty-three donors of Brahman, Jersey, Gyr, Simmental and Holstein breeds were subjected to four treatments: G1) control (without pharmacological treatments), G2) synchronization of the follicular wave without gonadotropin stimulation, G3) synchronization and FSH stimulation and G4) synchronization and eCG stimulation, in a crossed factorial model. A positive effect was found in terms of the number of follicles available at the time of OPU in G2, G3 and G4 treatments compared to control group, with no significant differences between these three groups (synchronization, FSH or eCG). Regarding the number of COC's obtained, cleavage and blastocyst rates, a positive effect of synchronization was found, but not in the animals stimulated with FSH or eCG. Additionally, a positive effect of breed was found in terms of follicular population present at OPU, number of COC's recovered, viable COC's, COC's recovery, maturation and cleavage rate as well as blastocyst production. In conclusion, a positive effect of follicular wave synchronization, was found in cows with regard to follicular population, cleavage rate and blastocyst production, which would indicate its use in vitro embryo production programs; there was no beneficial effect on the use of gonadotropins (FSH or eCG).MaestríaMagister en Salud AnimalTeriogenología y Salud de Hatoxvii, 60 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina Veterinaria y de Zootecnia - Maestría en Salud y Producción AnimalFacultad de Medicina Veterinaria y de ZootecniaBogotá - ColombiaUniversidad Nacional de Colombia - Sede Bogotá630 - Agricultura y tecnologías relacionadas::636 - Producción animalReproducción dirigidaArtificial inseminationInseminación ArtificialReproduction controlGonadotropinasSincronizaciónProducción embriones in vitroBovinosEfecto de la estimulación con gonadotropinas sobre el número de foliculos y la viabilidad de oocitos obtenidos de donadoras diferentes razasEffect of stimulation with gonadotropins on the number of follicles and the oocyte viability obtained from donnors of different breedTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAlvarez, P., Spicer, L. 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Journal of Reproduction and Development.EFECTO DE LA ESTIMULACIÓN CON GONADOTROPINAS SOBRE EL NÚMERO DE FOLICULOS Y LA VIABILIDAD DE OOCITOS OBTENIDOS DE DONADORAS DIFERENTES RAZASSistema general de regalías, Gobernación de CundinamarcaBibliotecariosEstudiantesInvestigadoresMaestrosLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83128/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53ORIGINAL52960216.2022.pdf52960216.2022.pdfTesis de Maestría en Salud Animalapplication/pdf1473909https://repositorio.unal.edu.co/bitstream/unal/83128/4/52960216.2022.pdf1efc8526ded64169ac5028c8b59cab41MD54unal/83128oai:repositorio.unal.edu.co:unal/831282023-02-13 14:36:56.094Repositorio Institucional Universidad Nacional de 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