Efecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentes

ilustraciones, fotografías, gráficas

Autores:
Ceballos Ordoñez, Leidy Johanna
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
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oai:repositorio.unal.edu.co:unal/84343
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/84343
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología::571 - Fisiología y temas relacionados
Sistemas Neurosecretores
Glucocorticoides
Corticosterona
Neurosecretory Systems
Glucocorticoids
Corticosterone
Separación materna
Ambiente enriquecido
Corticosterona
Ratas Wistar
Sistema de recompensa
Maternal separation
Enriched environment
Corticosterone
Wistar rats
Reward system
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_91f512f6681281e04ffdae181e062d94
oai_identifier_str oai:repositorio.unal.edu.co:unal/84343
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Efecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentes
dc.title.translated.eng.fl_str_mv Effect of the enriched environment, after maternal separation, on microglial morphology and corticosterone concentration, in adolescent Wistar rats
title Efecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentes
spellingShingle Efecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentes
570 - Biología::571 - Fisiología y temas relacionados
Sistemas Neurosecretores
Glucocorticoides
Corticosterona
Neurosecretory Systems
Glucocorticoids
Corticosterone
Separación materna
Ambiente enriquecido
Corticosterona
Ratas Wistar
Sistema de recompensa
Maternal separation
Enriched environment
Corticosterone
Wistar rats
Reward system
title_short Efecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentes
title_full Efecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentes
title_fullStr Efecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentes
title_full_unstemmed Efecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentes
title_sort Efecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentes
dc.creator.fl_str_mv Ceballos Ordoñez, Leidy Johanna
dc.contributor.advisor.spa.fl_str_mv Dueñas Gómez, Zulma Janeth
dc.contributor.author.spa.fl_str_mv Ceballos Ordoñez, Leidy Johanna
dc.contributor.researchgroup.spa.fl_str_mv Neurobiología y Comportamiento
dc.subject.ddc.spa.fl_str_mv 570 - Biología::571 - Fisiología y temas relacionados
topic 570 - Biología::571 - Fisiología y temas relacionados
Sistemas Neurosecretores
Glucocorticoides
Corticosterona
Neurosecretory Systems
Glucocorticoids
Corticosterone
Separación materna
Ambiente enriquecido
Corticosterona
Ratas Wistar
Sistema de recompensa
Maternal separation
Enriched environment
Corticosterone
Wistar rats
Reward system
dc.subject.decs.spa.fl_str_mv Sistemas Neurosecretores
Glucocorticoides
Corticosterona
dc.subject.decs.eng.fl_str_mv Neurosecretory Systems
Glucocorticoids
Corticosterone
dc.subject.proposal.spa.fl_str_mv Separación materna
Ambiente enriquecido
Corticosterona
Ratas Wistar
Sistema de recompensa
dc.subject.proposal.eng.fl_str_mv Maternal separation
Enriched environment
Corticosterone
Wistar rats
Reward system
description ilustraciones, fotografías, gráficas
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-07-28T01:27:03Z
dc.date.available.none.fl_str_mv 2023-07-28T01:27:03Z
dc.date.issued.none.fl_str_mv 2023-06-29
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/84343
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/84343
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.indexed.spa.fl_str_mv Bireme
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dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
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dc.format.extent.spa.fl_str_mv 106 páginas
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv Bogotá - Medicina - Maestría en Neurociencias
dc.publisher.faculty.spa.fl_str_mv Facultad de Medicina
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
institution Universidad Nacional de Colombia
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https://repositorio.unal.edu.co/bitstream/unal/84343/2/1130619363.2023.pdf
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repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Dueñas Gómez, Zulma Janethddf6ccc29fb0eb2a962c31ef7d7bb79d600Ceballos Ordoñez, Leidy Johanna9ac000cd4fc8735fe145e5a16b7dbf98Neurobiología y Comportamiento2023-07-28T01:27:03Z2023-07-28T01:27:03Z2023-06-29https://repositorio.unal.edu.co/handle/unal/84343Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficasIntroducción: En roedores como en humanos la separación materna durante la lactancia (SMDL) incide en el neurodesarrollo al ser un factor estresor, afectando el metabolismo, comportamiento y aprendizaje. Por otro lado, la exposición a un ambiente enriquecido (AE) activa el sistema nervioso central proporcionando estímulos físicos, sociales y sensoriales que conllevarían a una reducción del estrés y adaptación de respuestas neurobiológicas. Objetivo: Analizar el efecto de 15 días de ambiente enriquecido, posterior a la separación materna durante la lactancia, sobre la cantidad y morfología de la microglía y la concentración de corticosterona, en ratas Wistar machos y hembras durante la adolescencia temprana. Metodología: Se utilizaron 78 crías de ratas Wistar divididas en dos grupos, con y sin SMDL. El día 22 fueron separadas por sexo y tratamiento, y ubicadas en un AE o estándar por 15 días. Los animales se anestesiaron y perfundieron el día 37, se tomó muestra sérica para medir corticosterona y se extrajo el tejido cerebral. Resultados: el AE posterior a la SMDL no revirtió la disminución de los niveles de corticosterona de la SMDL y AE por sí solos, pero si disminuyó el número de microglías por debajo de los números de la SMDL sola, incidiendo en la adaptación de una morfología ramificada. Conclusión: La SMDL puede afectar el sistema neuroendocrino y neuroinflamatorio, mediados por la expresión de corticosterona y glucocorticoides. Aunque la exposición del AE posterior a la SMDL disminuyó el número de microglías y proporcionó una morfología ramificada, este paradigma funcionaría como un modelo de estrés que adapta el sistema garantizando un adecuado funcionamiento a estresores futuros. (Texto tomado de la fuente).Introduction: In rodents as in humans, maternal separation during breastfeeding (MSDB) as a stressor factor affects neurodevelopment, including alteration in metabolism, behavior and learning. On the other hand, exposure to an enriched environment (EA) activates the central nervous system by providing physical, social and sensory stimuli that would lead to a reduction in stress and adaptation of neurobiological responses. Objective: To analyze the effect of 15 days of enriched environment, after maternal separation during breastfeeding, on the quantity and morphology of microglia and the concentration of corticosterone, in male and female Wistar rats during early adolescence. Methodology: 78 offspring of Wistar rats were divided into two groups, with and without SMDL, were used. On day 22 they were separated by sex and treatment and placed in an EA or standard for 15 days. The animals were anesthetized and perfused on day 37, a serum sample was taken to measure corticosterone, and brain tissue was removed. Results: the EA after the MSDB did not the decrease in corticosterone levels of the baseline MSDB and EA, but it did decrease the number of microglia below the numbers of the MSDB, affecting the adaptation of a branched morphology. Conclusion: MSDB may affects the neuroendocrine and neuroinflammatory system, mediated by the expression of corticosterone and glucocorticoids. Although exposure of the EA after MSDB decreased the number of microglia and provided a branched morphology, this paradigm would work as a stress model that adapts the system, guaranteeing proper functioning to future stressors.MaestríaMagíster en NeurocienciasEfectos neurales y comportamentales del estrés106 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina - Maestría en NeurocienciasFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::571 - Fisiología y temas relacionadosSistemas NeurosecretoresGlucocorticoidesCorticosteronaNeurosecretory SystemsGlucocorticoidsCorticosteroneSeparación maternaAmbiente enriquecidoCorticosteronaRatas WistarSistema de recompensaMaternal separationEnriched environmentCorticosteroneWistar ratsReward systemEfecto del ambiente enriquecido, posterior a la separación materna, sobre la morfología microglial y concentración de corticosterona, en ratas Wistar adolescentesEffect of the enriched environment, after maternal separation, on microglial morphology and corticosterone concentration, in adolescent Wistar ratsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBireme1. 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Neuroscience Letters, 135381.doi:10.1016/j.neulet.2020.135381InvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84343/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1130619363.2023.pdf1130619363.2023.pdfTesis de Maestría en Neurocienciasapplication/pdf6080428https://repositorio.unal.edu.co/bitstream/unal/84343/2/1130619363.2023.pdf7fc4bcd69aaf78dc90cc9ce4d7def6d2MD52unal/84343oai:repositorio.unal.edu.co:unal/843432023-07-27 20:28:38.629Repositorio Institucional Universidad Nacional de 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