Introducción al análisis estructural de proteínas y glicoproteínas

ilustraciones

Autores:: Vega Castro, Nohora Angélica
Reyes Montaño, Edgar Antonio

Tipo de recurso:: Book

Fecha de publicación:: 2020

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Introducción al análisis estructural de proteínas y glicoproteínas
title	Introducción al análisis estructural de proteínas y glicoproteínas
spellingShingle	Introducción al análisis estructural de proteínas y glicoproteínas 572 - Bioquímica Bioquímica Aminoácidos Proteínas - Análisis
title_short	Introducción al análisis estructural de proteínas y glicoproteínas
title_full	Introducción al análisis estructural de proteínas y glicoproteínas
title_fullStr	Introducción al análisis estructural de proteínas y glicoproteínas
title_full_unstemmed	Introducción al análisis estructural de proteínas y glicoproteínas
title_sort	Introducción al análisis estructural de proteínas y glicoproteínas
dc.creator.fl_str_mv	Vega Castro, Nohora Angélica Reyes Montaño, Edgar Antonio
dc.contributor.author.none.fl_str_mv	Vega Castro, Nohora Angélica Reyes Montaño, Edgar Antonio
dc.subject.ddc.spa.fl_str_mv	572 - Bioquímica
topic	572 - Bioquímica Bioquímica Aminoácidos Proteínas - Análisis
dc.subject.lemb.none.fl_str_mv	Bioquímica Aminoácidos Proteínas - Análisis
description	ilustraciones
publishDate	2020
dc.date.issued.none.fl_str_mv	2020-05
dc.date.accessioned.none.fl_str_mv	2022-03-06T05:11:21Z
dc.date.available.none.fl_str_mv	2022-03-06T05:11:21Z
dc.type.spa.fl_str_mv	Libro
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_2f33
dc.type.coarversion.spa.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv	Text
format	http://purl.org/coar/resource_type/c_2f33
status_str	publishedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/81133
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
dc.identifier.eisbn.none.fl_str_mv	9789587944006
url	https://repositorio.unal.edu.co/handle/unal/81133 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia 9789587944006
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.ispartofseries.none.fl_str_mv	Colección textos;
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spelling	Atribución-NoComercial-SinDerivadas 4.0 InternacionalUniversidad Nacional de Colombia, 2020info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Vega Castro, Nohora Angélica1bac60b0381f939db8d6c334f123ddc1Reyes Montaño, Edgar Antoniocf6d1cf5625fae2e74c37a137c09067b2022-03-06T05:11:21Z2022-03-06T05:11:21Z2020-05https://repositorio.unal.edu.co/handle/unal/81133Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/9789587944006ilustracionesLos seres vivos están conformados por gran diversidad de proteínas con diferentes características físicas, químicas, estructurales y funcionales. Para entender la función de una proteína se necesita conocer su secuencia y, mejor aún, su estructura tridimensional. Hoy en día es significativo el incremento en el número de secuencias reportadas, al igual que el aumento en la determinación del número de estructuras tridimensionales por métodos experimentales e in silico. Todo este conjunto de estudios ha sido de gran importancia en la formulación de los conceptos modernos de la bioquímica y ha permitido entender la relación que se da entre estructura y función de las proteínas, esto es una premisa fundamental que guía el quehacer bioquímico. En el presente texto brindamos una descripción general de algunos de los aspectos más relevantes sobre los componentes estructurales de proteínas y glicoproteínas, así como algunas técnicas que se han usado para estudiar cada uno de los niveles estructurales que se encuentran en ellas.Introducción -- Capítulo uno Estructura primaria de proteínas -- Requisitos para establecer la secuencia de una proteína -- Procesos de fragmentación de proteínas Métodos enzimáticos -- Mapeo peptídico -- Métodos químicos -- Fragmentación de la proteína -- Secuenciación de los péptidos -- Superposición de fragmentos -- Fuentes de error en la determinación de la estructura primaria -- Limitaciones de la determinación de la estructura primaria a partir de la secuencia de adn -- Referencias -- Capítulo dos Caracterización de proteínas por espectrometría de masas (ms)-- Ionización en modo electrospray (esi) -- Ionización/desorción láser asistida por matriz (Maldi) -- Analizador -- Otros analizadores -- Analizador de trampa iónica (it) -- Ciclotrón de resonancia de iones con transformada de Fourier (ft-icr) -- Orbitrap -- Determinación de la secuencia de proteínas por espectrometría de masas -- Fragmentación de la molécula -- Determinación de la secuencia N-terminal -- Cuantificación de proteínas en estudios de proteómica -- Utilidad de la espectrometría de masas como herramienta en el análisis de problemas relacionados con proteínas -- Identificación de nuevas variantes proteínas -- Evaluación del plegamiento de las proteínas -- Referencias -- Capítulo tres Consecuencias de la determinación de la estructura primaria de proteínas -- Proteínas que tienen la misma función y están en diferentes especies -- Proteínas que surgieron por la duplicación de un gen -- Proteínas con diferente función y localización relacionadas evolutivamente -- Referencias -- Estructura secundaria -- Capítulo cuatro -- Aspectos relevantes para la formación de estructuras secundarias -- El enlace peptídico -- Ángulos de torsión -- Estructuras secundarias -- Héliceα -- Estructuraβ -- Propensiones -- Girosβ -- Estructuras supersecundarias -- Conformación de α-hélice, estructuras β y cadenas laterales -- Estabilidad -- Determinación experimental de la estructura secundaria Dispersión óptica rotatoria (dor) -- Dicroísmo circular (dc) -- Comportamiento de macromoléculas -- Aplicaciones del dicroísmo circular (dc) -- Referencias -- Capítulo cinco Estructura terciaria -- Determinación experimental de la estructura terciaria -- Determinación de la estructura de proteínas por difracción de rayos X -- Resonancia magnética nuclear -- Microscopía crioelectrónica o criomicroscopía electrónica (Cryo-em) -- Referencias -- Capítulo seis Glicoproteínas y carbohidratos -- Diversidad estructural de los oligosacáridos -- Biosíntesis de oligosacáridos en las glicoproteínas -- Análisis estructural y funcional de glicanos -- Análisis de la glicosilación en glicoproteínas -- 1. ¿La proteína es glicosilada? -- 2. Caracterización de la glicosilación en la proteína intacta -- 3. Caracterización de los oligosacáridos -- Análisis de la estructura del oligosacárido --Métodos químicos -- Métodos enzimáticos -- Métodos enzimáticos para elucidar estructura primaria de oligosacáridos -- Estudios de glicosilación, funciones biológicas y posibles aplicaciones -- Patologías asociadas a glicoproteínas -- Referencias -- Bioinformática estructural: aplicaciones del modelamiento estructural de proteínas -- Predicción de estructura secundaria y terciaria de proteínas -- Métodos para predecir estructura secundaria de proteínas -- Predicción de estructura terciaria -- Metodología sugerida para generar modelos estructurales -- Capítulo siete modelamiento estructural de proteínas -- Aplicaciones -- Conclusiones -- Referenciasprimera edición267 páginasapplication/pdfspaColección textos;Sanger F, Thompson eOp. The amino-acid sequence in the glycyl chain of insulin. 1. 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Disponible en: https://doi.org/10.1371/journal.pbio.0050076572 - BioquímicaBioquímicaAminoácidosProteínas - AnálisisIntroducción al análisis estructural de proteínas y glicoproteínasLibroinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_2f33http://purl.org/coar/version/c_970fb48d4fbd8a85TextSede BogotáBogotá, ColombiaInvestigadoresPúblico generalORIGINALIntroducción al analisis estructural de proteinas y glicoproteinas.pdfIntroducción al analisis estructural de proteinas y glicoproteinas.pdfLibro Introducción al análisis estructural de proteínas y glicoproteínasapplication/pdf12578695https://repositorio.unal.edu.co/bitstream/unal/81133/3/Introducci%c3%b3n%20al%20analisis%20estructural%20de%20proteinas%20y%20glicoproteinas.pdf53d6e964bd9a65718937279d712a2effMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81133/2/license.txt8153f7789df02f0a4c9e079953658ab2MD52THUMBNAILIntroducción al analisis estructural de proteinas y glicoproteinas.pdf.jpgIntroducción al analisis estructural de proteinas y glicoproteinas.pdf.jpgGenerated Thumbnailimage/jpeg10079https://repositorio.unal.edu.co/bitstream/unal/81133/4/Introducci%c3%b3n%20al%20analisis%20estructural%20de%20proteinas%20y%20glicoproteinas.pdf.jpgd48ed544462b2e5be9356e67eec2bcc6MD54unal/81133oai:repositorio.unal.edu.co:unal/811332024-08-04 23:09:53.825Repositorio Institucional Universidad Nacional de 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EVESURBIFBPUiBMQSBTRUNSRVRBUsONQSBHRU5FUkFMLiAqTEEgVEVTSVMgQSBQVUJMSUNBUiBERUJFIFNFUiBMQSBWRVJTScOTTiBGSU5BTCBBUFJPQkFEQS4gCgpBbCBoYWNlciBjbGljIGVuIGVsIHNpZ3VpZW50ZSBib3TDs24sIHVzdGVkIGluZGljYSBxdWUgZXN0w6EgZGUgYWN1ZXJkbyBjb24gZXN0b3MgdMOpcm1pbm9zLiBTaSB0aWVuZSBhbGd1bmEgZHVkYSBzb2JyZSBsYSBsaWNlbmNpYSwgcG9yIGZhdm9yLCBjb250YWN0ZSBjb24gZWwgYWRtaW5pc3RyYWRvciBkZWwgc2lzdGVtYS4KClVOSVZFUlNJREFEIE5BQ0lPTkFMIERFIENPTE9NQklBIC0gw5psdGltYSBtb2RpZmljYWNpw7NuIDE5LzEwLzIwMjEK

Introducción al análisis estructural de proteínas y glicoproteínas

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