Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea

ilustraciones, gráficos

Autores:: Moreno Florez, Ana Isabel

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea
dc.title.translated.eng.fl_str_mv	Production by additive manufacturing of scaffolds with antimicrobial properties from Propolis from Tame (Arauca) for bone regeneration.
title	Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea
spellingShingle	Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea 600 - Tecnología (Ciencias aplicadas)::602 - Miscelánea 660 - Ingeniería química::666 - Cerámica y tecnologías afines 680 - Manufactura para usos específicos::686 - Imprenta y actividades relacionadas Regeneración Ósea Osteomielitis Productos con Acción Antimicrobiana Ingeniería de Tejidos Própolis Antimicrobianos Antiinfecciosos Impresión Tridimensional Impresion 3D Regeneración ósea Actividad antimicrobiana Propóleos 3D printing Bone regeneration Wollastonite Antimicrobial activity Propolis
title_short	Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea
title_full	Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea
title_fullStr	Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea
title_full_unstemmed	Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea
title_sort	Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea
dc.creator.fl_str_mv	Moreno Florez, Ana Isabel
dc.contributor.advisor.none.fl_str_mv	García, Claudia Patricia Pelaez Vargas, Alejandro
dc.contributor.author.none.fl_str_mv	Moreno Florez, Ana Isabel
dc.contributor.financer.none.fl_str_mv	Minciencias Plan Bienal de Convocatorias 2019 Minciencias Convocatoria de proyectos que conectan el conocimiento - Proyecto 2019 852-2019. Número de contrato 80740-476-2020.
dc.contributor.researchgroup.spa.fl_str_mv	Materiales Cerámicos y Vítreos
dc.contributor.orcid.spa.fl_str_mv	Moreno Florez, Ana Isabel [0000-0003-2823-8822]
dc.contributor.cvlac.spa.fl_str_mv	MORENO FLOREZ, ANA ISABEL https://scienti.minciencias.gov.co/cvlac/jsp/report-index.jsp
dc.contributor.researchgate.spa.fl_str_mv	https://www.researchgate.net/profile/Isabel-Moreno-4
dc.subject.ddc.spa.fl_str_mv	600 - Tecnología (Ciencias aplicadas)::602 - Miscelánea 660 - Ingeniería química::666 - Cerámica y tecnologías afines 680 - Manufactura para usos específicos::686 - Imprenta y actividades relacionadas
topic	600 - Tecnología (Ciencias aplicadas)::602 - Miscelánea 660 - Ingeniería química::666 - Cerámica y tecnologías afines 680 - Manufactura para usos específicos::686 - Imprenta y actividades relacionadas Regeneración Ósea Osteomielitis Productos con Acción Antimicrobiana Ingeniería de Tejidos Própolis Antimicrobianos Antiinfecciosos Impresión Tridimensional Impresion 3D Regeneración ósea Actividad antimicrobiana Propóleos 3D printing Bone regeneration Wollastonite Antimicrobial activity Propolis
dc.subject.decs.none.fl_str_mv	Regeneración Ósea Osteomielitis Productos con Acción Antimicrobiana Ingeniería de Tejidos Própolis Antimicrobianos Antiinfecciosos Impresión Tridimensional
dc.subject.proposal.spa.fl_str_mv	Impresion 3D Regeneración ósea Actividad antimicrobiana Propóleos
dc.subject.proposal.eng.fl_str_mv	3D printing Bone regeneration Wollastonite Antimicrobial activity Propolis
description	ilustraciones, gráficos
publishDate	2023
dc.date.issued.none.fl_str_mv	2023
dc.date.accessioned.none.fl_str_mv	2024-05-08T18:45:43Z
dc.date.available.none.fl_str_mv	2024-05-08T18:45:43Z
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/86049
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/86049 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.indexed.spa.fl_str_mv	LaReferencia
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dc.rights.license.spa.fl_str_mv	Atribución-NoComercial 4.0 Internacional
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dc.format.extent.spa.fl_str_mv	174 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellin
dc.publisher.program.spa.fl_str_mv	Medellín - Ciencias - Doctorado en Biotecnología
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellín
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2García, Claudia Patriciad70ae25bb5cde065d68e8defb6f204b5Pelaez Vargas, Alejandro0424215de61b6e760a210221c3b44382Moreno Florez, Ana Isabel59b5b06b5d9108d39c55eed5ada7978dMinciencias Plan Bienal de Convocatorias 2019Minciencias Convocatoria de proyectos que conectan el conocimiento - Proyecto 2019 852-2019. Número de contrato 80740-476-2020.Materiales Cerámicos y VítreosMoreno Florez, Ana Isabel [0000-0003-2823-8822]MORENO FLOREZ, ANA ISABEL https://scienti.minciencias.gov.co/cvlac/jsp/report-index.jsphttps://www.researchgate.net/profile/Isabel-Moreno-42024-05-08T18:45:43Z2024-05-08T18:45:43Z2023https://repositorio.unal.edu.co/handle/unal/86049Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, gráficosUna alternativa para solucionar la problemática de los defectos es la manufactura aditiva, específicamente en el diseño y fabricación de dispositivos de reemplazamiento óseo. Sin embrago, la posibilidad de fallo debido a infecciones requiere mejoras en el potencial antimicrobiano de estos dispositivos. En esta tesis se propuso desarrollar por manufactura aditiva andamios antimicrobianos potencializados con extractos de propóleos para regeneración ósea. Se utilizó un diseño de experimentos factorial fraccional en la formulación de una pasta cerámica empleada como tinta de impresión 3D para fabricar andamios con geometría TPMS Gyroid, los cuales se impregnaron con extractos etanólicos de propóleos (EEP). Se evaluó la actividad antimicrobiana de los EEP y de los andamios impregnados con EEP (AI) frente a Staphylococcus aureus, Staphylococcus epidermidis y su co-cultivo midiendo zonas de inhibición y la viabilidad de biopelículas. En pruebas in vitro se evaluó la interacción de los andamios con cultivos celulares de osteosarcoma humano (SaOS) y células madre mesenquimales humanas (bmMSC) en términos de proliferación celular (Ensayo alamar blue) y actividad metabólica (Tinciones de Alizarin red, Von kossa y Actividad de la ALP). Los resultados mostraron que la manufactura aditiva posibilitó la fabricación de andamios cerámicos con geometrías complejas que se impregnaron exitosamente con extractos de propóleos provenientes de la Orinoquía Colombiana, los cuales mostraron inhibición considerable de las cepas estudiadas y reducción significativa en la viabilidad del co-cultivo por parte de los AI. La evaluación in vitro mostró una proliferación en los andamios superior al 90 % de las SaOS y cercano al 50 % de las bmMSC, además se encontraron zonas con señales de mineralización. En conclusión, los andamios fabricados son aptos para la reparación y regeneración ósea y presentan un potencial antibacteriano contra cepas relacionadas con el desarrollo de osteomielitis. (Tomado de la fuente)The problem of bone defects has been addressed through additive manufacturing, specifically with bone replacement devices. However, the potential for failure due to infection makes it necessary to improve the antimicrobial potential of these devices. The aim of this thesis is to develop antimicrobial scaffolds by additive manufacturing, enhanced with propolis extract for bone regeneration. A fractional factorial design of experiments was used to formulate a ceramic paste used as a 3D printing ink to fabricate scaffolds with TPMS gyroid geometry. These were impregnated with ethanolic extracts of propolis (EEP). The antimicrobial activity of EEP and EEP-impregnated scaffolds (AI) was evaluated against Staphylococcus aureus, Staphylococcus epidermidis and their co-cultures by measuring inhibition zones and biofilm viability. In vitro assays evaluated the interaction of the scaffolds with cultures of human osteosarcoma cells (SaOS) and human mesenchymal stem cells (bmMSC) in terms of cell proliferation (Alamar blue assay) and metabolic activity (Alizarin red, Von Kossa staining and ALP activity). The results showed that additive manufacturing made possible the fabrication of ceramic scaffolds with complex geometries that were successfully impregnated with propolis extracts from the Colombian Orinoco region, which showed considerable inhibition of the studied strains and significant reduction in the viability of the co-culture by the IAs. The in vitro evaluation showed a proliferation in the scaffolds higher than 90 % of the SaOS and close to 50 % of the bmMSC, in addition, areas with signs of mineralization were found. In conclusion, the fabricated scaffolds are suitable for bone repair and regeneration and present antibacterial potential against strains related to the development of osteomyelitis.Minciencias ColombiaUniversidad Nacional de ColombiaUniversidad Cooperativa de ColombiaUniversidad EafitLeibniz University HannoverDoctoradoDoctor en BiotecnologíaBiotecnología.Sede Medellín174 páginasapplication/pdfspaUniversidad Nacional de Colombia - Sede MedellinMedellín - Ciencias - Doctorado en BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín600 - Tecnología (Ciencias aplicadas)::602 - Miscelánea660 - Ingeniería química::666 - Cerámica y tecnologías afines680 - Manufactura para usos específicos::686 - Imprenta y actividades relacionadasRegeneración ÓseaOsteomielitisProductos con Acción AntimicrobianaIngeniería de TejidosPrópolisAntimicrobianosAntiinfecciososImpresión TridimensionalImpresion 3DRegeneración óseaActividad antimicrobianaPropóleos3D printingBone regenerationWollastoniteAntimicrobial activityPropolisProducción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración óseaProduction by additive manufacturing of scaffolds with antimicrobial properties from Propolis from Tame (Arauca) for bone regeneration.Trabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDLaReferenciaNickolas TL, Leonard MB, Shane E. 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Mater Des. 2019;165:107597.Potencialización de los propóleos obtenidos de la apicultura de Tame (Arauca) como agentes bactericidas y antimicrobianos en andamios para regeneración ósea.Minciencias Plan Bienal de Convocatorias 2019EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86049/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1116795956.2023.pdf1116795956.2023.pdfTesis de Doctorado en Biotecnologíaapplication/pdf5449571https://repositorio.unal.edu.co/bitstream/unal/86049/2/1116795956.2023.pdf4b4872217890ee2c1373e8fd787ea3feMD52THUMBNAIL1116795956.2023.pdf.jpg1116795956.2023.pdf.jpgGenerated Thumbnailimage/jpeg5207https://repositorio.unal.edu.co/bitstream/unal/86049/3/1116795956.2023.pdf.jpg0178874db35ba7fc61c7387b9e706c85MD53unal/86049oai:repositorio.unal.edu.co:unal/860492024-08-24 23:13:41.055Repositorio Institucional Universidad Nacional de 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Producción por manufactura aditiva de andamios con propiedades antimicrobianas a partir de Propóleos de Tame (Arauca) para regeneración ósea

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