Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos

ilustraciones, gráficas, tablas

Autores:: Salamanca Saavedra, Yeimy Cristina

Tipo de recurso:

Fecha de publicación:: 2022

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.none.fl_str_mv	Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos
dc.title.translated.eng.fl_str_mv	Peptide chimeras: a synthetic alternative to enhance the antimicrobial activity of peptides
title	Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos
spellingShingle	Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos 540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales Péptidos Antimicrobianos Antimicrobial Peptides Biomoléculas Biomolecules Inmunología molecular Molecular immunology Actividad antimicrobiana Quimeras SPPS Melitina Buforina II Actividad anticancerígena Antimicrobial activity Anticancer activity Chimeras SPPS Melittin Buforin II
title_short	Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos
title_full	Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos
title_fullStr	Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos
title_full_unstemmed	Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos
title_sort	Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos
dc.creator.fl_str_mv	Salamanca Saavedra, Yeimy Cristina
dc.contributor.advisor.none.fl_str_mv	Garcia Castañeda, Javier Eduardo
dc.contributor.author.none.fl_str_mv	Salamanca Saavedra, Yeimy Cristina
dc.subject.ddc.spa.fl_str_mv	540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
topic	540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales Péptidos Antimicrobianos Antimicrobial Peptides Biomoléculas Biomolecules Inmunología molecular Molecular immunology Actividad antimicrobiana Quimeras SPPS Melitina Buforina II Actividad anticancerígena Antimicrobial activity Anticancer activity Chimeras SPPS Melittin Buforin II
dc.subject.decs.spa.fl_str_mv	Péptidos Antimicrobianos Antimicrobial Peptides
dc.subject.lemb.spa.fl_str_mv	Biomoléculas Biomolecules Inmunología molecular
dc.subject.lemb.eng.fl_str_mv	Molecular immunology
dc.subject.proposal.spa.fl_str_mv	Actividad antimicrobiana Quimeras SPPS Melitina Buforina II Actividad anticancerígena
dc.subject.proposal.eng.fl_str_mv	Antimicrobial activity Anticancer activity Chimeras SPPS Melittin Buforin II
description	ilustraciones, gráficas, tablas
publishDate	2022
dc.date.issued.none.fl_str_mv	2022
dc.date.accessioned.none.fl_str_mv	2023-03-06T20:51:25Z
dc.date.available.none.fl_str_mv	2023-03-06T20:51:25Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/83594
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/83594 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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spelling	Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Garcia Castañeda, Javier Eduardoc4db67db1b9848bd0eca4f5e0282f792600Salamanca Saavedra, Yeimy Cristinae6b742ca6208b5a5cb564a8d215f09b62023-03-06T20:51:25Z2023-03-06T20:51:25Z2022https://repositorio.unal.edu.co/handle/unal/83594Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, gráficas, tablasLa emergencia provocada por la resistencia microbiana a los medicamentos disponibles actualmente ha motivado el desarrollo de agente terapéuticos que superen esta limitación. Los péptidos antimicrobianos (PAMs) son una alternativa que ha ganado relevancia ya que poseen actividad antimicrobiana contra un amplio espectro de microorganismos y, anticancerígena contra líneas celulares de diferentes tipos de cáncer a través de mecanismos de acción únicos. Una de las alternativas que permite potenciar la actividad biológica de los PAMs es la síntesis de quimeras peptídicas, que permite la obtención de moléculas que no son producidas de forma natural y actúan a través de mecanismos de acción combinados generando aumento de la selectividad y actividad biológica entre 1,5 y 10 veces. En este sentido, el diseño de las quimeras puede combinar péptidos penetrantes en las células con péptidos activos biológicamente para generar péptidos híbridos con doble función. Dentro de la revisión bibliográfica se incluyen también diferentes métodos de obtención de PAMs como la obtención a partir de fuentes naturales, que se considera costosa y de baja aplicación en el campo industrial, la producción a través del método recombinante mediante la manipulación genética de microorganismos como E. Coli que se convierten en productores de PAMs, lo que lo hace un método económico y rápido, aunque presenta limitaciones en el diseño de secuencias con AA no naturales, y por último el método de síntesis química en fase sólida (SPPS) que es el de mayor popularidad ya que permite obtener péptidos sin limitaciones en el diseño de las secuencias, es un método escalable y que genera altos rendimientos. (Texto tomado de la fuente).The emergence caused by microbial resistance to currently available drugs has motivated the development of therapeutic agents that overcome this limitation. Antimicrobial peptides (AMPs) are an alternative that has gained relevance because they have antimicrobial activity against a wide spectrum of microorganisms and anticancer activity against cell lines of different types of cancer through unique mechanisms of action. One of the alternatives that allows enhancing the biological activity of PAMs is synthesis of peptide chimeras, which allows obtaining molecules that are not produced naturally and act through combined mechanisms of action, generating an increase in selectivity and biological activity. between 1.5 and 10 times. In this regard, the design of chimeras can combine cell-penetrating peptides with biologically active peptides to generate hybrid peptides with dual functions. Within the bibliographic review, different methods of obtaining PAMs are also included, such as obtaining from natural sources, which is considered expensive and of low application in the industrial field, production through the recombinant method through the genetic manipulation of microorganisms such as E. Coli that become producers of PAMs, which makes it an economical and fast method, although it has limitations in the design of sequences with non-natural AA, and finally the solid phase chemical synthesis method (SPPS) that is the most popular since it allows obtaining peptides without limitations in the design of the sequences, it is a scalable method that generates high yields.MaestríaMagíster en Ciencias - Químicaxiv, 120 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materialesPéptidos AntimicrobianosAntimicrobial PeptidesBiomoléculasBiomoleculesInmunología molecularMolecular immunologyActividad antimicrobianaQuimerasSPPSMelitinaBuforina IIActividad anticancerígenaAntimicrobial activityAnticancer activityChimerasSPPSMelittinBuforin IIQuimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidosPeptide chimeras: a synthetic alternative to enhance the antimicrobial activity of peptidesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMM. 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Li, «Recombinant production of antimicrobial peptides in Escherichia coli: a review,» Protein Expression and Purification, vol. 80, nº 2, pp. 260-267, 2011Público generalORIGINAL1026283492-2022.pdf1026283492-2022.pdfMaestría en Ciencias - Químicaapplication/pdf1744610https://repositorio.unal.edu.co/bitstream/unal/83594/4/1026283492-2022.pdfb28b474fae76fd31a9f7f04de86f2c38MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83594/5/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD55unal/83594oai:repositorio.unal.edu.co:unal/835942023-03-06 15:56:22.31Repositorio Institucional Universidad Nacional de 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

Quimeras peptídicas: una alternativa sintética para potenciar la actividad antimicrobiana de péptidos

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