Generation and evaluation of agbiogeneric glyphosate tolerant soybean plants

Ilustraciones a color, diagramas, fotografías

Autores:
Mora Oberlaender, Julián Oliverio
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2024
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
eng
OAI Identifier:
oai:repositorio.unal.edu.co:unal/86464
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/86464
https://repositorio.unal.edu.co
Palabra clave:
570 - Biología::576 - Genética y evolución
630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación
630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
Herbicida
Transformación genética
Cultivo de tejidos
GM crops
Herbicides
Intellectual Property
Genetic transformation
Plant tissue culture
Selection
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_908a437a6e87a7fc8b8cd9c12f8f1ea3
oai_identifier_str oai:repositorio.unal.edu.co:unal/86464
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.eng.fl_str_mv Generation and evaluation of agbiogeneric glyphosate tolerant soybean plants
dc.title.translated.spa.fl_str_mv Generación y evaluación de plantas de soya agrobiogenéricas tolerantes al glifosato
title Generation and evaluation of agbiogeneric glyphosate tolerant soybean plants
spellingShingle Generation and evaluation of agbiogeneric glyphosate tolerant soybean plants
570 - Biología::576 - Genética y evolución
630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación
630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
Herbicida
Transformación genética
Cultivo de tejidos
GM crops
Herbicides
Intellectual Property
Genetic transformation
Plant tissue culture
Selection
title_short Generation and evaluation of agbiogeneric glyphosate tolerant soybean plants
title_full Generation and evaluation of agbiogeneric glyphosate tolerant soybean plants
title_fullStr Generation and evaluation of agbiogeneric glyphosate tolerant soybean plants
title_full_unstemmed Generation and evaluation of agbiogeneric glyphosate tolerant soybean plants
title_sort Generation and evaluation of agbiogeneric glyphosate tolerant soybean plants
dc.creator.fl_str_mv Mora Oberlaender, Julián Oliverio
dc.contributor.advisor.none.fl_str_mv López Carrascal, Camilo Ernesto
dc.contributor.author.none.fl_str_mv Mora Oberlaender, Julián Oliverio
dc.contributor.researchgroup.spa.fl_str_mv Ingeniería Genética de Plantas
dc.contributor.orcid.spa.fl_str_mv Mora Oberlaender, Julián Oliverio [0000-0003-0304-2380]
dc.contributor.cvlac.spa.fl_str_mv Mora Oberlaender, Julián Oliverio [0001426306]
dc.contributor.scopus.spa.fl_str_mv Mora Oberlaender, Julián Oliverio [55918882500]
dc.contributor.researchgate.spa.fl_str_mv Mora-Oberlaender, Julian
dc.contributor.googlescholar.spa.fl_str_mv Mora Oberlaender, Julian
dc.subject.ddc.spa.fl_str_mv 570 - Biología::576 - Genética y evolución
630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación
630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
topic 570 - Biología::576 - Genética y evolución
630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación
630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
Herbicida
Transformación genética
Cultivo de tejidos
GM crops
Herbicides
Intellectual Property
Genetic transformation
Plant tissue culture
Selection
dc.subject.agrovoc.none.fl_str_mv Herbicida
Transformación genética
Cultivo de tejidos
dc.subject.proposal.eng.fl_str_mv GM crops
Herbicides
Intellectual Property
Genetic transformation
Plant tissue culture
Selection
description Ilustraciones a color, diagramas, fotografías
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-07-16T17:28:53Z
dc.date.available.none.fl_str_mv 2024-07-16T17:28:53Z
dc.date.issued.none.fl_str_mv 2024
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TD
format http://purl.org/coar/resource_type/c_db06
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/86464
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co
url https://repositorio.unal.edu.co/handle/unal/86464
https://repositorio.unal.edu.co
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.indexed.spa.fl_str_mv Agrovoc
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2López Carrascal, Camilo Ernesto8af146341b8748e015282a47eef1e819Mora Oberlaender, Julián Oliverio0e977d6d11a0d65bee71e50c47d5f2bdIngeniería Genética de PlantasMora Oberlaender, Julián Oliverio [0000-0003-0304-2380]Mora Oberlaender, Julián Oliverio [0001426306]Mora Oberlaender, Julián Oliverio [55918882500]Mora-Oberlaender, JulianMora Oberlaender, Julian2024-07-16T17:28:53Z2024-07-16T17:28:53Z2024https://repositorio.unal.edu.co/handle/unal/86464Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.coIlustraciones a color, diagramas, fotografíasSoybean is one of the main crops worldwide to which biotechnology has contributed greatly since the first genetically modified, herbicide-tolerant crops were introduced. In particular, glyphosate tolerance facilitates soybean production by reducing inputs, environmental impact and the need for tillage. First generation glyphosate tolerance technology is now in the public domain and therefore creates an opportunity for the development of agbiogeneric soybean. In Colombia, this can contribute in reducing the dependence on imported soybean by boosting competitiveness. This work contributes to the development of agbiogeneric glyphosate-tolerant soybeans by furthering the phenotypic and molecular evaluation and characterization of potential transgenic events. Colombian soybean varieties Brasilera 1, Brasilera 2 and FNS 01 were subjected to transformation, regeneration and selection, together with variety Soy-SK7 which had been included in previous work. Twenty-one potential primary transformants were obtained after adjusting in vitro selection and regeneration. A workflow was established for the obtention of subsequent generations derived from primary transformants and for their molecular evaluation via PCR and phenotypic selection using different doses of glyphosate. Tolerance to this herbicide was linked to the presence of an optimized version of the cp4epsps transgene in transformed plants. The results obtained here have helped identify bottlenecks in the generation of potentially transformed events and have highlighted the need for more efficient transformation protocols. Phenotypic and molecular selection requires a pool from which to identify those lines with more promising characteristics.La soya es uno de los principales cultivos en los que la biotecnología ha contribuido desde la introducción de los primeros cultivos transgénicos tolerantes a herbicidas. En particular la tolerancia al glifosato facilita la producción al reducir insumos, impacto ambiental y labranza. La primera generación de la tecnología está ahora en el dominio público, lo que crea una oportunidad para el desarrollo de soya agrobiogenérica. En Colombia esto contribuye a reducir la dependencia de las importaciones al impulsar la competitividad de este cultivo. Este trabajo contribuye al desarrollo de soya agrobiogenérica tolerante al glifosato mediante la evaluación fenotípica y molecular y la caracterización de eventos potenciales. Las variedades de soya colombianas Brasilera 1, Brasilera 2 y FNS 01 fueron sometidas a transformación, regeneración y selección, junto con la variedad Soy-SK7 que había sido incluida en trabajos previos. Se obtuvieron veintiún posibles transformantes primarios después de ajustar la selección in vitro y la regeneración. Se estableció un flujo de trabajo para la obtención de generaciones posteriores derivadas de los transformantes primarios y para su evaluación molecular mediante PCR y selección fenotípica utilizando diferentes dosis de glifosato. La tolerancia a este herbicida se asoció a la presencia de una versión optimizada del gen cp4epsps en las plantas transformadas. Los resultados obtenidos aquí han ayudado a identificar obstáculos en la generación de eventos potencialmente transformados y han destacado la necesidad de protocolos de transformación más eficientes. La selección fenotípica y molecular requiere de un conjunto del cual identificar aquellas líneas con características más prometedoras. (Texto tomado de la fuente)DoctoradoDoctor en BiotecnologíaBiotecnología Agrícolaxxv, 91 páginasapplication/pdfengUniversidad Nacional de ColombiaBogotá - Ciencias - Doctorado en BiotecnologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::576 - Genética y evolución630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materialesHerbicidaTransformación genéticaCultivo de tejidosGM cropsHerbicidesIntellectual PropertyGenetic transformationPlant tissue cultureSelectionGeneration and evaluation of agbiogeneric glyphosate tolerant soybean plantsGeneración y evaluación de plantas de soya agrobiogenéricas tolerantes al glifosatoTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAgrovocAgbioInvestor. 2024. 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