Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos

ilustraciones

Autores:
Gaete Carrillo, Paula Valentina
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
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spa
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oai:repositorio.unal.edu.co:unal/83542
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https://repositorio.unal.edu.co/handle/unal/83542
https://repositorio.unal.edu.co/
Palabra clave:
610 - Medicina y salud
Macrófagos
Fagocitosis
Macrophages
Phagocytosis
Ppard
Metabolismo
Lípidos
Colesterol
Ateroesclerosis
Inflamación
Ppard
Metabolism
Lipids
Cholesterol
Atherosclerosis
Inflammation
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_894454d979831adce24495a2e630e0ec
oai_identifier_str oai:repositorio.unal.edu.co:unal/83542
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos
dc.title.translated.eng.fl_str_mv Role of the nuclear receptor PPARβ/δ in the metabolic response to medium-chain fatty acids in murine macrophages
title Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos
spellingShingle Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos
610 - Medicina y salud
Macrófagos
Fagocitosis
Macrophages
Phagocytosis
Ppard
Metabolismo
Lípidos
Colesterol
Ateroesclerosis
Inflamación
Ppard
Metabolism
Lipids
Cholesterol
Atherosclerosis
Inflammation
title_short Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos
title_full Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos
title_fullStr Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos
title_full_unstemmed Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos
title_sort Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos
dc.creator.fl_str_mv Gaete Carrillo, Paula Valentina
dc.contributor.advisor.none.fl_str_mv Mendivil Anaya, Carlos Olimpo
dc.contributor.author.none.fl_str_mv Gaete Carrillo, Paula Valentina
dc.contributor.researchgroup.spa.fl_str_mv Diabetes, Lípidos y Metabolismo UniAndes
dc.contributor.orcid.spa.fl_str_mv Paula Valentina Gaete Carrillo, 0000-0001-9387-6017
dc.contributor.cvlac.spa.fl_str_mv https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000097625
dc.contributor.researchgate.spa.fl_str_mv https://www.researchgate.net/profile/Paula-Gaete
dc.contributor.googlescholar.spa.fl_str_mv https://scholar.google.com/citations?user=Wd5n4WQAAAAJ&hl=es&authuser=1
dc.subject.ddc.spa.fl_str_mv 610 - Medicina y salud
topic 610 - Medicina y salud
Macrófagos
Fagocitosis
Macrophages
Phagocytosis
Ppard
Metabolismo
Lípidos
Colesterol
Ateroesclerosis
Inflamación
Ppard
Metabolism
Lipids
Cholesterol
Atherosclerosis
Inflammation
dc.subject.decs.spa.fl_str_mv Macrófagos
Fagocitosis
dc.subject.decs.eng.fl_str_mv Macrophages
Phagocytosis
dc.subject.proposal.spa.fl_str_mv Ppard
Metabolismo
Lípidos
Colesterol
Ateroesclerosis
Inflamación
dc.subject.proposal.eng.fl_str_mv Ppard
Metabolism
Lipids
Cholesterol
Atherosclerosis
Inflammation
description ilustraciones
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-02-22T15:39:50Z
dc.date.available.none.fl_str_mv 2023-02-22T15:39:50Z
dc.date.issued.none.fl_str_mv 2023-02-21
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/83542
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/83542
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.format.extent.spa.fl_str_mv xvi, 85 páginas
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv Bogotá - Medicina - Maestría en Bioquímica
dc.publisher.faculty.spa.fl_str_mv Facultad de Medicina
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Mendivil Anaya, Carlos Olimpo1fad3bd3a39fe755221011993c12ff93Gaete Carrillo, Paula Valentina4b23097cf854561ccc6b550a9ac0d2d3Diabetes, Lípidos y Metabolismo UniAndesPaula Valentina Gaete Carrillo, 0000-0001-9387-6017https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000097625https://www.researchgate.net/profile/Paula-Gaetehttps://scholar.google.com/citations?user=Wd5n4WQAAAAJ&hl=es&authuser=12023-02-22T15:39:50Z2023-02-22T15:39:50Z2023-02-21https://repositorio.unal.edu.co/handle/unal/83542Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustracionesPapel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos Objetivo: El objetivo de esta investigación es esclarecer la participación del receptor nuclear PPARβ/δ en la respuesta metabólica que desarrollan los macrófagos de ratón después de la exposición a ácidos grasos de cadena media (octanoico, C8:0 y decanoico, C10:0). Metodología: Se cultivaron macrófagos murinos J774A.1 en diferentes condiciones de tratamiento; en la mitad de las células se realizó un knock-down de la expresión para PPARβ/δ con un sistema de ácido ribonucleico pequeño de interferencia y el porcentaje de interferencia obtenido se verificó por RT-qPCR. El diseño experimental comparó 5 factores en las células a estudio: - Knockdown de PPARβ/δ (sí o no) - Exposición a ácido graso (cadena media vs cadena larga) - Exposición a un agonista de PPARβ/δ (GW501516) - Activación por lipopolisacárido (sí o no) - Control negativo En cada grupo de células se evaluó la expresión de genes relacionados con el metabolismo lipídico (Scarb1, Plin2, Npc1, Pltp, Cpt1, Abca1 y Abcg1) y con la función inmunitaria (Il6, Tnf, Ifng, Il10, Nos2, Ptgs2, Mcp1y Mmp9) mediante RT-qPCR. La expresión de cada gen se cuantificó de forma relativa al gen de la gliceraldeheido-3-fosfato deshidrogenasa utilizando el método ∆∆Ct. Para evaluar el impacto funcional de la exposición a ácidos grasos de cadena media sobre la fagocitosis de lipoproteínas, un paso fundamental de la aterogénesis, se realizó en cada subgrupo de tratamientos un ensayo de captación de lipoproteínas de baja densidad (LDL) fluorescentemente marcadas. Resultados y conclusiones: Este estudio amplió el conocimiento sobre la respuesta a los ácidos grasos de cadena media en la expresión de genes relacionados con el metabolismo de lípidos y la función inmune en macrófagos murinos, y en qué medida este efecto está mediado por el receptor nuclear PPARβ/δ. Palabras clave: Ppard, metabolismo, lípidos, colesterol, ateroesclerosis e inflamación. (Texto tomado de la fuente)Role of the nuclear receptor PPARβ/δ in the metabolic response to medium-chain fatty acids in murine macrophages Aim: The aim of this investigation is to clarify the role of the nuclear receptor PPARβ/δ in the metabolic response that the murine macrophages develop after the exposition to medium-chain fatty acids (octanoic acid, C8:0 y decanoic acid, C10:0). Methods: J774A.1 murine macrophages were cultured under different treatment conditions; Half of the cells, had a knock-down against PPARβ/δ by using small interfering ribonucleic acids and the percentage of reduced expression was verified by RT-qPCR. The experimental design compared 5 factors in the cells under study: - PPARβ/δ knockdown (yes or no) - Exposure to fatty acid (medium chain vs. long chain) - Exposure to a PPARβ/δ agonist (GW501516) - Activation by lipopolysaccharide (yes or no) - Negative control The expression of genes related to lipid metabolism (Scarb1, Plin2, Npc1, Pltp, Cpt1, Abca1 and Abcg1) and immune function (Il6, Tnf, Ifng, Il10, Nos2, Ptgs2, Mcp1 and Mmp9) was evaluated in each group of cells by RT-qPCR. The expression of each gene was quantified relative to the glyceraldehyde-3-phosphate dehydrogenase gene using the ∆∆Ct method. To assess the functional impact of medium-chain fatty acid exposure on lipoprotein phagocytosis, a critical step in atherogenesis, a fluorescently labeled low-density lipoprotein (LDL) uptake assay was performed on each treatment subgroup. Conclusions and results: This study furthered our understanding of the response to medium-chain fatty acids in the expression of genes related to lipid metabolism and immune function in murine macrophages, and clarify the role of the nuclear receptor PPARβ/δ in this process. Key words: Ppard, metabolism, lipids, cholesterol, atherosclerosis and inflammation.MaestríaMagíster en BioquímicaMetabolismoxvi, 85 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina - Maestría en BioquímicaFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y saludMacrófagosFagocitosisMacrophagesPhagocytosisPpardMetabolismoLípidosColesterolAteroesclerosisInflamaciónPpardMetabolismLipidsCholesterolAtherosclerosisInflammationPapel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinosRole of the nuclear receptor PPARβ/δ in the metabolic response to medium-chain fatty acids in murine macrophagesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMZhao, X., Wang, J., Deng, Y., Liao, L., Zhou, M., Peng, C., & Li, Y. (2021). 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Nucleic acids research, 43(10), 5033-5051.Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinosMito Therapies SASLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83542/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1026590616.2023.pdf1026590616.2023.pdfTesis de Maestría en Ciencias - Bioquímicaapplication/pdf4637937https://repositorio.unal.edu.co/bitstream/unal/83542/2/1026590616.2023.pdfc404c3cd259e72f340378ef179c9d2b7MD52THUMBNAIL1026590616.2023.pdf.jpg1026590616.2023.pdf.jpgGenerated Thumbnailimage/jpeg5432https://repositorio.unal.edu.co/bitstream/unal/83542/3/1026590616.2023.pdf.jpge34599515c34d9f300a2b77e389e9f92MD53unal/83542oai:repositorio.unal.edu.co:unal/835422024-07-18 23:11:16.589Repositorio Institucional Universidad Nacional de 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