Protopectinase-se from Geotrichumklebahnii: Studies of the adsorption and pectin-solubilization capacity

Protopectinase SE (PPase-SE) is a polygalacturonase produced by Geotrichumklebahnii with the capacity to liberate pectin through protopectin hydrolysis. The protopectin cleavage is a typical heterogeneous-catalysis reaction whose interaction between the enzyme and the protopectin substrate from lemo...

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Autores:: Zapata Zapata, Arley David
Hours, Roque Alberto
Cavalitto, Sebastian Fernando

Tipo de recurso:: Article of journal

Fecha de publicación:: 2017

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

Description
Summary:	Protopectinase SE (PPase-SE) is a polygalacturonase produced by Geotrichumklebahnii with the capacity to liberate pectin through protopectin hydrolysis. The protopectin cleavage is a typical heterogeneous-catalysis reaction whose interaction between the enzyme and the protopectin substrate from lemon albedo along with the release of the pectin-reaction product were the objectives of this investigation. The interaction between PPase-SE and protopectin depended on the particle size and the structure of the substrate as well as on the nature of the buffer. The adsorption kinetics follows, for small particles, a Langmuir isotherm pattern. The reaction exhibited Michaelis-Menten kinetics, giving respective apparent-Km and Vmax values of 30.2 g/l and 57.3 g/l.h. The better results in enzyme adsorption and pectin releasing were obtained with citrate and citrate-phosphate buffers. This report constitutes the first investigation in pectin solubilization involving a model for the substrate-binding mechanism within the pectinase-protopectinase system.

Protopectinase-se from Geotrichumklebahnii: Studies of the adsorption and pectin-solubilization capacity

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