Exploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesos

ilustraciones, diagramas

Autores:
Vásquez Restrepo, Andrés
Tipo de recurso:
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/83418
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/83418
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología::572 - Bioquímica
Biotechnology
Electrochemistry
Biochemistry
Electroquímica
Biotecnología
Bioquímica
Omics
In silico
Ingeniería metabólica
Diseño Racional de Bioprocesos
Biotermodinámica
Metabolic Engineering
Rational Design of Bioprocesses
Biothermodynamics
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openAccess
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Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_82dcca9817da77d9375ef98a1b74fc7a
oai_identifier_str oai:repositorio.unal.edu.co:unal/83418
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Exploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesos
dc.title.translated.eng.fl_str_mv In-silico exploration of Electro-fermentation strategies in the Rational Design of Bioprocesses
title Exploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesos
spellingShingle Exploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesos
570 - Biología::572 - Bioquímica
Biotechnology
Electrochemistry
Biochemistry
Electroquímica
Biotecnología
Bioquímica
Omics
In silico
Ingeniería metabólica
Diseño Racional de Bioprocesos
Biotermodinámica
Metabolic Engineering
Rational Design of Bioprocesses
Biothermodynamics
title_short Exploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesos
title_full Exploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesos
title_fullStr Exploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesos
title_full_unstemmed Exploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesos
title_sort Exploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesos
dc.creator.fl_str_mv Vásquez Restrepo, Andrés
dc.contributor.advisor.none.fl_str_mv SUAREZ-MENDEZ, CAMILO
dc.contributor.author.none.fl_str_mv Vásquez Restrepo, Andrés
dc.contributor.researchgroup.spa.fl_str_mv Bioprocesos y Flujos Reactivos
dc.contributor.orcid.spa.fl_str_mv Vasquez-Restrepo, Andres [0000-0001-9627-1005]
Suárez Méndez, Camilo[0000-0002-5345-9662]
dc.contributor.cvlac.spa.fl_str_mv https://scienti.colciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000122302
dc.subject.ddc.spa.fl_str_mv 570 - Biología::572 - Bioquímica
topic 570 - Biología::572 - Bioquímica
Biotechnology
Electrochemistry
Biochemistry
Electroquímica
Biotecnología
Bioquímica
Omics
In silico
Ingeniería metabólica
Diseño Racional de Bioprocesos
Biotermodinámica
Metabolic Engineering
Rational Design of Bioprocesses
Biothermodynamics
dc.subject.lemb.eng.fl_str_mv Biotechnology
Electrochemistry
Biochemistry
dc.subject.lemb.spa.fl_str_mv Electroquímica
Biotecnología
Bioquímica
dc.subject.proposal.spa.fl_str_mv Omics
In silico
Ingeniería metabólica
Diseño Racional de Bioprocesos
Biotermodinámica
dc.subject.proposal.eng.fl_str_mv Metabolic Engineering
Rational Design of Bioprocesses
Biothermodynamics
description ilustraciones, diagramas
publishDate 2022
dc.date.issued.none.fl_str_mv 2022
dc.date.accessioned.none.fl_str_mv 2023-02-10T16:33:23Z
dc.date.available.none.fl_str_mv 2023-02-10T16:33:23Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/83418
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/83418
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.indexed.spa.fl_str_mv RedCol
LaReferencia
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spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2SUAREZ-MENDEZ, CAMILO0cb894551eb9c1f9737a27b5a0b26d87600Vásquez Restrepo, Andrés3c1b08f3bb6101be643086b4f7ed2c3d600Bioprocesos y Flujos ReactivosVasquez-Restrepo, Andres [0000-0001-9627-1005]Suárez Méndez, Camilo[0000-0002-5345-9662]https://scienti.colciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=00001223022023-02-10T16:33:23Z2023-02-10T16:33:23Z2022https://repositorio.unal.edu.co/handle/unal/83418Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasLa electro-fermentación es una estrategia emergente para optimizar los bioprocesos al regular el balance redox intracelular y redireccionar los flujos metabólicos. En el presente trabajo se evaluó in silico la electro-fermentación desde el marco del diseño racional de bioprocesos para determinar su efecto en el aprovechamiento de la energía biológicamente disponible y los rendimientos del proceso. Para lo cual, se desarrolló una metodología que permitió estimar sus costos energéticos asociados y evaluar su capacidad de redireccionamiento metabólico. Se definieron un conjunto de semirreacciones que permitieron utilizar los principios de la electroquímica para establecer los requerimientos energéticos del proceso, junto con su modelo de caja negra. Se encontró que la energía libre de Gibbs de reacción depende del voltaje aplicado y el potencial de reducción de la molécula aceptora interna de electrones. Además, se planteó un modelo metabólico que incluyó el transporte extracelular de electrones y permitió evidenciar los diferentes cambios metabólicos al cambiar el balance redox a través de la interacción con el electrodo. Finalmente, se evaluaron diferentes casos de estudio para evidenciar el desempeño de la metodología desarrollada, en donde se logró solucionar déficits de ATP y electrones a expensas de una pequeña desviación de carbonos hacia subproductos debido a la generación de un desbalance redox en el metabolismo celular. La presente metodología representa un primer intento de una estimación in silico de los requerimientos de corriente eléctrica y voltaje asociados a una electro-fermentación a partir de fundamentos teóricos. (Texto tomado de la fuente)Electro-fermentation is a novel strategy for optimizing bioprocesses in which the intracellular redox balance is regulated to redirect the carbon metabolic flux towards a desired product. In this work, an in-silico evaluation of the electro-fermentation has been made within the frame of a methodology referred to as Rational Design of Bioprocesses to evaluate its effects on microbial bioenergetics and process performance. Here, a new methodology is proposed for estimating the associated Gibbs energy costs, the development of a black-box model and the evaluation of its capacity to redirect metabolic fluxes. A set of semi reactions are used to describe the interactions between the electrode and the microbe, where the Gibbs energy involved in the electro-fermentation process is associated to the electrode’s poised voltage and the standard reduction potential of the internal electron acceptor. Besides, a new metabolic model is developed incorporating a set of reactions for the extracellular electron transfer mechanism. It has been proven that metabolic changes occur by an unbalanced NADH pool generated by the interaction of the microbe with a poised electrode. Finally, both thermodynamic and metabolic models are used in different study cases to evaluate the performance of the complete developed framework for electro-fermentations, where it has been proven that it can be used to solve ATP deficits in metabolic networks. To my knowledge, it is the first attempt of an in-silico based theorical framework to describe the energy, current and voltage associated with electro-fermentations.MaestríaMagister en Ciencias - BiotecnologíaDiseño Racional de BioprocesosÁrea Curricular de Bioctecnología210 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín570 - Biología::572 - BioquímicaBiotechnologyElectrochemistryBiochemistryElectroquímicaBiotecnologíaBioquímicaOmicsIn silicoIngeniería metabólicaDiseño Racional de BioprocesosBiotermodinámicaMetabolic EngineeringRational Design of BioprocessesBiothermodynamicsExploración in silico de estrategias de electro-fermentación en el diseño racional de bioprocesosIn-silico exploration of Electro-fermentation strategies in the Rational Design of BioprocessesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMRedColLaReferenciaL. 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Biofuels, vol. 9, no. 1, pp. 1–12, 2016, doi: 10.1186/s13068-016-0663-2.EstudiantesInvestigadoresORIGINAL1017236136_2022.pdf1017236136_2022.pdfTesis de Maestría en Ciencias - Biotecnologíaapplication/pdf2136850https://repositorio.unal.edu.co/bitstream/unal/83418/2/1017236136_2022.pdf6245516e50c51bec90a88cca0c814944MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83418/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51THUMBNAIL1017236136_2022.pdf.jpg1017236136_2022.pdf.jpgGenerated Thumbnailimage/jpeg4818https://repositorio.unal.edu.co/bitstream/unal/83418/3/1017236136_2022.pdf.jpg04c2ad7ca0e74c40988af946f7231757MD53unal/83418oai:repositorio.unal.edu.co:unal/834182024-08-17 23:13:26.979Repositorio Institucional Universidad Nacional de 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