Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)

ilustraciones, fotografías, mapas

Autores:: Cuello Mejia, Mishelle

Tipo de recurso:

Fecha de publicación:: 2022

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)
dc.title.translated.eng.fl_str_mv	Epidemiological tracing of the genomic profiles of antibiotic resistance in clinical isolates of P. aeruginosa using Whole Genome Sequencing (WGS)
title	Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)
spellingShingle	Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS) 610 - Medicina y salud 000 - Ciencias de la computación, información y obras generales Bacterias Bacteria Epidemiological Monitoring Monitoreo epidemiológico Pseudomonas aeruginosa Infecciones Asociadas a la Atención en Salud Resistencia a antibióticos Seguimiento epidemiológico Secuenciación de Genoma Completo Grupos clonales Secuencio-tipos (ST) Healthcare-Associated Infections (HAIs) Antibiotic resistance Epidemiological tracing Whole Genome Sequencing (WGS) Sequence-types (ST) Clonal groups
title_short	Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)
title_full	Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)
title_fullStr	Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)
title_full_unstemmed	Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)
title_sort	Seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)
dc.creator.fl_str_mv	Cuello Mejia, Mishelle
dc.contributor.advisor.none.fl_str_mv	Barreto Hernández, Emiliano Reguero Reza, María Teresa Jesús
dc.contributor.author.none.fl_str_mv	Cuello Mejia, Mishelle
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Bioinformática Grupo de Epidemiología Molecular
dc.subject.ddc.spa.fl_str_mv	610 - Medicina y salud 000 - Ciencias de la computación, información y obras generales
topic	610 - Medicina y salud 000 - Ciencias de la computación, información y obras generales Bacterias Bacteria Epidemiological Monitoring Monitoreo epidemiológico Pseudomonas aeruginosa Infecciones Asociadas a la Atención en Salud Resistencia a antibióticos Seguimiento epidemiológico Secuenciación de Genoma Completo Grupos clonales Secuencio-tipos (ST) Healthcare-Associated Infections (HAIs) Antibiotic resistance Epidemiological tracing Whole Genome Sequencing (WGS) Sequence-types (ST) Clonal groups
dc.subject.decs.spa.fl_str_mv	Bacterias
dc.subject.decs.eng.fl_str_mv	Bacteria Epidemiological Monitoring
dc.subject.decs.epa.fl_str_mv	Monitoreo epidemiológico
dc.subject.proposal.spa.fl_str_mv	Pseudomonas aeruginosa Infecciones Asociadas a la Atención en Salud Resistencia a antibióticos Seguimiento epidemiológico Secuenciación de Genoma Completo Grupos clonales Secuencio-tipos (ST)
dc.subject.proposal.eng.fl_str_mv	Healthcare-Associated Infections (HAIs) Antibiotic resistance Epidemiological tracing Whole Genome Sequencing (WGS) Sequence-types (ST) Clonal groups
description	ilustraciones, fotografías, mapas
publishDate	2022
dc.date.issued.none.fl_str_mv	2022-10-07
dc.date.accessioned.none.fl_str_mv	2023-07-07T13:55:45Z
dc.date.available.none.fl_str_mv	2023-07-07T13:55:45Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/84156
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/84156 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.rights.license.spa.fl_str_mv	Atribución-NoComercial-SinDerivadas 4.0 Internacional
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Maestría en Ciencias - Microbiología
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá,Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Barreto Hernández, Emilianob7a2cae2c08b5d6a549e173576c6c82dReguero Reza, María Teresa Jesús9ba54bc0326cdc978d84a9a9130f2d0cCuello Mejia, Mishelle3266d749cbd282158f458a03d0e4f619Grupo de BioinformáticaGrupo de Epidemiología Molecular2023-07-07T13:55:45Z2023-07-07T13:55:45Z2022-10-07https://repositorio.unal.edu.co/handle/unal/84156Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, mapasPseudomonas aeruginosa es uno de los principales microorganismos causantes de Infecciones Asociadas a la Atención en Salud, importante en el contexto epidemiológico actual debido a la emergencia de resistencia a los carbapenémicos. Teniendo en cuenta este planteamiento, el objetivo general del estudio consistió en realizar el seguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de P. aeruginosa mediante secuenciación de genoma completo (WGS). Para ello, se planteó un estudio prospectivo, descriptivo en una institución hospitalaria de tercer nivel de complejidad en Bogotá-Colombia, durante los años 2019 y 2020; se analizaron aislamientos clínicos de P. aeruginosa provenientes de pacientes internados en los servicios de UCI y hospitalización. Se obtuvieron 43 aislamientos provenientes de 32 pacientes, secuenciados utilizando las plataformas Illumina y Oxford Nanopore. En cuanto a los genomas analizados, se anotaron 95 genes diferentes mediadores de resistencia, encontrándose la presencia del gen blaKPC-3, este es el segundo reporte de blaKPC-3 en P. aeruginosa en Colombia. Se detectó la presencia 14 secuencio-tipos (ST), siendo el ST111 el más frecuente, adicionalmente se identificaron 7 nuevos ST que a la fecha no han sido reportados. A partir de un árbol de SNP (Single Nucleotide Polymorphism), se evidenció la presencia de 3 grupos clonales. En conclusión, la investigación epidemiológica y genómica combinada permitió identificar las variaciones intrapaciente a nivel de perfiles genómicos de resistencia y la diversidad genética de los aislamientos de P. aeruginosa, lo cual posibilitó establecer posibles rutas de transmisión entre pacientes vinculados por proximidad en periodos de tiempo y espacio. (Texto tomado de la fuente)Pseudomonas aeruginosa is one of the main microorganisms that cause healthcare-associated infections (HAIs), important in the current epidemiological context due to the emergence of resistance to carbapenems. Taking this into consideration, the general objective of the study was to perform the epidemiological tracing of the genomic profiles of antibiotic resistance in clinical isolates of P. aeruginosa using Whole Genome Sequencing (WGS). For this, a prospective and descriptive study was proposed in a third level hospital in Bogotá-Colombia, during 2019 and 2020; clinical isolates of P. aeruginosa from patients of ICU and hospitalization services were analyzed. 43 isolates from 32 patients were obtained and sequenced using the Illumina and Oxford Nanopore platforms. Regarding the analyzed genomes, 95 different resistance mediator genes were annotated, finding the blaKPC-3 gene which is the second report of blaKPC-3 in P. aeruginosa in Colombia. The presence of 14 sequence-types (ST) were identified, being the ST111 the most frequent; in addition, 7 new STs that have not been previously reported were identified in this study. From a SNP (Single Nucleotide Polymorphism) tree, the presence of 3 clonal groups were evidenced. In conclusion, the epidemiological and genomic research allowed to identify intra-patient variations of the genomic resistance profiles and the genetic diversity of the P. aeruginosa isolates, leading to establish possible routes of transmission between patients linked by proximity in periods of time and space.MaestríaMagíster en Ciencias - MicrobiologíaBiología molecular de agentes infecciosos124 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogotá,ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud000 - Ciencias de la computación, información y obras generalesBacteriasBacteriaEpidemiological MonitoringMonitoreo epidemiológicoPseudomonas aeruginosaInfecciones Asociadas a la Atención en SaludResistencia a antibióticosSeguimiento epidemiológicoSecuenciación de Genoma CompletoGrupos clonalesSecuencio-tipos (ST)Healthcare-Associated Infections (HAIs)Antibiotic resistanceEpidemiological tracingWhole Genome Sequencing (WGS)Sequence-types (ST)Clonal groupsSeguimiento epidemiológico de los perfiles genómicos de resistencia a antibióticos, en aislamientos clínicos de Pseudomonas aeruginosa mediante secuenciación de genoma completo (WGS)Epidemiological tracing of the genomic profiles of antibiotic resistance in clinical isolates of P. aeruginosa using Whole Genome Sequencing (WGS)Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbril D, Bravo-Ojeda J, García J, Leal -Castro A, Saavedra-Trujillo C, Madroñero J, Bustos R, Márquez-Ortiz R, Corredor-Rozo Z, Vanegas-Gómez N, E.-P. 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Antimicrobial Agents and Chemotherapy, 65(8). https://doi.org/10.1128/AAC.00773-21MincienciasEstudiantesInvestigadoresMaestrosORIGINAL1065651232.2023.pdf1065651232.2023.pdfTesis de Maestría en Ciencias - Microbiologíaapplication/pdf3677023https://repositorio.unal.edu.co/bitstream/unal/84156/4/1065651232.2023.pdf97844219dee4c3d88a696b58b53266faMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84156/5/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD55THUMBNAIL1065651232.2023.pdf.jpg1065651232.2023.pdf.jpgGenerated Thumbnailimage/jpeg5026https://repositorio.unal.edu.co/bitstream/unal/84156/6/1065651232.2023.pdf.jpg18e9acb8ea9c688bd4766f35edb17f82MD56unal/84156oai:repositorio.unal.edu.co:unal/841562023-08-10 23:04:22.161Repositorio Institucional Universidad Nacional de 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