Virulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en Colombia

ilustraciones, tablas

Autores:
Cerezo Cortés, María Irene
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2021
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/79870
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/79870
https://repositorio.unal.edu.co/
Palabra clave:
610 - Medicina y salud::616 - Enfermedades
Mycobacterium tuberculosis
Inmunidad
Immunity
Tuberculosis
Genotipo Beijing
Balb/c
Virulencia
Respuesta Inmune
Tuberculosis
Beijing-Genotype
Balb/c
Virulence
Immune Response
Rights
openAccess
License
Atribución-SinDerivadas 4.0 Internacional
id UNACIONAL2_7e57b1503b5faab3dfea819d95ce23d8
oai_identifier_str oai:repositorio.unal.edu.co:unal/79870
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Virulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en Colombia
dc.title.translated.eng.fl_str_mv Virulence, in vivo immune response and transcriptomics of Mycobacterium tuberculosis Beijing genotype circulating in Colombia
title Virulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en Colombia
spellingShingle Virulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en Colombia
610 - Medicina y salud::616 - Enfermedades
Mycobacterium tuberculosis
Inmunidad
Immunity
Tuberculosis
Genotipo Beijing
Balb/c
Virulencia
Respuesta Inmune
Tuberculosis
Beijing-Genotype
Balb/c
Virulence
Immune Response
title_short Virulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en Colombia
title_full Virulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en Colombia
title_fullStr Virulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en Colombia
title_full_unstemmed Virulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en Colombia
title_sort Virulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en Colombia
dc.creator.fl_str_mv Cerezo Cortés, María Irene
dc.contributor.advisor.none.fl_str_mv Murcia Aranguren, Martha Isabel
Hernández Pando, Rogelio Enrique
dc.contributor.author.none.fl_str_mv Cerezo Cortés, María Irene
dc.contributor.researchgroup.spa.fl_str_mv MICOBAC-UN
dc.contributor.subjectmatterexpert.none.fl_str_mv Rodríguez Juan Germán
dc.subject.ddc.spa.fl_str_mv 610 - Medicina y salud::616 - Enfermedades
topic 610 - Medicina y salud::616 - Enfermedades
Mycobacterium tuberculosis
Inmunidad
Immunity
Tuberculosis
Genotipo Beijing
Balb/c
Virulencia
Respuesta Inmune
Tuberculosis
Beijing-Genotype
Balb/c
Virulence
Immune Response
dc.subject.decs.none.fl_str_mv Mycobacterium tuberculosis
Inmunidad
Immunity
dc.subject.proposal.spa.fl_str_mv Tuberculosis
Genotipo Beijing
Balb/c
Virulencia
Respuesta Inmune
dc.subject.proposal.eng.fl_str_mv Tuberculosis
Beijing-Genotype
Balb/c
Virulence
Immune Response
description ilustraciones, tablas
publishDate 2021
dc.date.accessioned.none.fl_str_mv 2021-07-29T22:41:59Z
dc.date.available.none.fl_str_mv 2021-07-29T22:41:59Z
dc.date.issued.none.fl_str_mv 2021-07-27
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TD
format http://purl.org/coar/resource_type/c_db06
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/79870
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/79870
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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Derechos reservados al autor, 2021
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dc.format.extent.spa.fl_str_mv 320 páginas
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.coverage.country.none.fl_str_mv Colombia
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Ciudad de México
dc.publisher.program.spa.fl_str_mv Bogotá - Medicina - Doctorado en Ciencias Biomédicas
dc.publisher.faculty.spa.fl_str_mv Facultad de Medicina
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
institution Universidad Nacional de Colombia
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spelling Atribución-SinDerivadas 4.0 InternacionalDerechos reservados al autor, 2021http://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Murcia Aranguren, Martha Isabel2d3a40be45d44dfb2c92cd107332178cHernández Pando, Rogelio Enriquec89c4435f60f2fd4fbb07e980e96ebd3Cerezo Cortés, María Ireneb5839d409e66eedcbe5eb325bfbcf78dMICOBAC-UNRodríguez Juan Germán2021-07-29T22:41:59Z2021-07-29T22:41:59Z2021-07-27https://repositorio.unal.edu.co/handle/unal/79870Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, tablasLa tuberculosis (TB) continúa siendo un grave problema de salud pública mundial. En Colombia, el linaje mas prevalente es el Euroamericano. El aumento de la circulación del genotipo Beijing en el territorio nacional, el cual se asocia con resistencia a medicamentos e hipervirulencia causando la muerte de los pacientes, es motivo de investigación. La variante Beijing-Like SIT190 es la más prevalente en Colombia, seguido del Beijing-Clásico SIT1. Los mecanismos patogénicos y modulatorios son desconocidos. El presente trabajo comparó el comportamiento de las cepas 323 (Beijing-Like) y 391 (Beijing-Clásico), en un modelo de TB pulmonar en ratones Balb/c. El curso de la enfermedad fue diferente para cada grupo de animales, los infectados con Beijing-Like tuvieron una mortalidad del 100% al día 45 post-infección (PI), con altas cargas bacilares y neumonía masiva, mientras que el total de los infectados con Beijing-Clásico murieron hacia el día 60 PI por neumonía y necrosis. Se realizó RNA-seq dual para determinar el perfil de expresión génica a los días 3, 14, 28 y 60 PI. En los ratones infectados con Beijing-Clásico se sobreexpresaron genes asociados con respuesta proinflamatoria, comparado con los infectados con Beijing-Like que sobreexpresaron genes asociados con respuesta antiinflamatoria; siendo ambas cepas inductoras de respuesta inmune no protectora. El genotipo Beijing-Like causó enfermedad aguda y fulminante y, Beijing-Clásico causó TB crónica pero severa. A nivel bacteriano, con el RNA-seq de Mtb se logró ~33% del transcriptoma completo a los días 3, 14, 28 y 60 PI. El perfil de expresión fue diferente para 323 Beijing-Like y para 391 Beijing Clásico, evidenciando que las variaciones a nivel regulatorio desencadenan un fenotipo diferente en el hospedador. (Texto tomado de la fuente)Tuberculosis (TB) is a serious global public health problem. In Colombia, the most prevalent lineage is the Euroamerican. The increased circulation of the Beijing genotype which is associated with drug resistance and hypervirulence causing death in patients is intriguing. The Beijing-Like SIT190 variant is the most prevalent in Colombia, followed by the Classical-Beijing SIT1. The pathogenic and modulatory mechanisms triggered by these strains are unknown. In the present work, the course of pulmonary TB was compared in the Balb/c mouse model, in mice infected with Beijing-Like (strain 323) and Classical-Beijing (strain 391). The course of the disease was different for each group of animals, those infected with Beijing-Like had a mortality of 100% by day 45 post-infection (PI), with high bacillary loads and massive pneumonia, and those infected with Classical-Beijing 100% died by day 60 PI from pneumonia and necrosis. Dual RNA-seq was performed to determine the global gene expression profile at days 3, 14, 28 and 60 PI. In mice infected with Classical-Beijing, genes associated with a pro-inflammatory response were overexpressed and in those infected with Beijing-Like, genes associated with an anti-inflammatory response were overexpressed, both strains inducing non-protective immune responses. Beijing-Like caused acute and fulminant disease and Classical-Beijing caused chronic but severe TB. Modulation at the bacterial level was consistent with the type of disease caused in each group of animals, causing the premature death of the animals. At the bacterial level, the RNA-seq of Mtb achieved ~ 33% of the complete transcriptome at days 3, 14, 28 and 60 PI. The expression profile was different for 323 Beijing-Like and for 391 Classic Beijing, showing that variations at the regulatory level trigger a different phenotype in the host. (Text taken from source)DoctoradoDoctora en Ciencias BiomédicasVirulencia de Mycobacterium tuberculosis320 páginasapplication/pdfspaUniversidad Nacional de ColombiaInstituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Ciudad de MéxicoBogotá - Medicina - Doctorado en Ciencias BiomédicasFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::616 - EnfermedadesMycobacterium tuberculosisInmunidadImmunityTuberculosisGenotipo BeijingBalb/cVirulenciaRespuesta InmuneTuberculosisBeijing-GenotypeBalb/cVirulenceImmune ResponseVirulencia, respuesta inmune in vivo y transcriptómica de Mycobacterium tuberculosis genotipo Beijing circulante en ColombiaVirulence, in vivo immune response and transcriptomics of Mycobacterium tuberculosis Beijing genotype circulating in ColombiaTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDColombia1. 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Res Microbiol. 2006;157: 445–455. doi:10.1016/j.resmic.2005.10.007GeneralVIRULENCIA, RESPUESTA INMUNE IN VIVO Y TRANSCRIPTÓMICA DE Mycobacterium tuberculosis GENOTIPO BEIJING CIRCULANTE EN COLOMBIASistema de Información de la Investigación, Extensión y Laboratorios de la Universidad Nacional de Colombia (Hermes), Código del proyecto: 42665Consejo Nacional de Ciencia y Tecnología (CONACyT) -México, Número de contrato: 223279Sistema de Información de la Investigación, Extensión y Laboratorios de la Universidad Nacional de Colombia (Hermes), Código del proyecto: 42665Departamento Administrativo de Ciencia, Tecnología e Innovación (Colciencias-Minciencias), Colombia, Número de contrato: CT-731-2018LICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/79870/1/license.txtcccfe52f796b7c63423298c2d3365fc6MD51ORIGINALMM_Tesis_MICC_01_10_2021 (1).pdfMM_Tesis_MICC_01_10_2021 (1).pdfTesis de Doctorado en Ciencias 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