Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares
La contaminación marina es una gran amenaza para la conservación de los ecosistemas y de su biodiversidad, por lo que se hace necesario realizar estudios enfocados en su recuperación, aplicando tecnologías ambientalmente amigables y de bajo costo como la biorremediación. La biorremediación es el uso...
- Autores:
-
Puentes, Erika Andrea
- Tipo de recurso:
- Fecha de publicación:
- 2024
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/86548
- Palabra clave:
- 500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionados
570 - Biología::579 - Historia natural microorganismos, hongos, algas
600 - Tecnología (Ciencias aplicadas)::607 - Educación, investigación, temas relacionados
Contaminación, hidrocarburos, biorremediación, metabolitos bioactivos, biosurfactantes, tolerancia,
Pollution, hydrocarbons, bioremediation, biosufactant, tolerance, bioactive metabolites
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares |
| dc.title.translated.eng.fl_str_mv |
Effect of crude oil on marine microalgae, about heterotrophic growth and the production of extracellular metabolites n of extracellular metabolites |
| title |
Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares |
| spellingShingle |
Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares 500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionados 570 - Biología::579 - Historia natural microorganismos, hongos, algas 600 - Tecnología (Ciencias aplicadas)::607 - Educación, investigación, temas relacionados Contaminación, hidrocarburos, biorremediación, metabolitos bioactivos, biosurfactantes, tolerancia, Pollution, hydrocarbons, bioremediation, biosufactant, tolerance, bioactive metabolites |
| title_short |
Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares |
| title_full |
Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares |
| title_fullStr |
Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares |
| title_full_unstemmed |
Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares |
| title_sort |
Efecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelulares |
| dc.creator.fl_str_mv |
Puentes, Erika Andrea |
| dc.contributor.advisor.none.fl_str_mv |
Blandón, Lina Campos Campos, Néstor Hernando Patiño, Albert |
| dc.contributor.author.none.fl_str_mv |
Puentes, Erika Andrea |
| dc.contributor.hostingInstitution.none.fl_str_mv |
Invemar - Instituto de Investigaciones marinas y costeras |
| dc.contributor.researchgroup.spa.fl_str_mv |
Fauna Marina Colombiana: Biodiversidad y Usos |
| dc.subject.ddc.spa.fl_str_mv |
500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionados 570 - Biología::579 - Historia natural microorganismos, hongos, algas 600 - Tecnología (Ciencias aplicadas)::607 - Educación, investigación, temas relacionados |
| topic |
500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionados 570 - Biología::579 - Historia natural microorganismos, hongos, algas 600 - Tecnología (Ciencias aplicadas)::607 - Educación, investigación, temas relacionados Contaminación, hidrocarburos, biorremediación, metabolitos bioactivos, biosurfactantes, tolerancia, Pollution, hydrocarbons, bioremediation, biosufactant, tolerance, bioactive metabolites |
| dc.subject.proposal.none.fl_str_mv |
Contaminación, hidrocarburos, biorremediación, metabolitos bioactivos, biosurfactantes, tolerancia, Pollution, hydrocarbons, bioremediation, biosufactant, tolerance, bioactive metabolites |
| description |
La contaminación marina es una gran amenaza para la conservación de los ecosistemas y de su biodiversidad, por lo que se hace necesario realizar estudios enfocados en su recuperación, aplicando tecnologías ambientalmente amigables y de bajo costo como la biorremediación. La biorremediación es el uso de organismos, sus células, partes o metabolitos, para recuperar un ambiente contaminado a través de la transformación o degradación de compuestos tóxicos recalcitrantes como los hidrocarburos, en una forma menos tóxica o no tóxica. En este sentido, el uso de microalgas y/o sus componentes, es una alternativa prometedora ya que presentan tolerancia a factores de estrés como los ocasionados por la presencia de petróleo en el entorno, evidenciando la producción de metabolitos como biosurfactantes participantes de la actividad biorremediadora. Este proyecto tuvo como objetivo principal evaluar la respuesta metabólica a la presencia del petróleo, a través de la detección de biosurfactantes extracelulares de microalgas marinas aisladas del Mar Caribe colombiano, en condiciones de luz y oscuridad, empleando herramientas metabolómicas. Para ello, se realizó un screening de tolerancia celular con petróleo en suspensión al 1 %. Se evidencio la producción de un extracto extracelular rico en biosurfactantes, por Nannochloris sp., la cual al ser incubada en oscuridad produjo un extracto con mayor presencia de estos compuestos. Los resultados obtenidos, aportan información científica que contribuye a mejorar el conocimiento del proceso de tolerancia al petróleo que exhiben algunas microalgas marinas (Texto tomado de la fuente) |
| publishDate |
2024 |
| dc.date.accessioned.none.fl_str_mv |
2024-07-17T21:55:09Z |
| dc.date.available.none.fl_str_mv |
2024-07-17T21:55:09Z |
| dc.date.issued.none.fl_str_mv |
2024-07-16 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
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info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/acceptedVersion |
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Text |
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http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/86548 |
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Universidad Nacional de Colombia |
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Repositorio Institucional Universidad Nacional de Colombia |
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https://repositorio.unal.edu.co/ |
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https://repositorio.unal.edu.co/handle/unal/86548 https://repositorio.unal.edu.co/ |
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Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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The production of extracellular carbohydrates by estuarine benthic diatoms: The effects of growth phase and light and dark treatment. Journal of Phycology, 36(2), 321–333. https://doi.org/10.1046/j.1529-8817.2000.99148.x Somogyi, B., Felföldi, T., Solymosi, K., Flieger, K., Márialigeti, K., Böddi, B., & Vörös, L. (2013). One step closer to eliminating the nomenclatural problems of minute coccoid green algae: Pseudochloris wilhelmii, gen. et sp. nov. (Trebouxiophyceae, Chlorophyta). European Journal of Phycology, 48(4), 427–436. https://doi.org/10.1080/09670262.2013.854411 Subashchandrabose, S. R., Krishnan, K., Gratton, E., Megharaj, M., & Naidu, R. (2014). Potential of fluorescence imaging techniques to monitor mutagenic PAH uptake by microalga. Environmental Science and Technology, 48(16), 9152–9160. https://doi.org/10.1021/es500387v Subramanian, G., Yadav, G., & Sen, R. (2016). 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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Blandón, Lina15654a57b58cfd2fb21e73b08224c039Campos Campos, Néstor Hernando07b6b37cec1e87c71c366847a7ecae7b600Patiño, Albertef83c78dfe98303f7b54a35f5526a1e1600Puentes, Erika Andreab94e314e5c6d962f6c2f9d75dafcf4cfInvemar - Instituto de Investigaciones marinas y costerasFauna Marina Colombiana: Biodiversidad y Usos2024-07-17T21:55:09Z2024-07-17T21:55:09Z2024-07-16https://repositorio.unal.edu.co/handle/unal/86548Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/La contaminación marina es una gran amenaza para la conservación de los ecosistemas y de su biodiversidad, por lo que se hace necesario realizar estudios enfocados en su recuperación, aplicando tecnologías ambientalmente amigables y de bajo costo como la biorremediación. La biorremediación es el uso de organismos, sus células, partes o metabolitos, para recuperar un ambiente contaminado a través de la transformación o degradación de compuestos tóxicos recalcitrantes como los hidrocarburos, en una forma menos tóxica o no tóxica. En este sentido, el uso de microalgas y/o sus componentes, es una alternativa prometedora ya que presentan tolerancia a factores de estrés como los ocasionados por la presencia de petróleo en el entorno, evidenciando la producción de metabolitos como biosurfactantes participantes de la actividad biorremediadora. Este proyecto tuvo como objetivo principal evaluar la respuesta metabólica a la presencia del petróleo, a través de la detección de biosurfactantes extracelulares de microalgas marinas aisladas del Mar Caribe colombiano, en condiciones de luz y oscuridad, empleando herramientas metabolómicas. Para ello, se realizó un screening de tolerancia celular con petróleo en suspensión al 1 %. Se evidencio la producción de un extracto extracelular rico en biosurfactantes, por Nannochloris sp., la cual al ser incubada en oscuridad produjo un extracto con mayor presencia de estos compuestos. Los resultados obtenidos, aportan información científica que contribuye a mejorar el conocimiento del proceso de tolerancia al petróleo que exhiben algunas microalgas marinas (Texto tomado de la fuente)Marine pollution is a significant threat to ecosystem conservation and its biodiversity. Therefore, it is necessary to carry out studies focused on ecosystem recovery, applying environmentally friendly and low-cost technologies such as bioremediation. Bioremediation is the use of organisms, their cells, parts or metabolites, to remediate a contaminated environment through the transformation or degradation of recalcitrant toxic compounds such as crude oil into a less toxic or non-toxic form. In this sense, using microalgae and /or its components is a promising alternative. Microalgae can be tolerant to stress factors such as oil crude, in response, they can produce metabolites like biosurfactants that participates in the bioremediation activity. The main objective of this project was to evaluate the metabolic response of microalgae isolated from the Colombian Caribbean, exposed to the presence of crude oil, as well as the detection of extracellular biosurfactants, under light and dark conditions, using metabolomic tools. A screening with crude oil in suspension at 1% was carried out, achieving evidence of the production of extracellular extract rich in biosurfactants by a strain Nannochloris sp. The same strain cultured without light, produced this extract in greater quantity. The results provide scientific information that improves the knowledge of the tolerance process to crude oil that some marine microalgae exhibit.MaestríaMagíster en Ciencias - BiologíaComparación de la producción de metabolitos extracelulares entre tratamientos: 1. Tratamiento de exposición a pétroleo: Incubados en luz y oscuridad 2. Grupos control, control abiotico de medio de cultivo con petróleo y control de crecimiento de las microalgas en medio de cultivoBioprospección marinaOtra. Sede Caribe100 paginasapplication/pdfspaUniversidad Nacional sede CaribeCaribe - Caribe - Maestría en Ciencias - BiologíaCentro de estudios en Ciencias del mar-CECIMARFacultad de CienciasSan Andrés IslasUniversidad Nacional de Colombia - Sede Caribe500 - Ciencias naturales y matemáticas::507 - Educación, investigación, temas relacionados570 - Biología::579 - Historia natural microorganismos, hongos, algas600 - Tecnología (Ciencias aplicadas)::607 - Educación, investigación, temas relacionadosContaminación, hidrocarburos, biorremediación, metabolitos bioactivos, biosurfactantes, tolerancia,Pollution, hydrocarbons, bioremediation, biosufactant, tolerance, bioactive metabolitesEfecto del petróleo en microalgas marinas, sobre el crecimiento heterotrófico y la producción de metabolitos extracelularesEffect of crude oil on marine microalgae, about heterotrophic growth and the production of extracellular metabolites n of extracellular metabolitesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAlmaliti, J., Malloy, K. 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Potencial biotecnológico para la producción sustentable de biodisel de microalgas oleaginosas aisladas del Río Itaya, Loreto, Perú. Ecología Aplicada, 13(1–2), 169. https://doi.org/10.21704/rea.v13i1-2.467Cocci, P., Mosconi, G., Bracchetti, L., Nalocca, J. M., Frapiccini, E., Marini, M., Caprioli, G., Sagratini, G., & Palermo, F. A. (2018). Investigating the potential impact of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) on gene biomarker expression and global DNA methylation in loggerhead sea turtles (Caretta caretta) from the Adriatic Sea. Science of the Total Environment, 619–620, 49–57. https://doi.org/10.1016/j.scitotenv.2017.11.118Collins, L., Alvarez, D., & Chauhan, A. (2014). Phycoremediation Coupled with Generation of Value-Added Products. In Microbial Biodegradation and Bioremediation (pp. 342–387). Elsevier Inc. https://doi.org/10.1016/B978-0-12-800021-2.00015-7Darienko, T., & Pröschold, T. (2015). 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Organisms Diversity and Evolution, 11(3), 173–192. https://doi.org/10.1007/s13127-011-0050-6EstudiantesLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86548/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1010186171.2022.pdf1010186171.2022.pdfTesis de maestría en Ciencias Biologíaapplication/pdf2995666https://repositorio.unal.edu.co/bitstream/unal/86548/2/1010186171.2022.pdf21a22bb27b663a66f08e9352e5b408c5MD52THUMBNAIL1010186171.2022.pdf.jpg1010186171.2022.pdf.jpgGenerated Thumbnailimage/jpeg4511https://repositorio.unal.edu.co/bitstream/unal/86548/3/1010186171.2022.pdf.jpg73363ff99b2786df923e05b880d8be3aMD53unal/86548oai:repositorio.unal.edu.co:unal/865482024-07-17 23:05:55.959Repositorio Institucional Universidad Nacional de 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