Expresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celular
ilustraciones, gráficas, tablas
- Autores:
-
Muñoz Roa, Esther Natalia
- Tipo de recurso:
- Fecha de publicación:
- 2020
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/80286
- Palabra clave:
- 610 - Medicina y salud::612 - Fisiología humana
610 - Medicina y salud::614 - Medicina Forense; incidencia de lesiones, heridas, enfermedades; medicina preventiva pública
Cytology
Citología
Melanoma
5-Bromo-2´-deoxiuridina
Invasión
Migración
Proliferación
miRNAs
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Expresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celular |
| dc.title.translated.eng.fl_str_mv |
Expression of ROCK 2 kinase and microRNAs-138-5p and 455-3p in B16 melanoma cells exposed to 5-Bromo-2´-deoxyuridine and their association with cell proliferation, adhesion, migration and viability |
| title |
Expresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celular |
| spellingShingle |
Expresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celular 610 - Medicina y salud::612 - Fisiología humana 610 - Medicina y salud::614 - Medicina Forense; incidencia de lesiones, heridas, enfermedades; medicina preventiva pública Cytology Citología Melanoma 5-Bromo-2´-deoxiuridina Invasión Migración Proliferación miRNAs |
| title_short |
Expresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celular |
| title_full |
Expresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celular |
| title_fullStr |
Expresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celular |
| title_full_unstemmed |
Expresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celular |
| title_sort |
Expresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celular |
| dc.creator.fl_str_mv |
Muñoz Roa, Esther Natalia |
| dc.contributor.advisor.none.fl_str_mv |
Gómez Grosso, Luis Alberto |
| dc.contributor.author.none.fl_str_mv |
Muñoz Roa, Esther Natalia |
| dc.contributor.researchgroup.spa.fl_str_mv |
Fisiología Molecular |
| dc.subject.ddc.spa.fl_str_mv |
610 - Medicina y salud::612 - Fisiología humana 610 - Medicina y salud::614 - Medicina Forense; incidencia de lesiones, heridas, enfermedades; medicina preventiva pública |
| topic |
610 - Medicina y salud::612 - Fisiología humana 610 - Medicina y salud::614 - Medicina Forense; incidencia de lesiones, heridas, enfermedades; medicina preventiva pública Cytology Citología Melanoma 5-Bromo-2´-deoxiuridina Invasión Migración Proliferación miRNAs |
| dc.subject.mesh.eng.fl_str_mv |
Cytology |
| dc.subject.mesh.spa.fl_str_mv |
Citología |
| dc.subject.proposal.spa.fl_str_mv |
Melanoma 5-Bromo-2´-deoxiuridina Invasión Migración Proliferación |
| dc.subject.proposal.eng.fl_str_mv |
miRNAs |
| description |
ilustraciones, gráficas, tablas |
| publishDate |
2020 |
| dc.date.issued.none.fl_str_mv |
2020 |
| dc.date.accessioned.none.fl_str_mv |
2021-09-24T02:09:16Z |
| dc.date.available.none.fl_str_mv |
2021-09-24T02:09:16Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/80286 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/80286 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Goto, Hidemasa, Hidetaka Kosako, Kazushi Tanabe, Maki Yanagida, Minoru Sakurai, Mutsuki Amano, Kozo Kaibuchi, and Masaki Inagaki. 1998. “Phosphorylation of Vimentin by Rho-Associated Kinase at a Unique Amino-Terminal Site That Is Specifically Phosphorylated during Cytokinesis.” Journal of Biological Chemistry 273 (19): 11728–36. https://doi.org/10.1074/jbc.273.19.11728. Gray-Schopfer, Vanessa, Claudia Wellbrock, and Richard Marais. 2007. “Melanoma Biology and New Targeted Therapy.” Nature 445 (February): 851. http://dx.doi.org/10.1038/nature05661. Gunturiz, María Luz., and Luis Alberto. Gómez. 2011. “Expresión de Los Genes Que Regulan La Síntesis de Melanina: MITF-M, TRP1 y TRP2 En Células de Melanoma Maligno B16 y A375.” In Instituto Nacional de Salud, 51. Bogotá: Biomédica. 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xv, 74 páginas |
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Universidad Nacional de Colombia |
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Bogotá - Medicina - Maestría en Bioquímica |
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Departamento de Ciencias Fisiológicas |
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Facultad de Medicina |
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Bogotá, Colombia |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gómez Grosso, Luis Alberto4e7823be0e6e7a95d83cee153f1a7644Muñoz Roa, Esther Natalia7ce4cdbc9b40783cd112959632e3c5ebFisiología Molecular2021-09-24T02:09:16Z2021-09-24T02:09:16Z2020https://repositorio.unal.edu.co/handle/unal/80286Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, gráficas, tablasROCK 2 participa en la reestructuración del citoesqueleto, adhesión y contractilidad celular; aunque se conoce su asociación con la proliferación, migración e invasión en diferentes modelos celulares, poco se sabe sobre las bases moleculares de su regulación en células de melanoma. El objetivo de este trabajo fue evaluar los cambios en la morfología, proliferación y las variaciones de expresión de ROCK 2, miR-138-5p y miR-455-3p, dianas moleculares de ROCK 2, en células B16F1 expuestas al análogo de timidina, 5- Bromo-2'- deoxiuridina (BrdU). La exposición a BrdU (72 h) indujo una disminución del 65% en la proliferación, y un fenotipo senescente con aumento de 2,2 veces en el área celular aparente, aumento de 1,27 veces en el diámetro y la granulación. Las células expuestas mejoraron su capacidad de adhesión, circularidad y cierre de heridas y disminuyeron su migración en la cámara de Boyden en un 86,6%. La expresión de la proteína ROCK 2 mostró una disminución de aproximada de 3 veces en su mRNA, y después de la adición de un inhibidor de su actividad (Y27632), se observaron variaciones en la movilidad, polimerización de F-actina y VIM. Estos resultados coincidieron con una disminución de la expresión de miR-138-5p (1,8 veces) y un aumento de miR-455-3p (2,39 veces). En general, es posible sugerir que ROCK 2 participa en la regulación de los cambios morfológicos observados después de la exposición a BrdU, y su acción podría estar regulada, al menos en parte, por la expresión de miR-138-5p y miR-455-3p. (texto tomado de la fuente)ROCK 2 is involved in cytoskeleton restructuring, cell adhesion and contractility; although its association with proliferation, migration and invasion is known in different cell models, little is known about the molecular basis of its regulation in melanoma cells. The aim of this work was to evaluate changes in morphology, proliferation and expression variations of ROCK 2, miR-138-5p and miR-455-3p, molecular targets of ROCK 2, in B16F1 cells exposed to the thymidine analog, 5- Bromo-2'- deoxyuridine (BrdU). Exposure to BrdU (72 h) induced a 65% decrease in proliferation, and a senescent phenotype with 2.2-fold increase in apparent cell area, 1.27-fold increase in diameter and granulation. Exposed cells improved their adhesion, circularity and wound closure capacity and decreased their migration in the Boyden chamber by 86.6%. ROCK 2 protein expression showed an approximately 3-fold decrease in its mRNA, and after addition of an inhibitor of its activity (Y27632), variations in motility, F-actin polymerization and VIM were observed. These results coincided with a decrease in miR-138-5p expression (1.8-fold) and an increase in miR-455-3p (2.39-fold). Overall, it is possible to suggest that ROCK 2 participates in the regulation of the morphological changes observed after BrdU exposure, and its action could be regulated, at least in part, by the expression of miR-138-5p and miR-455 -3p.Instituto Nacional de SaludMaestríaMagíster en BioquímicaBases moleculares de la diferenciación tumoral y transformación malignaxv, 74 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina - Maestría en BioquímicaDepartamento de Ciencias FisiológicasFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::612 - Fisiología humana610 - Medicina y salud::614 - Medicina Forense; incidencia de lesiones, heridas, enfermedades; medicina preventiva públicaCytologyCitologíaMelanoma5-Bromo-2´-deoxiuridinaInvasiónMigraciónProliferaciónmiRNAsExpresión de la Cinasa ROCK 2 y de los microRNAs-138-5p y 455-3p en células de melanoma B16 expuestas a 5-Bromo-2´-deoxiuridina y su asociación con proliferación, adhesión, migración y viabilidad celularExpression of ROCK 2 kinase and microRNAs-138-5p and 455-3p in B16 melanoma cells exposed to 5-Bromo-2´-deoxyuridine and their association with cell proliferation, adhesion, migration and viabilityTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbbott, J, and H Holtzer. 1968. “The Loss of Phenotypic Traits by Differentiated Cells, V. 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