Estudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizados

En la presente investigación se realizó un estudio de la interacción de nanopartículas de oro y plata con DNA genómico por medio de la Espectroscopia Raman. Se realizaron tres ciclos de síntesis de nanopartículas de oro y plata por medio del método de radiación por microondas, las nanopartículas se...

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Autores:
Báez Cruz, Ricardo Eulises
Tipo de recurso:
Fecha de publicación:
2015
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
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oai:repositorio.unal.edu.co:unal/55774
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https://repositorio.unal.edu.co/handle/unal/55774
http://bdigital.unal.edu.co/51237/
Palabra clave:
5 Ciencias naturales y matemáticas / Science
53 Física / Physics
Nanopartículas oro y plata
Raman
DNA
Nanoparticles Au y Ag
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openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_7bef4b923b284537f01cb027ad2f8bd7
oai_identifier_str oai:repositorio.unal.edu.co:unal/55774
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Estudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizados
dc.title.translated.eng.fl_str_mv SERS spectroscopic study of genomic DNA interacting with Au and Ag nanoparticles through localized plasmons
title Estudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizados
spellingShingle Estudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizados
5 Ciencias naturales y matemáticas / Science
53 Física / Physics
Nanopartículas oro y plata
Raman
DNA
Nanoparticles Au y Ag
title_short Estudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizados
title_full Estudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizados
title_fullStr Estudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizados
title_full_unstemmed Estudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizados
title_sort Estudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizados
dc.creator.fl_str_mv Báez Cruz, Ricardo Eulises
dc.contributor.author.spa.fl_str_mv Báez Cruz, Ricardo Eulises
dc.subject.ddc.spa.fl_str_mv 5 Ciencias naturales y matemáticas / Science
53 Física / Physics
topic 5 Ciencias naturales y matemáticas / Science
53 Física / Physics
Nanopartículas oro y plata
Raman
DNA
Nanoparticles Au y Ag
dc.subject.proposal.spa.fl_str_mv Nanopartículas oro y plata
Raman
DNA
Nanoparticles Au y Ag
description En la presente investigación se realizó un estudio de la interacción de nanopartículas de oro y plata con DNA genómico por medio de la Espectroscopia Raman. Se realizaron tres ciclos de síntesis de nanopartículas de oro y plata por medio del método de radiación por microondas, las nanopartículas se caracterizaron usando microscopia electrónica de trasmisión (TEM) y absorción óptica (Uv-Visible). El DNA se extrajo del bazo de cerdo y se caracterizó por absorción óptica (Uv-Visible. Se encontró que las partículas sintetizadas estabilizaban en nanoesferas y adquieran un plasmon entre los 320 y 420 nm. Del DNA se encontró que estaba desproteinizado y con un PH estable. Las dos muestras fueron conjugas de dos formas, en la primera se combinaron en solución variando los ml de nanopartículas (oro y plata) y se realizaron las mediciones Raman entre 200 y 4000 cm−1. La segunda muestra se estudió en fibra (solido) agregando ml de nanopartículas (oro y plata) en un portaobjeto con la finalidad de secar las muestras a estos sustratos se les realizo igualmente pruebas de espectroscopia Raman 200 y 4000 cm−1. Se encontró que las NPs en solución ancladas al DNA no amplificaban la señal Raman del DNA debido al apantallamiento del citrato estabilizante envolate de las nanopartículas de oro. En contraposición, las nanopartículas de plata en solución amplificaron la señal Raman de la beses nitrógenos de DNA. Respecto a las muestras solidas tantas las nanopartículas de oro y plata realizaron amplificación del %100 de las bases nitrogenadas de DNA (Texto tomado de la fuente)
publishDate 2015
dc.date.issued.spa.fl_str_mv 2015
dc.date.accessioned.spa.fl_str_mv 2019-07-02T11:28:05Z
dc.date.available.spa.fl_str_mv 2019-07-02T11:28:05Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/55774
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/51237/
url https://repositorio.unal.edu.co/handle/unal/55774
http://bdigital.unal.edu.co/51237/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Sede Manizales Facultad de Ciencias Exactas y Naturales Departamento de Física y Química
Departamento de Física y Química
dc.relation.references.spa.fl_str_mv La cromatina durante el ciclo celular.
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Barrio, Justo V.: Sintesis de nanoparticulas de oro y plata y estudio de su deposicion sobre nanoestructuras de base silicea. (2012), p. 2
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Solorzano, Daniel M.: Determinacion de tamanos de nanoparticulas metalicas mediante tecnicas espectroscopicas y polarimetricas. (2012), p. 13–18
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dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Báez Cruz, Ricardo Eulisesb4a15e89-52ca-4210-ac4f-675e6795a38b3002019-07-02T11:28:05Z2019-07-02T11:28:05Z2015https://repositorio.unal.edu.co/handle/unal/55774http://bdigital.unal.edu.co/51237/En la presente investigación se realizó un estudio de la interacción de nanopartículas de oro y plata con DNA genómico por medio de la Espectroscopia Raman. Se realizaron tres ciclos de síntesis de nanopartículas de oro y plata por medio del método de radiación por microondas, las nanopartículas se caracterizaron usando microscopia electrónica de trasmisión (TEM) y absorción óptica (Uv-Visible). El DNA se extrajo del bazo de cerdo y se caracterizó por absorción óptica (Uv-Visible. Se encontró que las partículas sintetizadas estabilizaban en nanoesferas y adquieran un plasmon entre los 320 y 420 nm. Del DNA se encontró que estaba desproteinizado y con un PH estable. Las dos muestras fueron conjugas de dos formas, en la primera se combinaron en solución variando los ml de nanopartículas (oro y plata) y se realizaron las mediciones Raman entre 200 y 4000 cm−1. La segunda muestra se estudió en fibra (solido) agregando ml de nanopartículas (oro y plata) en un portaobjeto con la finalidad de secar las muestras a estos sustratos se les realizo igualmente pruebas de espectroscopia Raman 200 y 4000 cm−1. Se encontró que las NPs en solución ancladas al DNA no amplificaban la señal Raman del DNA debido al apantallamiento del citrato estabilizante envolate de las nanopartículas de oro. En contraposición, las nanopartículas de plata en solución amplificaron la señal Raman de la beses nitrógenos de DNA. Respecto a las muestras solidas tantas las nanopartículas de oro y plata realizaron amplificación del %100 de las bases nitrogenadas de DNA (Texto tomado de la fuente)Abstract : In this research a study of the interaction of nanoparticles of gold and silver with genomic DNA by means of Raman spectroscopy was performed. Three cycles of synthesis of nanoparticles of gold and silver by the method of microwave radiation were performed, the nanoparticles were characterized using transmission electron microscopy (TEM) and optical absorption (UV-visible). The DNA was extracted from pig spleen and was characterized by optical absorption (UV-Vis. It was found that the synthesized particles stabilized in nanospheres and purchase a plasmon between 320 and 420 nm. The DNA was found to be deproteinized and a PH stable. The two samples were conjugas in two ways, in the first solution were combined in varying ml nanoparticles (gold and silver) and Raman measurements between 200 and 4000 cm−1 were performed. The second sample was studied fiber (solid) was added ml of nanoparticles (gold and silver) on a slide with the aim of drying the samples at these substrates were subjected to Raman spectroscopy testing igualemnte 200 and 4000 cm−1. It was found that NPs DNA in solution anchored not amplify the Raman signal due to shielding DNA citrate envolate stabilizing the gold nanoparticles. In contrast, the silver nanoparticles in solution Raman amplified signal DNA nitrogenous base. Regarding the many samples solid gold nanoparticles and silver amplification performed % 100 of the nitrogenous bases of DNAMaestríaapplication/pdfspaUniversidad Nacional de Colombia Sede Manizales Facultad de Ciencias Exactas y Naturales Departamento de Física y QuímicaDepartamento de Física y QuímicaLa cromatina durante el ciclo celular.A Khan, S Alrokayan M Alsalhi M Alhoshan A S.:B, Angela S. C.: ACOPLAMIENTO DE PLASMONES LOCALIZADOS EN NANOSISTEMAS. 56 (2) (2010), p. 147–154B, Angela S. C.: ACOPLAMIENTO DE PLASMONES LOCALIZADOS EN NANOSISTEMAS. XXXVI (2012), p. 138Barrio, Justo V.: Sintesis de nanoparticulas de oro y plata y estudio de su deposicion sobre nanoestructuras de base silicea. (2012), p. 2FERREIRA, SZPINIAC Beatriz; GRASSI E.: Manual de Genetica.Gonzalez, Edgar E.: La nueva era de los nanomateriales. 1 (2013), p. 35–45Luis, José: ADN: Estructura molecular.N, Raul: Biologia Moleculas Antes y despues de la doble helice.O, Nestor: ADN:una molecula maravillosa.y Santiago Sanchez-Cortes, Jose Vicente Garcia-Ramos: Espectroscopía vibracional sobre nanoestructuras metálicas (SERS y SEIR): nuevos sustratos y aplicaciones. 39 (2) (2006), p. 147–154Santillan, Jesica Maria J.: Estudios de las propiedades opticas de materiales nanoestructurados y aplicaciones. (2013), p. 11–24Sinencio., V Altuzar. C Mendoza Barrera. ML Munoz. JG Mendoza Alvarezy. F S.: Analisis cuantitativo de interacciones moleculares proteina proteina mediante la combinacion de microarreglos y un lector optico basado en el fenomeno de resonancia de plasmones superficiales. 36 (138) (2012), p. 15–24Solorzano, Daniel M.: Determinacion de tamanos de nanoparticulas metalicas mediante tecnicas espectroscopicas y polarimetricas. (2012), p. 13–18Tognalli, Nicolas G.: Nanoestructuras metalicas para espectroscopia SERS de sistemas biomimeticos y de sensado. (2008), p. 48A. Serrano, V. Collado J. Rubio-Zuazo C. Monton G. ; García, M. A.: Simultaneous Surface Plasmon Resonance and X-ray Absorption Spectroscopy.Angshuman Pal, Surekha D.: Microwave-assisted synthesis of silver nanoparticles using ethanol as a reducing agent. En: Materials Chemistry and Physics 114 2009() (2009), p. 530–532Anju K. Augustine, M. 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(2011), p. 225 Ciencias naturales y matemáticas / Science53 Física / PhysicsNanopartículas oro y plataRamanDNANanoparticles Au y AgEstudio por espectroscopia SERS de ADN genómico en interacción con nanoparticulas de Au y Ag a través de plasmones localizadosSERS spectroscopic study of genomic DNA interacting with Au and Ag nanoparticles through localized plasmonsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMORIGINAL1013589528.2015.pdf1013589528.2015.pdfTesis de Maestría en Ciencias - Físicaapplication/pdf36556833https://repositorio.unal.edu.co/bitstream/unal/55774/1/1013589528.2015.pdf4c973cf9d3bfdaaecca2cf12b96c37a4MD51U.FT.09.006.004 Licencia y autorización para publicación de obras en el repositorio institucional UN v4 (1).pdfU.FT.09.006.004 Licencia y autorización para publicación de obras en el repositorio institucional UN v4 (1).pdfapplication/pdf221730https://repositorio.unal.edu.co/bitstream/unal/55774/2/U.FT.09.006.004%20Licencia%20y%20autorizaci%c3%b3n%20para%20publicaci%c3%b3n%20de%20obras%20en%20el%20repositorio%20institucional%20UN%20v4%20%281%29.pdf10dd8ebe1c88bb5fdca3698b9eac5d9bMD52THUMBNAIL1013589528.2015.pdf.jpg1013589528.2015.pdf.jpgGenerated Thumbnailimage/jpeg5519https://repositorio.unal.edu.co/bitstream/unal/55774/3/1013589528.2015.pdf.jpg9492742bb9dedb0753572b0b0cb036f2MD53U.FT.09.006.004 Licencia y autorización para publicación de obras en el repositorio institucional UN v4 (1).pdf.jpgU.FT.09.006.004 Licencia y autorización para publicación de obras en el repositorio institucional UN v4 (1).pdf.jpgGenerated Thumbnailimage/jpeg5750https://repositorio.unal.edu.co/bitstream/unal/55774/4/U.FT.09.006.004%20Licencia%20y%20autorizaci%c3%b3n%20para%20publicaci%c3%b3n%20de%20obras%20en%20el%20repositorio%20institucional%20UN%20v4%20%281%29.pdf.jpgd3fb75bf6fa9f6d1bce44d5e057842f4MD54unal/55774oai:repositorio.unal.edu.co:unal/557742023-03-14 23:08:58.701Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

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