Evaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celulares
ilustraciones, diagramas, tablas
- Autores:
-
Londoño Berrio, Maritza
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/82250
- Palabra clave:
- 660 - Ingeniería química
Citotoxicidad por mediación celular
Cell-mediated cytotoxicity
Toxicoproteómica
Nanopartículas
Diésel con mezcla de alcoholes
Toxicoproteomics
Nanoparticles
Diesel with a mixture of alcohols
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Evaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celulares |
| dc.title.translated.eng.fl_str_mv |
In vitro evaluation of the effect of particulate matter and a potential treatment on two cell lines |
| title |
Evaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celulares |
| spellingShingle |
Evaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celulares 660 - Ingeniería química Citotoxicidad por mediación celular Cell-mediated cytotoxicity Toxicoproteómica Nanopartículas Diésel con mezcla de alcoholes Toxicoproteomics Nanoparticles Diesel with a mixture of alcohols |
| title_short |
Evaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celulares |
| title_full |
Evaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celulares |
| title_fullStr |
Evaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celulares |
| title_full_unstemmed |
Evaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celulares |
| title_sort |
Evaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celulares |
| dc.creator.fl_str_mv |
Londoño Berrio, Maritza |
| dc.contributor.advisor.none.fl_str_mv |
Ortiz Trujillo, Isabel Cristina López, Juan Bautista |
| dc.contributor.author.none.fl_str_mv |
Londoño Berrio, Maritza |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Bioinformática y Biología de Sistemas |
| dc.subject.ddc.spa.fl_str_mv |
660 - Ingeniería química |
| topic |
660 - Ingeniería química Citotoxicidad por mediación celular Cell-mediated cytotoxicity Toxicoproteómica Nanopartículas Diésel con mezcla de alcoholes Toxicoproteomics Nanoparticles Diesel with a mixture of alcohols |
| dc.subject.lemb.none.fl_str_mv |
Citotoxicidad por mediación celular Cell-mediated cytotoxicity |
| dc.subject.proposal.spa.fl_str_mv |
Toxicoproteómica Nanopartículas Diésel con mezcla de alcoholes |
| dc.subject.proposal.eng.fl_str_mv |
Toxicoproteomics Nanoparticles Diesel with a mixture of alcohols |
| description |
ilustraciones, diagramas, tablas |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-09-05T15:57:17Z |
| dc.date.available.none.fl_str_mv |
2022-09-05T15:57:17Z |
| dc.date.issued.none.fl_str_mv |
2022 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/acceptedVersion |
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Text |
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http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/82250 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/82250 https://repositorio.unal.edu.co/ |
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Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
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Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544670/ Xiong S, George S, Yu H, Damoiseaux R, France B, Ng KW, et al. Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles. Arch Toxicol. junio de 2013;87(6):1075-86. Doak SH, Manshian B, Jenkins GJS, Singh N. In vitro genotoxicity testing strategy for nanomaterials and the adaptation of current OECD guidelines. Mutat Res. 14 de junio de 2012;745(1-2):104-11. Henderson L, Wolfreys A, Fedyk J, Bourner C, Windebank S. The ability of the Comet assay to discriminate between genotoxins and cytotoxins. Mutagenesis. enero de 1998;13(1):89-94. Zivkovic L, Akar B, Roux BM, Spremo Potparevic B, Bajic V, Brey EM. Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. J Biomed Mater Res A. 2017;105(1):284-91. Magdolenova Z, Collins A, Kumar A, Dhawan A, Stone V, Dusinska M. Mechanisms of genotoxicity. A review of in vitro and in vivo studies with engineered nanoparticles. Nanotoxicology. mayo de 2014;8(3):233-78. Kisin ER, Murray AR, Keane MJ, Shi X-C, Schwegler-Berry D, Gorelik O, et al. Single-walled Carbon Nanotubes: Geno- and Cytotoxic Effects in Lung Fibroblast V79 Cells. Journal of Toxicology and Environmental Health, Part A. 13 de noviembre de 2007;70(24):2071-9. Li Y, Chen DH, Yan J, Chen Y, Mittelstaedt RA, Zhang Y, et al. Genotoxicity of silver nanoparticles evaluated using the Ames test and in vitro micronucleus assay. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 14 de junio de 2012;745(1):4-10. The photogenotoxicity of titanium dioxide particles - ScienceDirect [Internet]. [citado 24 de noviembre de 2021]. Disponible en: https://www.sciencedirect.com/science/article/pii/S1383571897001265?casa_token=Rh-WCuPH8JUAAAAA:QYQBvCrRb2Ideeq6HKGmtowKsEnM_iorfwddX0mNGEQkT_8nexF309TRWo9kKU-Xs8qH4g4sBg Dusinska M, Tulinska J, El Yamani N, Kuricova M, Liskova A, Rollerova E, et al. Immunotoxicity, genotoxicity and epigenetic toxicity of nanomaterials: New strategies for toxicity testing? Food Chem Toxicol. noviembre de 2017;109(Pt 1):797-811 Sawai J, Kojima H, Kano F, Igarashi H, Hashimoto A, Kawada E, et al. Short Communication: Ames assay with Salmonella typhimurium TA102 for mutagenicity and antimutagenicity of metallic oxide powders having antibacterial activities. World Journal of Microbiology and Biotechnology. 1 de octubre de 1998;14:773-5. Fu PP, Xia Q, Hwang H-M, Ray PC, Yu H. Mechanisms of nanotoxicity: Generation of reactive oxygen species. Journal of Food and Drug Analysis. 1 de marzo de 2014;22(1):64-75. Choudhury SR, Ordaz J, Lo C-L, Damayanti NP, Zhou F, Irudayaraj J. From the Cover: Zinc oxide Nanoparticles-Induced Reactive Oxygen Species Promotes Multimodal Cyto- and Epigenetic Toxicity. Toxicol Sci. 01 de 2017;156(1):261-74. |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ortiz Trujillo, Isabel Cristinaddffc2883d89562ffa5884e6f6763a78López, Juan Bautista48cddd926e9153b1685dca90131e1c4eLondoño Berrio, Maritza99072322951ca7f7299399cb0d3e177fGrupo de Investigación en Bioinformática y Biología de Sistemas2022-09-05T15:57:17Z2022-09-05T15:57:17Z2022https://repositorio.unal.edu.co/handle/unal/82250Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, tablasLa interacción de los sistemas biológicos con agentes externos puede representar una desregulación de la homeóstasis celular al generar procesos tóxicos, por esto se hace necesario evaluar la exposición a las diferentes propuestas de ingeniería en todos los campos del desarrollo. Teniendo en cuenta esto, el objetivo del estudio fue evaluar la citotoxicidad y genotoxicidad del material orgánico extraíble (MOE) proveniente de diésel y diésel con mezclas de alcoholes y nanopartículas (NPs) del copolímero de ácido láctico y glicólico (PLGA) y la expresión diferencial de proteínas tras la exposición al MOE. Esto se logró llevando a cabo el ensayo de MTT para determinar la citotoxicidad de estos compuestos, para la evaluación de genotoxicidad se realizó el ensayo de electroforesis alcalino de células individuales (ensayo Cometa) y para la evaluación de la mutagenicidad de Nps se realizó el test de AMES. Posteriormente, para evaluar la expresión diferencial de proteínas, se realizó la extracción de proteínas totales y mediante geles de una dimensión (1D) se evaluó integridad y estabilidad, para la generación de los perfiles proteicos se realizó una electroforesis de dos dimensiones (2D) y las proteínas que se encontraron diferencialmente expresadas frente al control negativo se caracterizaron mediante espectrometría de masas (MALDI-TOF/TOF). Los resultados indicaron efectos genotóxicos, pero no citotóxicos tras la exposición a MP y Nps y no se evidenció mutagenicidad mediada por Nps. En cuanto a la expresión diferencial de proteínas tras la exposición al MOE, se evidenció sobreexpresión de proteínas relacionadas con el estrés oxidativo. En conjunto estos resultados resaltan la necesidad de probar nuevas tecnologías y compuestos a los cuales pueden expuestos los sistemas biológicos. (Texto tomado de la fuente)The particulate matter (PM) of diesel fuels is a complex mixture that can vary depending on the characteristics of the engine, its type, the type of fuel and the source of production, it has been shown to have negative effects on environmental health and For this reason, the need has been generated to test alternative fuels that may have less effect on biological systems, taking this into account, the cytotoxicity and genotoxicity of the organic fraction of the particulate material of engines fed with alternative fuels corresponding to diesel was evaluated. . Mixed with alcohols (10% ethanol and butanol) by in vitro tests using the HepG2 cell line, cytotoxicity was evaluated by the MTT colorimetric test and genotoxicity by the comet test. A low cytotoxicity of the extracts was found, but a high genotoxicity, showing statistically significant differences between the negative control and the different treatments in all the concentrations tested, with this it is concluded that the organic fraction of the PM has a genotoxic effect that can lead to imbalances of cellular homeostasis.MaestríaMagister en Ciencias-biotecnologíaInvestigaciónMutagénesis y Epigenética AmbientaÁrea Curricular de Bioctecnologíaxiv, 66 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Maestría en Ciencias - BiotecnologíaEscuela de biocienciasFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín660 - Ingeniería químicaCitotoxicidad por mediación celularCell-mediated cytotoxicityToxicoproteómicaNanopartículasDiésel con mezcla de alcoholesToxicoproteomicsNanoparticlesDiesel with a mixture of alcoholsEvaluación in vitro del efecto del material particulado y un potencial tratamiento en dos líneas celularesIn vitro evaluation of the effect of particulate matter and a potential treatment on two cell linesTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMYadav R, Sahu LK, Beig G, Tripathi N, Jaaffrey SNA. 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From the Cover: Zinc oxide Nanoparticles-Induced Reactive Oxygen Species Promotes Multimodal Cyto- and Epigenetic Toxicity. Toxicol Sci. 01 de 2017;156(1):261-74.InvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-84675https://repositorio.unal.edu.co/bitstream/unal/82250/1/license.txtb577153cc0e11f0aeb5fc5005dc82d8aMD51ORIGINAL1214714626.2021.pdf1214714626.2021.pdfTesis de Maestría en Ciencias - Biotecnologíaapplication/pdf1528409https://repositorio.unal.edu.co/bitstream/unal/82250/3/1214714626.2021.pdfabd4ba7996320f3c16150593e742fef6MD53THUMBNAIL1214714626.2021.pdf.jpg1214714626.2021.pdf.jpgGenerated Thumbnailimage/jpeg4148https://repositorio.unal.edu.co/bitstream/unal/82250/4/1214714626.2021.pdf.jpg1a24db62da76af7835258cef3aede6acMD54unal/82250oai:repositorio.unal.edu.co:unal/822502024-08-11 01:07:18.774Repositorio Institucional Universidad Nacional de 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