Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.

Potato virus S (PVS) is a prevalent virus in potato fields in Colombia and the rest of the world. PVS has been classified into two separate lineages, PVSO (Ordinary) and PVSA (Andean), which are genetically distinct. In this study, the complete genome sequence of a new PVS isolate (PVS_Antioquia) wa...

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Autores:
Vallejo C., Daniela
Gutiérrez S., Pablo Andrés
Marín M., Mauricio
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/58544
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/58544
http://bdigital.unal.edu.co/55327/
Palabra clave:
57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
plant viruses
carlaviruses
diagnostic techniques
ELISA
PCR
potatoes
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_7a6d360ece0f3c311b4bb1abbcd0a634
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Vallejo C., Daniela0d258340-f436-4468-903b-39af2a064377300Gutiérrez S., Pablo Andrésb5fcdce7-99d4-4085-b5ae-e2c7740de094300Marín M., Mauricio3e708e81-5aa1-4a43-a342-acf033e3a0083002019-07-02T14:21:02Z2019-07-02T14:21:02Z2016-01-01ISSN: 2357-3732https://repositorio.unal.edu.co/handle/unal/58544http://bdigital.unal.edu.co/55327/Potato virus S (PVS) is a prevalent virus in potato fields in Colombia and the rest of the world. PVS has been classified into two separate lineages, PVSO (Ordinary) and PVSA (Andean), which are genetically distinct. In this study, the complete genome sequence of a new PVS isolate (PVS_Antioquia) was obtained using High-throughput sequencing (Illumina HiSeq-2000) from tuber sprouts of Solanum phureja (var. Criolla Colombia). The PVS_Antioquia genome comprises 8,483 nt that code for six ORFs: RdRp (223 kDa), TGBp1-3 (25kDa, 12kDa, 7kDa) CP (32.3 kDa) and NABP (11 kDa) and share a high sequence identity with respect to the PVS_RVC (95%) from Colombia, in contrast to 81 to 82% identity with respect to the PVSA and PVSO isolates from around the world. This genome information was used to design RT-qPCR primers that are specific for the Colombian PVS strains (RVC and Antioquia) which were validated in S. phureja leaf and tuber samples. These primers detected PVS in 80 and 60% of a set of fifteen leaf and tuber samples, respectively, suggesting a high incidence of this virus in the potato crops of Antioquia.application/pdfspaUniversidad Nacional de Colombiahttp://www.revistas.unal.edu.co/index.php/agrocol/article/view/53161Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaVallejo C., Daniela and Gutiérrez S., Pablo Andrés and Marín M., Mauricio (2016) Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk. Agronomía Colombiana, 34 (1). pp. 51-60. ISSN 2357-373257 Ciencias de la vida; Biología / Life sciences; biology58 Plantas / Plantsplant virusescarlavirusesdiagnostic techniquesELISAPCRpotatoesGenome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.Artículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINALagron.colomb.v34n1.53161.pdfapplication/pdf372034https://repositorio.unal.edu.co/bitstream/unal/58544/1/agron.colomb.v34n1.53161.pdfb5b574d577aedcdf6828486c29ab85d6MD51THUMBNAILagron.colomb.v34n1.53161.pdf.jpgagron.colomb.v34n1.53161.pdf.jpgGenerated Thumbnailimage/jpeg9157https://repositorio.unal.edu.co/bitstream/unal/58544/2/agron.colomb.v34n1.53161.pdf.jpg75a8a4b501dde89f0d3e9cc8ef5f39b6MD52unal/58544oai:repositorio.unal.edu.co:unal/585442023-03-28 23:07:48.503Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
title Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
spellingShingle Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
plant viruses
carlaviruses
diagnostic techniques
ELISA
PCR
potatoes
title_short Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
title_full Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
title_fullStr Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
title_full_unstemmed Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
title_sort Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk.
dc.creator.fl_str_mv Vallejo C., Daniela
Gutiérrez S., Pablo Andrés
Marín M., Mauricio
dc.contributor.author.spa.fl_str_mv Vallejo C., Daniela
Gutiérrez S., Pablo Andrés
Marín M., Mauricio
dc.subject.ddc.spa.fl_str_mv 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
topic 57 Ciencias de la vida; Biología / Life sciences; biology
58 Plantas / Plants
plant viruses
carlaviruses
diagnostic techniques
ELISA
PCR
potatoes
dc.subject.proposal.spa.fl_str_mv plant viruses
carlaviruses
diagnostic techniques
ELISA
PCR
potatoes
description Potato virus S (PVS) is a prevalent virus in potato fields in Colombia and the rest of the world. PVS has been classified into two separate lineages, PVSO (Ordinary) and PVSA (Andean), which are genetically distinct. In this study, the complete genome sequence of a new PVS isolate (PVS_Antioquia) was obtained using High-throughput sequencing (Illumina HiSeq-2000) from tuber sprouts of Solanum phureja (var. Criolla Colombia). The PVS_Antioquia genome comprises 8,483 nt that code for six ORFs: RdRp (223 kDa), TGBp1-3 (25kDa, 12kDa, 7kDa) CP (32.3 kDa) and NABP (11 kDa) and share a high sequence identity with respect to the PVS_RVC (95%) from Colombia, in contrast to 81 to 82% identity with respect to the PVSA and PVSO isolates from around the world. This genome information was used to design RT-qPCR primers that are specific for the Colombian PVS strains (RVC and Antioquia) which were validated in S. phureja leaf and tuber samples. These primers detected PVS in 80 and 60% of a set of fifteen leaf and tuber samples, respectively, suggesting a high incidence of this virus in the potato crops of Antioquia.
publishDate 2016
dc.date.issued.spa.fl_str_mv 2016-01-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T14:21:02Z
dc.date.available.spa.fl_str_mv 2019-07-02T14:21:02Z
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format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.issn.spa.fl_str_mv ISSN: 2357-3732
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/58544
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/55327/
identifier_str_mv ISSN: 2357-3732
url https://repositorio.unal.edu.co/handle/unal/58544
http://bdigital.unal.edu.co/55327/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://www.revistas.unal.edu.co/index.php/agrocol/article/view/53161
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
Agronomía Colombiana
dc.relation.references.spa.fl_str_mv Vallejo C., Daniela and Gutiérrez S., Pablo Andrés and Marín M., Mauricio (2016) Genome characterization of a Potato virus S (PVS) variant from tuber sprouts of Solanum phureja Juz. et Buk. Agronomía Colombiana, 34 (1). pp. 51-60. ISSN 2357-3732
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
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dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
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dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
institution Universidad Nacional de Colombia
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