Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues

ilustraciones, diagramas, fotografías

Autores:: Silva Castellanos, Christian Augusto

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: eng

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dc.title.eng.fl_str_mv	Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues
dc.title.translated.spa.fl_str_mv	Extrusor para bioimpresión 3D con biotinta compuesta orientado a la evaluación de viabilidad celular en la generación de tejidos
title	Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues
spellingShingle	Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues 620 - Ingeniería y operaciones afines::629 - Otras ramas de la ingeniería 610 - Medicina y salud::615 - Farmacología y terapéutica Supervivencia Celular Bioimpresión/instrumentación Ingeniería de Tejidos/métodos Cell Survival Bioprinting/instrumentation Tissue Engineering/methods 3D bioprinting Coaxial printing Vascularized tissues Tissue-engineered vascular grafts Cell viability Bioimpresión 3D Impresión coaxial Tejidos vascularizados Ingeniería tisular Viabilidad celular
title_short	Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues
title_full	Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues
title_fullStr	Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues
title_full_unstemmed	Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues
title_sort	Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues
dc.creator.fl_str_mv	Silva Castellanos, Christian Augusto
dc.contributor.advisor.spa.fl_str_mv	Cortés Rodríguez, Carlos Julio
dc.contributor.author.spa.fl_str_mv	Silva Castellanos, Christian Augusto
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigación en Biomecánica / Universidad Nacional de Colombia Gibm-Uncb
dc.contributor.cvlac.spa.fl_str_mv	Silva Castellanos, Christian Augusto [0001390041]
dc.contributor.researchgate.spa.fl_str_mv	https://www.researchgate.net/profile/Christian-Silva-15
dc.contributor.googlescholar.spa.fl_str_mv	Silva Castellanos, Christian Augusto [9t8wUxMAAAAJ&hl]
dc.subject.ddc.spa.fl_str_mv	620 - Ingeniería y operaciones afines::629 - Otras ramas de la ingeniería 610 - Medicina y salud::615 - Farmacología y terapéutica
topic	620 - Ingeniería y operaciones afines::629 - Otras ramas de la ingeniería 610 - Medicina y salud::615 - Farmacología y terapéutica Supervivencia Celular Bioimpresión/instrumentación Ingeniería de Tejidos/métodos Cell Survival Bioprinting/instrumentation Tissue Engineering/methods 3D bioprinting Coaxial printing Vascularized tissues Tissue-engineered vascular grafts Cell viability Bioimpresión 3D Impresión coaxial Tejidos vascularizados Ingeniería tisular Viabilidad celular
dc.subject.decs.spa.fl_str_mv	Supervivencia Celular Bioimpresión/instrumentación Ingeniería de Tejidos/métodos
dc.subject.decs.eng.fl_str_mv	Cell Survival Bioprinting/instrumentation Tissue Engineering/methods
dc.subject.proposal.eng.fl_str_mv	3D bioprinting Coaxial printing Vascularized tissues Tissue-engineered vascular grafts Cell viability
dc.subject.proposal.spa.fl_str_mv	Bioimpresión 3D Impresión coaxial Tejidos vascularizados Ingeniería tisular Viabilidad celular
description	ilustraciones, diagramas, fotografías
publishDate	2023
dc.date.issued.none.fl_str_mv	2023-02-01
dc.date.accessioned.none.fl_str_mv	2024-07-19T13:56:02Z
dc.date.available.none.fl_str_mv	2024-07-19T13:56:02Z
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/86572
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/86572 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	eng
language	eng
dc.relation.indexed.spa.fl_str_mv	Bireme
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dc.format.extent.spa.fl_str_mv	xxviii, 199 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
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dc.publisher.faculty.spa.fl_str_mv	Facultad de Ingeniería
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Cortés Rodríguez, Carlos Julio48fe60e7734d42e4e2cd46b83acff1c3Silva Castellanos, Christian Augustoac89f1406d8a93bd6e5d6e8b27c9a1d4Grupo de Investigación en Biomecánica / Universidad Nacional de Colombia Gibm-UncbSilva Castellanos, Christian Augusto [0001390041]https://www.researchgate.net/profile/Christian-Silva-15Silva Castellanos, Christian Augusto [9t8wUxMAAAAJ&hl]2024-07-19T13:56:02Z2024-07-19T13:56:02Z2023-02-01https://repositorio.unal.edu.co/handle/unal/86572Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografías3D bioprinting is an emerging biofabrication strategy that utilizes bioinks and models generated with CAD-like tools for the automated fabrication of tissue scaffolds and organlike constructs. Despite recent advances in materials and techniques with significant potential to achieve the fabrication of tissues relevant for clinical and in vitro applications, various aspects, such as tissue vascularization and prolonged cell functionality, are limited by the advancements in this field. Among the different 3D bioprinting techniques, extrusionbased bioprinting (EBB) has been conceived as the most promising for achieving this goal due to its versatility and availability. This document reports on developing three- and fourlayer extrusion systems axially aligned to overcome the current limitations faced when attempting to manufacture vascularized tissues and stable, perfusable vascular structures. We combined in silico simulations with in vitro experiments to precisely design multiple axial layered tissue extrusion systems with a high degree of cellular viability and versatility for 3D bioprinting applications. Furthermore, we report the hardware and software modifications made on commercially available 3D printers and bioprinters to allow the simultaneous deposition of multiple materials using coaxial nozzles. Finally, we demonstrate the versatility and potential of the four-layer coaxial extrusion system by printing perfusable vascular constructs and vascular networks with some commercially available bioinks. Our work paves the way for the rational design of coaxial extrusion systems with enormous potential in manufacturing hollow tubular constructs relevant to mimic structures found in the human body.La bioimpresión 3D es una estrategia de biofabricación emergente que emplea biotintas y modelos generados con herramientas tipo CAD para la fabricación automatizada de andamiajes de tejidos y constructos similares a órganos. A pesar de los avances recientes en materiales y técnicas con gran potencial para lograr la fabricación de tejidos relevantes para aplicaciones clínicas e in vitro, varios aspectos tales como la vascularización de tejidos y la funcionalidad prolongada de las células están limitada a los avances en este campo. Entre las diversas técnicas de bioimpresión 3D, la bioimpresión basada en extrusión (EBB) ha sido concebida como la más prometedora para lograr este objetivo, debido a su versatilidad y disponibilidad. En este documento se informa el desarrollo de sistemas de extrusión de tres y de cuatro capas alineadas axialmente destinados a resolver las limitaciones actuales que se enfrentan al intentar fabricar tejidos vascularizados y estructuras vasculares estables y perfundibles. Combinamos simulaciones in silico con experimentos in vitro para diseñar con precisión múltiples sistemas de extrusión de tejidos en capas axiales con alto grado de viabilidad celular y versatilidad para aplicaciones de bioimpresión 3D. Además, informamos las modificaciones de hardware y software realizadas en impresoras 3D y bioimpresoras disponibles comercialmente para permitir la deposición simultánea de múltiples materiales usando boquillas coaxiales. Finalmente, demostramos la versatilidad y el potencial del sistema de extrusión coaxial de cuatro capas mediante la impresión de constructos vasculares perfundibles y de redes vasculares con algunas biotintas disponibles comercialmente. Nuestro trabajo allana el camino para el diseño racional de sistemas de extrusión coaxial con gran potencial en la fabricación de constructos tubulares huecos relevantes para imitar estructuras que se encuentran en el cuerpo humano. (Texto tomado de la fuente).Colciencias: Doctorado /Ingeniería Mecánica Universidad Nacional de Colombia Bogota, Colombia Doctorado Nacional - 647 Deutscher Akademischer Austauschdienst (DAAD), which financially supported my research internship in Germany.DoctoradoDoctor en IngenieríaBiomechanics and tissue engineeringxxviii, 199 páginasapplication/pdfengUniversidad Nacional de ColombiaBogotá - Ingeniería - Doctorado en Ingeniería - Ingeniería Mecánica y MecatrónicaFacultad de IngenieríaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá620 - Ingeniería y operaciones afines::629 - Otras ramas de la ingeniería610 - Medicina y salud::615 - Farmacología y terapéuticaSupervivencia CelularBioimpresión/instrumentaciónIngeniería de Tejidos/métodosCell SurvivalBioprinting/instrumentationTissue Engineering/methods3D bioprintingCoaxial printingVascularized tissuesTissue-engineered vascular graftsCell viabilityBioimpresión 3DImpresión coaxialTejidos vascularizadosIngeniería tisularViabilidad celularExtruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissuesExtrusor para bioimpresión 3D con biotinta compuesta orientado a la evaluación de viabilidad celular en la generación de tejidosTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDBiremeS. 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March, pp. 1–18, 2021, doi: 10.3389/fendo.2021.634415.ColcienciasDAADInvestigadoresORIGINAL80796285.2024.pdf80796285.2024.pdfTesis de Doctorado en Ingeniería - Ingeniería Mecánica y Mecatrónicaapplication/pdf7280868https://repositorio.unal.edu.co/bitstream/unal/86572/2/80796285.2024.pdff0ae545b739a325d7e35cd39e69f1375MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86572/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53THUMBNAIL80796285.2024.pdf.jpg80796285.2024.pdf.jpgGenerated Thumbnailimage/jpeg5162https://repositorio.unal.edu.co/bitstream/unal/86572/4/80796285.2024.pdf.jpg8bd185d6a201ce0e2c31e809a3b51619MD54unal/86572oai:repositorio.unal.edu.co:unal/865722024-08-26 23:10:53.313Repositorio Institucional Universidad Nacional de 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Extruder for 3D bioprinting with composed bioink oriented to the cellular viability evaluation in the generation of tissues

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