Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt

Five experiments were carried outin the laboratory to obtain callus and plantlets from young petals of carnation(Dianthus caryophyilus) using differentconcentrations of BAP, NAA, 2,40 and light/dark treatments. The best results wereobtained with Murashige and Skoog madium containing 0,5 ppm of BAP,...

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Autores:
Álzate M., Ana L.
Angarita Zerda, Antonio
Montes de Gómez, Virginia
Tipo de recurso:
Article of journal
Fecha de publicación:
1990
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/33849
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/33849
http://bdigital.unal.edu.co/23929/
Palabra clave:
58 Plantas / Plants
dianthus caryophyllus
somaclonal
clavel
callos
plántulas
oxysporum f. sp. dianthi
concentraciones hormonales
pétalos
material toxico
liquido
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_767a87bd6ef845c48d8675e4f2603ec1
oai_identifier_str oai:repositorio.unal.edu.co:unal/33849
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Álzate M., Ana L.874dce3a-43b1-4f9f-bce4-e3c9cd4c91c8300Angarita Zerda, Antonio6f1ea8e8-1c1c-43a6-bc40-b6ad527baa5c300Montes de Gómez, Virginia9b88f005-3a0a-470f-a61a-92dfd60f005c3002019-06-27T23:05:14Z2019-06-27T23:05:14Z1990https://repositorio.unal.edu.co/handle/unal/33849http://bdigital.unal.edu.co/23929/Five experiments were carried outin the laboratory to obtain callus and plantlets from young petals of carnation(Dianthus caryophyilus) using differentconcentrations of BAP, NAA, 2,40 and light/dark treatments. The best results wereobtained with Murashige and Skoog madium containing 0,5 ppm of BAP, 2 ppm of NAA and O,1 ppm of 2,4D on light conditions. It was determinated that the whole petal asexplant was better to obtain plantlets regenerated. For the selection pressure it wasused autoclaved toxic material of F. oxysporum f. sp. dianthi (between O and 40%)mixad with the plantlets and callus regenerated medium. From this experiment it was obtained possible tolerants somaclonal variants of carnation to 10% level of toxics materials.Con el objeto de definir las técnicas de un posible método de selección decallos y plántulas de clavel (Dianthus caryophyllus) tolerantes a Fusarium oxysporum f.sp. dianthi, se realizaron en primera instancia experimentos en los cuales se determinaron concentraciones hormonales ideales para, lograr la regeneración de callos y plántulas apartir de pétalos. El medio ideal obtenido, quedó constituido por el medio de Murashige y Skoog, BAP: 0.5 ppm, NAA: 2 ppm y 2,4 D: 0.1 ppm. Se determinó el pétalo comopleto como el mejor explante en el logro dela regeneración de plántulas. Para ejercer presión de selección se utilizaron concentraciones de material tóxico liquido autoclavado de F. oxysporum entre O - 40% combinado con el medio de regeneración de callos y plántulas. De este último experimento se obtuvieron posibles variantes somaclonales tolerantes hasta niveles del 10% de material tóxico producido por el patógeno.application/pdfspaUniversidad Nacional de Colombia, Facultad de Agronomía, Centro Editorialhttp://revistas.unal.edu.co/index.php/agrocol/article/view/21016Universidad Nacional de Colombia Revistas electrónicas UN Agronomía ColombianaAgronomía ColombianaAgronomía Colombiana; Vol. 7, núm. 1-2 (1990); 118-127 Agronomía Colombiana; Vol. 7, núm. 1-2 (1990); 118-127 2357-3732 0120-9965Álzate M., Ana L. and Angarita Zerda, Antonio and Montes de Gómez, Virginia (1990) Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt. Agronomía Colombiana; Vol. 7, núm. 1-2 (1990); 118-127 Agronomía Colombiana; Vol. 7, núm. 1-2 (1990); 118-127 2357-3732 0120-9965 .58 Plantas / Plantsdianthus caryophyllussomaclonalclavelcallosplántulasoxysporum f. sp. dianthiconcentraciones hormonalespétalosmaterial toxicoliquidoPreliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wiltArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL21016-71164-1-PB.pdfapplication/pdf614815https://repositorio.unal.edu.co/bitstream/unal/33849/1/21016-71164-1-PB.pdf495b4cc063a08e2d9ff5aef2a44380f4MD51THUMBNAIL21016-71164-1-PB.pdf.jpg21016-71164-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg11320https://repositorio.unal.edu.co/bitstream/unal/33849/2/21016-71164-1-PB.pdf.jpg5e04d452dfd8fec5f83bf94ec46b2cacMD52unal/33849oai:repositorio.unal.edu.co:unal/338492023-12-23 23:05:48.124Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt
title Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt
spellingShingle Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt
58 Plantas / Plants
dianthus caryophyllus
somaclonal
clavel
callos
plántulas
oxysporum f. sp. dianthi
concentraciones hormonales
pétalos
material toxico
liquido
title_short Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt
title_full Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt
title_fullStr Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt
title_full_unstemmed Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt
title_sort Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt
dc.creator.fl_str_mv Álzate M., Ana L.
Angarita Zerda, Antonio
Montes de Gómez, Virginia
dc.contributor.author.spa.fl_str_mv Álzate M., Ana L.
Angarita Zerda, Antonio
Montes de Gómez, Virginia
dc.subject.ddc.spa.fl_str_mv 58 Plantas / Plants
topic 58 Plantas / Plants
dianthus caryophyllus
somaclonal
clavel
callos
plántulas
oxysporum f. sp. dianthi
concentraciones hormonales
pétalos
material toxico
liquido
dc.subject.proposal.spa.fl_str_mv dianthus caryophyllus
somaclonal
clavel
callos
plántulas
oxysporum f. sp. dianthi
concentraciones hormonales
pétalos
material toxico
liquido
description Five experiments were carried outin the laboratory to obtain callus and plantlets from young petals of carnation(Dianthus caryophyilus) using differentconcentrations of BAP, NAA, 2,40 and light/dark treatments. The best results wereobtained with Murashige and Skoog madium containing 0,5 ppm of BAP, 2 ppm of NAA and O,1 ppm of 2,4D on light conditions. It was determinated that the whole petal asexplant was better to obtain plantlets regenerated. For the selection pressure it wasused autoclaved toxic material of F. oxysporum f. sp. dianthi (between O and 40%)mixad with the plantlets and callus regenerated medium. From this experiment it was obtained possible tolerants somaclonal variants of carnation to 10% level of toxics materials.
publishDate 1990
dc.date.issued.spa.fl_str_mv 1990
dc.date.accessioned.spa.fl_str_mv 2019-06-27T23:05:14Z
dc.date.available.spa.fl_str_mv 2019-06-27T23:05:14Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/ART
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/33849
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/23929/
url https://repositorio.unal.edu.co/handle/unal/33849
http://bdigital.unal.edu.co/23929/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/agrocol/article/view/21016
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Agronomía Colombiana
Agronomía Colombiana
dc.relation.ispartofseries.none.fl_str_mv Agronomía Colombiana; Vol. 7, núm. 1-2 (1990); 118-127 Agronomía Colombiana; Vol. 7, núm. 1-2 (1990); 118-127 2357-3732 0120-9965
dc.relation.references.spa.fl_str_mv Álzate M., Ana L. and Angarita Zerda, Antonio and Montes de Gómez, Virginia (1990) Preliminar studies on somaclonal variation of carnation (dianthus caryophyllus), and "in vitro" selection of calluses and plantlets resistants to fusarial wilt. Agronomía Colombiana; Vol. 7, núm. 1-2 (1990); 118-127 Agronomía Colombiana; Vol. 7, núm. 1-2 (1990); 118-127 2357-3732 0120-9965 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia, Facultad de Agronomía, Centro Editorial
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/33849/1/21016-71164-1-PB.pdf
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repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
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