Complejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)

ilustraciones, fotografías, gráficas, tablas

Autores:
Rodríguez Parra, John Alexander
Tipo de recurso:
Fecha de publicación:
2020
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/79094
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/79094
https://repositorio.unal.edu.co/
Palabra clave:
630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
Podredumbres
Enfermedades de las plantas
Bacterias patógenas
rots
plant diseases
pathogenic bacteria
Dickeya solani
Pectobacterium atrosepticum
Pseudomonas asplenii
Diagnóstico
Ornamentales
Dickeya solani
Pectobacterium atrosepticum
Pseudomonas asplenii
Diagnoses
Ornamentals
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_73d4b5461b3fcb171644950f3ea8f839
oai_identifier_str oai:repositorio.unal.edu.co:unal/79094
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Complejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)
title Complejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)
spellingShingle Complejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)
630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
Podredumbres
Enfermedades de las plantas
Bacterias patógenas
rots
plant diseases
pathogenic bacteria
Dickeya solani
Pectobacterium atrosepticum
Pseudomonas asplenii
Diagnóstico
Ornamentales
Dickeya solani
Pectobacterium atrosepticum
Pseudomonas asplenii
Diagnoses
Ornamentals
title_short Complejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)
title_full Complejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)
title_fullStr Complejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)
title_full_unstemmed Complejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)
title_sort Complejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)
dc.creator.fl_str_mv Rodríguez Parra, John Alexander
dc.contributor.advisor.spa.fl_str_mv Gonzalez Almario, Adriana
dc.contributor.author.spa.fl_str_mv Rodríguez Parra, John Alexander
dc.contributor.researchgroup.spa.fl_str_mv Genética de Rasgos de Interés Agronómico
dc.subject.ddc.spa.fl_str_mv 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
topic 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
Podredumbres
Enfermedades de las plantas
Bacterias patógenas
rots
plant diseases
pathogenic bacteria
Dickeya solani
Pectobacterium atrosepticum
Pseudomonas asplenii
Diagnóstico
Ornamentales
Dickeya solani
Pectobacterium atrosepticum
Pseudomonas asplenii
Diagnoses
Ornamentals
dc.subject.agrovoc.spa.fl_str_mv Podredumbres
Enfermedades de las plantas
Bacterias patógenas
dc.subject.agrovoc.eng.fl_str_mv rots
plant diseases
pathogenic bacteria
dc.subject.proposal.spa.fl_str_mv Dickeya solani
Pectobacterium atrosepticum
Pseudomonas asplenii
Diagnóstico
Ornamentales
dc.subject.proposal.eng.fl_str_mv Dickeya solani
Pectobacterium atrosepticum
Pseudomonas asplenii
Diagnoses
Ornamentals
description ilustraciones, fotografías, gráficas, tablas
publishDate 2020
dc.date.issued.spa.fl_str_mv 2020
dc.date.accessioned.spa.fl_str_mv 2021-02-05T17:30:27Z
dc.date.available.spa.fl_str_mv 2021-02-05T17:30:27Z
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/79094
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.none.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/79094
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gonzalez Almario, Adriana2095714a2ee0a49a87d2319bc2e287b4Rodríguez Parra, John Alexander01a4d7d86ee37dea3ea8cea2713d9176Genética de Rasgos de Interés Agronómico2021-02-05T17:30:27Z2021-02-05T17:30:27Z2020https://repositorio.unal.edu.co/handle/unal/79094Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficas, tablasLa pudrición blanda bacteriana de ciclamen (Cyclamen persicum Mill.) es un problema fitosanitario que ocasiona pérdidas del 20% en producción en el mundo y en Colombia se desconocen sus síntomas y agentes causales. Como objetivo de esta investigación se planteó describir el desarrollo de esta enfermedad con base en su sintomatología e identificar a nivel de especie sus agentes causales. Para ello, se visitaron viveros del municipio de San Antonio del Tequendama, Colombia, donde se seleccionaron plantas enfermas para la descripción de los síntomas, realizar los aislamientos y caracterizarlos morfológica, bioquímica y molecularmente, además de evaluar su actividad pectolítica en papa y su patogenicidad en plantas de ciclamen. Como resultado, se asociaron seis síntomas con la enfermedad: marchitez generalizada, cormo blando, presencia de exudado en el cormo, pudrición blanda de raíces, pudriciones blandas y pudriciones blandas necróticas en peciolos y pedúnculos; de los cuales 47 morfotipos fueron aislados siendo siete patógenicos, uno provenía de síntomas de cormos blandos, dos de peciolos con pudriciones blandas, dos de peciolos con pudriciones blandas necróticas y dos de pudriciones blandas en raiz. Un análisis de correspondencia múltiple considerando las características, morfológicas, bioquímicas y patogénicas, agrupó los siete aislamientos en los géneros Pseudomonas, Dickeya y Pectobacterium. Un análisis multilocus (MLSA) a partir de las secuencias de la región del 16S rADN y housekeeping genes (gapA, icdA, mdh, pgi, rpoB y rpoD) confirmó las especies bacterianas, Pseudomonas asplenii, Dickeya solani y Pectobacterium atrosepticum como los agentes causales de la pudrición blanda bacteriana del ciclamen en Colombia. (Texto tomado de la fuente).The cyclamen bacterial soft rot (Cyclamen persicum Mill.) is a phytosanitary problem that causes 20% production losses worldwide, however in Colombia its symptoms and causal agents are unknown. The aim of this research was the description of the bacterial soft rot disease development, based on symptomatology, and the identification to the species level of the causal bacterial agents. For that, nurseries of the San Antonio del Tequendama municipality in Colombia were visited, diseased plants were selected to describe the symptoms, isolate the pathogens, and characterize them morphological, biochemical and molecularly, besides evaluating its pectolytic activity on potato and pathogenicity on cyclamen plants. As a result, six symptoms were associated with the disease: wilt, soft corm, exudate on corm, rot roots, soft rot and necrotic soft rot on petioles and peduncles. Seven morphotypes, out of 47 isolated were pathogenic; one from the soft corms, two from petioles with soft rot, two from petioles necrotic soft rot, and two from root rot. Considering the morphological, biochemical and pathogenic characteristics, a multiple correspondence analysis clustered the seven isolates in the genera Pseudomonas, Dickeya and Pectobacterium. From the sequences of the 16S rADN region and housekeeping genes (gapA, icdA, mdh, pgi, rpoB y rpoD), a multi-locus analysis (MLSA) confirmed the bacterial species, Pseudomonas asplenii, Dickeya solani and Pectobacterium atrosepticum as the causal agents of the cyclamen bacterial soft rot in Colombia.Proyectos de investigación entre la Universidad de Cundinamarca y la Universidad Nacional de Colombia, Bogotá, 2017Identificación del agente etiológico de la pudrición bacteriana de violeta de los Alpes (Cyclamen persicum Mill.), No. 39398Incluye anexosMaestríaMagíster en Ciencias AgrariasFitopatologiaCiencias Agronómicasxvii, 94 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias Agrarias - Maestría en Ciencias AgrariasEscuela de posgradosFacultad de Ciencias AgrariasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetalesPodredumbresEnfermedades de las plantasBacterias patógenasrotsplant diseasespathogenic bacteriaDickeya solaniPectobacterium atrosepticumPseudomonas aspleniiDiagnósticoOrnamentalesDickeya solaniPectobacterium atrosepticumPseudomonas aspleniiDiagnosesOrnamentalsComplejo bacteriano asociado a la pudrición blanda de ciclamen (Cyclamen persicum Mill.)Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAdeolu, M., Alnajar, S., Naushad, S., y S. Gupta, R. (2016). Genome-based phylogeny and taxonomy of the ‘Enterobacteriales’: Proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. International Journal of Systematic and Evolutionary Microbiology, 66(12), 5575-5599. https://doi.org/10.1099/ijsem.0.001485Alcaldia Municipal de San Antonio del Tequendama. (2016). Plan de Desarrollo Territorial municipio de San Antonio del Tequendama «Lucho por San Antonio». calameo.com. https://www.calameo.com/read/004849162415ea9509df8Amani, B. (1967). Soft rot of ornamentals and vegetables. Iranian Journal of Plant Pathology, 4, 1-10.Beaumont, A. (1953). Cyclamen diseases. Gardeners’ chronicle, 133-240.Brady, C. L., Cleenwerck, I., Denman, S., Venter, S. N., Rodríguez-Palenzuela, P., Coutinho, T. A., y De Vos, P. (2012). 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Genomic and metabolic comparison with Dickeya dadantii 3937 reveals the emerging Dickeya solani potato pathogen to display distinctive metabolic activities and T5SS/T6SS-related toxin repertoire. BMC Genomics, 15, 283. https://doi.org/10.1186/1471-2164-15-283InvestigadoresEstudiantesPúblico generalORIGINAL80833418.2021.pdf80833418.2021.pdfTesis de Maestría en Ciencias Agrariasapplication/pdf43540337https://repositorio.unal.edu.co/bitstream/unal/79094/1/80833418.2021.pdf5c706c8a2b183f026c93325a4c38fed7MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8701https://repositorio.unal.edu.co/bitstream/unal/79094/3/license_rdf42fd4ad1e89814f5e4a476b409eb708cMD53LICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/79094/2/license.txtcccfe52f796b7c63423298c2d3365fc6MD52THUMBNAIL80833418.2021.pdf.jpg80833418.2021.pdf.jpgGenerated Thumbnailimage/jpeg5040https://repositorio.unal.edu.co/bitstream/unal/79094/4/80833418.2021.pdf.jpg4828291ad0ab61439af1009a59b08935MD54unal/79094oai:repositorio.unal.edu.co:unal/790942024-07-08 23:39:40.145Repositorio Institucional Universidad Nacional de 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GVyZWNob3MgZGUgYXV0b3IgcXVlIGNvbmxsZXZlIGxhIGRpc3RyaWJ1Y2nDs24gZGUgZXN0b3MgYXJjaGl2b3MgeSBtZXRhZGF0b3MuCkFsIGhhY2VyIGNsaWMgZW4gZWwgc2lndWllbnRlIGJvdMOzbiwgdXN0ZWQgaW5kaWNhIHF1ZSBlc3TDoSBkZSBhY3VlcmRvIGNvbiBlc3RvcyB0w6lybWlub3MuCgpVTklWRVJTSURBRCBOQUNJT05BTCBERSBDT0xPTUJJQSAtIMOabHRpbWEgbW9kaWZpY2FjacOzbiAyNy8yMC8yMDIwCg==

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