Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad

Ilustraciones

Autores:: Torres Bonilla, Javier Mauricio

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2024

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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dc.title.spa.fl_str_mv	Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad
dc.title.translated.eng.fl_str_mv	Genomic and transcryptomic analysis of the interaction musa - pseudocercospora fijiensis as tool in the identification and characterization of fungal genes of virulence and pathogenicity
title	Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad
spellingShingle	Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad 570 - Biología Banano - Enfermedades y plagas Genes de patogenicidad Genómica de hongos Banano Sigatoka negra Hongo Mycosphaerella fijiensis Black Sigatoka Pathogenicity genes Banana Fungal Genomics Mycosphaerella fijiensis Patogénesis
title_short	Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad
title_full	Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad
title_fullStr	Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad
title_full_unstemmed	Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad
title_sort	Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad
dc.creator.fl_str_mv	Torres Bonilla, Javier Mauricio
dc.contributor.advisor.none.fl_str_mv	Arango Isaza, Rafael Eduardo Morales Osorio, Juan Gonzalo
dc.contributor.author.none.fl_str_mv	Torres Bonilla, Javier Mauricio
dc.contributor.researchgroup.spa.fl_str_mv	Biotecnología Vegetal UNALMED-CIB
dc.contributor.orcid.spa.fl_str_mv	Torres Bonilla, Javier Mauricio [0000000172138672]
dc.contributor.cvlac.spa.fl_str_mv	https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000004618
dc.contributor.googlescholar.spa.fl_str_mv	https://scholar.google.com/citations?user=Q2KIJBgAAAAJ&hl=es
dc.subject.ddc.spa.fl_str_mv	570 - Biología
topic	570 - Biología Banano - Enfermedades y plagas Genes de patogenicidad Genómica de hongos Banano Sigatoka negra Hongo Mycosphaerella fijiensis Black Sigatoka Pathogenicity genes Banana Fungal Genomics Mycosphaerella fijiensis Patogénesis
dc.subject.lemb.none.fl_str_mv	Banano - Enfermedades y plagas
dc.subject.proposal.spa.fl_str_mv	Genes de patogenicidad Genómica de hongos Banano Sigatoka negra Hongo Mycosphaerella fijiensis
dc.subject.proposal.eng.fl_str_mv	Black Sigatoka Pathogenicity genes Banana Fungal Genomics
dc.subject.wikidata.none.fl_str_mv	Mycosphaerella fijiensis Patogénesis
description	Ilustraciones
publishDate	2024
dc.date.accessioned.none.fl_str_mv	2024-06-17T14:37:03Z
dc.date.available.none.fl_str_mv	2024-06-17T14:37:03Z
dc.date.issued.none.fl_str_mv	2024-03-20
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/86241
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/86241 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
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dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellín
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Arango Isaza, Rafael Eduardob7e61322453072ebf7408a0f587e39c1Morales Osorio, Juan Gonzaloce9d0b85a9448c46affc4098c5e44864Torres Bonilla, Javier Mauricio315aa45692339fe7f1e42ff1588fbab0600Biotecnología Vegetal UNALMED-CIBTorres Bonilla, Javier Mauricio [0000000172138672]https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000004618https://scholar.google.com/citations?user=Q2KIJBgAAAAJ&hl=es2024-06-17T14:37:03Z2024-06-17T14:37:03Z2024-03-20https://repositorio.unal.edu.co/handle/unal/86241Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/IlustracionesLa Sigatoka negra causada por el hongo Mycosphaerella fijiensis, sinónimo de Pseudocercóspora fijiensis es la enfermedad más importante en el cultivo del banano en el mundo. La enfermedad afecta la capacidad fotosintética de planta y posteriormente el tamaño y calidad de la fruta, generando grandes pérdidas económicas. Son pocos los estudios genéticos reportados que permiten entender cómo el hongo enferma a la planta y cómo evade las defensas de esta; para ello es necesario identificar en el hongo genes candidatos asociados con la patogenicidad y la virulencia. En este trabajo se empleó un análisis bioinformático y de microarreglos para identificar en el hongo la presencia de proteínas secretadas ricas en cisteína (SSPs) con expresión diferencial durante la interacción incompatible planta - P. fijiensis. Adicionalmente, se realizó una búsqueda sistemática de genes mediante el ensamble y anotación de 27 genomas de P. fijiensis, P. musae y P. eumusae. Se encontraron 12 genes codificantes para SSPs con expresión diferencial, además, se encontraron 5 genes asociados con patogenicidad, 7 genes asociados con el desarrollo del patógeno y 7 genes asociados con la biosíntesis de la melanina. Una vez identificados, se procedió con la evaluación funcional de dos genes candidatos mediante nocauts para los genes SD y 85220, además de permitir la evaluación de un método de disrupción génica mediado por Agrobacterium tumefaciens, con lo cual se estableció un protocolo efectivo de evaluación génica, nuestros resultados posibilitan la incursión hacia futuros trabajos de análisis poblacional o de genes con importancia fisiológica en P. fijiensis. (texto tomado de la fuene)Black Sigatoka caused by the fungus Mycosphaerella fijiensis (anamorph Pseudocercospora fijiensis), is the most important disease in banana cultivation worldwide. The disease affects the plant's photosynthetic capacity and subsequently the size and quality of the fruit, leading to significant economic losses. There are few reported genetic studies that help understand how the fungus infects the plant and evades its defenses; therefore, it is necessary to identify candidate genes associated with pathogenicity and virulence in the fungus. In this study, a bioinformatic and microarray analysis was used to identify secreted cysteine-rich proteins (SSPs) with differential expression during the incompatible interaction between the plant and P. fijiensis. Additionally, a systematic search for genes was conducted by assembling and annotating 27 genomes of P. fijiensis, P. musae, and P. eumusae. Twelve genes encoding for SSPs with differential expression were found, as well as five genes associated with pathogenicity, seven genes associated with the pathogen's development, and seven genes associated with melanin biosynthesis. Once identified, two candidate genes were functionally evaluated through knockout experiments for the SD and 85220 genes, in addition to assessing a gene disruption method mediated by Agrobacterium tumefaciens. This established an effective protocol for genetic evaluation, and our results enable future population analysis or studies of genes with physiological importance in P. fijiensis.DoctoradoDoctor en BiotecnologíaLínea patosistema mycosphaerella fijiensisÁrea curricular Biotecnología103 páginasapplication/pdfspaUniversidad Nacional de ColombiaMedellín - Ciencias - Doctorado en BiotecnologíaFacultad de CienciasMedellín, ColombiaUniversidad Nacional de Colombia - Sede Medellín570 - BiologíaBanano - Enfermedades y plagasGenes de patogenicidadGenómica de hongosBananoSigatoka negraHongo Mycosphaerella fijiensisBlack SigatokaPathogenicity genesBananaFungal GenomicsMycosphaerella fijiensisPatogénesisAnálisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidadGenomic and transcryptomic analysis of the interaction musa - pseudocercospora fijiensis as tool in the identification and characterization of fungal genes of virulence and pathogenicityTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAgrios, G. 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Host range of a plant pathogenic fungus determined y a saponin detoxifying enzyme. Science 267, 371-374.CABI (2019). Mycosphaerella fijiensis (black Sigatoka) datasheet. In: Invasive Species Compendium. Wallingford, UK. [online] URL: https://www.cabi.org/isc/datasheet/35278Campo-Arana, R., Vélez-Leiton, S., & Barrera-Violeth, J. (2020). La sigatoka negra Mycosphaerella fijiensis Morelet, en los cultivos de plátano y banano: una revisión. Fitopatología Colombiana, 44(2), 61-66.Canto, B.; Guille, D.; Peraza, L.; Conde, L. y James, A. 2007. Construction and characterization of a bacterial artificial chromosome library of the causal agent of Black Sigatoka fungal leaf spot disease of banana and plantain, Mycosphaerella fijiensis. En: Moliotechnol. 36, Pag 64-70.Carlier, J.; Lebrun, H.; Zapater, M. F.; Dubois, C. y Mourichon, X. 1996. Genetic structure of the global population of banana leaf streak fungus, Mycosphaerella fijiensis. En: Molecular Ecology, 5: 499-510.Chuc-Uc, J. (2007). 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Cell research, 16(1), 31-44.Ministerio de ciencia tecnología e innovaciónAsociación de Bananeros de ColombiaCorporación para Investigaciones BiológicasUniversidad Nacional de ColombiaEstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86241/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1110444301.20241.pdf1110444301.20241.pdfTesis de Doctorado en Biotecnologíaapplication/pdf3311900https://repositorio.unal.edu.co/bitstream/unal/86241/3/1110444301.20241.pdfa7a486e3216cddb32952a8e4cc18604dMD53THUMBNAIL1110444301.20241.pdf.jpg1110444301.20241.pdf.jpgGenerated Thumbnailimage/jpeg5417https://repositorio.unal.edu.co/bitstream/unal/86241/4/1110444301.20241.pdf.jpg5500f5ef84dd35cc8ed1a7c36c21b141MD54unal/86241oai:repositorio.unal.edu.co:unal/862412024-08-25 23:11:26.01Repositorio Institucional Universidad Nacional de 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

Análisis genómico y transcriptómico de la interacción musa - pseudocercospora fijiensis como herramienta en la identificación y caracterización de genes fúngicos de virulencia y patogenicidad

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