Caracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando Radseq
Ilustraciones, fotografías, tablas
- Autores:
-
Tarazona Pulido, Lina Maria
- Tipo de recurso:
- Fecha de publicación:
- 2024
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/86616
- Palabra clave:
- 570 - Biología::576 - Genética y evolución
Procesamiento de señales genómicas
Genomic signal processing
Marcador genético
Genetic markers
Variación genética
Genetic variation
Diversidad genética
Estructura poblacional
SNPs
PST1
Ómicas
Genetic diversity
Population structure
Omics
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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|
| dc.title.spa.fl_str_mv |
Caracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando Radseq |
| dc.title.translated.eng.fl_str_mv |
Genomic characterization of populations of Petiveria alliacea L. and Lippia alba (Mill.) from different departments of Colombia using RADseq |
| title |
Caracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando Radseq |
| spellingShingle |
Caracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando Radseq 570 - Biología::576 - Genética y evolución Procesamiento de señales genómicas Genomic signal processing Marcador genético Genetic markers Variación genética Genetic variation Diversidad genética Estructura poblacional SNPs PST1 Ómicas Genetic diversity Population structure Omics |
| title_short |
Caracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando Radseq |
| title_full |
Caracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando Radseq |
| title_fullStr |
Caracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando Radseq |
| title_full_unstemmed |
Caracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando Radseq |
| title_sort |
Caracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando Radseq |
| dc.creator.fl_str_mv |
Tarazona Pulido, Lina Maria |
| dc.contributor.advisor.none.fl_str_mv |
Rugeles Silva, Paula Andrea López Álvarez, Diana Carolina |
| dc.contributor.author.none.fl_str_mv |
Tarazona Pulido, Lina Maria |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Diversidad Biológica |
| dc.contributor.orcid.spa.fl_str_mv |
https://orcid.org/0009-0003-3606-1446 |
| dc.contributor.researchgate.spa.fl_str_mv |
https://www.researchgate.net/profile/Lina-Tarazona-Pulido |
| dc.contributor.googlescholar.spa.fl_str_mv |
https://scholar.google.com/citations?hl=es&user=J-X7K-AAAAAJ |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::576 - Genética y evolución |
| topic |
570 - Biología::576 - Genética y evolución Procesamiento de señales genómicas Genomic signal processing Marcador genético Genetic markers Variación genética Genetic variation Diversidad genética Estructura poblacional SNPs PST1 Ómicas Genetic diversity Population structure Omics |
| dc.subject.agrovoc.none.fl_str_mv |
Procesamiento de señales genómicas Genomic signal processing Marcador genético Genetic markers Variación genética Genetic variation |
| dc.subject.proposal.spa.fl_str_mv |
Diversidad genética Estructura poblacional SNPs PST1 Ómicas |
| dc.subject.proposal.eng.fl_str_mv |
Genetic diversity Population structure Omics |
| description |
Ilustraciones, fotografías, tablas |
| publishDate |
2024 |
| dc.date.accessioned.none.fl_str_mv |
2024-07-25T16:04:41Z |
| dc.date.available.none.fl_str_mv |
2024-07-25T16:04:41Z |
| dc.date.issued.none.fl_str_mv |
2024-03-15 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/86616 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/86616 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rugeles Silva, Paula Andrea2c2e97576e44a4f4259bc1916adc4552López Álvarez, Diana Carolina1ab298e9492bfd0c4b69bb243416b257Tarazona Pulido, Lina Maria69dfcf47e5b5075bd6f962d7ad7240a7Grupo de Investigación en Diversidad Biológicahttps://orcid.org/0009-0003-3606-1446https://www.researchgate.net/profile/Lina-Tarazona-Pulidohttps://scholar.google.com/citations?hl=es&user=J-X7K-AAAAAJ2024-07-25T16:04:41Z2024-07-25T16:04:41Z2024-03-15https://repositorio.unal.edu.co/handle/unal/86616Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Ilustraciones, fotografías, tablasLas investigaciones sobre la genética de plantas medicinales que pueden ser de interés por su potencial farmacológico son escazas, se realizó una revisión bibliográfica donde se destaca el uso de tecnologías Ómicas en la investigación de plantas medicinales en Colombia, y sobre la información existente de la etnobotánica, genómica, fitocompuestos y medicina tradicional que se les conoce. Las especies Lippia alba y Petiveria alliacea, son de interés para el programa GAT por el potencial anticancerígeno que pueden tener, por lo que es importante conocer su diversidad genética y estructura poblacional. Se colectaron muestras foliares de 31 individuos de P. alliacea y 17 de L. alba en diferentes regiones de Colombia. Se extrajo el ADN y se construyeron librerías RADseq utilizando la enzima de restricción PstI para su secuenciación, posteriormente se siguió un flujo bioinformático para la canalización de los archivos fasta obtenidos, que permitió identificar miles de polimorfismos de un solo nucleótido (SNPs) para cada una. Con los cuales se identificó que en las poblaciones de L. alba existe una variación genética entre poblaciones de 3.4%, con un FST de 0.062, mientras que P. alliacea tuvo un 75% de variación entre poblaciones y un FST de 0.747. Adicionalmente los valores de diversidad genética obtenidos incluyendo valores de Ho y He y el valor r de la prueba de mantel podrían permitir pensar que los diferentes modos reproductivos que tienen estas especies, así como el aprovechamiento antropogénico de estas influyeron en la variación genética y la estructura de la población obtenida. (Texto tomado de la fuente)Research on the genetics of medicinal plants, which may be of interest due to their pharmacological potential, is scarce. A literature review was conducted highlighting the use of Omics technologies in medicinal plant research in Colombia, along with existing information on ethnobotany, genomics, phytochemicals, and traditional medicine associated with these plants. The species Lippia alba and Petiveria alliacea are of interest to the GAT program due to their potential anticancer properties, making it important to understand their genetic diversity and population structure. Leaf samples were collected from 31 individuals of P. alliacea and 17 of L. alba in different regions of Colombia. DNA was extracted, and RADseq libraries were constructed using the restriction enzyme PstI for sequencing. Subsequently, a bioinformatics workflow was followed to process the obtained fasta files, allowing for the identification of thousands of single nucleotide polymorphisms (SNPs) for each species. It was identified that in L. alba populations, there is a genetic variation of 3.4% between populations, with an FST of 0.062, while P. alliacea exhibited a 75% variation between populations and an FST of 0.747. Additionally, the values of genetic diversity obtained, including Ho and He values, and the r value of the Mantel test, could suggest that the different reproductive modes of these species, as well as their anthropogenic exploitation, influenced the genetic variation and population structure observed.MaestríaMagíster en Ciencias BiológicasSe colectaron muestras foliares de 31 individuos de P. alliacea y 17 de L. alba en diferentes regiones de Colombia. Se extrajo el ADN y se construyeron librerías RADseq utilizando la enzima de restricción PstI para su secuenciación, posteriormente se siguió un flujo bioinformático para la canalización de los archivos fasta obtenidos, que permitió identificar miles de polimorfismos de un solo nucleótido (SNPs) para cada una.Ciencias Agropecuarias.Sede Palmiraxi, 112 páginas + anexosapplication/pdfspaUniversidad Nacional de ColombiaPalmira - Ciencias Agropecuarias - Maestría en Ciencias BiológicasFacultad de Ciencias AgropecuariasPalmira, Valle del Cauca, ColombiaUniversidad Nacional de Colombia - Sede Palmira570 - Biología::576 - Genética y evoluciónProcesamiento de señales genómicasGenomic signal processingMarcador genéticoGenetic markersVariación genéticaGenetic variationDiversidad genéticaEstructura poblacionalSNPsPST1ÓmicasGenetic diversityPopulation structureOmicsCaracterización genómica de las poblaciones de Petiveria alliacea L. y Lippia alba (Mill.) de diferentes departamentos de Colombia utilizando RadseqGenomic characterization of populations of Petiveria alliacea L. and Lippia alba (Mill.) from different departments of Colombia using RADseqTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbasi, F., Abdel-Massih, R. 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Food Research International, 130(July 2019), 108938. https://doi.org/10.1016/j.foodres.2019.108938Bartolome, A. P., Villaseñor, I. M., & Yang, W. C. (2013). Bidens pilosa L. (Asteraceae): Botanical Properties, Traditional Uses, Phytochemistry, and Pharmacology. Evidence- based Complementary and Alternative Medicine : eCAM, 2013, 51. https://doi.org/10.1155/2013/340215Bernal, H. (2011). Pautas para el conocimiento, conservación y uso sostenible de plantas medicinales nativas en Colombia.Bernal, R., Galeano, G., Rodríguez, A., Sarmiento, H., & Gutiérrez, M. (2017). Nombres Comunes Plantas de Colombia. http://www.biovirtual.unal.edu.co/nombrescomunes/Bernal, R., Gradstein, S. R., & Celis, M. (2019). Catálogo de plantas y líquenes de Colombia. Instituto de Ciencias Naturales. Universidad Nacional de Colombia. http://catalogoplantasdecolombia.unal.edu.coBoutanaev, A. M., Moses, T., Zi, J., Nelson, D. R., Mugford, S. T., Peters, R. J., & Osbourn, A. (2014). 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Journal of Biotechnology, 166(3), 122-134. https://doi.org/10.1016/J.JBIOTEC.2013.04.004Aproximación biológica, fitoquímica y agronómica para la gestión de recursos vegetales con potencial farmacológico: Aporte a cadena de valor para el sector de los fitomedicamentos en ColombiaPontificia Universidad JaverianaEstudiantesInvestigadoresPersonal de apoyo escolarProveedores de ayuda financiera para estudiantesPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86616/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1070970724.2024.pdf1070970724.2024.pdfTesis de Maestría en Ciencias Biológicasapplication/pdf4716641https://repositorio.unal.edu.co/bitstream/unal/86616/2/1070970724.2024.pdf6bc3619e872960dd6399da255f7e157fMD52THUMBNAIL1070970724.2024.pdf.jpg1070970724.2024.pdf.jpgGenerated 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