Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis

In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative...

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Autores:
Gallego Rúa, Adriana María
Henao Ramírez, Ana María
Urrea Trujillo, Aura Inés
Atehortúa Garcés, Lucía
Tipo de recurso:
Article of journal
Fecha de publicación:
2016
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/61196
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/61196
http://bdigital.unal.edu.co/60004/
Palabra clave:
57 Ciencias de la vida; Biología / Life sciences; biology
antioxidants
histology
reserve accumulation
recalcitrance
somatic embryogenesis
acumulación de reservas
antioxidantes
embriogénesis somática
histología
recalcitrancia.
acumulación de reservas
antioxidantes
embriogénesis somática
histología
recalcitrancia
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_6e15c806743cb1eb8a835b5908ffbe9a
oai_identifier_str oai:repositorio.unal.edu.co:unal/61196
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis
title Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis
spellingShingle Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis
57 Ciencias de la vida; Biología / Life sciences; biology
antioxidants
histology
reserve accumulation
recalcitrance
somatic embryogenesis
acumulación de reservas
antioxidantes
embriogénesis somática
histología
recalcitrancia.
acumulación de reservas
antioxidantes
embriogénesis somática
histología
recalcitrancia
title_short Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis
title_full Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis
title_fullStr Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis
title_full_unstemmed Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis
title_sort Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis
dc.creator.fl_str_mv Gallego Rúa, Adriana María
Henao Ramírez, Ana María
Urrea Trujillo, Aura Inés
Atehortúa Garcés, Lucía
dc.contributor.author.spa.fl_str_mv Gallego Rúa, Adriana María
Henao Ramírez, Ana María
Urrea Trujillo, Aura Inés
Atehortúa Garcés, Lucía
dc.subject.ddc.spa.fl_str_mv 57 Ciencias de la vida; Biología / Life sciences; biology
topic 57 Ciencias de la vida; Biología / Life sciences; biology
antioxidants
histology
reserve accumulation
recalcitrance
somatic embryogenesis
acumulación de reservas
antioxidantes
embriogénesis somática
histología
recalcitrancia.
acumulación de reservas
antioxidantes
embriogénesis somática
histología
recalcitrancia
dc.subject.proposal.spa.fl_str_mv antioxidants
histology
reserve accumulation
recalcitrance
somatic embryogenesis
acumulación de reservas
antioxidantes
embriogénesis somática
histología
recalcitrancia.
acumulación de reservas
antioxidantes
embriogénesis somática
histología
recalcitrancia
description In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating variety had a high presence of polysaccharides with random distribution of polyphenols at the end of the embryo induction step.
publishDate 2016
dc.date.issued.spa.fl_str_mv 2016-05-01
dc.date.accessioned.spa.fl_str_mv 2019-07-02T20:41:09Z
dc.date.available.spa.fl_str_mv 2019-07-02T20:41:09Z
dc.type.spa.fl_str_mv Artículo de revista
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dc.identifier.issn.spa.fl_str_mv ISSN: 1900-1649
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/61196
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/60004/
identifier_str_mv ISSN: 1900-1649
url https://repositorio.unal.edu.co/handle/unal/61196
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dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/actabiol/article/view/50196
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Acta Biológica Colombiana
Acta Biológica Colombiana
dc.relation.references.spa.fl_str_mv Gallego Rúa, Adriana María and Henao Ramírez, Ana María and Urrea Trujillo, Aura Inés and Atehortúa Garcés, Lucía (2016) Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis. Acta Biológica Colombiana, 21 (2). pp. 335-345. ISSN 1900-1649
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá - Faculdad de Ciencias - Departamento de Biología
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/61196/1/50196-289621-1-PB.pdf
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repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gallego Rúa, Adriana María5dfb2c40-3a22-4b8e-b734-8891288faa45300Henao Ramírez, Ana María56d3629b-5c45-4fc9-8c60-3cae2f4a6574300Urrea Trujillo, Aura Inésb8f322a2-3b79-4cc8-b29e-7844cfb135f6300Atehortúa Garcés, Lucía3e80e4c3-f88b-476d-b1a1-45350e9b1a513002019-07-02T20:41:09Z2019-07-02T20:41:09Z2016-05-01ISSN: 1900-1649https://repositorio.unal.edu.co/handle/unal/61196http://bdigital.unal.edu.co/60004/In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating variety had a high presence of polysaccharides with random distribution of polyphenols at the end of the embryo induction step.Dos variedades de cacao con diferentes respuestas a la regeneración fueron descritas en función de su capacidad para almacenar diferentes compuestos, con el fin de aproximarse al entendimiento de las causas de la falta de regeneración en embriones somáticos de cacao. Es bien sabido que las reservas de semillas desempeñan un papel central en la capacidad de regeneración de embriones somáticos; por tanto, se realizó un seguimiento de cambios histoquímicos y fluctuaciones de reserva de proteínas, polisacáridos y polifenoles durante la embriogénesis somática (SE) en dos variedades de cacao. El estudio mostró que, en los embriones somáticos de la variedad regenerante, los polifenoles se localizaron principalmente en la periferia del embrión (células de la epidermis) y las proteínas fueron el componente principal de almacenamiento en el medio de expresión de embriones, mientras que la variedad no regenerante tenía una alta presencia de polisacáridos y una distribución aleatoria de los polifenoles en el final de la etapa de inducción de embriones.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Faculdad de Ciencias - Departamento de Biologíahttps://revistas.unal.edu.co/index.php/actabiol/article/view/50196Universidad Nacional de Colombia Revistas electrónicas UN Acta Biológica ColombianaActa Biológica ColombianaGallego Rúa, Adriana María and Henao Ramírez, Ana María and Urrea Trujillo, Aura Inés and Atehortúa Garcés, Lucía (2016) Polyphenols distributions and reserve substances analysis in cacao somatic embryogenesis. Acta Biológica Colombiana, 21 (2). pp. 335-345. ISSN 1900-164957 Ciencias de la vida; Biología / Life sciences; biologyantioxidantshistologyreserve accumulationrecalcitrancesomatic embryogenesisacumulación de reservasantioxidantesembriogénesis somáticahistologíarecalcitrancia.acumulación de reservasantioxidantesembriogénesis somáticahistologíarecalcitranciaPolyphenols distributions and reserve substances analysis in cacao somatic embryogenesisArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL50196-289621-1-PB.pdfapplication/pdf7687825https://repositorio.unal.edu.co/bitstream/unal/61196/1/50196-289621-1-PB.pdff649b0ff0a5a1c92b8f9affe4d314a7fMD51THUMBNAIL50196-289621-1-PB.pdf.jpg50196-289621-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8575https://repositorio.unal.edu.co/bitstream/unal/61196/2/50196-289621-1-PB.pdf.jpg0caa54662f799dfc64fc18f164239984MD52unal/61196oai:repositorio.unal.edu.co:unal/611962024-04-17 23:50:11.866Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

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