Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide

ilustraciones, graficas

Autores:
Castillo Perdomo, Diana Marcela
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/83320
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/83320
https://repositorio.unal.edu.co
Palabra clave:
610 - Medicina y salud::616 - Enfermedades
Cromatografía
Artritis Reumatoide
Arthritis, Rheumatoid
Chromatography
Porphyromonas gingivalis
RgpA
PPAD
Gingipaínas
Artritis Reumatoidea
Periodontitis
Gingipains
Rheumatoid arthritis
Rights
openAccess
License
Atribución-NoComercial-SinDerivadas 4.0 Internacional
id UNACIONAL2_6dad15ca02c4721a95078d9f853c9932
oai_identifier_str oai:repositorio.unal.edu.co:unal/83320
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide
dc.title.translated.eng.fl_str_mv Presence of RgpA, anti-RgpA antibodies and their association with rheumatoid arthritis
title Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide
spellingShingle Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide
610 - Medicina y salud::616 - Enfermedades
Cromatografía
Artritis Reumatoide
Arthritis, Rheumatoid
Chromatography
Porphyromonas gingivalis
RgpA
PPAD
Gingipaínas
Artritis Reumatoidea
Periodontitis
Gingipains
Rheumatoid arthritis
title_short Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide
title_full Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide
title_fullStr Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide
title_full_unstemmed Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide
title_sort Presencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoide
dc.creator.fl_str_mv Castillo Perdomo, Diana Marcela
dc.contributor.advisor.none.fl_str_mv Castellanos Parra, Jaime Eduardo
Romero Sánchez, María Consuelo
dc.contributor.author.none.fl_str_mv Castillo Perdomo, Diana Marcela
dc.contributor.researchgroup.spa.fl_str_mv Unidad de Investigación Básica Oral - UIBO
dc.contributor.subjectmatterexpert.none.fl_str_mv Lafaurie Villamil Gloria Inés
dc.contributor.orcid.spa.fl_str_mv Castillo, Diana Marcela [0000-0002-1846-7448]
dc.contributor.cvlac.spa.fl_str_mv Castillo Perdomo, Diana Marcela [https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000293580]
dc.contributor.scopus.spa.fl_str_mv Castillo, Diana Marcela [16300829800]
dc.contributor.researchgate.spa.fl_str_mv Castillo, Diana Marcela [https://www.researchgate.net/profile/Diana-Castillo-4]
dc.contributor.googlescholar.spa.fl_str_mv Castillo, Diana Marcela [https://scholar.google.com/citations?user=_DyKomkAAAAJ&hl=es]
dc.subject.ddc.spa.fl_str_mv 610 - Medicina y salud::616 - Enfermedades
topic 610 - Medicina y salud::616 - Enfermedades
Cromatografía
Artritis Reumatoide
Arthritis, Rheumatoid
Chromatography
Porphyromonas gingivalis
RgpA
PPAD
Gingipaínas
Artritis Reumatoidea
Periodontitis
Gingipains
Rheumatoid arthritis
dc.subject.other.spa.fl_str_mv Cromatografía
Artritis Reumatoide
dc.subject.other.eng.fl_str_mv Arthritis, Rheumatoid
Chromatography
dc.subject.proposal.none.fl_str_mv Porphyromonas gingivalis
RgpA
PPAD
dc.subject.proposal.spa.fl_str_mv Gingipaínas
Artritis Reumatoidea
Periodontitis
dc.subject.proposal.eng.fl_str_mv Gingipains
Rheumatoid arthritis
description ilustraciones, graficas
publishDate 2022
dc.date.issued.none.fl_str_mv 2022-12-02
dc.date.accessioned.none.fl_str_mv 2023-02-06T16:52:57Z
dc.date.available.none.fl_str_mv 2023-02-06T16:52:57Z
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TD
format http://purl.org/coar/resource_type/c_db06
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/83320
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co
url https://repositorio.unal.edu.co/handle/unal/83320
https://repositorio.unal.edu.co
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
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dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
institution Universidad Nacional de Colombia
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spelling Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Castellanos Parra, Jaime Eduardod005422b5147ff1af3f046ffaf4328e6Romero Sánchez, María Consueloadef63ccda8465428682bb4b85aab02bCastillo Perdomo, Diana Marcela290a242638aa37c84584c196fdce5fc0Unidad de Investigación Básica Oral - UIBOLafaurie Villamil Gloria InésCastillo, Diana Marcela [0000-0002-1846-7448]Castillo Perdomo, Diana Marcela [https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000293580]Castillo, Diana Marcela [16300829800]Castillo, Diana Marcela [https://www.researchgate.net/profile/Diana-Castillo-4]Castillo, Diana Marcela [https://scholar.google.com/citations?user=_DyKomkAAAAJ&hl=es]2023-02-06T16:52:57Z2023-02-06T16:52:57Z2022-12-02https://repositorio.unal.edu.co/handle/unal/83320Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.coilustraciones, graficasEstudios clínicos y epidemiológicos han corroborado la asociación bidireccional entre la artritis reumatoide (AR) y la periodontitis. Esta asociación es atribuida a Porphyromonas gingivalis, por la presencia de la enzima peptidil arginina deiminasa (PPAD) y con la actividad de las Arg-gingipaínas en la citrulinación, vinculadas con el quiebre de la tolerancia inmunológica en la AR. Se ha confirmado que los anticuerpos anti-P. gingivalis acompañan el desarrollo de la AR. Sin embargo, pocos estudios indagan la asociación de factores de virulencia específicos de esta bacteria con la AR. En este estudio hemos producido la PPAD de manera recombinante y purificado la gingipaína RgpA nativa a partir de vesículas de membrana externa de P. gingivalis ATCC33277, la actividad enzimática se evaluó con sustratos fluorogénicos. La presencia de las proteínas se confirmó por SDS-PAGE y Western Blot y fueron usadas como antígenos en la detección de los anticuerpos contra estos factores de virulencia mediante ELISA en 143 pacientes con AR y 112 individuos sin AR. Se encontraron diferencias significativas en los títulos de anti-RgpA entre los dos grupos y se determinó asociación con el diagnóstico de AR (OR 4.09; IC 95% 1.2-13.9), mientras que los anti-PPAD no presentan diferencias. La interacción anti-RgpA/anti-PPAD se asoció con diagnósticos de AR (OR 6.63; IC 95% 1.61-27). Además, se purificaron con éxito los dos factores de virulencia de P. gingivalis y se desarrolló el ELISA, permitiendo la detección de los anticuerpos frente a estas proteínas. Los anticuerpos anti-RgpA y la doble positividad de los anticuerpos anti-RgpA/anti-PPAD pueden ser marcadores diagnósticos de la AR. (Texto tomado de la fuente)Clinical and epidemiological studies have confirmed the bidirectional association between rheumatoid arthritis (RA) and periodontitis. This association is attributed to Porphyromonas gingivalis, due to the presence of peptidyl arginine deiminase (PPAD) together with the action of Arg-gingipains in citrullination, linked to the alteration of immunological tolerance in RA. It has been confirmed that anti-P. gingivalis accompany the development of RA. Nevertheless. Few studies investigate the association of specific virulence factors of this bacterium with RA. In this study we have recombinantly produced PPAD and purified native RgpA gingipain from outer membrane vesicles of P. gingivalis ATCC33277, the enzymatic activity was evaluated with fluorogenic substrates. The presence of the proteins was confirmed by SDS-PAGE and Western Blot and they were used as antigens in the detection of antibodies against these virulence factors by ELISA in 143 patients with RA and 112 individuals without RA. Significant differences were found in anti-RgpA titers between the two groups and an association with RA was determined (OR 4.09; 95% CI 1.2-13.9), while anti-PPAD did not present differences. The anti-RgpA/anti-PPAD interaction was associated with RA diagnoses (OR 6.63; 95% CI 1.61-27). In addition, the two virulence factors of P. gingivalis were successfully purified and the ELISA was developed, allowing the detection of antibodies against these proteins. Anti-RgpA antibodies and the double positivity of anti-RgpA/anti-PPAD antibodies can be diagnostic markers of RA.Minciencias- Ministerio de Ciencia Tecnología e Innovación. Contrato: 642-2017 Código: 130874455642DoctoradoDoctor en BiotecnologíaMicrobiología Oralxxi, 96 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Doctorado en BiotecnologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::616 - EnfermedadesCromatografíaArtritis ReumatoideArthritis, RheumatoidChromatographyPorphyromonas gingivalisRgpAPPADGingipaínasArtritis ReumatoideaPeriodontitisGingipainsRheumatoid arthritisPresencia de RgpA, anticuerpos anti-RgpA y su asociación con artritis reumatoidePresence of RgpA, anti-RgpA antibodies and their association with rheumatoid arthritisTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAdams, B., Nunes, J. M., Page, M. J., Roberts, T., Carr, J., Nell, T. A., Kell, D. B., & Pretorius, E. (2019). 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Molecular microbiology, 89(5), 903–917. https://doi.org/10.1111/mmi.12319Minciencias- Ministerio de Ciencia Tecnología e Innovación.InvestigadoresORIGINAL52882038.2022.pdf52882038.2022.pdfTesis Doctorado en Biotecnologíaapplication/pdf3282943https://repositorio.unal.edu.co/bitstream/unal/83320/2/52882038.2022.pdf41c0e5a5703d2a928fbb2f6db41ff54cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/83320/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51THUMBNAIL52882038.2022.pdf.jpg52882038.2022.pdf.jpgGenerated Thumbnailimage/jpeg4912https://repositorio.unal.edu.co/bitstream/unal/83320/3/52882038.2022.pdf.jpg86793533fb9a8dc4d6776d237f7d2d21MD53unal/83320oai:repositorio.unal.edu.co:unal/833202024-08-17 23:12:56.405Repositorio Institucional Universidad Nacional de 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