Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi

ilustraciones, diagramas

Autores:: Pérez Mora, Walter Hernando

Tipo de recurso:: Doctoral thesis

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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dc.title.spa.fl_str_mv	Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi
dc.title.translated.eng.fl_str_mv	Metabolomics and proteomics-based approach for studying mechanisms associated with resistance induction through the use of commercial inducing substances in carnation (Dianthus caryophyllus L) for the control of vascular wilt caused by Fusarium oxysporum f. sp. dianthi
title	Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi
spellingShingle	Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi 540 - Química y ciencias afines 570 - Biología::572 - Bioquímica 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Floricultura Protección de las plantas Vitamina B1 Floriculture Plants, protection of Vitamin B1 Clavel Inductor de resistencia Sensibilización inmunidad innata en plantas Tiamina Resistance inductors Carnation Plant innate immunity Priming Resistance inductors Thiamine
title_short	Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi
title_full	Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi
title_fullStr	Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi
title_full_unstemmed	Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi
title_sort	Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi
dc.creator.fl_str_mv	Pérez Mora, Walter Hernando
dc.contributor.advisor.none.fl_str_mv	Melgarejo Muñoz, Luz Marina Ardila Barrantes, Harold Duban
dc.contributor.author.none.fl_str_mv	Pérez Mora, Walter Hernando
dc.contributor.researchgroup.spa.fl_str_mv	Fisiología del Estrés y Biodiversidad en Plantas y Microorganismos Estudio de Actividades Metabólicas Vegetales
dc.contributor.orcid.spa.fl_str_mv	Pérez Mora, Walter Hernando [0000000272901874]
dc.contributor.cvlac.spa.fl_str_mv	Pérez Mora, Walter Hernando [0001375535]
dc.contributor.researchgate.spa.fl_str_mv	Pérez Mora, Walter Hernando [https://www.researchgate.net/profile/Walter-Perez-Mora]
dc.contributor.googlescholar.spa.fl_str_mv	Pérez Mora, Walter Hernando [https://scholar.google.com/citations?user=hKtonb0AAAAJ&hl=es]
dc.subject.ddc.spa.fl_str_mv	540 - Química y ciencias afines 570 - Biología::572 - Bioquímica 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
topic	540 - Química y ciencias afines 570 - Biología::572 - Bioquímica 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Floricultura Protección de las plantas Vitamina B1 Floriculture Plants, protection of Vitamin B1 Clavel Inductor de resistencia Sensibilización inmunidad innata en plantas Tiamina Resistance inductors Carnation Plant innate immunity Priming Resistance inductors Thiamine
dc.subject.lemb.spa.fl_str_mv	Floricultura Protección de las plantas Vitamina B1
dc.subject.lemb.eng.fl_str_mv	Floriculture Plants, protection of Vitamin B1
dc.subject.proposal.spa.fl_str_mv	Clavel Inductor de resistencia Sensibilización inmunidad innata en plantas Tiamina
dc.subject.proposal.eng.fl_str_mv	Resistance inductors Carnation Plant innate immunity Priming Resistance inductors Thiamine
description	ilustraciones, diagramas
publishDate	2023
dc.date.issued.none.fl_str_mv	2023
dc.date.accessioned.none.fl_str_mv	2024-01-18T20:05:54Z
dc.date.available.none.fl_str_mv	2024-01-18T20:05:54Z
dc.type.spa.fl_str_mv	Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TD
format	http://purl.org/coar/resource_type/c_db06
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/85369
url	https://repositorio.unal.edu.co/handle/unal/85369
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.format.extent.spa.fl_str_mv	xxi, 179 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Doctorado en Ciencias - Química
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Melgarejo Muñoz, Luz Marinac13b62e709cbe2df09a40b59645414d3Ardila Barrantes, Harold Duban3c0b4dc91cdf02792559faddcd37e2b3Pérez Mora, Walter Hernando5fe7de3b492010d6563eb85a847f1d3fFisiología del Estrés y Biodiversidad en Plantas y MicroorganismosEstudio de Actividades Metabólicas VegetalesPérez Mora, Walter Hernando [0000000272901874]Pérez Mora, Walter Hernando [0001375535]Pérez Mora, Walter Hernando [https://www.researchgate.net/profile/Walter-Perez-Mora]Pérez Mora, Walter Hernando [https://scholar.google.com/citations?user=hKtonb0AAAAJ&hl=es]2024-01-18T20:05:54Z2024-01-18T20:05:54Z2023https://repositorio.unal.edu.co/handle/unal/85369ilustraciones, diagramasLa floricultura es un sector económico de interés en Colombia, siendo el clavel uno de los productos destacados. No obstante, el clavel se ve afectado por enfermedades como el marchitamiento vascular causado por Fusarium oxysporum f.sp. dianthi (Fod), el cual es el factor de mayor impacto en la producción. Ante esta problemática, se están explorando distintas estrategias para el control de esta enfermedad, como es el caso de la potencialización de la respuesta de defensa innata mediante el uso de inductores de resistencia. En el presente estudio se evaluó el efecto que tienen moléculas inductoras en el patosistema y se seleccionó la tiamina aplicada por aspersión foliar en concentración 1mmol L-1 como potencial inductor de resistencia para el control del patógeno en el clavel. Posteriormente, se estudiaron los cambios bioquímicos causados por la tiamina en la planta a nivel de proteínas y metabolitos, usando herramientas de la proteómica y la metabólomica. La aplicación de tiamina promueve la acumulación de proteínas de diversos procesos bioquímicos en la raíz, que han sido previamente vinculados a la defensa vegetal. Además, también promueve la acumulación de metabolitos de tipo flavonoide y derivados del antranilato, que han sido relacionados a cultivares de clavel resistentes a Fod. Los resultados obtenidos sugieren que los tratamientos con tiamina permiten la activación de mecanismos de defensa que protegen a la planta frente al patógeno. Estos resultados aumentan el conocimiento sobre el uso de tiamina como agente bioestimulante en plantas y permiten proponer los principales mecanismos bioquímicos asociados a su modo de acción. (Texto tomado de la fuente)Floriculture holds significant economic importance in Colombia, with carnation among the prominent products. Unfortunately, carnations are highly susceptible to diseases, with vascular wilt caused by Fusarium oxysporum f. sp. dianthi (Fod) being a major production-limiting factor. Various strategies are being explored to address this issue, including enhancing the innate defense response using resistance inducers. In this study, we investigated the effect of inducing molecules on the pathosystem and specifically evaluated thiamine as a potential resistance inducer for controlling the Fod pathogen incarnation. Thiamine was applied through foliar spraying at a concentration of 1mmol L-1. Subsequently, we delved into the biochemical changes induced by thiamine in the plants at the protein and metabolite levels, employing proteomic and metabolomic tools. Remarkably, thiamine application promoted the accumulation of proteins involved in diverse biochemical processes in the roots, which have been previously linked to plant defense mechanisms. Additionally, it stimulated the accumulation of flavonoid-type metabolites and anthranilate derivatives, known to be associated with Fod-resistant carnation cultivars. These results suggest that thiamine treatments activate defense mechanisms safeguarding plants against this pathogen. By shedding light on using thiamine as a biostimulant agent in plants, our findings contribute to a better understanding of its mode of action. This knowledge allows us to propose the primary biochemical mechanisms associated with thiamine effectiveness in bolstering plant resistance. Overall, our study opens new possibilities for disease control in carnation and advances our comprehension of how thiamine can be harnessed as a valuable tool in promoting plant defense strategies.DoctoradoDoctor en Ciencias - QuímicaBioquímica de las interacciones hospedero-patógenoxxi, 179 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Doctorado en Ciencias - QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá540 - Química y ciencias afines570 - Biología::572 - Bioquímica630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materialesFloriculturaProtección de las plantasVitamina B1FloriculturePlants, protection ofVitamin B1ClavelInductor de resistenciaSensibilizacióninmunidad innata en plantasTiaminaResistance inductorsCarnationPlant innate immunityPrimingResistance inductorsThiamineAproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthiMetabolomics and proteomics-based approach for studying mechanisms associated with resistance induction through the use of commercial inducing substances in carnation (Dianthus caryophyllus L) for the control of vascular wilt caused by Fusarium oxysporum f. sp. dianthiTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDAbd-elsalam, K. 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Environmental and Experimental Botany, 85, 22–29. https://doi.org/10.1016/j.envexpbot.2012.07.008Estudio de las respuestas bioquímicas, moleculares y fisiológicas ocasionadas por la aplicación de inductores de resistencia en el clavel (Dianthus caryophyllus L): nuevas alternativas limpias para el control del marchitamiento vascular, con número de contrato RC No. 80740-148-2019Ministerio de Ciencia, Tecnología e InnovaciónUniversidad Nacional de ColombiaBibliotecariosEstudiantesInvestigadoresMaestrosProveedores de ayuda financiera para estudiantesPúblico generalReceptores de fondos federales y solicitantesLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85369/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1013576890.2023.pdf1013576890.2023.pdfTesis de Doctorado en Ciencias - 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Aproximación metabolómica y proteómica para el estudio de los mecanismos asociados a la inducción de resistencia mediante el uso de sustancias inductoras comerciales en clavel (Dianthus caryophyllus L), para el control del marchitamiento vascular causado por Fusarium oxysporum f. sp. dianthi

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