Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)

ilustraciones (principalmente a color), diagramas, fotografías

Autores:: Hernández Jirón, Isabel Cristina

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)
dc.title.translated.eng.fl_str_mv	Evaluation of a transformation vector in p. fluorescens ibun S1602 for production of a short chain (PHAscl) and medium chain (PHAmcl) polyhydroxyalcanoates (PHAs) copolymer
title	Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)
spellingShingle	Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl) 570 - Biología::572 - Bioquímica Polihidroxialcanoatos Biopolímeros Poliésteres Vectores Genéticos Pseudomonas fluorescens Burkholderia Polyhydroxyalkanoates Biopolymers Polyesters Genetic Vectors Polyhydroxyalkanoates PHAs copolymer Burkholderia Pseudomonas short and medium chain Polihidroxialcanoatos PHAs copolímero Burkholderia Pseudomonas Cadena corta y media
title_short	Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)
title_full	Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)
title_fullStr	Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)
title_full_unstemmed	Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)
title_sort	Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)
dc.creator.fl_str_mv	Hernández Jirón, Isabel Cristina
dc.contributor.advisor.spa.fl_str_mv	Bernal Morales, José Mauricio Moreno Sarmiento, Nubia
dc.contributor.author.spa.fl_str_mv	Hernández Jirón, Isabel Cristina
dc.contributor.researchgroup.spa.fl_str_mv	Bioprocesos y Bioprospección
dc.subject.ddc.spa.fl_str_mv	570 - Biología::572 - Bioquímica
topic	570 - Biología::572 - Bioquímica Polihidroxialcanoatos Biopolímeros Poliésteres Vectores Genéticos Pseudomonas fluorescens Burkholderia Polyhydroxyalkanoates Biopolymers Polyesters Genetic Vectors Polyhydroxyalkanoates PHAs copolymer Burkholderia Pseudomonas short and medium chain Polihidroxialcanoatos PHAs copolímero Burkholderia Pseudomonas Cadena corta y media
dc.subject.decs.spa.fl_str_mv	Polihidroxialcanoatos Biopolímeros Poliésteres Vectores Genéticos Pseudomonas fluorescens Burkholderia
dc.subject.decs.eng.fl_str_mv	Polyhydroxyalkanoates Biopolymers Polyesters Genetic Vectors
dc.subject.proposal.eng.fl_str_mv	Polyhydroxyalkanoates PHAs copolymer Burkholderia Pseudomonas short and medium chain
dc.subject.proposal.spa.fl_str_mv	Polihidroxialcanoatos PHAs copolímero Burkholderia Pseudomonas Cadena corta y media
description	ilustraciones (principalmente a color), diagramas, fotografías
publishDate	2023
dc.date.issued.none.fl_str_mv	2023
dc.date.accessioned.none.fl_str_mv	2024-01-15T17:02:40Z
dc.date.available.none.fl_str_mv	2024-01-15T17:02:40Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.spa.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/85280
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/85280 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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DETERMINACIÓN DEL CLUSTER (phaC1, phaZ, phaC2, phaD, phaF, phaI) ASOCIADO CON LA PRODUCCIÓN DE POLIHIDROXIALCANOATOS (PHAs) SINTASA TIPO II EN UNA CEPA NATIVA COLOMBIANA. Universidad Nacional de Colombia Serrano Riaño, J., Sastoque Rivera, L. Á., Castaño, D. M., & Moreno Sarmiento, N. (2011). Análisis de las polihidroxialcanoato sintasas (PhaC1 y PhaC2) en una cepa de Pseudomonas fluorescens IBUN S1602, aislada en suelos colombianos. Revista Colombiana de Biotecnología., XIII(2), 84–96 Slater, S. C., Voige, W. H., & Dennis, D. E. (1988). Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta-hydroxybutyrate biosynthetic pathway. Journal of Bacteriology, 170(10), 4431–4436. https://doi.org/10.1128/jb.170.10.4431-4436.1988 Tan, D., Yin, J., & Chen, G. Q. (2016). Production of Polyhydroxyalkanoates. 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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
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spelling	Atribución-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Bernal Morales, José Mauricio1e1d30ae7d64c3851743e2a4c7d40fd2Moreno Sarmiento, Nubia16311b987fedc341cbdf0f51389c4aebHernández Jirón, Isabel Cristinad7965e6eb76afb7749881aaf39e5f941Bioprocesos y Bioprospección2024-01-15T17:02:40Z2024-01-15T17:02:40Z2023https://repositorio.unal.edu.co/handle/unal/85280Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones (principalmente a color), diagramas, fotografíasLos Polihidroxialcanoatos (PHAs) son poliésteres acumulados intracelularmente como fuente de carbono y energía por bacterias en ciertas condiciones. Las características físicas y mecánicas del PHA depende del microorganismo usado, las proteínas, genes involucrados, las condiciones de crecimiento, la ruta metabólica y la fuente de carbono suministrada. Por ejemplo, los copolímeros de cadena corta y media exhiben características como, una temperatura de fusión más baja, un módulo de Young más bajo y un alargamiento de rotura más alto, esto significa que es un plástico más resistente y flexible. El Grupo de Investigación de Bioprocesos y Bioprospección de la Universidad Nacional (UNAL) ha enfocado sus esfuerzos en el estudio de microorganismos con potencial biotecnológico para establecer un aprovechamiento sostenible y rentable de bio-productos de interés comercial. Pseudomonas fluorescens y Burkholderia acumulan PHAs de cadena media (PHAmcl) y cadena corta (PHAscl) respectivamente, a partir de diversas fuentes de carbono y han sido objeto de estudio en el grupo de investigación. Análisis genéticos realizados por el grupo han demostrado que la especie P. fluorescens IBUN S1602 contiene la polimerasa tipo II para producción de PHAmcl, no obstante, hasta la fecha el grupo no ha hecho un reporte parecido para Burkholderia sp. IBUN 2G57. Dentro del trabajo realizado con IBUN S1602 se recomendó para futuras investigaciones establecer las condiciones de fermentación para producir PHAs e iniciar la manipulación genética usando el conocimiento de los genes involucrados en la producción de PHAmcl. El objetivo de este trabajo es aprovechar los recursos genéticos de Pseudomonas y Burkholderia para producir PHAs de cadena corta y media que podrían ser considerados más competitivos en el mercado y que suplieran las necesidades y limitaciones de la obtención de estos biopolímeros. En el desarrollo del proyecto se estandarizaron las condiciones de PCR de cinco pares de cebadores para amplificar el operón phaCAB y los genes phaC, phaA, phaB de Burkholderia sp. IBUN 2G57 involucrados en la producción de PHB (poli-3-Hidroxibutirato), se determinó que esta cepa tiene la organización genética de la sintasa tipo I al igual que la cepa más estudiada Cupriavidus necator, a su vez, se diseñó un vector de transformación con el operón phaCAB y el gen phaC y se realizaron ensayos de transformación de P. fluorescens IBUN S1602. La evaluación del vector de transformación construido en este trabajo llevó a considerar que los insertos provenientes de las cepas de Burkholderia presentaban un grado de toxicidad que impedían la exitosa transformación de IBUN S1602. (Texto tomado de la fuente)Polyhydroxyalkanoates (PHAs) are polyesters accumulated intracellularly as a carbon and energy source by bacteria under certain conditions. The physical and mechanical characteristics of PHA depends on the microorganism used, the proteins, genes involved, the growth conditions, the metabolic pathway and the carbon source supplied. For example, short and medium chain copolymers exhibit characteristics such as, lower melting temperature, lower Young's modulus, and higher elongation at break, this means that it is a stronger and more flexible plastic, leading to improved bioplastic properties. The Bioprocesses and Bioprospecting Research Group of the National University (UNAL) has focused its efforts on the study of microorganisms with biotechnological potential with the objective of establishing a sustainable and profitable use of bio-products of commercial interest. Pseudomonas fluorescens and Burkholderia are microorganisms that accumulate medium-chain PHAs (PHAmcl) and short-chain PHAs (PHAscl) respectively, using diverse carbon sources and have been the object of study in the research group. Genetic analyses performed by the group have shown that P. fluorescens IBUN S1602 contains the type II polymerase for PHAmcl production, however, to date the group has not made a similar report for Burkholderia sp. IBUN 2G57. Within the work carried out with IBUN S1602, it was recommended for future research to establish fermentation conditions for this strain and to initiate genetic manipulation taking advantage of the knowledge of the genes involved in PHAmcl production. The objective of this work is to take advantage of the genetic resources of Pseudomonas and Burkholderia to produce short- and medium-chain PHAs that could be considered more competitive in the market and that would meet the needs and limitations of obtaining these biopolymers. In the development of the project, the PCR conditions of five pairs of primers were optimized to amplify the pha operon and the phaC, phaA, phaB genes of Burkholderia sp. IBUN 2G57 involved in the production of PHB (poly-3-Hydroxybutyrate), it was determined that this strain has the genetic organization of type I synthase like the most studied strain Cupriavidus necator, in turn, a transformation vector with the pha operon and phaC gene was designed and transformation assays of P. fluorescens IBUN S1602 were performed. The evaluation of the transformation vector constructed in this work led to consider that the inserts from the Burkholderia strains presented a degree of toxicity that prevented the successful transformation of IBUN S1602.MaestríaMagíster en Ciencias - MicrobiologíaBiopolímeros y Fermentacionesxix, 119 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - BioquímicaPolihidroxialcanoatosBiopolímerosPoliésteresVectores GenéticosPseudomonas fluorescensBurkholderiaPolyhydroxyalkanoatesBiopolymersPolyestersGenetic VectorsPolyhydroxyalkanoatesPHAscopolymerBurkholderiaPseudomonasshort and medium chainPolihidroxialcanoatosPHAscopolímeroBurkholderiaPseudomonasCadena corta y mediaEvaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)Evaluation of a transformation vector in p. fluorescens ibun S1602 for production of a short chain (PHAscl) and medium chain (PHAmcl) polyhydroxyalcanoates (PHAs) copolymerTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAlvarez-Santullano, N., Villegas, P., Mardones, M. 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Biotechnology and Bioprocess Engineering, 18(3), 472–478. https://doi.org/10.1007/s12257-012-0607-zEstudiantesInvestigadoresMaestrosLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85280/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINALTesis de Maestría en Ciencias - Microbiología.pdfTesis de Maestría en Ciencias - Microbiología.pdfapplication/pdf3447788https://repositorio.unal.edu.co/bitstream/unal/85280/2/Tesis%20de%20Maestr%c3%ada%20en%20Ciencias%20-%20Microbiolog%c3%ada.pdfacfcc8d53bef9e92216d0427638617f2MD52THUMBNAILTesis de Maestría en Ciencias - Microbiología.pdf.jpgTesis de Maestría en Ciencias - Microbiología.pdf.jpgGenerated Thumbnailimage/jpeg5010https://repositorio.unal.edu.co/bitstream/unal/85280/3/Tesis%20de%20Maestr%c3%ada%20en%20Ciencias%20-%20Microbiolog%c3%ada.pdf.jpgf589570ffb8c0195394483920cb95231MD53unal/85280oai:repositorio.unal.edu.co:unal/852802024-01-15 23:04:09.876Repositorio Institucional 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Evaluación de un vector de transformación en p. fluorescens ibun S1602 para la producción de un copolímero de polihidroxialcanoatos (PHAs) de cadena corta (PHAscl) y cadena media (PHAmcl)

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