Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca

ilustraciones, diagramas, mapas

Autores:: González Bello, Diego Alejandro

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca
dc.title.translated.eng.fl_str_mv	Analysis of the fungal microbiome present in cocoa soils with different cadmium concentrations
title	Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca
spellingShingle	Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca 630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Microbiomas Acondicionadores del suelo Microbiomes Soil conditioners Comunidad-fúngica Theobroma-cacao Ecología-microbiana Metataxonomía Metales-pesados Fungal-community Theobroma-cacao Microbial-ecology Metataxonomy Heavy-metals Theobroma-cacao
title_short	Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca
title_full	Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca
title_fullStr	Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca
title_full_unstemmed	Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca
title_sort	Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca
dc.creator.fl_str_mv	González Bello, Diego Alejandro
dc.contributor.advisor.none.fl_str_mv	Torres Rojas, Esperanza Caro Quintero, Alejandro
dc.contributor.author.none.fl_str_mv	González Bello, Diego Alejandro
dc.contributor.researchgroup.spa.fl_str_mv	Agrobiodiversidad y Biotecnología
dc.contributor.orcid.spa.fl_str_mv	González Bello, Diego [0000000268915907]
dc.subject.ddc.spa.fl_str_mv	630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales
topic	630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación 630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materiales Microbiomas Acondicionadores del suelo Microbiomes Soil conditioners Comunidad-fúngica Theobroma-cacao Ecología-microbiana Metataxonomía Metales-pesados Fungal-community Theobroma-cacao Microbial-ecology Metataxonomy Heavy-metals Theobroma-cacao
dc.subject.agrovoc.spa.fl_str_mv	Microbiomas Acondicionadores del suelo
dc.subject.agrovoc.eng.fl_str_mv	Microbiomes Soil conditioners
dc.subject.proposal.spa.fl_str_mv	Comunidad-fúngica Theobroma-cacao Ecología-microbiana Metataxonomía Metales-pesados
dc.subject.proposal.eng.fl_str_mv	Fungal-community Theobroma-cacao Microbial-ecology Metataxonomy Heavy-metals Theobroma-cacao
description	ilustraciones, diagramas, mapas
publishDate	2023
dc.date.accessioned.none.fl_str_mv	2023-07-31T20:04:00Z
dc.date.available.none.fl_str_mv	2023-07-31T20:04:00Z
dc.date.issued.none.fl_str_mv	2023-07-24
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/84379
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/84379 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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S., Tian, X.-G., Xiong, Y.-R., Karunarathna, S. C., Cai, L., Luo, Z.-L., Wang, Y., Manawasinghe, I. S., … Hyde, K. D. (2022). The numbers of fungi: Contributions from traditional taxonomic studies and challenges of metabarcoding. Fungal Diversity, 114(1), 327-386. https://doi.org/10.1007/s13225-022-00502-3 Lehmann, A., Zheng, W., Ryo, M., Soutschek, K., Roy, J., Rongstock, R., Maaß, S., & Rillig, M. C. (2020). Fungal traits important for soil aggregation. Frontiers in Microbiology, 10, 2904. https://doi.org/10.3389/fmicb.2019.02904 Li, F., Jin, Z., Wang, Z., Liao, Y., Yu, L., & Li, X. (2022). Host plant selection imprints structure and assembly of fungal community along the soil-root continuum. MSystems, 7(4), e00361-22. https://doi.org/10.1128/msystems.00361-22 Li, B., Xu, R., Sun, X., Han, F., Xiao, E., Chen, L., Qiu, L., & Sun, W. (2021). 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(2010). Small-scale diversity and succession of fungi in the detritusphere of rye residues. Microbial Ecology, 59(1), 130-140. https://doi.org/10.1007/s00248-009-9541-9 Proulx, S., Promislow, D., & Phillips, P. (2005). Network thinking in ecology and evolution. Trends in Ecology & Evolution, 20(6), 345-353. https://doi.org/10.1016/j.tree.2005.04.004 Li, H., Dong, X., da Silva, E. B., de Oliveira, L. M., Chen, Y., & Ma, L. Q. (2017). Mechanisms of metal sorption by biochars: Biochar characteristics and modifications. Chemosphere, 178, 466-478. https://doi.org/10.1016/j.chemosphere.2017.03.072 Liang, G., Gong, W., Li, B., Zuo, J., Pan, L., & Liu, X. (2019). Analysis of heavy metals in foodstuffs and an assessment of the health risks to the general public via consumption in beijing, china. International Journal of Environmental Research and Public Health, 16(6), 909. https://doi.org/10.3390/ijerph16060909 Liao, M., Luo, Y., Zhao, X., & Huang, C. (2005). Toxicity of cadmium to soil microbial biomass and its activity: Effect of incubation time on Cd ecological dose in a paddy soil. Journal of Zhejiang University-SCIENCE B, 6(5), 324-330. https://doi.org/10.1631/jzus.2005.B0324 Lin, Y.-F., & Aarts, M. G. M. (2012). The molecular mechanism of zinc and cadmium stress response in plants. Cellular and Molecular Life Sciences, 69(19), 3187-3206. https://doi.org/10.1007/s00018-012-1089-z Luo, J.-S., & Zhang, Z. (2021). Mechanisms of cadmium phytoremediation and detoxification in plants. The Crop Journal, 9(3), 521-529. https://doi.org/10.1016/j.cj.2021.02.001 Luo, J., Xiao, X., & Luo, sheng-lian. (2010). Biosorption of cadmium(Ii) from aqueous solutions by industrial fungus Rhizopus cohnii. Transactions of Nonferrous Metals Society of China, 20(6), 1104-1111. https://doi.org/10.1016/S1003-6326(09)60264-8 Lux, A., Martinka, M., Vaculik, M., & White, P. J. (2011). Root responses to cadmium in the rhizosphere: A review. 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R., Ghosh, S., Cason, E. D., Nyaga, M. M., & Gryzenhout, M. (2022). Plant and soil core mycobiomes in a two-year sorghum–legume intercropping system of underutilized crops in south africa. Microorganisms, 10(10), 2079. https://doi.org/10.3390/microorganisms10102079 Pereira de Araújo, R., Furtado de Almeida, A.-A., Silva Pereira, L., Mangabeira, P. A. O., Olimpio Souza, J., Pirovani, C. P., Ahnert, D., & Baligar, V. C. (2017). Photosynthetic, antioxidative, molecular and ultrastructural responses of young cacao plants to Cd toxicity in the soil. Ecotoxicology and Environmental Safety, 144, 148-157. https://doi.org/10.1016/j.ecoenv.2017.06.006 Peršoh, D. (2015). Plant-associated fungal communities in the light of meta’omics. Fungal Diversity, 75(1), 1-25. https://doi.org/10.1007/s13225-015-0334-9 Praveen, R., & Nagalakshmi, R. (2022). Review on bioremediation and phytoremediation techniques of heavy metals in contaminated soil from dump site. 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dc.format.extent.spa.fl_str_mv	204 páginas
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dc.coverage.country.none.fl_str_mv	Colombia
dc.coverage.region.none.fl_str_mv	Yacopí Cundinamarca
dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias Agrarias - Maestría en Ciencias Agrarias
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias Agrarias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Torres Rojas, Esperanzadab829cf50bdc7b183de0183f2ebe854Caro Quintero, Alejandro62821879e223aa58e3168a169ceee82cGonzález Bello, Diego Alejandro41f238563130a3b363a98d154f9fe037Agrobiodiversidad y BiotecnologíaGonzález Bello, Diego [0000000268915907]2023-07-31T20:04:00Z2023-07-31T20:04:00Z2023-07-24https://repositorio.unal.edu.co/handle/unal/84379Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, mapasEl cadmio (Cd) es un metal pesado tóxico que altera el crecimiento y desarrollo de todos los organismos presentes en los sistemas agrícolas. Las plantas de cacao acumulan Cd y se han registrado altas concentraciones en los granos de cacao, lo que afecta la seguridad alimentaria y su comercialización. La comunidad microbiana del suelo cumple un papel importante en el ciclaje de nutrientes y en la biorremediación de suelos contaminados con metales pesados. Dentro de esta comunidad se destacan los hongos, que establecen redes miceliales que colonizan el suelo y exhiben mecanismos de tolerancia que les permiten sobrevivir altas concentraciones del metal. Sin embargo, pocos estudios han evaluado el efecto de las propiedades fisicoquímicas del suelo y del Cd sobre la comunidad fúngica presente en suelos cacaoteros. El objetivo de este estudio fue caracterizar la diversidad estructural de los microbiomas fúngicos presentes en suelos cacaoteros con diferentes propiedades físicas, químicas y concentraciones de Cd del municipio de Yacopí-Cundinamarca. Para ello, se seleccionaron tres fincas cacaoteras con diferentes concentraciones de Cd (F1>5,0 mg kg-1; F2 y F3<2 mg kg-1) y dos lotes uno con cultivo de cacao (SCC) y otro sin cultivo de cacao (SWC) en cada finca. Se colectaron muestras de suelo rizosférico (Rz) y no Rz a dos profundidades, D1 (0-30 cm) y D2 (31-100 cm); y muestras de hojarasca (HJ), hojas (HF) y frutos en los lotes SCC. Para los lotes SWC se colectaron únicamente suelo a las profundidades D1 y D2. (i) Se determinaron las propiedades fisicoquímicas del suelo y su relación con el Cd en suelos, hojarasca, hoja y grano de cacao en las tres fincas. Se encontró que la distribución de Cd fue heterogénea y mayor en F1>F3>F2, y en los suelos de F1 los contenidos de Cd fueron mayores SCC>SWC y en suelos Rz>D1=D2, alcanzado un valor promedio de 10,24 mg kg-1. Las concentraciones de Cd presentes en los suelos de las tres fincas fueron superiores a los niveles establecidos por las entidades regulatorias internacionales para suelos agrícolas no contaminados (1,0 mg kg-1) y el contenido de Cd en granos fue superior a las concentraciones permitidas por la comunidad europea para grano seco (0,6 mg kg-1). La concentración de Cd en los tejidos vegetales analizados fue mayor en HJ>HF>G. También el contenido de Cd en la HJ fue mayor a lo encontrado en el suelo a profundidad de D1, lo que podría contribuir a la recirculación del Cd en sistema suelo-planta. Posteriormente, (ii) se caracterizó la riqueza y abundancia relativa de la comunidad fúngica presentes en los suelos analizados. Los resultados muestran que la riqueza y la abundancia relativa encontrada en SCC fue mayor que la presente en SWC, indicando que el cultivo de cacao crea ambientes diferentes que favorecen la diversidad. Los ASVs (taxones) encontrados en los suelos de la finca F1 mostraron menor similitud con respecto a los presentes en los suelos de las fincas F2 y F3, mayor número de ASVs únicos en suelos con cultivo (SCC) y en suelos Rz; lo que indica que la estructura de la comunidad fúngica se ve alterada por las propiedades fisicoquímicas, el contenido de Cd en el suelo, el cultivo de cacao y la rizosfera. Los phylum Ascomycota (68%) y Basidiomycota (25%) fueron los más abundantes, donde los órdenes Hypocreales, Pleosporales y Capnodiales fueron los más representativos. Adicionalmente, los análisis de abundancia diferencial destacan los géneros Hypoxylon, Microdochium, Xylaria y la familia Nectricaceae, los cuales han sido reportados como tolerantes a metales pesados. También se encontraron géneros raros y pocos conocidos como Plectosphaerella, Tetracladium, Microdihum y Cyberlindnera que podrían tener capacidad de tolerar altas concentraciones de Cd. Finalmente, (iii) se evaluó el efecto de las características fisicoquímicas y concentraciones de Cd presente en suelos cacaoteros sobre la diversidad estructural de las comunidades fúngicas. Se encontró que las propiedades fisicoquímicas de los suelos presentaron bajas correlaciones con los índices de diversidad alfa, indicando que la estructura de las comunidades fúngicas está determinada por múltiples factores. La textura, el pH, CO, N, Na y Cdt mostraron tener mayor efecto en la diversidad estructural. Las redes de co-ocurrencia mostraron géneros fúngicos únicos y compartidos por finca. A su vez, se encontró que la finca F1 se presentó una estructura más homogénea debido posiblemente a los altos contenido de Cd y propiedades fisicoquímicas encontradas, en contraste, la finca F2, la estructura cambia de acuerdo a la profundidad del suelo. Esta investigación aporta por primera vez al conocimiento de la diversidad de la comunidad fúngica presente en SCC y SWC enriquecido con concentraciones de Cd natural. (Texto tomado de la fuente)Cadmium (Cd) is a toxic heavy metal that alters the growth and development of all organisms present in agricultural systems. Cacao plants accumulate Cd and high concentrations have been recorded in cacao beans, affecting food safety and marketing. The soil microbial community plays an important role in nutrient cycling and in the bioremediation of soils contaminated with heavy metals. Within this community, fungi stand out, establishing mycelial networks that colonize the soil and exhibit tolerance mechanisms that allow them to survive high concentrations of this metal. However, few studies have evaluated the effect of soil physicochemical properties and Cd on the fungal community present in cacao soils. The objective of this study was to characterize the structural diversity of fungal microbiomes present in cocoa soils with different physical and chemical properties and Cd concentrations in the municipality of Yacopí-Cundinamarca. For this purpose, three cocoa farms with different Cd concentrations (F1>5,0 mg kg-1 ; F2 and F3<2,0 mg kg-1 ) and two lots, one with cacao crop (SCC) and one without cacao crop (SWC) were selected in each farm. Rhizospheric (Rz) and non-Rz soil samples were collected at two depths, D1 (0-30 cm) and D2 (31-100 cm); and leaf litter (HJ), leaf (HF) and pods samples were collected in the SCC plots. For the SWC plots, only soil was collected at depths D1 and D2. (i) The physicochemical properties of the soil and their relationship with Cd in soils, leaf litter, leaves and cacao beans were determined on the three farms. It was found that the distribution of Cd was heterogeneous and higher in F1>F3>F2, and in F1 soils the Cd contents were higher SCC>SWC and in Rz>D1=D2 soils, reaching an average value of 10.24 mg kg-1 . The Cd concentrations present in the soils of the three farms were higher than the levels established by international regulatory for non-contaminated agricultural soils (1.0 mg kg-1) and the Cd content in grains was higher than the concentrations allowed by the European community for dry beans (0.6 mg kg-1). The Cd concentration in the plant tissues analyzed was higher in HJ>HF>G. Also, the Cd content in HJ was higher than that found in the soil at the depth of D1, which could contribute to the recirculation of Cd in the soil-plant system. Subsequently, (ii) the richness and relative abundance of the fungal community present in the analyzed soils were characterized. The results show that the richness and relative abundance found in SCC was higher than that present in SWC, indicating that cacao cultivation creates different environments that favor diversity. The ASVs (taxa) found in F1 farm soils showed lower similarity with respect to those present in F2 and F3 farm soils, higher number of unique ASVs in SCC and in Rz soils; indicating that the fungal community structure is altered by physicochemical properties, soil Cd content, cacao crop and rhizosphere. The phylum Ascomycota (68%) and Basidiomycota (25%) were the most abundant, where the orders Hypocreales, Pleosporales and Capnodiales were the most representative. In addition, differential abundance analyses highlighted the genera Hypoxylon, Microdochium, Xylaria and the family Nectricaceae, which have been reported to be tolerant to heavy metals. Rare and little known genera such as Plectosphaerella, Tetracladium, Microdihum and Cyberlindnera were also found that could be able to tolerate high Cd concentrations. Finally, (iii) the effect of physicochemical characteristics and Cd concentrations present in cacao soils on the structural diversity of fungal communities was evaluated. It was found that the physicochemical properties of the soils presented low correlations with the alpha diversity indices, indicating that the structure of fungal communities is determined by multiple factors. Texture, pH, organic carbon, N, Na and Cdt were shown to have the greatest effect on structural diversity. Co-occurrence networks showed unique and shared fungal genera per farm. At the same time, it was found that the F1 farm presented a more homogeneous structure, possibly due to the high Cd content and physicochemical properties found, in contrast, the F2 farm, the structure changes according to soil depth. This research contributes for the first time to the knowledge of the diversity of the fungal community present in SCC and SWC enriched with natural Cd concentrations.MaestríaMagíster en Ciencias Agrarias204 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias Agrarias - Maestría en Ciencias AgrariasFacultad de Ciencias AgrariasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación630 - Agricultura y tecnologías relacionadas::631 - Técnicas específicas, aparatos, equipos, materialesMicrobiomasAcondicionadores del sueloMicrobiomesSoil conditionersComunidad-fúngicaTheobroma-cacaoEcología-microbianaMetataxonomíaMetales-pesadosFungal-communityTheobroma-cacaoMicrobial-ecologyMetataxonomyHeavy-metalsTheobroma-cacaoAnálisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - CundinamarcaAnalysis of the fungal microbiome present in cocoa soils with different cadmium concentrationsTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMColombiaYacopíCundinamarcaAbatenh, E., Gizaw, B., Tsegaye, Z., Wassie, M., Abatenh, E., Gizaw, B., Tsegaye, Z., & Wassie, M. 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Food and Agriculture Organization of the United Nations.Caracterización estructural y predicción funcional de la comunidad microbiana presente en suelos con diferentes niveles de cadmio en fincas cacaoteras del Municipio de Yacopí-CundinamarcaUniversidad Nacional de ColombiaEstudiantesInvestigadoresMaestrosPúblico generalORIGINAL1032466943.2023.pdf1032466943.2023.pdfTesis de Maestría en Ciencias Agrariasapplication/pdf5431005https://repositorio.unal.edu.co/bitstream/unal/84379/2/1032466943.2023.pdf1caac842ce17f4034dba4b059b67e25dMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84379/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53THUMBNAIL1032466943.2023.pdf.jpg1032466943.2023.pdf.jpgGenerated Thumbnailimage/jpeg5090https://repositorio.unal.edu.co/bitstream/unal/84379/4/1032466943.2023.pdf.jpg715d375a27d1d89de9748a06f3689dc0MD54unal/84379oai:repositorio.unal.edu.co:unal/843792023-08-12 23:04:08.018Repositorio Institucional 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Análisis del microbioma fúngico presente en diferentes tipos de suelos cacaoteros con cadmio en el municipio de Yacopí - Cundinamarca

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