Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia
ilustraciones, diagramas, mapa
- Autores:
-
Polanco Rodríguez, María Camila
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/85587
- Palabra clave:
- 570 - Biología::577 - Ecología
590 - Animales::597 - Vertebrados de sangre fría
Flora intestinal
Intestines-Microbiology
Flora microbiana
Cocodrilos
Microbial flora
Crocodylidae
Microbiota
Cocodrilos
Heces
Reptiles
Crocodile
Feces
Gut
Crocodylus intermedius
Microbiota
- Rights
- openAccess
- License
- Reconocimiento 4.0 Internacional
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oai:repositorio.unal.edu.co:unal/85587 |
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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|
| dc.title.spa.fl_str_mv |
Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia |
| dc.title.translated.eng.fl_str_mv |
Characterization of the gut bacterial microbiome of the critically endangered Orinoco Crocodile (Crocodylus intermedius), present in the largest ex situ population at the Roberto Franco Tropical Biology Station (EBTRF) in Villavicencio, Colombia |
| title |
Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia |
| spellingShingle |
Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia 570 - Biología::577 - Ecología 590 - Animales::597 - Vertebrados de sangre fría Flora intestinal Intestines-Microbiology Flora microbiana Cocodrilos Microbial flora Crocodylidae Microbiota Cocodrilos Heces Reptiles Crocodile Feces Gut Crocodylus intermedius Microbiota |
| title_short |
Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia |
| title_full |
Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia |
| title_fullStr |
Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia |
| title_full_unstemmed |
Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia |
| title_sort |
Caracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, Colombia |
| dc.creator.fl_str_mv |
Polanco Rodríguez, María Camila |
| dc.contributor.advisor.none.fl_str_mv |
Vargas Ramírez, Mario |
| dc.contributor.author.none.fl_str_mv |
Polanco Rodríguez, María Camila |
| dc.contributor.subjectmatterexpert.none.fl_str_mv |
Gómez Nieto, Diego |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::577 - Ecología 590 - Animales::597 - Vertebrados de sangre fría |
| topic |
570 - Biología::577 - Ecología 590 - Animales::597 - Vertebrados de sangre fría Flora intestinal Intestines-Microbiology Flora microbiana Cocodrilos Microbial flora Crocodylidae Microbiota Cocodrilos Heces Reptiles Crocodile Feces Gut Crocodylus intermedius Microbiota |
| dc.subject.lcc.spa.fl_str_mv |
Flora intestinal |
| dc.subject.lcc.eng.fl_str_mv |
Intestines-Microbiology |
| dc.subject.lemb.spa.fl_str_mv |
Flora microbiana Cocodrilos |
| dc.subject.lemb.eng.fl_str_mv |
Microbial flora Crocodylidae |
| dc.subject.proposal.spa.fl_str_mv |
Microbiota Cocodrilos Heces Reptiles |
| dc.subject.proposal.eng.fl_str_mv |
Crocodile Feces Gut Crocodylus intermedius |
| dc.subject.wikidata.spa.fl_str_mv |
Microbiota |
| description |
ilustraciones, diagramas, mapa |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-02-01T16:09:27Z |
| dc.date.available.none.fl_str_mv |
2024-02-01T16:09:27Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/85587 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/85587 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Vargas Ramírez, Marioed5b1d0e018fc833079772b4f20c24d0Polanco Rodríguez, María Camilafade8f0e64e63d30e60dbe14b5960414Gómez Nieto, Diego2024-02-01T16:09:27Z2024-02-01T16:09:27Z2023https://repositorio.unal.edu.co/handle/unal/85587Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, mapaEl Cocodrilo del Orinoco (Crocodylus intermedius) es un reptil endémico de zonas bajas de la cuenca del río Orinoco. Actualmente la especie enfrenta serias amenazas para su supervivencia, por lo cual es necesario un plan de conservación integral que incluya programas modernos como el mantenimiento de un microbioma saludable, lo cual puede mejorar su mantenimiento, reproducción y liberación. Este estudio tuvo como objetivo caracterizar la microbiota bacteriana de las heces de C. intermedius, mantenidos en la mayor población ex-situ colombiana (EBTRF, Villavicencio, Meta) y comparar las poblaciones bacterianas entre cocodrilos juveniles y adultos. Se realizó la secuenciación del gen rRNA 16S de 24 muestras de materia fecal (Juveniles n=15; Adultos n=9), utilizando el equipo Illumina MiSeq (2×250 bp). Los resultados indican que la microbiota fecal de está predominada por Firmicutes, seguido por Bacteroidetes y Proteobacterias. Los índices de medición de Alfa diversidad y la estructura bacteriana no tuvieron una diferencia significativa entre los Juveniles y Adultos, sin embargo, el membership de las poblaciones bacterianas difirió significativamente entre los grupos. La composición de la microbiota fecal de ambos estuvo fuertemente predominada por Firmicutes y Bacteroidetes, distribución que se ha relacionado con enfermedades como la obesidad y desórdenes endocrinos. A pesar de que investigaciones previas han reportado bacterias patógenas en la microbiota fecal, los reptiles poseen un fuerte sistema inmunológico que podría conferir una mayor resistencia a infecciones, cáncer y senescencia. Esta investigación presenta una herramienta para la conservación del Cocodrilo del Orinoco y amplía el conocimiento de la microbiota en reptiles. (Texto tomado de la fuente)The Orinoco Crocodile (Crocodylus intermedius) is an endemic reptile found in lowland areas of the Orinoco River basin. Currently, the species faces serious threats to its survival, demanding an all-encompassing conservation program that includes modern programs such as maintaining a healthy microbiome, which can improve its maintenance, reproduction, and release. This study aimed to characterize the bacterial microbiota of feces from C. intermedius kept in the largest ex-situ Colombian population (EBTRF, Villavicencio, Meta) and compare the bacterial populations between juvenile and adult crocodiles. The 16S rRNA gene sequencing was performed on 24 fecal samples (Juveniles n=15; Adults n=9), using the Illumina MiSeq platform (2×250 bp). The results indicate that the fecal microbiota is predominantly composed of Firmicutes, followed by Bacteroidetes and Proteobacteria in both groups. Alpha diversity and bacterial structure measurements did not show significant differences between juveniles and adults; however, the membership of bacterial populations differed significantly between the groups. The majority presence of Firmicutes and Bacteroidetes have been associated with diseases such as obesity and endocrine disorders in reptiles. Despite previous reports of pathogenic bacteria in the fecal microbiota, reptiles possess a robust immune system that may confer greater resistance to infections, cancer, and senescence. This research provides a tool for the conservation of the Orinoco Crocodile and expands our understanding of reptilian fecal microbiota.MaestríaMagíster en Ciencias - Microbiologíaxv, 62 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::577 - Ecología590 - Animales::597 - Vertebrados de sangre fríaFlora intestinalIntestines-MicrobiologyFlora microbianaCocodrilosMicrobial floraCrocodylidaeMicrobiotaCocodrilosHecesReptilesCrocodileFecesGutCrocodylus intermediusMicrobiotaCaracterización del microbioma bacteriano intestinal del críticamente amenazado Cocodrilo del Orinoco (Crocodylus intermedius) presente en la mayor población ex situ en la Estación de Biología Tropical Roberto Franco (EBTRF) en Villavicencio, ColombiaCharacterization of the gut bacterial microbiome of the critically endangered Orinoco Crocodile (Crocodylus intermedius), present in the largest ex situ population at the Roberto Franco Tropical Biology Station (EBTRF) in Villavicencio, ColombiaTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMColombiaArumugam, M., Raes, J., Pelletier, E., Le Paslier, D., Yamada, T., Mende, D. 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Microbial Pathogenesis, 180, 106148. https://doi.org/10.1016/j.micpath.2023.106148EstudiantesInvestigadoresMaestrosMedios de comunicaciónPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/85587/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1144075975.2023.pdf1144075975.2023.pdfTesis de Maestría en Ciencias - Microbiologíaapplication/pdf1391674https://repositorio.unal.edu.co/bitstream/unal/85587/2/1144075975.2023.pdf5958d3e53f4fe3f28414155243f2de04MD52THUMBNAIL1144075975.2023.pdf.jpg1144075975.2023.pdf.jpgGenerated Thumbnailimage/jpeg4990https://repositorio.unal.edu.co/bitstream/unal/85587/3/1144075975.2023.pdf.jpg1c87c66334cc4b5c0f8a674f6416604eMD53unal/85587oai:repositorio.unal.edu.co:unal/855872024-08-22 23:10:30.209Repositorio Institucional Universidad Nacional de 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