Detección molecular y limpieza de virus en material de siembra de Solanum tuberosum y S. phureja
ilustraciones, tablas
- Autores:
-
García Torres, Andrea Stefania
- Tipo de recurso:
- Fecha de publicación:
- 2022
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/82244
- Palabra clave:
- 570 - Biología::575 - Partes específicas de y sistemas fisiológicos en plantas
630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales
630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación
Virología agrícola
Papa - Enfermedades y plagas
Papa - Cultivo
Cultivo in vitro
Certificación de semilla
Secuenciación de alto rendimiento
Virología vegetal
Papa (Solanum tuberosum y S. phureja)
qPCR
High-throughput sequencing
Plant virology
Seed certification
Solanaceae
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Detección molecular y limpieza de virus en material de siembra de Solanum tuberosum y S. phureja |
| dc.title.translated.eng.fl_str_mv |
Molecular detection and characterization of viruses and development of in vitro virus removal methods in Solanum tuberosum and S. phureja |
| title |
Detección molecular y limpieza de virus en material de siembra de Solanum tuberosum y S. phureja |
| spellingShingle |
Detección molecular y limpieza de virus en material de siembra de Solanum tuberosum y S. phureja 570 - Biología::575 - Partes específicas de y sistemas fisiológicos en plantas 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales 630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación Virología agrícola Papa - Enfermedades y plagas Papa - Cultivo Cultivo in vitro Certificación de semilla Secuenciación de alto rendimiento Virología vegetal Papa (Solanum tuberosum y S. phureja) qPCR High-throughput sequencing Plant virology Seed certification Solanaceae |
| title_short |
Detección molecular y limpieza de virus en material de siembra de Solanum tuberosum y S. phureja |
| title_full |
Detección molecular y limpieza de virus en material de siembra de Solanum tuberosum y S. phureja |
| title_fullStr |
Detección molecular y limpieza de virus en material de siembra de Solanum tuberosum y S. phureja |
| title_full_unstemmed |
Detección molecular y limpieza de virus en material de siembra de Solanum tuberosum y S. phureja |
| title_sort |
Detección molecular y limpieza de virus en material de siembra de Solanum tuberosum y S. phureja |
| dc.creator.fl_str_mv |
García Torres, Andrea Stefania |
| dc.contributor.advisor.none.fl_str_mv |
Hoyos Sánchez, Rodrigo Alberto Gutiérrez Sánchez, Pablo Andrés |
| dc.contributor.author.none.fl_str_mv |
García Torres, Andrea Stefania |
| dc.contributor.educationalvalidator.none.fl_str_mv |
Marìn Montoya, Mauricio Alejandro Correa Londoño, Guillermo Antonio |
| dc.contributor.researchgroup.spa.fl_str_mv |
Biotecnología Microbiana Biotecnología Vegetal Unalmed Cib |
| dc.subject.ddc.spa.fl_str_mv |
570 - Biología::575 - Partes específicas de y sistemas fisiológicos en plantas 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales 630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación |
| topic |
570 - Biología::575 - Partes específicas de y sistemas fisiológicos en plantas 630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetales 630 - Agricultura y tecnologías relacionadas::633 - Cultivos de campo y de plantación Virología agrícola Papa - Enfermedades y plagas Papa - Cultivo Cultivo in vitro Certificación de semilla Secuenciación de alto rendimiento Virología vegetal Papa (Solanum tuberosum y S. phureja) qPCR High-throughput sequencing Plant virology Seed certification Solanaceae |
| dc.subject.lemb.none.fl_str_mv |
Virología agrícola Papa - Enfermedades y plagas Papa - Cultivo Cultivo in vitro |
| dc.subject.proposal.spa.fl_str_mv |
Certificación de semilla Secuenciación de alto rendimiento Virología vegetal Papa (Solanum tuberosum y S. phureja) |
| dc.subject.proposal.eng.fl_str_mv |
qPCR High-throughput sequencing Plant virology Seed certification |
| dc.subject.proposal.other.fl_str_mv |
Solanaceae |
| description |
ilustraciones, tablas |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-09-02T21:32:26Z |
| dc.date.available.none.fl_str_mv |
2022-09-02T21:32:26Z |
| dc.date.issued.none.fl_str_mv |
2022-08-20 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
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http://purl.org/redcol/resource_type/TM |
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acceptedVersion |
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https://repositorio.unal.edu.co/handle/unal/82244 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/82244 https://repositorio.unal.edu.co/ |
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Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
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The comparison of antibodies raised against PLRV with two different approaches - viral particles purification and recombinant production of CP. Journal of Plant Pathology & Microbiology, 8, 5. https://doi.org/10.4172/2157-7471.1000407 Avrahami-Moyal, L., Tam, Y., Brumin, M., Prakash, S., Leibman, D., Pearlsman, M., Bornstein, M., Sela, N., Zeidan, M., Dar, Z., Zig, U., Gal-On, A. y Gaba, V. (2017). Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR. Phytoparasitica, 45, 591-598. https://doi.org/10.1007/s12600-017-0612-z. Azcón-Bieto, J. y Talón, M. (2013). Fundamentos de fisiología vegetal. (2a ed.). Barcelona. Bahner, L. Lamb, J., Mayo, M. A. y Hay R. T. (1990). Expression of the genome of potato leafroll virus: readthrough of the coat protein termination codon in vivo. Journal of General Virology, 71, 2251-2256. Bains, P. S., Bennypaul, H. S., Lynch, D. R., Kawchuk, L. M. y Schaupmeyer, C. A. (2002). 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Elaboración de una bebida alcohólica destilada (Vodka) a partir de tres variedades de papa (Solanum tuberosum) utilizando dos tipos de enzima. Universidad Técnica del Norte. http://repositorio.utn.edu.ec/handle/123456789/327 Bhat, A. I. y Rao, G.P. (2020). Characterization of Plant Viruses. Springer Protocols Handbooks. https://link.springer.com/book/10.1007/978-1-0716-0334-5 Birch, P. R. J., Bryan, G., Fenton, B., Gilroy, E. M., Hein, I., Jones, J. T., Prashar, A., Taylor, M., Torrance, L y Toth, I. (2012). Crops that feed the world 8: Potato: Are the trends of increased global production sustainable?. Food Security. 4, 477–508. https://doi.org/10.1007/s12571-012-0220-1. Blanc, S. (2008). Vector transmission of plant viruses. Mahy, B. W.y Van Regenmortel, M. H. (Ed). Desk encyclopedia of plant and fungal Virology. (pp. 35-48). New York: Academic Press. Bokelmann, G. S. y Roest, S. (1983). Plant Regeneration from Protoplasts of Potato (Solarium tuberosum cv. Bintje). Zeitschrift Für Pflanzenphysiologie, 109, 259-265. https://doi.org/10.1016/s0044-328x(83)80228-1. Boonham, N., Kreuze, J., Winter, S., van der Vlugt, R., Bergervoet, J., Tomlinson, J. y Mumford, R. (2014). Methods in virus diagnostics: From ELISA to next generation sequencing. Virus Research, 186, 20-31. https://doi.org/10.1016/j.virusres.2013.12.007 Boratyn, G., Thierry-Mieg, J., Thierry-Mieg, D., Busby, B. y Madden, T. (2019). Magic-BLAST, an accurate RNA-seq aligner for long and short reads. BMC Bioinformatics, 20. https://doi.org/10.1186/s12859-019-2996-x. Braun, C. J. y Hemenway, C. L. (1992). Expression of amino-terminal portions or full-length vira1 replicase genes in transgenic plants confers resistance to Potato Virus X infection. Plant Cell, 4, 735–44. https://doi.org/10.1105/tpc.4.6.735 Brown, J., Pirrung, M. y Mccue, L. (2017). FQC Dashboard: Integrates FastQC results into a web-based, interactive, and extensible FASTQ quality control tool. Bioinformatics, 33, 3137-3139. https://doi.org/10.1093/bioinformatics/btx373 Burlingame, B., Mouillé, B. y Charrondière, R. (2009). Nutrients, bioactive non-nutrients and anti-nutrients in potatoes. Journal of Food Composition and Analysis, 22, 494–502. https://doi.org/10.1016/j.jfca.2009.09.001 Bushmanova, E., Antipov, D., Lapidus, A. y D Prjibelski, A. (2019). rnaSPAdes: a de novo transcriptome assembler and its application to RNA-Seq data, GigaScience, 8(9), 1-13. https://doi.org/10.1093/gigascience/giz100 Camacho, C., Coulouris, G., Avagyan, Ma, N., Papadopoulos, J., Bealer, K. y Madden, T. L. (2009). BLAST+: architecture and applications. BMC Bioinformatics, 10(421). https://doi.org/10.1186/1471-2105-10-421 Campos, H. y Ortiz, O. (2020). The potato crop. Its agricultural, nutritional and social Contribution to Humankind. International Potato Center. Springer. https://doi.org/10.1007/978-94-011-2340-2 Carbajal, N. (2018). 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Bacterial Diseases of Potato. Campos, H. y Ortiz, O. (Ed). The potato crop. Its agricultural, nutritional and social Contribution to Humankind. (pp. 351-388). International Potato Center. Springer. https://doi.org/10.1007/978-94-011-2340-2 Chaves, S. C., Rodríguez, M. C., Mideros, M. F., Lucca, F., Ñústez, C. E. y Restrepo, S. (2019). Determining whether geographic origin and potato genotypes shape the population structure of Phytophthora infestans in the central region of Colombia. Phytopathology,109(1), 145–154. https://doi.org/10.1094/PHYTO-05-18-0157-R CIP. (2015a). Cómo crecen las papas - International Potato Center. Recuperado el 15 de abril de 2020 de https://cipotato.org/es/lapapa/como-crecen-las-papas/ CIP. (2015b). Dato y cifras de la papa - International Potato Center. Recuperado el 15 de abril de 2020 de https://cipotato.org/es/potato/potato-facts-and-figures/ Cobos, R. M., Becerra–Rozo, W. M. y Castellanos, G. L. (2019). Richness and abundance of the land slugs in four crops of Pamplona, Norte de Santander, Colombia. Bistua: Revista de la Facultad de Ciencias Básicas, 17(2), 229-233. https://doi.org/10.24054/01204211.v2.n2.2019.3538. Costa, T. M., Inoue-Nagata, A. K., Vidal, A. H., Ribeiro, S. G. y Nagata, T. (2020). The recombinant isolate of cucurbit aphid-borne yellows virus form Brazil is a polerovirus transmitted by whiteflies. Plant Pathology, 69(6), 1042-1050. https://doi.org/10.1111/ppa.13186 Cox, B. A. y Jones, R. A. C. (2010). Genetic variability in the coat protein gene of Potato virus X and the current relationship between phylogenetic placement and resistance groupings, Archives of Virology, 155, 1349-1356. https://doi.org/10.1007/s00705-010-0711-3 Crosslin, J. M., Hamlin, L. L., Buchman, J. L. y Munyaneza, J. E. (2011). Transmission of Potato Purple Top Phytoplasma to Potato Tubers and Daughter Plants. American Journal of Potato Research, 88, 339-345. https://doi.org/10.1007/s12230-011-9199-y Cubero, J. (2002). Introducción a la mejora genética vegetal. MundiPrensa. Dalca, A. y Brudno, M. (2010). Genome variation discovery with high-throughput sequencing data. Briefings in bioinformatics, 11(1), 3-14. https://doi.org/10.1093/bib/bbp058 Danci, O., Erdei, L., Vidacs, L., Danci, M., Baciu, A., David, I. y Berbentea, F. (2009) Influence of ribavirin on potato plants regeneration and virus eradication. Journal of Horticulture, Forestry and Biotechnology, 13, 421-425. Duarte, Y., Pino, O., Infante, D., Sánchez, Y., Travieso, C. y Martínez, B. (2013). Efecto in vitro de aceites esenciales sobre Alternaria solani Sorauer. Revista Protección Vegetal, 28(1), 54-59. Duarte-Delgado, D., Narváez-Cuenca, C., Restrepo-Sánchez, L., Kushalappa, A. y Mosquera-Vásquez, T. (2015). Development and validation of a liquid chromatographic method to quantify sucrose, glucose, and fructose in tubers of Solanum tuberosum Group Phureja. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 975, 18-23. https://doi.org/10.1016/j.jchromb.2014.10.039. Escallón, R., Ramírez, M. y Ñústez, C. (2005). Evaluación del potencial de rendimiento y de la resistencia a Phytophthora infestans (Mont. de Bary) en la colección de papas redondas amarillas de la especie Solanum phureja (Juz. et Buk.). Agronomía Colombiana, 23(1), 35-41. Elhiti, M., Stasolla, C. y Wang, A. (2013). Molecular regulation of plant somatic embryogenesis. In Vitro Cellular and Developmental Biology – Plant, 49, 631-642. https://doi.org/10.1007/s11627-013-9547-3. Ellis, D., Salas, A., Chavez, O., Gomez, R. y Anglin, N. (2020). Ex situ conservation of potato [Solanum section Petota (Solanaceae)] genetic resources in Genebanks. Campos, H. y Ortiz, O. (Ed). The potato crop. Its agricultural, nutritional and social Contribution to Humankind. (pp. 109-138). International Potato Center. Springer. https://doi.org/10.1007/978-94-011-2340-2 Estrada, R., Tovar, P. y Dodds, J. H. (1986). Induction of in vitro tubers in a broad range of potato genotypes. Plant Cell Tissue Organ Culture, 7, 3-10. https://doi.org/10.1007/BF00043915 Ewels, P., Magnusson, M., Lundin, S. y Käller, M. (2016). MultiQC: Summarize analysis results for multiple tools and samples in a single report. Bioinformatics, 32, 3047–3048. https://doi.org/10.1093/bioinformatics/btw354. FAO. (2008a). El mundo de la papa: Producción y consumo. Recuperado el 15 de abril de 2020 de http://www.fao.org/potato-2008/es/mundo/index.html FAO. (2008b). La papa: Cultivo. Recuperado el 17 de abril de 2020 de http://www.fao.org/potato-2008/es/lapapa/cultivo.html. Fedepapa. (2017). Normatividad del Sector de la Papa. Recuperado el 12 de abril de 2020 de https://fedepapa.com/wp-content/uploads/2017/01/Normatividad-del-Sector-de-la-Papa.pdf Fedepapa. (2018). Revista Papa: Una papa bien preparada te alegra, 43:48. Recuperado el 15 de abril de 2020 de https://drive.google.com/file/d/1lcX1XrthQdV6GmAHpX3ErrY3QoJyKBVf/view Fedepapa. (2019). Revista papa: Se trazó la ruta para consolidar la rentabilidad del sector agropecuario, 48, 46–52 Recuperado el 17 de abril del 2020 de https://fedepapa.com/wp-content/uploads/2017/01/REVISTA-48-COMPLETA.pdf FERA. (2017). Potato post-harvest virus testing sample submission form. Recuperado el 20 de noviembre del 2020 de https://www.fera.co.uk/media/wysiwyg/crop_health/Crop_Health_Post-Harvest_Virus_Testing_of_Potato_Tubers_Sample_Submission-2019.pdf Filiz, E. (2020). Emerging Plant Viruses. Ennaji, M. (Ed). Emerging and reemerging viral pathogens. (pp.1041-1062). New York: Academic Press. FNFP. (2006). Informe de gestión 2006, 136. Recuperado el 21 de abril de 2020 de https://fedepapa.com/wpcontent/uploads/2017/01/INFORME-DE-GESTIO%CC%81N-FNFP-ANUAL-2016.pdf FNFP y Fedepapa. (2019a). Informe trimestral de coyuntura económica del subsector papa II trimestre – 2019. Recuperado el 15 de abril de 2020 de https://fedepapa.com/wp-content/uploads/2017/01/Informe-deCoyuntura-2do-trimestre-2019.pdf FNFP y Fedepapa. (2019b). Informe de gestión. Vigencia 2019. Recuperado el 5 de junio de 2021 de https://fedepapa.com/wp-content/uploads/2020/05/INFORME-DE-GESTIO%CC%81N-VIGENCIA-2019.pdf FNFP y Fedepapa. (2020). Boletin regional No. 05. Recuperado el 24 de abril de 2022 de https://fedepapa.com/wp-content/uploads/2021/09/NACIONAL-2020.pdf Forbes, G. A., Charkowski, A., Andrade-Piedra, J., Parker, M. L. y Schulte-Geldermann, E. (2020). Potato seed systems. Campos, H. y Ortiz, O. (Ed). The potato crop. Its agricultural, nutritional and social Contribution to Humankind. (pp. 431-450). International Potato Center. 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Prevalencia de cinco virus de ARN en tubérculos-semilla de papa cultivados en Antioquia (Colombia). Biotecnología en el Sector Agropecuario y Agroindustrial 19(1): 66-78. https://dx.doi.org/10.18684 Ghislain, M., D. Andrade, F. Rodríguez, R.J. Hijmans, D.M. Spooner. 2006. Genetic analysis of the cultivated potato Solanum tuberosum L. Phureja Group using RAPDs and nuclear SSRs. Theoretical and Applied Genetics 113(8): 1515-1527. https://10.1007/s00122-006-0399-7 Giraldo, S., A. Sierra, M. Ospina, M. Higuita, Y. Gallo, P. Gutiérrez, M. Marín. 2022. Detección y caracterización molecular del potato virus B (PVB) en papa criolla (Solanum phureja) en Antioquia. Acta Biológica Colombiana 27(2): 258-268. https://doi.org/10.15446/abc.v27n2.89422 Herrera, A.O., L.E. Rodríguez. 2011. Tecnologías de Producción y Transformación de Papa Criolla. Universidad Nacional de Colombia. Bogotá. ISBN : 978-958-761-110-6 Kumar, R., P. Kaundal, R. Kumar, S. Siddappa, H. Kumari, K. Chandra, S. Sharma, M. Kumar. 2021. Rapid and sensitive detection of potato virus X by one-step reverse transcription-recombinase polymerase amplification method in potato leaves and dormant tubers. Molecular and Cellular Probes 58: 101743. https:// 10.1016/j.mcp.2021.101743 Mortimer-Jones, S.M., M.G. Jones, R.A. Jones, G. Thomson, G.I. Dwyer. 2009. A single tube, quantitative real-time RT-PCR assay that detects four potato viruses simultaneously. Journal of Virological Methods 161: 289–296. https://10.1016/j.jviromet.2009.06.027 NAK. 2015. Details virus and bacterial diagnostics in potatoes 2016–2017. [accessed 2020 Sept 10]. https://www.nak.nl/wpcontent/uploads/archief/2012/NAK%20Services/Virus%20and%20bacterial%20diagnostics%20in%20potatoes%202017-2018.pdf. Ñustez, C.E. 2011. Variedades Colombianas de Papa. Bogotá: Universidad Nacional de Colombia. 46 p. Seminario, J.F., R. Villanueva-Guevara, M.H. Valdez-Yopla. 2018. Rendimiento de cultivares de papa (Solanum tuberosum L.) amarillos precoces del grupo Phureja. Agronomía Mesoamericana 29(3): 639-653. https://10.15517/ma.v29i3.32623 Sierra A., Y. Gallo, M. Estrada, P. Gutiérrez, M. Marín. 2021. Detection of four RNA viruses in commercial and informal potato seed tubers in Antioquia (Colombia). Archives of Phytopathology and Plant Protection 54(5-6): 273-294. https:// 10.1080/03235408.2020.1829424. Singh, R.P., J. Kurz, G. Boiteau, G. Bernard. 1995. Detection of potato leafroll virus in single aphids by the reverse transcription polymerase chain reaction and its potential epidemiological application. Journal of Virological Methods 1: 133–143. https://10.1016/0166-0934(95)00056-z Singh M, Singh RP, Fageria MS, Nie X, Coffin R, Hawkins G. 2013. Optimization of a Real-Time RT-PCR assay and its comparison with ELISA, conventional RT-PCR and the grow-out test for large scale diagnosis of Potato virus Y in dormant potato tubers. American Journal of Potato Research 90(1):43–50. https://doi.org/10.1007/s12230-012-9274-z Stammler, J., A. Oberneder, A. Kellermann, J. Hadersdorfer. 2018. Detecting potato viruses using direct reverse transcription quantitative PCR (DiRT-qPCR) without RNA purification: an alternative to DAS-ELISA. European Journal of Plant Pathology 152: 237–248. https:// 10.1007/s10658-018-1468-x Whitworth, J.L., P.B. Hamm, P. Nolte. 2012. Distribution of Potato virus Y strains in tubers during the postharvest period. American Journal of Potato Research 89(2): 136–141. https:// 10.1007/s12230-012-9235-6 Whitworth, J.L., S.M. Gray, J.T. Ingram, D.G. Hall. 2021. Foliar and tuber symptoms of U.S. potato varieties to multiple strains and isolates of potato virus Y. American Journal of Potato Research 98: 93–103. https://10.1007/s12230-020-09820-1 Agindotan, B. O., Shiel, P. J. y Berger, P. H. (2007). Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan® real-time RT-PCR. Journal of Virological Methods, 142(1–2), 1–9. https://doi.org/10.1016/j.jviromet.2006.12.012 Álvarez, D., Gutiérrez, P. y Marín, M. (2016). Caracterización molecular del Potato virus V (PVV) infectando Solanum phureja mediante secuenciación de nueva generación. Acta Biológica Colombiana, 21(3), 521-531. https://doi.org/10.15446/abc.v21n3.54712 Álvarez-Yepes, D., Gutiérrez-Sánchez, P. y Marín-Montoya, M. (2017). Secuenciación del genoma del Potato yellow vein virus (PYVV) y desarrollo de una prueba molecular para su detección. Bioagro, 29(1), 3-14. Antonova, O., Apalikova, O., Ukhatova, Y., Krylova, E., Shuvalov, O., Shuvalova, A. R. y Gavrilenko, T. A. (2017). Eradication of viruses in microplants of three cultivated potato species (Solanum tuberosum L., S. Phureja Juz. & Buk., S. stenotomum Juz. & Buk.) using combined thermo-chemotherapy method. Sel'skokhozyaistvennaya Biologiya, 52, 95-104. https://doi.org/10.15389/agrobiology.2017.1.95eng. Bamberg, J., Martin, M., Abad, J., Jenderek, M., Tanner, J., Donnelly, D., Nassar, A., Veilleux, R. y Novy, R. (2016). In vitro technology at the US potato Genebank. In Vitro Cellular and Developmental Biology - Plant, 52, 213-225. https://doi.org/10.1007/s11627-016-9753-x Bettoni, J. C., Mathew, L., Pathirana, R., Wiedow, C., Hunter, D., McLachlan, A., Khan, S., Tang, J. y Nadarajan, J. (2022). Eradication of Potato Virus S, Potato Virus A, and Potato Virus M From Infected in vitro-grown potato shoots using in vitro therapies. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.878733 Chavez-Barrantes, N. y Gutiérrez-Soto, M. (2017). Respuestas al estrés por calor en los cultivos. II. Tolerancia y tratamiento agronómico. Agronomía Mesoamericana, 28(1), 255 - 271. https://doi.org/10.15517/am.v28i1.21904 Daurov, D., Daurova, A., Karimov, A., Tolegenova, D., Volkov, D., Raimbek, D., Zhambakin, K. y Shamekova, M. (2020). Determining Effective Methods of Obtaining Virus-Free Potato for Cultivation in Kazakhstan. American Journal of Potato Research, 97, 367–375. https://doi.org/10.1007/s12230-020-09787-z Dawson, W. O. y Lozoya, S. H. (1984). Examination of the mode of action of ribavirin against tobacco mosaic virus. Intervirology., 22(2), 77–84. https://doi.org/10.1159/000149537 Ehsanpour, A. y Jones, M. (2001). Plant regeneration from mesophyll protoplasts of potato (Solanum tuberosum L.) cultivar delaware using silver thiosulfate (STS). Journal of sciences, 12, 103-110. Faccioli, G. y Colalongo, M. (2002). Eradication of potato virus Y and potato leafroll virus by chemotherapy of infected potato stem cuttings. Phytopathologia Mediterranea, 41, 76-78. FAOSTAT. (2018). Food and agriculture data. Recuperado el 10 de septiembre de 2020 de http://www.fao.org/faostat/en/#home Gallo, Y., Sierra, A., Marín, M. y Gutiérrez, P. A. (2021). Prevalencia de cinco virus de ARN en tubérculos-semilla de papa cultivados en Antioquia (Colombi9a). Biotecnología en el Sector Agropecuario y Agroindustrial, 19(1), 66-78. https://dx.doi.org/10.18684 García, A.; Higuita, M.; Hoyos, R.; Gallo, Y.; Marín, M. y Gutiérrez, P. (2021). Prevalence of RNA viruses in certified, and informal potato seed tubers in the province of Antioquia (Colombia). Crop Prot; código del registro: No.: CROPRO-D-21-01027. Guzmán, M., Román, V., Franco, X. y Rodríguez, P. (2010). Presencia de cuatro virus en algunas accesiones de la Colección Central Colombiana de papa mantenida en campo. Agronomía Colombiana, 28(2), 225–233. Hoque, M. (2010). In Vitro Regeneration Potentiality of Potato under Different Hormonal Combination. World Journal of Agricultural Sciences, 6(6), 660-663. http://www.idosi.org/wjas/wjas6(6)/5.pdf Kaiser, W. (1980). Use of Thermotherapy to Free Potato Tubers of Alfalfa mosaic, Potato leaf roll, and Tomato black ring viruses. Phytopathology, 70(11), 1119. https://doi.org/10.1094/phyto-70-1119 Mumford, R. A., Walsh, K., Barker, I. y Boonham, N. (2000). Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. Phytopathology, 90(5), 448-453. https://doi.org/10.1094/PHYTO.2000.90.5.448 Murashige, T. y Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 474-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x Muthoni, J., Shimelis, H. y Melis, R. (2013). Potato production in Kenya: Farming systems and production constraints. The Journal of Agricultural Science, 5(5), 182-197. https://doi.org/10.5539/jas.v5n5p182 Nasir, I., Tabassum, B., Latif, Z., Javed M., Haider, M. Javed, M. y Husnain, T. (2010). Strategies to control Potato virus Y under in vitro conditions. Pakistan Journal of Phytopathology, 22(1), 63-70. Salazar, L. F. (1996). Potato viruses and their control. Lima: International Potato Center. Savenkov, E. I., Sandgren, M. y Valkonen, J. P. T. (1999). Complete sequence of RNA 1 and the presence of tRNA like structures in all RNAs of Potato mop-top virus, genus Pomovirus. Journal of General Virology, 80(10), 2779-2784. https://doi.org/10.1099/0022-1317-80-10-2779 Sherwood, J. L. (1994). Virus free-plants. Dixon, R. A. y Gonzales R. A. (Ed). Plant Cell Culture. A practical approach. (2a ed). IRL Press. UK. (pp. 135-138). Shoala, T., Eid, Kh. E. y El-fiki, I. A. I. (2019). Impact of chemotherapy and thermotherapy treatments on the presence of potato viruses PVY, PVX and PLRV in tissue-cultured shoot tip meristem. Journal of Plant Protection and Pathology, 10(12), 581-585. Simpkins, I.; Walkey, D. G. A. y Neely, H. A. (1981). Chemical suppression of virus in cultured plant tissues. Annals Applied Biology, 99(2), 161–169.https://doi.org/10.1111/j.1744-7348.1981.tb05143.x Singh, R. P., Kurz, J., Boiteau, G. y Bernard, G. (1995). Detection of Potato leafroll virus in single aphids by the reverse transcription polymerase chain reaction and its potencial epidemiological application. Journal of Virological Methods, 1, 133–143. https://10.1016/0166-0934(95)00056-z Wagoire, W. W., Kakuhenzire, R., Kashaija, I. N., Lemaga, B., Demo, P. y Kimmone, G. (2005). Seed potato production in Uganda: Current status and future prospects. African Crop Science Conference Proceedings, 7, 739-743. Waswa, M.; Kakuhenzire, R. y Ochwo-Ssemakula, M. (2017). Effect of thermotherapy duration, virus type and cultivar interactions on elimination of potato viruses X and S in infected seed stocks. African Journal of Plant Science. 11(3):61-70. https://doi.org/10.5897/AJPS2016.1497 Yang, L., Nie, B., Liu, J. y Song, B. (2014). A Reexamination of the effectiveness of ribavirin on eradication of viruses in potato plantlets in vitro using ELISA and quantitative RT-PCR. American Journal of Potato Research, 91(3), 304-311. https://doi.org/10.1007/s12230-013-9350 |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Hoyos Sánchez, Rodrigo Alberto6ddb39f31a5436d96ecc1e671ae2131e600Gutiérrez Sánchez, Pablo Andrés03fa1cb1dfa744c01ad629ca741ddd94García Torres, Andrea Stefania2ce89275edf36b2b46e291a75276129aMarìn Montoya, Mauricio AlejandroCorrea Londoño, Guillermo AntonioBiotecnología MicrobianaBiotecnología Vegetal Unalmed Cib2022-09-02T21:32:26Z2022-09-02T21:32:26Z2022-08-20https://repositorio.unal.edu.co/handle/unal/82244Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, tablasEn Colombia, el cultivo de papa (Solanum tuberosum y S. phureja) es uno de los renglones agrícolas más importantes para la zona Andina. Con el fin de aportar nuevos elementos al conocimiento de la situación virológica de la papa en el país, y especialmente en Antioquia, en este proyecto se utilizaron diversas técnicas moleculares (RT-PCR, qPCR, secuenciación Sanger y HTS) para el diagnóstico, identificación, y caracterización genómica de los virus presentes en el material de siembra certificado y no certificado de los cultivares Diacol Capiro y Criolla Colombia, comercializados en Antioquia y en menor proporción en la sabana Cundiboyacense. Los resultados indicaron la presencia de los virus de RNA: virus Y de la papa (Potato virus Y - PVY), virus S de la papa (Potato virus S - PVS), virus X de la papa (Potato virus X - PVX), virus del amarillamiento de las venas de la papa (Potato yellow vein virus - PYVV), virus del enrollamiento de la hoja de papa (Potato leafroll virus - PLRV), virus mop-top de la papa (Potato mop-top virus - PMTV), virus V de la papa (Potato virus V- PVV) y virus B de la papa (Potato virus B - PVB), en ambos cultivares de papa y en ambos tipos de material de siembra. En las evaluaciones directas sobre tubérculos obtenidos en la sabana Cundiboyacense, se detectaron siete (PVY, PVX, PVS, PLRV, PYVV, PMTV y PVB) de los ocho virus en material de siembra certificado de cv. Diacol Capiro, mientras que, en los tubérculos no certificados, se encontraron seis de los virus (PVY, PVX, PVS, PYVV, PLRV y PMTV). Para el caso de Antioquia, las evaluaciones se dividieron en las zonas del oriente y norte del departamento. En el oriente, se detectaron los ocho virus en ambos cultivares; en cuánto al norte, se encontraron siete de los virus (PVY, PVS, PVX, PLRV, PYVV, PMTV y PVB) en el cv. Diacol Capiro. Para el material del cv. Criolla Colombia, no fue posible obtener material certificado en la zona del norte, pero si se detectaron los ocho virus en los tubérculos no certificados. Utilizando la secuenciación masiva de alto rendimiento, se aumentó el número de secuencias disponibles de genomas de los virus PVY, PVV, PVX, PVS, PMTV y PVB, además se realizó el primer reporte de una secuencia de PVV infectando el cv. Diacol Capiro en Colombia y el primer registro del virus D de la necrosis del tabaco (Tobacco necrosis virus D - TNV-D) en el país. Adicionalmente, se realizó un ensayo piloto sobre la distribución viral en brotes de diferentes tubérculos pertenecientes a un mismo lote de semilla, encontrándose que para alcanzar una precisión del 90% en la detección viral, es necesario evaluar de manera independiente al menos tres tubérculos por lote y de dos a tres brotes por tubérculo. Por último, se evaluó el efecto de terapias in vitro para la eliminación viral en plántulas ambos cultivares, evidenciándose una reducción en la proporción de infección en vitroplantas sometidas a quimioterapia por 45 días con respeto a los tratamientos control, de los virus PVY, PVS, PVX, PLRV y PYVV en todas las dosis de ribavirina (50, 75 y 100 ppm); asimismo en las evaluaciones de termoterapia se registraron disminuciones en los virus PVY, PYVV y PMTV en las plántulas sometidas a 35°C, PVV, PVS, PYVV, PMTV y PVX en 36°C, PVS, PVX, PMTV y PYVV en 37°C, y PVS y PLRV en vitroplantas a 38°C por dos semanas. Se espera que los resultados obtenidos en esta tesis se constituyan en un aporte importante para el apoyo de los programas de manejo integrado de las enfermedades virales en papa. (Texto tomado de la fuente)In Colombia, potato (Solanum tuberosum y S. phureja) is one of the most important staple food crops in the Andean region. This work was designed to update our current knowledge of the potato virome in Colombia using a wide range of molecular tools (RT-PCR, qPCR, Sanger and HTS) for the diagnostics, identification and genomic characterization of the viruses present in certified and uncertified planting material used by potato farmers in the province of Antioquia, and some regions of Cundinamarca and Boyacá. The results presented here, reveal a high prevalence of the RNA viruses Potato virus Y (PVY), Potato virus S (PVS), Potato virus X (PVX), Potato yellow vein virus (PYVV), Potato leafroll virus (PLRV), Potato mop-top virus (PMTV), Potato virus V (PVV) and Potato virus B (PVB) in planting material from both cultivars. Direct testing on seed-tubers produced in the highlands of Boyacá and Cundinamarca revealed widespread infection with PVY, PVX, PVS, PLRV, PYVV, PMTV, and PVB in certified cv. Diacol Capiro seeds, and PVY, PVX, PVS, PYVV, PLRV, and PMTV, in uncertified ones. In eastern Antioquia, this investigation detected all eight viruses infecting both cultivars. As for northern Antioquia, seven viruses (PVY, PVS, PVX, PLRV, PYVV, PMTV y PVB) were found in cv. Diacol Capiro. All eight viruses under study were identified in uncertified Criolla Colombia seeds, unfortunately, certified seeds for this cultivar were not available in northern Antioquia at the time of study. HTS allowed either the partial or complete genome characterization of new PVY, PVV, PVX, PVS, PMTV and PVB isolates, in addition to the first PVV isolate infecting S. tuberosum in Colombia, and the first report for Tobacco necrosis virus D - TNV-D. A pilot study on the distribution of viruses across sprouts within the same tuber was performed revealing that to achieve 90% accuracy it is necessary to test a minimum of two or three sprouts from three different tubers per lot. Finally, this work also tested the effect of chemotherapy with ribavirin at 50, 75, and 100 ppm for 45 days; and thermotherapy at 35, 36, 37 and 38°C for 15 days, in reducing the proportion of vitroplants infected with PVY, PVV, PVS, PVX, PYVV, PLRV and PMTV in potato cultivars Diacol Capiro and Criolla Colombia. Chemotherapy proved to be effective in reducing the proportion of PVY, PVS, PVX, PLRV y PYVV at all ribavirin concentrations (50, 75 and 100 ppm). Additionally, thermotherapy reduced the proportion of vitroplants infected with PVY, PYVV and PMTV at 35°C, PVV, PVS, PYVV, PMTV and PVX at 36°C, PVS, PVX, PMTV and PYVV at 37°C, and PVS and PLRV at 38°C. These results are expected to be an important contribution in support of viral disease management programs of potato in Colombia.Esta tesis fue financiada por el Fondo de Ciencia, Tecnología e Innovación del Sistema General de Regalías del Departamento de Antioquia (Colombia) (Convenio No. 4600007658–779), a través del Proyecto: "Desarrollo de una plataforma molecular y bioinformática para el diagnóstico de virus en cultivos y material de siembra de papa (Solanum tuberosum y S. phureja) en Antioquia" (Código: 1101-805-62787), ejecutado por la Universidad Nacional de Colombia sede Medellín, Universidad CES y Fedepapa. El proyecto fue supervisado por la Secretaria de Agricultura y Desarrollo Rural de Antioquia y por el Ministerio de Ciencia, Tecnología e Innovación de Colombia. 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