Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems
We evaluated a technique of micropropagation in a thornless variety of blackberry (Rubus glaucus) from shoot apical meristems. In the establishment phase, we evaluated a disinfection protocol: soapy solution (commercial detergent and water) for 5 minutes + 70% alcohol for 2 minutes + 3% hypochlorite...
- Autores:
-
Sigarroa Rieche, Alina Katil
Lucía, Claudia
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2011
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/39996
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/39996
http://bdigital.unal.edu.co/30093/
- Palabra clave:
- 6 Tecnología (ciencias aplicadas) / Technology
63 Agricultura y tecnologías relacionadas / Agriculture
Cultivo de meristemas
cultivo in vitro
micropropagación
mora sin espinas
Rubus glaucus
In vitro cultivation
meristems
micropropagation
Rubus glaucus
thornless blackberry
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems |
| title |
Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems |
| spellingShingle |
Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems 6 Tecnología (ciencias aplicadas) / Technology 63 Agricultura y tecnologías relacionadas / Agriculture Cultivo de meristemas cultivo in vitro micropropagación mora sin espinas Rubus glaucus In vitro cultivation meristems micropropagation Rubus glaucus thornless blackberry |
| title_short |
Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems |
| title_full |
Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems |
| title_fullStr |
Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems |
| title_full_unstemmed |
Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems |
| title_sort |
Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems |
| dc.creator.fl_str_mv |
Sigarroa Rieche, Alina Katil Lucía, Claudia |
| dc.contributor.author.spa.fl_str_mv |
Sigarroa Rieche, Alina Katil Lucía, Claudia |
| dc.subject.ddc.spa.fl_str_mv |
6 Tecnología (ciencias aplicadas) / Technology 63 Agricultura y tecnologías relacionadas / Agriculture |
| topic |
6 Tecnología (ciencias aplicadas) / Technology 63 Agricultura y tecnologías relacionadas / Agriculture Cultivo de meristemas cultivo in vitro micropropagación mora sin espinas Rubus glaucus In vitro cultivation meristems micropropagation Rubus glaucus thornless blackberry |
| dc.subject.proposal.spa.fl_str_mv |
Cultivo de meristemas cultivo in vitro micropropagación mora sin espinas Rubus glaucus In vitro cultivation meristems micropropagation Rubus glaucus thornless blackberry |
| description |
We evaluated a technique of micropropagation in a thornless variety of blackberry (Rubus glaucus) from shoot apical meristems. In the establishment phase, we evaluated a disinfection protocol: soapy solution (commercial detergent and water) for 5 minutes + 70% alcohol for 2 minutes + 3% hypochlorite with two different exposure times: T1 for 5 minutes and T2 for 10 minutes. While the microcuttings were disinfected, the meristematic shoots were removed and established in vitro in a completely random design to evaluate two cultivation mediums: M1 and M2. From the developed seedlings, the multiplication was performed in three cultivation mediums: M1, M3 and M4. The two disinfection treatments were 100% effective on each of the explants. The planted apex meristem allowed the establishment of aseptic cultivation and adequate development of the explants after six weeks of cultivation with rates of germination of 83.4% for M1 and 66.6% for M2. The analysis of variance (ANOVA) and multiple range test with Fisher's method (LSD), showed that M1 medium favored multiplication with a better growth and development of the explant with a multiplication coefficient of 7.5 shoots per seedling, and an average height of 1.95 cm. |
| publishDate |
2011 |
| dc.date.issued.spa.fl_str_mv |
2011-10-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-06-28T09:27:44Z |
| dc.date.available.spa.fl_str_mv |
2019-06-28T09:27:44Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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publishedVersion |
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ISSN: 2323-0118 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/39996 |
| dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/30093/ |
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ISSN: 2323-0118 |
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https://repositorio.unal.edu.co/handle/unal/39996 http://bdigital.unal.edu.co/30093/ |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.spa.fl_str_mv |
http://revistas.unal.edu.co/index.php/acta_agronomica/article/view/28849 |
| dc.relation.ispartof.spa.fl_str_mv |
Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica Acta Agronómica |
| dc.relation.references.spa.fl_str_mv |
Sigarroa Rieche, Alina Katil and Lucía, Claudia (2011) Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems. Acta Agronómica, 60 (4). pp. 347-354. ISSN 2323-0118 |
| dc.rights.spa.fl_str_mv |
Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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Universidad Nacional de Colombia Sede Palmira |
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Sigarroa Rieche, Alina Katil98c18652-6fe0-49a4-9b7f-037b8e33263c300Lucía, Claudia02352eed-48c3-45b5-8646-e45f0d549ccd3002019-06-28T09:27:44Z2019-06-28T09:27:44Z2011-10-01ISSN: 2323-0118https://repositorio.unal.edu.co/handle/unal/39996http://bdigital.unal.edu.co/30093/We evaluated a technique of micropropagation in a thornless variety of blackberry (Rubus glaucus) from shoot apical meristems. In the establishment phase, we evaluated a disinfection protocol: soapy solution (commercial detergent and water) for 5 minutes + 70% alcohol for 2 minutes + 3% hypochlorite with two different exposure times: T1 for 5 minutes and T2 for 10 minutes. While the microcuttings were disinfected, the meristematic shoots were removed and established in vitro in a completely random design to evaluate two cultivation mediums: M1 and M2. From the developed seedlings, the multiplication was performed in three cultivation mediums: M1, M3 and M4. The two disinfection treatments were 100% effective on each of the explants. The planted apex meristem allowed the establishment of aseptic cultivation and adequate development of the explants after six weeks of cultivation with rates of germination of 83.4% for M1 and 66.6% for M2. The analysis of variance (ANOVA) and multiple range test with Fisher's method (LSD), showed that M1 medium favored multiplication with a better growth and development of the explant with a multiplication coefficient of 7.5 shoots per seedling, and an average height of 1.95 cm.Se evaluó una técnica de micropropagación de plantas de mora (Rubus glaucus) de la variedad sin espinas, a partir de ápices meristemáticos. En la fase de establecimiento se evaluó un protocolo de desinfección utilizando por 5 min solución de jabón detergente comercial y agua + alcohol 70% por 2 min + hipoclorito 3% con dos tiempos de exposición diferentes: T1 por 5 min y T2 por 10 min. Después de desinfectar las microestacas se extrajeron los ápices meristemáticos y se establecieron in vitro bajo un diseño completamente al azar para evaluar dos medios de cultivo: M1 y M2. A partir de las plántulas desarrolladas se efectuó la multiplicación en los medios de cultivo M1, M3 y M4. Ambos tratamientos de desinfección resultaron efectivos alcanzando 100% de desinfección de los explantes con cada uno de ellos. La siembra de ápices meristemáticos permitió el establecimiento de cultivos asépticos y un adecuado desarrollo de los explantes después de seis semanas de cultivo, con prendimiento de 83.4% para M1 y 66.6% para M2. El análisis de varianza (Anova) y la prueba de rangos múltiples mostraron que la multiplicación fue mejor en el medio M1 con una mayor tasa de crecimiento y desarrollo del explante, al obtener coeficientes de multiplicación de 7.5 brotes/plántula y una altura promedio de 1.95 cm.application/pdfspaUniversidad Nacional de Colombia Sede Palmirahttp://revistas.unal.edu.co/index.php/acta_agronomica/article/view/28849Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaSigarroa Rieche, Alina Katil and Lucía, Claudia (2011) Establishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristems. Acta Agronómica, 60 (4). pp. 347-354. ISSN 2323-01186 Tecnología (ciencias aplicadas) / Technology63 Agricultura y tecnologías relacionadas / AgricultureCultivo de meristemascultivo in vitromicropropagaciónmora sin espinasRubus glaucusIn vitro cultivationmeristemsmicropropagationRubus glaucusthornless blackberryEstablishment and in vitro multiplication of thornless blackberry (rubus glaucus benth.) by shoot apical meristemsArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL28849-103451-2-PB.pdfapplication/pdf352117https://repositorio.unal.edu.co/bitstream/unal/39996/1/28849-103451-2-PB.pdfd801097026dbe561ab006adcf4bd6431MD51THUMBNAIL28849-103451-2-PB.pdf.jpg28849-103451-2-PB.pdf.jpgGenerated Thumbnailimage/jpeg7939https://repositorio.unal.edu.co/bitstream/unal/39996/2/28849-103451-2-PB.pdf.jpg1e1198f232e5b8af3cd6b24b079758ceMD52unal/39996oai:repositorio.unal.edu.co:unal/399962023-01-26 23:05:12.277Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |