Evaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneas

ilustraciones, fotografías, gráficas, tablas

Autores:
Vargas Carreño, Elga Johanna
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2022
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/82294
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/82294
https://repositorio.unal.edu.co/
Palabra clave:
610 - Medicina y salud::616 - Enfermedades
Cicatrización de Heridas
Células Madre Mesenquimatosas
Neovascularización Fisiológica
Wound Healing
Mesenchymal Stem Cells
Neovascularization, Physiologic
Células madre
Células madre mesenquimales
Medio condicionado
Angiogénesis
Reparación de heridas
Mesenchymal stem cells
Stromal cells
Exvivo expansion
Scale-up
Conditioned media
Microcarriers
Wound healing
Rights
openAccess
License
Atribución-NoComercial-CompartirIgual 4.0 Internacional
id UNACIONAL2_6215a763927f6cc00522f9289b2a5bb7
oai_identifier_str oai:repositorio.unal.edu.co:unal/82294
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Evaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneas
dc.title.translated.eng.fl_str_mv Evaluation of the angiogenic potential of conditioned media from mesenquimal stem cells derived from adipose tissue cultivated in a dynamic system in the healing of cutaneous wounds
title Evaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneas
spellingShingle Evaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneas
610 - Medicina y salud::616 - Enfermedades
Cicatrización de Heridas
Células Madre Mesenquimatosas
Neovascularización Fisiológica
Wound Healing
Mesenchymal Stem Cells
Neovascularization, Physiologic
Células madre
Células madre mesenquimales
Medio condicionado
Angiogénesis
Reparación de heridas
Mesenchymal stem cells
Stromal cells
Exvivo expansion
Scale-up
Conditioned media
Microcarriers
Wound healing
title_short Evaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneas
title_full Evaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneas
title_fullStr Evaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneas
title_full_unstemmed Evaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneas
title_sort Evaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneas
dc.creator.fl_str_mv Vargas Carreño, Elga Johanna
dc.contributor.advisor.spa.fl_str_mv Chaparro Garzón, Orlando
dc.contributor.author.spa.fl_str_mv Vargas Carreño, Elga Johanna
dc.contributor.researchgroup.spa.fl_str_mv Biología de Células Madre
dc.subject.ddc.spa.fl_str_mv 610 - Medicina y salud::616 - Enfermedades
topic 610 - Medicina y salud::616 - Enfermedades
Cicatrización de Heridas
Células Madre Mesenquimatosas
Neovascularización Fisiológica
Wound Healing
Mesenchymal Stem Cells
Neovascularization, Physiologic
Células madre
Células madre mesenquimales
Medio condicionado
Angiogénesis
Reparación de heridas
Mesenchymal stem cells
Stromal cells
Exvivo expansion
Scale-up
Conditioned media
Microcarriers
Wound healing
dc.subject.decs.spa.fl_str_mv Cicatrización de Heridas
Células Madre Mesenquimatosas
Neovascularización Fisiológica
dc.subject.decs.eng.fl_str_mv Wound Healing
Mesenchymal Stem Cells
Neovascularization, Physiologic
dc.subject.proposal.spa.fl_str_mv Células madre
Células madre mesenquimales
Medio condicionado
Angiogénesis
Reparación de heridas
dc.subject.proposal.eng.fl_str_mv Mesenchymal stem cells
Stromal cells
Exvivo expansion
Scale-up
Conditioned media
Microcarriers
Wound healing
description ilustraciones, fotografías, gráficas, tablas
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-09-16T12:34:39Z
dc.date.available.none.fl_str_mv 2022-09-16T12:34:39Z
dc.date.issued.none.fl_str_mv 2022-04
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/doctoralThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_db06
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TD
format http://purl.org/coar/resource_type/c_db06
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/82294
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/82294
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.indexed.spa.fl_str_mv Bireme
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spelling Atribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Chaparro Garzón, Orlando01a9c792304abaab8fb28a2e998aa841Vargas Carreño, Elga Johanna532fee48a51d26c9bcc3a05ccbf7739a600Biología de Células Madre2022-09-16T12:34:39Z2022-09-16T12:34:39Z2022-04https://repositorio.unal.edu.co/handle/unal/82294Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, gráficas, tablasIntroducción Las células madre mesenquimales derivadas de tejido adiposo (A-MSC) ejercen su acción regenerativa-reparativa mediante un mecanismo de acción paracrino producido por cientos de factores tróficos, componentes de la matriz extracelular, vesículas extracelulares, etc., que son secretados al medio extracelular. Este secretoma puede recolectarse desde los medios de cultivo como medios condicionados (MC). Los MC de A-MSC poseen un efecto tisular similar al trasplante celular, lo cual permite su uso como alternativa terapéutica. Hasta el momento, los MC de A-MSC son obtenidos esencialmente en cultivos de dos dimensiones (2D) estáticos en normoxia, sin embargo, para escalar su producción es necesario realizar el cultivo en sistemas dinámicos garantizando la conservación de su potencial regenerativo-reparativo. El objetivo principal de este trabajo fue evaluar si al escalar la producción de los MC introduciendo dos cambios en el sistema de cultivo (un sistema tridimensional dinámico e hipoxia) se mantiene la capacidad angiogénica y funcional como una medida indirecta de la conservación de su potencial terapéutico. Materiales y Métodos Se obtuvieron MC de A-MSC de células cultivadas en un sistema 2D estático y un sistema tridimensional (3D) dinámico que emplea spinners flasks acoplados a microtrasportadores, tanto en condiciones de normoxia como de hipoxia. Posteriormente se realizó la cuantificación y la caracterización de su composición proteica y se evaluó su potencial de angiogénesis in vitro y su capacidad funcional mediante un ensayo in vivo de curación de heridas cutáneas agudas en ratas. Resultados Los A-MSC cultivadas en el sistema 3D dinámico conservan su inmunofenotipo y su capacidad de diferenciación multilinaje. Los MC obtenidos de estas A-MSC presentan un perfil proteico único dependiente del donante y modificado por las nuevas variables introducidas al cultivo. Los MC obtenidos en el sistema 3D dinámico conservan su potencial angiogénico y su capacidad funcional demostrado mediante 3 ensayos in vitro de angiogénesis y un modelo in vivo de curación de heridas excisionales agudas en la piel de ratas. Conclusiones Es posible escalar la producción de los MC de A-MSC garantizando la conservación de su potencial angiogénico y su capacidad funcional. La obtención de los MC en condiciones 3D dinámicas en hipoxia puede potenciar su efecto terapéutico en el cierre de heridas cutáneas agudas. (Texto tomado de la fuente).Introduction Mesenchymal stem cells derived from adipose tissue (A-MSC) perform their regenerative-reparative process through a paracrine action mechanism exerted by hundreds of trophic factors, components of the extracellular matrix, extracellular vesicles, etc., which are secreted into the extracellular medium. This secretome can be gathered from culture media as conditioned media (CM). MC of A-MSC have a tissue effect similar to cell transplantation, which allows their use as a therapeutic alternative. Until now, the MC of A-MSC is essentially obtained in static two-dimensional cultures in normoxia, however, to scale the production of MC, it´s necessary to proceed with the culture A-MSC in dynamic systems guaranteeing the conservation of their regenerative-reparative potential. The main objective of this work was to evaluate if when scaling the production of MC of A-MSC, introducing two changes in the culture system (a dynamic three-dimensional system and hypoxia), the angiogenic and functional capacity keeps up as an indirect measure of its therapeutic conservation potential. Materials and methods MC of A-MSC were obtained from cells that were cultured in the traditional static two-dimensional system and a dynamic three-dimensional system employing spinner flasks coupled to microcarriers, under both normoxic and hypoxic conditions. Subsequently, the quantification and characterization of the protein composition of the MC was performed and their in vitro angiogenesis potential and functional capacity were evaluated by means of an in vivo acute cutaneous wound healing assay. Results A-MSCs cultured in the dynamic three-dimensional system retains their immunophenotype and multilineage differentiation capacity. The MC obtained from these A-MSC shows a unique protein profile dependent on the donor and modified by the new variables introduced into the culture. The CM obtained in the dynamic three-dimensional system retain their angiogenic potential and their functional capacity demonstrated by 3 in vitro angiogenesis assays and an in vivo model of acute excisional wound healing in rat skin. Conclusions It’s possible to scale the production of MCs from A-MSC, guaranteeing the conservation of their angiogenic potential and their functional capacity, using a dynamic three-dimensional system (spinner flasks coupled to microcarriers). Obtaining MC under dynamic hypoxic conditions can enhance their therapeutic effect in the closure of acute skin wounds.Incluye anexosDoctoradoDoctor en Ciencias BiomédicasMesenchymal Stem Cellsxiii, 257 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina - Doctorado en Ciencias BiomédicasFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::616 - EnfermedadesCicatrización de HeridasCélulas Madre MesenquimatosasNeovascularización FisiológicaWound HealingMesenchymal Stem CellsNeovascularization, PhysiologicCélulas madreCélulas madre mesenquimalesMedio condicionadoAngiogénesisReparación de heridasMesenchymal stem cellsStromal cellsExvivo expansionScale-upConditioned mediaMicrocarriersWound healingEvaluación del potencial angiogénico de medios condicionados obtenidos de células madre mesenquimales derivadas de tejido adiposo cultivadas en un sistema dinámico en la curación de heridas cutáneasEvaluation of the angiogenic potential of conditioned media from mesenquimal stem cells derived from adipose tissue cultivated in a dynamic system in the healing of cutaneous woundsTrabajo de grado - Doctoradoinfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_db06Texthttp://purl.org/redcol/resource_type/TDBireme1. 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Int Sch Res Noticies. 2014: 652713. doi: 10.1155/2014/652713.MinCienciasEstudiantesInvestigadoresMaestrosProveedores de ayuda financiera para estudiantesReceptores de fondos federales y solicitantesResponsables políticosLICENSElicense.txtlicense.txttext/plain; charset=utf-84675https://repositorio.unal.edu.co/bitstream/unal/82294/3/license.txtb577153cc0e11f0aeb5fc5005dc82d8aMD53ORIGINAL2949741259-2022.pdf2949741259-2022.pdfTesis de Doctorado en Ciencias Biomédicasapplication/pdf11140935https://repositorio.unal.edu.co/bitstream/unal/82294/4/2949741259-2022.pdf0be1245dae25204ff3c9c0e2ac153c84MD54THUMBNAIL2949741259-2022.pdf.jpg2949741259-2022.pdf.jpgGenerated Thumbnailimage/jpeg4871https://repositorio.unal.edu.co/bitstream/unal/82294/5/2949741259-2022.pdf.jpg160d29c8d8a4c06269c07fe7a47f1918MD55unal/82294oai:repositorio.unal.edu.co:unal/822942024-08-11 01:07:54.699Repositorio Institucional Universidad Nacional de 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