Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development
Theobroma cacao L. and its products are consumed worldwide. Those products are of great research interest due to antioxidant properties of some of their polyphenolic constituents. The amount of these polyphenols and polysaccharides has shown that can interfere with the high quality and quantity of n...
- Autores:
-
Ramírez, María Henao
Salazar Duque, Héctor Jaime
Urrea Trujillo, Aura Ines
- Tipo de recurso:
- Article of journal
- Fecha de publicación:
- 2018
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/68101
- Acceso en línea:
- https://repositorio.unal.edu.co/handle/unal/68101
http://bdigital.unal.edu.co/69134/
- Palabra clave:
- 55 Ciencias de la tierra / Earth sciences and geology
63 Agricultura y tecnologías relacionadas / Agriculture
DNA isolation
field cocoa plants
in vitro cocoa plantlets
leaf development
PCR
SSRs
Aislamiento de ADN
plantas de cacao de campo
plántulas de cacao in vitro
desarrollo de hojas
PCR
SSRs
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development |
| title |
Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development |
| spellingShingle |
Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development 55 Ciencias de la tierra / Earth sciences and geology 63 Agricultura y tecnologías relacionadas / Agriculture DNA isolation field cocoa plants in vitro cocoa plantlets leaf development PCR SSRs Aislamiento de ADN plantas de cacao de campo plántulas de cacao in vitro desarrollo de hojas PCR SSRs |
| title_short |
Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development |
| title_full |
Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development |
| title_fullStr |
Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development |
| title_full_unstemmed |
Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development |
| title_sort |
Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development |
| dc.creator.fl_str_mv |
Ramírez, María Henao Salazar Duque, Héctor Jaime Urrea Trujillo, Aura Ines |
| dc.contributor.author.spa.fl_str_mv |
Ramírez, María Henao Salazar Duque, Héctor Jaime Urrea Trujillo, Aura Ines |
| dc.subject.ddc.spa.fl_str_mv |
55 Ciencias de la tierra / Earth sciences and geology 63 Agricultura y tecnologías relacionadas / Agriculture |
| topic |
55 Ciencias de la tierra / Earth sciences and geology 63 Agricultura y tecnologías relacionadas / Agriculture DNA isolation field cocoa plants in vitro cocoa plantlets leaf development PCR SSRs Aislamiento de ADN plantas de cacao de campo plántulas de cacao in vitro desarrollo de hojas PCR SSRs |
| dc.subject.proposal.spa.fl_str_mv |
DNA isolation field cocoa plants in vitro cocoa plantlets leaf development PCR SSRs Aislamiento de ADN plantas de cacao de campo plántulas de cacao in vitro desarrollo de hojas PCR SSRs |
| description |
Theobroma cacao L. and its products are consumed worldwide. Those products are of great research interest due to antioxidant properties of some of their polyphenolic constituents. The amount of these polyphenols and polysaccharides has shown that can interfere with the high quality and quantity of nucleic acids for molecular research. Therefore, cocoa DNA extraction protocols can require a large amount of plant material and optimization time according with plant material source. The aim of this study was to evaluate the quality and quantity of DNA isolated from field plant leaves at different developmental stages from TSH565 genotype using different DNA extraction protocols. In addition, DNA extraction protocol was evaluated for small amount of young leaf tissue collected from in vitro plantlets from CCN51 and TSH565 genotype. Subsequently, the selectivity of different polymerase enzymes for PCR amplification using the obtained DNA was evaluated. This study revealed that D stage of development in field leaves was efficient for extraction of high-quality genomic DNA using the PowerPlant® Pro Kit modified (183.80 ng.µL-1 (1.98 A260/A280-1.98 A260/A230)). Highest DNA concentrations were obtained for FPL with 128.68 ng.µL-1 and 114.42 ng.µL-1 for CCN51 and TSH565 respectively and with IVL, which was obtained 54.24 ng.µL-1 for CCN51 and 56.52 ng.µL-1 for TSH565 per 0.1 g of leaf tissue. Taq DNA Polymerase recombinant of Thermo Scientific® showed the highest performance specifically for this study, contributing to the undoubted amplification of molecular markers like microsatellites (SSRs). The results obtained have allowed improvements in genetic analyses and molecular studies using a reduced amount of plant tissue. |
| publishDate |
2018 |
| dc.date.issued.spa.fl_str_mv |
2018-04-01 |
| dc.date.accessioned.spa.fl_str_mv |
2019-07-03T05:51:10Z |
| dc.date.available.spa.fl_str_mv |
2019-07-03T05:51:10Z |
| dc.type.spa.fl_str_mv |
Artículo de revista |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_6501 |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Text |
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http://purl.org/redcol/resource_type/ART |
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publishedVersion |
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ISSN: 2323-0118 |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/68101 |
| dc.identifier.eprints.spa.fl_str_mv |
http://bdigital.unal.edu.co/69134/ |
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ISSN: 2323-0118 |
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https://repositorio.unal.edu.co/handle/unal/68101 http://bdigital.unal.edu.co/69134/ |
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spa |
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spa |
| dc.relation.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/63046 |
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Universidad Nacional de Colombia Revistas electrónicas UN Acta Agronómica Acta Agronómica |
| dc.relation.references.spa.fl_str_mv |
Ramírez, María Henao and Salazar Duque, Héctor Jaime and Urrea Trujillo, Aura Ines (2018) Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development. Acta Agronómica, 67 (2). pp. 311-318. ISSN 2323-0118 |
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Derechos reservados - Universidad Nacional de Colombia |
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http://purl.org/coar/access_right/c_abf2 |
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Atribución-NoComercial 4.0 Internacional |
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http://creativecommons.org/licenses/by-nc/4.0/ |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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application/pdf |
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Universidad Nacional de Colombia - Sede Palmira |
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Universidad Nacional de Colombia |
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Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ramírez, María Henao2ad581d8-cdd2-4fdf-95a6-a8a2038272eb300Salazar Duque, Héctor Jaime2aa26b89-b024-4fb5-a25f-977c31bb416c300Urrea Trujillo, Aura Ines744fd232-c848-48f2-ad9f-800c49da9c303002019-07-03T05:51:10Z2019-07-03T05:51:10Z2018-04-01ISSN: 2323-0118https://repositorio.unal.edu.co/handle/unal/68101http://bdigital.unal.edu.co/69134/Theobroma cacao L. and its products are consumed worldwide. Those products are of great research interest due to antioxidant properties of some of their polyphenolic constituents. The amount of these polyphenols and polysaccharides has shown that can interfere with the high quality and quantity of nucleic acids for molecular research. Therefore, cocoa DNA extraction protocols can require a large amount of plant material and optimization time according with plant material source. The aim of this study was to evaluate the quality and quantity of DNA isolated from field plant leaves at different developmental stages from TSH565 genotype using different DNA extraction protocols. In addition, DNA extraction protocol was evaluated for small amount of young leaf tissue collected from in vitro plantlets from CCN51 and TSH565 genotype. Subsequently, the selectivity of different polymerase enzymes for PCR amplification using the obtained DNA was evaluated. This study revealed that D stage of development in field leaves was efficient for extraction of high-quality genomic DNA using the PowerPlant® Pro Kit modified (183.80 ng.µL-1 (1.98 A260/A280-1.98 A260/A230)). Highest DNA concentrations were obtained for FPL with 128.68 ng.µL-1 and 114.42 ng.µL-1 for CCN51 and TSH565 respectively and with IVL, which was obtained 54.24 ng.µL-1 for CCN51 and 56.52 ng.µL-1 for TSH565 per 0.1 g of leaf tissue. Taq DNA Polymerase recombinant of Thermo Scientific® showed the highest performance specifically for this study, contributing to the undoubted amplification of molecular markers like microsatellites (SSRs). The results obtained have allowed improvements in genetic analyses and molecular studies using a reduced amount of plant tissue.Theobroma cacao L. y sus productos se consumen en todo el mundo. Esos productos son de gran interés para la investigación debido a las propiedades antioxidantes de algunos de sus componentes polifenólicos. La cantidad de estos polifenoles y polisacáridos ha demostrado que puede interferir con la alta calidad y cantidad de ácidos nucleicos para la investigación molecular. Por lo tanto, los protocolos de extracción de ADN de cacao pueden requerir una gran cantidad de material vegetal y tiempo de optimización de acuerdo con la fuente de origen del material vegetal. El objetivo de este estudio fue evaluar la calidad y la cantidad de ADN aislado de hojas de plantas de campo en diferentes etapas de desarrollo a partir del genotipo TSH565 utilizando diferentes protocolos de extracción de ADN. Además, se evaluó el protocolo de extracción de ADN para una pequeña cantidad de tejido foliar joven recogido de plántulas in vitro de genotipo CCN51 y TSH565. Posteriormente, se evaluó la selectividad de diferentes enzimas polimerasas para la amplificación por PCR usando el ADN obtenido. Este estudio reveló que la etapa D del desarrollo foliar en condiciones de campo fue eficiente para la extracción de ADN genómico de alta calidad usando el kit PowerPlant® Pro modificado (183.80 ng.μL-1 (1.98 A260 / A280-1.98 A260 / A230)). Las concentraciones más altas de ADN se obtuvieron para FPL con 128.68 ng.μL-1 y 114.42 ng.μL-1 para CCN51 y TSH565, respectivamente y con IVL, que se obtuvo 54.24 ng.μL-1 para CCN51 y 56.52 ng.μL-1 para TSH565 por 0.1 g de tejido foliar. Taq ADN polimerasa recombinante de Thermo Scientific® mostró el mayor rendimiento específicamente para este estudio, lo que contribuye a la amplificación indudable de marcadores moleculares como los microsatélites (SSR). Los resultados obtenidos han permitido mejoras en análisis genéticos y estudios moleculares utilizando una cantidad reducida de tejido vegetal.application/pdfspaUniversidad Nacional de Colombia - Sede Palmirahttps://revistas.unal.edu.co/index.php/acta_agronomica/article/view/63046Universidad Nacional de Colombia Revistas electrónicas UN Acta AgronómicaActa AgronómicaRamírez, María Henao and Salazar Duque, Héctor Jaime and Urrea Trujillo, Aura Ines (2018) Quality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of development. Acta Agronómica, 67 (2). pp. 311-318. ISSN 2323-011855 Ciencias de la tierra / Earth sciences and geology63 Agricultura y tecnologías relacionadas / AgricultureDNA isolationfield cocoa plantsin vitro cocoa plantletsleaf developmentPCRSSRsAislamiento de ADNplantas de cacao de campoplántulas de cacao in vitrodesarrollo de hojasPCRSSRsQuality of cocoa (Theobroma cacao L.) DNA from foliar tissue at different stages of developmentArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL63046-367757-4-PB.pdfapplication/pdf3528358https://repositorio.unal.edu.co/bitstream/unal/68101/1/63046-367757-4-PB.pdf14acd7b67d7a0eb34885ecac2d17b4d3MD51THUMBNAIL63046-367757-4-PB.pdf.jpg63046-367757-4-PB.pdf.jpgGenerated Thumbnailimage/jpeg9341https://repositorio.unal.edu.co/bitstream/unal/68101/2/63046-367757-4-PB.pdf.jpgcb0d26d8aa1eac774c8fa7793c835cb8MD52unal/68101oai:repositorio.unal.edu.co:unal/681012023-06-02 23:03:12.641Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co |