Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida

ilustraciones, diagramas, fotografías

Autores:: Rojas Hernández, Laura Camila

Tipo de recurso:

Fecha de publicación:: 2023

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida
dc.title.translated.eng.fl_str_mv	In vitro determination of the biocompatibility of a marker peptide coupled to USPIO nanoparticles in an induced neuroinflammation model.
title	Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida
spellingShingle	Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida 570 - Biología::572 - Bioquímica Enfermedades Neurodegenerativas Neurodegenerative Diseases Imágenes de resonancia magnética Nanopartículas de USPIO Biomarcador Magnetic resonance imaging USPIO nanoparticles
title_short	Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida
title_full	Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida
title_fullStr	Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida
title_full_unstemmed	Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida
title_sort	Determinación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducida
dc.creator.fl_str_mv	Rojas Hernández, Laura Camila
dc.contributor.advisor.none.fl_str_mv	Guerrero Fonseca, Carlos Arturo
dc.contributor.author.none.fl_str_mv	Rojas Hernández, Laura Camila
dc.contributor.researchgroup.spa.fl_str_mv	Biología celular y funcional e ingeniería de biomoléculas
dc.subject.ddc.spa.fl_str_mv	570 - Biología::572 - Bioquímica
topic	570 - Biología::572 - Bioquímica Enfermedades Neurodegenerativas Neurodegenerative Diseases Imágenes de resonancia magnética Nanopartículas de USPIO Biomarcador Magnetic resonance imaging USPIO nanoparticles
dc.subject.decs.spa.fl_str_mv	Enfermedades Neurodegenerativas
dc.subject.decs.eng.fl_str_mv	Neurodegenerative Diseases
dc.subject.proposal.spa.fl_str_mv	Imágenes de resonancia magnética Nanopartículas de USPIO Biomarcador
dc.subject.proposal.eng.fl_str_mv	Magnetic resonance imaging USPIO nanoparticles
description	ilustraciones, diagramas, fotografías
publishDate	2023
dc.date.issued.none.fl_str_mv	2023
dc.date.accessioned.none.fl_str_mv	2024-04-30T16:53:18Z
dc.date.available.none.fl_str_mv	2024-04-30T16:53:18Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/86003
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/86003 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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Functional diversity of astrocytes in neural circuit regulation. Nature Reviews Neuroscience 2016 18:1, 18(1), 31–41. https://doi.org/10.1038/NRN.2016.159 Biswas, S., Bachay, G., Chu, J., Hunter, D. D., & Brunken, W. J. (2017). Laminin-Dependent Interaction between Astrocytes and Microglia: A Role in Retinal Angiogenesis. The American Journal of Pathology, 187(9), 2112. https://doi.org/10.1016/J.AJPATH.2017.05.016 Chiu, A. Y., Espinosa De Los Monteros, A., Cole, R. A., Loera, S., & De Vellis, J. (1991). Laminin and slaminin are produced and released by astrocytes, schwann cells, and schwannomas in culture. Glia, 4(1), 11–24. https://doi.org/10.1002/GLIA.440040103
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Medicina - Maestría en Bioquímica
dc.publisher.faculty.spa.fl_str_mv	Facultad de Medicina
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Guerrero Fonseca, Carlos Arturocdd4ab53ceaeef988597f7e1a5dcf0b9Rojas Hernández, Laura Camilab79fd9ddd9b0e0cf39ce7a431812885aBiología celular y funcional e ingeniería de biomoléculas2024-04-30T16:53:18Z2024-04-30T16:53:18Z2023https://repositorio.unal.edu.co/handle/unal/86003Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografíasLos trastornos neurológicos son enfermedades degenerativas del sistema nervioso central (SNC) y constituyen una causa frecuente de morbilidad y mortalidad en el mundo, y han incrementado en los últimos años. Las nanopartículas USPIO (Ultrasmall Superparamagnetic Iron Oxide) son una categoría novedosa de agentes de contraste en resonancia magnética de imagen (RMI) que pueden acoplarse a diversas moléculas y dirigirse de manera específica hacia marcadores moleculares que reflejen alteraciones específicas. Por lo anterior, el objetivo del presente estudio fue caracterizar el efecto de las nanopartículas de USPIO conjugadas con el péptido marcador P88 en un modelo de neuroinflamación inducida in vitro con potencial aplicabilidad en RMI. Para lograr este objetivo, las líneas celulares HCMEC/D3 (células endoteliales de microvasculatura de cerebro) y T98G (astrocitos) se incubaron USPIO conjugadas con el péptido marcador y se determinó su efecto sobre la viabilidad celular por el método de LDH. Posteriormente, se identificó el perfil inflamatorio por medio de citometría de flujo y ensayos de PCR en tiempo real. Finalmente, se determinó el perfil de estrés oxidativo mediante la medición de los niveles de superóxido producidos. En general, los resultados de este estudio sugieren que NP88 es biocompatible con las líneas celulares HCMEC/D3 y T98G; y se podría utilizar potencialmente como biomarcador en modelos de neuroinflamación (Texto tomado de la fuente)Neurological disorders are degenerative diseases of the central nervous system (CNS) and constitute a frequent cause of morbidity and mortality in the world, and have increased in recent years. USPIO (Ultrasmall Superparamagnetic Iron Oxide) nanoparticles are a novel category of magnetic resonance imaging (MRI) contrast agents that can couple to various molecules and specifically target molecular markers that reflect specific abnormalities. Therefore, the objective of this study was to characterize the effect of USPIO nanoparticles conjugated with the marker peptide P88 in an in vitro induced neuroinflammation model with potential applicability in MRI. To achieve this goal, the cell lines HCMEC/D3 (brain microvasculature endothelial cells) and T98G (astrocytes) were incubated USPIO conjugated with the marker peptide and their effect on cell capacity was amplified by the LDH method. Subsequently, the inflammatory profile was identified by means of flow cytometry and real-time PCR assays. Finally, the oxidative stress profile was reduced by measuring the levels of superoxides produced. Overall, the results of this study suggest that NP88 is biocompatible with the HCMEC/D3 and T98G cell lines; and could potentially be used as a biomarker in models of neuroinflammationMaestríaMagíster en Bioquímica83 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina - Maestría en BioquímicaFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - BioquímicaEnfermedades NeurodegenerativasNeurodegenerative DiseasesImágenes de resonancia magnéticaNanopartículas de USPIOBiomarcadorMagnetic resonance imagingUSPIO nanoparticlesDeterminación in vitro de la biocompatibilidad de un péptido marcador acoplado a nanopartículas USPIO en un modelo de neuroinflamación inducidaIn vitro determination of the biocompatibility of a marker peptide coupled to USPIO nanoparticles in an induced neuroinflammation model.Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAfonina, I. S., Müller, C., Martin, S. J., & Beyaert, R. (2015). Proteolytic Processing of InterleukiAnnavarapu, S., & Nanda, V. (2009). Mirrors in the PDB: Left-handed -turns guide design with D-amino acids. BMC Structural Biology, 9. https://doi.org/10.1186/1472-6807-9-61Ben Haim, L., & Rowitch, D. H. (2016). Functional diversity of astrocytes in neural circuit regulation. Nature Reviews Neuroscience 2016 18:1, 18(1), 31–41. https://doi.org/10.1038/NRN.2016.159Biswas, S., Bachay, G., Chu, J., Hunter, D. D., & Brunken, W. J. (2017). Laminin-Dependent Interaction between Astrocytes and Microglia: A Role in Retinal Angiogenesis. The American Journal of Pathology, 187(9), 2112. https://doi.org/10.1016/J.AJPATH.2017.05.016Chiu, A. Y., Espinosa De Los Monteros, A., Cole, R. A., Loera, S., & De Vellis, J. (1991). Laminin and slaminin are produced and released by astrocytes, schwann cells, and schwannomas in culture. 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Functional diversity of astrocytes in neural circuit regulation. Nature Reviews Neuroscience 2016 18:1, 18(1), 31–41. https://doi.org/10.1038/NRN.2016.159Biswas, S., Bachay, G., Chu, J., Hunter, D. D., & Brunken, W. J. (2017). Laminin-Dependent Interaction between Astrocytes and Microglia: A Role in Retinal Angiogenesis. The American Journal of Pathology, 187(9), 2112. https://doi.org/10.1016/J.AJPATH.2017.05.016Chiu, A. Y., Espinosa De Los Monteros, A., Cole, R. A., Loera, S., & De Vellis, J. (1991). Laminin and slaminin are produced and released by astrocytes, schwann cells, and schwannomas in culture. Glia, 4(1), 11–24. https://doi.org/10.1002/GLIA.440040103InvestigadoresLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86003/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53ORIGINAL1019132780.2023.pdf1019132780.2023.pdfTesis de Maestría en Bioquímicaapplication/pdf1283339https://repositorio.unal.edu.co/bitstream/unal/86003/4/1019132780.2023.pdf650abc37991247b8e354bf38382efd5fMD54THUMBNAIL1019132780.2023.pdf.jpg1019132780.2023.pdf.jpgGenerated Thumbnailimage/jpeg5187https://repositorio.unal.edu.co/bitstream/unal/86003/5/1019132780.2023.pdf.jpg9d003ef16e150c61fbf7bb1a3a74df48MD55unal/86003oai:repositorio.unal.edu.co:unal/860032024-04-30 23:05:52.425Repositorio Institucional Universidad Nacional de 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