Estudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruzi

ilustraciones, diagramas, tablas

Autores:
Caldas Ortega, Luisa María
Tipo de recurso:
Fecha de publicación:
2024
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/86723
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/86723
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología::572 - Bioquímica
570 - Biología::571 - Fisiología y temas relacionados
INFECCIONES POR PROTOZOARIOS
ORGANISMOS UNICELULARES
NICOTINAMIDA
PROTEINA QUINASA C
Protozoan diseases
Unicelullar organisms
Nicotinamide
Niacin
Protein kinase C
Protozoan parasites
NADK
NAD(P)+/NAD(P)H
Parásitos protozoarios
NADK
NAD(P)+/NAD(P)H
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_60346262bc96d129a99336c442dbb4e2
oai_identifier_str oai:repositorio.unal.edu.co:unal/86723
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Estudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruzi
dc.title.translated.none.fl_str_mv Study of NADP+ metabolism in parasites of high incidence in public health: Exploring NAD kinase of Trypanosoma cruzi
title Estudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruzi
spellingShingle Estudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruzi
570 - Biología::572 - Bioquímica
570 - Biología::571 - Fisiología y temas relacionados
INFECCIONES POR PROTOZOARIOS
ORGANISMOS UNICELULARES
NICOTINAMIDA
PROTEINA QUINASA C
Protozoan diseases
Unicelullar organisms
Nicotinamide
Niacin
Protein kinase C
Protozoan parasites
NADK
NAD(P)+/NAD(P)H
Parásitos protozoarios
NADK
NAD(P)+/NAD(P)H
title_short Estudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruzi
title_full Estudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruzi
title_fullStr Estudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruzi
title_full_unstemmed Estudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruzi
title_sort Estudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruzi
dc.creator.fl_str_mv Caldas Ortega, Luisa María
dc.contributor.advisor.spa.fl_str_mv Ramírez Hernández, María Helena
dc.contributor.author.spa.fl_str_mv Caldas Ortega, Luisa María
dc.contributor.researchgroup.spa.fl_str_mv Libbiq Un
dc.subject.ddc.spa.fl_str_mv 570 - Biología::572 - Bioquímica
570 - Biología::571 - Fisiología y temas relacionados
topic 570 - Biología::572 - Bioquímica
570 - Biología::571 - Fisiología y temas relacionados
INFECCIONES POR PROTOZOARIOS
ORGANISMOS UNICELULARES
NICOTINAMIDA
PROTEINA QUINASA C
Protozoan diseases
Unicelullar organisms
Nicotinamide
Niacin
Protein kinase C
Protozoan parasites
NADK
NAD(P)+/NAD(P)H
Parásitos protozoarios
NADK
NAD(P)+/NAD(P)H
dc.subject.lemb.spa.fl_str_mv INFECCIONES POR PROTOZOARIOS
ORGANISMOS UNICELULARES
NICOTINAMIDA
PROTEINA QUINASA C
dc.subject.lemb.eng.fl_str_mv Protozoan diseases
Unicelullar organisms
Nicotinamide
Niacin
Protein kinase C
dc.subject.proposal.eng.fl_str_mv Protozoan parasites
NADK
NAD(P)+/NAD(P)H
dc.subject.proposal.spa.fl_str_mv Parásitos protozoarios
NADK
NAD(P)+/NAD(P)H
description ilustraciones, diagramas, tablas
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-08-13T15:45:24Z
dc.date.available.none.fl_str_mv 2024-08-13T15:45:24Z
dc.date.issued.none.fl_str_mv 2024
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/86723
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/86723
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Ramírez Hernández, María Helena3cd22161e24a39009214e3120ed6f39bCaldas Ortega, Luisa María6d22748533825e6137d50fa6f19a6731Libbiq Un2024-08-13T15:45:24Z2024-08-13T15:45:24Z2024https://repositorio.unal.edu.co/handle/unal/86723Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, tablasLos parásitos protozoarios son organismos eucariotas unicelulares causantes de gran variedad de enfermedades que afectan a cientos de millones de personas alrededor del mundo, constituyendo un desafío para la salud pública. Los tratamientos empleados actualmente presentan limitaciones a nivel de eficacia, seguridad y costo. En ese sentido, es necesario identificar nuevos blancos terapéuticos en las vías metabólicas esenciales para la supervivencia de los parásitos. El dinucleótido de adenina y nicotinamida (NAD+/NADH) y su forma fosforilada (NADP+/NADPH) son moléculas esenciales para la viabilidad y proliferación celular. La NAD quinasa es la única enzima capaz de generar NADP de novo y ha sido descrita y caracterizada en varias especies de eucariotas, arqueas y bacterias. En este trabajo se estudió la NAD quinasa de protozoarios de relevancia en la salud pública, mediante la generación de la proteína recombinante NAD quinasa de Trypanosoma cruzi (6xHis-SUMO-tTcNADK). Esta proteína permitió realizar los primeros ensayos para determinar la actividad enzimática, así como el desarrollo de una herramienta inmunológica esencial para establecer la localización citoplasmática de la proteína mediante técnicas de inmunodetección en epimastigotes. También fue posible analizar computacionalmente al candidato TcNADK en términos de predicción de sitios de escisión proteolítica, modificaciones covalentes e interacción proteína-proteína. Finalmente, en un análisis in silico se identificaron 18 secuencias candidatas a NADK en otras 16 especies de parásitos protozoarios, secuencias que codificarían para proteínas en su mayoría hidrofílicas con pesos moleculares que oscilan entre 21kDa y 195kDa, y que presentan los dominios y plegamientos característicos de las NAD quinasas, así como características únicas que serían relevantes para establecer blancos farmacológicos y entender la diversidad estructural de estas proteínas centrales en el metabolismo celular (Texto tomado de la fuente).Protozoan parasites are single-celled eukaryotic organisms that cause a variety of diseases affecting hundreds of millions of people around the world, posing a public health challenge. The treatments currently used are limited in terms of efficacy, safety and cost. In this sense, it is necessary to identify new therapeutic targets in the metabolic pathways essential for the survival of parasites. Adenine nicotinamide dinucleotide (NAD+/NADH) and its phosphorylated form (NADP+/NADPH) are essential molecules for cell viability and proliferation. NAD kinase is the only enzyme capable of generating de novo NADP and has been described and characterized in several species of eukaryotes, archaea and bacteria. In this work, the NAD kinase of protozoa of relevance in public health was studied, through the generation of the recombinant protein NAD kinase of Trypanosoma cruzi (6xHis-SUMO-tTcNADK). This protein allowed the first tests to figure out the enzyme activity, as well as the development of an essential immunological tool to establish the cytoplasmic localization of the protein by means of immunodetection techniques in epimastigotes. It was also possible to computationally analyze the TcNADK candidate in terms of prediction of proteolytic cleavage sites, covalent modifications and protein-protein interaction. Finally, an in silico analysis identified 18 NADK candidate sequences in 16 other protozoan parasite species, sequences that would code for mostly hydrophilic proteins with molecular weights ranging from 21kDa to 195kDa, and presenting the characteristic domains and folds of NAD kinases, as well as unique characteristics that would be relevant to establish pharmacological targets and understand the structural diversity of these central proteins in cell metabolism.Incluye ilustraciones, imágenes y tablasMaestríaMagíster en Ciencias - BioquímicaA partir de 100ng de ADN genómico de Trypanosoma cruzi cepa CL BRENER Esmerilado like se llevó a cabo la amplificación del gen completo (tcnadk) que codifica para el candidato a NAD quinasa, así como de una forma truncada correspondiente al dominio catalítico (ttcnadk) mediante PCR en tiempo final (Figura 5-1). El gen completo se amplificó empleando 1U de Phusion DNA polimerasa (Invitrogen) bajo la siguiente condición de reacción: 0.5μM primer directo y reverso, 0.2mM dNTPs, 1,25mM MgCl2, Buffer HF 1X, 0.2U de Phusion polimerasa; y el perfil térmico estandarizado. En el caso del dominio catalítico, la amplificación se ejecutó con la DNA polimerasa de Thermus aquaticus (Tabla 5-1) con 0.2 μM primer reverso y directo, 2 mM, dNTPs, 2mM MgCl2, Buffer de PCR 1X (20mM Tris-HCl pH 8.8, 10mM KCl, 0.1% (v/v) Triton X-100, 0.1 mg/ml de BSA) y agua DEPC. Los productos de PCR fueron visualizados mediante electroforesis de agarosa al 1% en buffer TBE (TrisBorato-EDTA).Metabolismo energético de parásitos protozoariosix, 73 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - Bioquímica570 - Biología::571 - Fisiología y temas relacionadosINFECCIONES POR PROTOZOARIOSORGANISMOS UNICELULARESNICOTINAMIDAPROTEINA QUINASA CProtozoan diseasesUnicelullar organismsNicotinamideNiacinProtein kinase CProtozoan parasitesNADKNAD(P)+/NAD(P)HParásitos protozoariosNADKNAD(P)+/NAD(P)HEstudio del metabolismo del NADP+ en parásitos de alta incidencia en la salud pública: Explorando la NAD Quinasa de Trypanosoma cruziStudy of NADP+ metabolism in parasites of high incidence in public health: Exploring NAD kinase of Trypanosoma cruziTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBeaumier, C.M., Gillespie, P.M., Hotez, P.J. & Bottazzi, M.E. 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PMID: 23297259; PMCID: PMC3553666.EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86723/3/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD53ORIGINAL1000514672.2024.pdf1000514672.2024.pdfTesis de Maestría en Bioquímicaapplication/pdf2653619https://repositorio.unal.edu.co/bitstream/unal/86723/4/1000514672.2024.pdf2c3735fb11c07d39301b991c74ad499aMD54THUMBNAIL1000514672.2024.pdf.jpg1000514672.2024.pdf.jpgGenerated Thumbnailimage/jpeg5619https://repositorio.unal.edu.co/bitstream/unal/86723/5/1000514672.2024.pdf.jpgc71bfe0b748f130b0cc88d9995ba503bMD55unal/86723oai:repositorio.unal.edu.co:unal/867232024-08-13 23:25:35.555Repositorio Institucional Universidad Nacional de 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