Obtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.

El objetivo principal de este trabajo fue evaluar el efecto del tamaño de poro en las propiedades mecánicas de andamios fabricados empleando Ɛ-policaprolactona mediante el uso de tecnología aditiva, con el fin de verificar un posible uso en ingeniería de tejidos, específicamente en tejido óseo. El d...

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Autores:
Flórez Prieto, Miguel Ángel
Tipo de recurso:
Fecha de publicación:
2023
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
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OAI Identifier:
oai:repositorio.unal.edu.co:unal/84793
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https://repositorio.unal.edu.co/handle/unal/84793
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología::572 - Bioquímica
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
670 - Manufactura::679 -Otros productos de materiales específicos
Imagen tridimensional en diseño
Sistemas de representacion tridimencional
Design imaging
Three-dimensional display systems
Tecnología aditiva
Andamios
Pruebas mecánicas
Análisis de imagen
Additive technology
Scaffolds
Mechanical tests
Image analysis
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openAccess
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Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_5fec24fdf0c8131bba12427d3bb03a6e
oai_identifier_str oai:repositorio.unal.edu.co:unal/84793
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Obtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.
dc.title.translated.eng.fl_str_mv Obtaining a scaffold with potential use in tissue engineering using polycaprolactone in a 3D printer.
title Obtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.
spellingShingle Obtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.
570 - Biología::572 - Bioquímica
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
670 - Manufactura::679 -Otros productos de materiales específicos
Imagen tridimensional en diseño
Sistemas de representacion tridimencional
Design imaging
Three-dimensional display systems
Tecnología aditiva
Andamios
Pruebas mecánicas
Análisis de imagen
Additive technology
Scaffolds
Mechanical tests
Image analysis
title_short Obtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.
title_full Obtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.
title_fullStr Obtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.
title_full_unstemmed Obtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.
title_sort Obtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.
dc.creator.fl_str_mv Flórez Prieto, Miguel Ángel
dc.contributor.advisor.none.fl_str_mv Perilla Perilla, Jairo Ernesto
Godoy Silva, Rubén Darío
dc.contributor.author.none.fl_str_mv Flórez Prieto, Miguel Ángel
dc.contributor.researchgroup.spa.fl_str_mv Grupo de Investigación en Procesos Químicos y Bioquímicos
dc.contributor.cvlac.spa.fl_str_mv FLÓREZ PRIETO, MIGUEL ÁNGEL
dc.subject.ddc.spa.fl_str_mv 570 - Biología::572 - Bioquímica
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
670 - Manufactura::679 -Otros productos de materiales específicos
topic 570 - Biología::572 - Bioquímica
540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales
670 - Manufactura::679 -Otros productos de materiales específicos
Imagen tridimensional en diseño
Sistemas de representacion tridimencional
Design imaging
Three-dimensional display systems
Tecnología aditiva
Andamios
Pruebas mecánicas
Análisis de imagen
Additive technology
Scaffolds
Mechanical tests
Image analysis
dc.subject.lemb.spa.fl_str_mv Imagen tridimensional en diseño
Sistemas de representacion tridimencional
dc.subject.lemb.eng.fl_str_mv Design imaging
Three-dimensional display systems
dc.subject.proposal.spa.fl_str_mv Tecnología aditiva
Andamios
Pruebas mecánicas
Análisis de imagen
dc.subject.proposal.eng.fl_str_mv Additive technology
Scaffolds
Mechanical tests
dc.subject.proposal.none.fl_str_mv Image analysis
description El objetivo principal de este trabajo fue evaluar el efecto del tamaño de poro en las propiedades mecánicas de andamios fabricados empleando Ɛ-policaprolactona mediante el uso de tecnología aditiva, con el fin de verificar un posible uso en ingeniería de tejidos, específicamente en tejido óseo. El diseño metodológico involucró tres fases: la primera consistió en el desarrollo del diseño del andamio a utilizar, además de evaluar las condiciones de impresión que pueden afectar la resolución y forma de los andamios, para finalmente imprimir las muestras a evaluar. La segunda fase consistió en realizar pruebas de caracterización del polímero, como calorimetría diferencial de barrido y análisis termogravimétrico. Además, se llevaron a cabo pruebas mecánicas de tensión-deformación, compresión y flexión. Finalmente, en la tercera etapa se evaluó la biocompatibilidad del polímero para determinar si en un futuro puede ser aplicable para la regeneración ósea. Como resultado, luego de evaluar el efecto de la temperatura en la construcción de andamios de 500 µm, 750 µm y 1000 µm, se logró detectar que los cambios son poco significativos en las propiedades mecánicas al variar las condiciones de impresión en temperaturas de 175°C, 185°C, 195°C y 205°C, y en el lecho de: 50°C, 55°C, 60°C, 65°C y 70°C. Sin embargo, al variar el espaciamiento del filamento, las propiedades mecánicas de tensión y flexión aumentaron conforme disminuyó el espaciamiento. En el caso de las propiedades de compresión, los resultados no mostraron una variación considerable. La toxicidad del polímero es baja, lo que permite su uso en ingeniería de tejidos. (Texto tomado de la fuente)
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-10-10T21:55:57Z
dc.date.available.none.fl_str_mv 2023-10-10T21:55:57Z
dc.date.issued.none.fl_str_mv 2023-08-10
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/84793
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/84793
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
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dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
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spelling Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Perilla Perilla, Jairo Ernesto6da974f5d7b47336a5ddf3bb9482b009600Godoy Silva, Rubén Darío019810b6b9f5c2a275ca1c832cf9cda7600Flórez Prieto, Miguel Ángel135029418c0d4afc08d005cedfc0e332Grupo de Investigación en Procesos Químicos y BioquímicosFLÓREZ PRIETO, MIGUEL ÁNGEL2023-10-10T21:55:57Z2023-10-10T21:55:57Z2023-08-10https://repositorio.unal.edu.co/handle/unal/84793Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/El objetivo principal de este trabajo fue evaluar el efecto del tamaño de poro en las propiedades mecánicas de andamios fabricados empleando Ɛ-policaprolactona mediante el uso de tecnología aditiva, con el fin de verificar un posible uso en ingeniería de tejidos, específicamente en tejido óseo. El diseño metodológico involucró tres fases: la primera consistió en el desarrollo del diseño del andamio a utilizar, además de evaluar las condiciones de impresión que pueden afectar la resolución y forma de los andamios, para finalmente imprimir las muestras a evaluar. La segunda fase consistió en realizar pruebas de caracterización del polímero, como calorimetría diferencial de barrido y análisis termogravimétrico. Además, se llevaron a cabo pruebas mecánicas de tensión-deformación, compresión y flexión. Finalmente, en la tercera etapa se evaluó la biocompatibilidad del polímero para determinar si en un futuro puede ser aplicable para la regeneración ósea. Como resultado, luego de evaluar el efecto de la temperatura en la construcción de andamios de 500 µm, 750 µm y 1000 µm, se logró detectar que los cambios son poco significativos en las propiedades mecánicas al variar las condiciones de impresión en temperaturas de 175°C, 185°C, 195°C y 205°C, y en el lecho de: 50°C, 55°C, 60°C, 65°C y 70°C. Sin embargo, al variar el espaciamiento del filamento, las propiedades mecánicas de tensión y flexión aumentaron conforme disminuyó el espaciamiento. En el caso de las propiedades de compresión, los resultados no mostraron una variación considerable. La toxicidad del polímero es baja, lo que permite su uso en ingeniería de tejidos. (Texto tomado de la fuente)ilustraciones, diagramas, fotografíasThe overarching objective of this study was to assess the impact of pore size on the mechanical attributes of scaffolds produced using Ɛ-polycaprolactone via additive manufacturing, with the aim of validating its potential applicability in tissue engineering, particularly for bone tissue. The methodological design encompassed three phases: the first phase involved developing the scaffold design for deployment, along with evaluating the print conditions that could influence scaffold resolution and form, culminating in the printing of the samples for evaluation. The second phase entailed executing polymer characterization tests, including differential scanning calorimetry and thermogravimetric analysis. In addition to these, mechanical stress-strain, compression, and bending tests were performed. Lastly, during the third stage, the polymer's biocompatibility was examined to ascertain its prospective utility in future bone regeneration efforts. The outcomes indicated that, upon assessing the temperature's influence on the construction of scaffolds with pore sizes of 500 µm, 750 µm, and 1000 µm, the observed changes in mechanical properties exhibited minimal significance when the print conditions were altered at temperatures of 175°C, 185°C, 195°C, and 205°C, as well as the bed temperature variations of 50°C, 55°C, 60°C, 65°C, and 70°C. However, in instances of modifying filament spacing, mechanical tensile and bending properties were found to escalate inversely with spacing reduction. As for compression properties, the results displayed nominal variance. The polymer's toxicity was found to be low, thus facilitating its utilization in tissue engineering applications.MaestríaMagíster en Ingeniería - Ingeniería QuímicaLa investigación se basa en un desarrollo experimental que se da en diferentes etapas, la primera fue el diseño del andamio para poder verificar las condiciones de impresión optimas que permitieran una resolución adecuada, segundo un analisis de variacion de condiciones de impresión para determinar el efecto en la resolución, todo ello apoyado con programas de analisis de imagen, tercero, elaboración de pruebas mecánicas: tensión, compresión y flexión a los andamios. Cuarto modificación de tamaño de poro con el fin de evaluar las propiedades mecánicas y determinar su efecto. y Finalmente de evaluó la citotoxicidad del material.Ingeniería de tejidos.Bioprocesosxxi, 168 páginasapplication/pdfUniversidad Nacional de ColombiaBogotá - Ingeniería - Maestría en Ingeniería - Ingeniería QuímicaFacultad de IngenieríaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - Bioquímica540 - Química y ciencias afines::542 - Técnicas, procedimientos, aparatos, equipos, materiales670 - Manufactura::679 -Otros productos de materiales específicosImagen tridimensional en diseñoSistemas de representacion tridimencionalDesign imagingThree-dimensional display systemsTecnología aditivaAndamiosPruebas mecánicasAnálisis de imagenAdditive technologyScaffoldsMechanical testsImage analysisObtención de un andamio con potencial uso en ingeniería de tejidos empleando policaprolactona en una impresora 3D.Obtaining a scaffold with potential use in tissue engineering using polycaprolactone in a 3D printer.Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbdelhamid, M. 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ACS Biomaterials Science & Engineering, 9(8), 4583–4596. https://doi.org/10.1021/acsbiomaterials.3c00564EstudiantesLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84793/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1020787186.2023.pdf1020787186.2023.pdfTesis de Maestría en Ingeniería - Ingeniería Químicaapplication/pdf4321315https://repositorio.unal.edu.co/bitstream/unal/84793/2/1020787186.2023.pdf8ef2917f5047f582d9b82b6f457650abMD52THUMBNAIL1020787186.2023.pdf.jpg1020787186.2023.pdf.jpgGenerated Thumbnailimage/jpeg4872https://repositorio.unal.edu.co/bitstream/unal/84793/3/1020787186.2023.pdf.jpg6915399d74c3f8a00407d35c30cb7decMD53unal/84793oai:repositorio.unal.edu.co:unal/847932024-08-19 23:10:23.921Repositorio Institucional Universidad Nacional de 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