Efecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivo
diagramas, ilustraciones a color, fotografías, tablas
- Autores:
-
González Mendoza, Daniel Fernando
- Tipo de recurso:
- Fecha de publicación:
- 2020
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/79502
- Palabra clave:
- 630 - Agricultura y tecnologías relacionadas::636 - Producción animal
Criopreservación
Cryopreservation
Vitrificación
Vitrification
Ovinos
Sheep
Criopreservación
Ovejas
Vitrificación
Pajilla Abierta (OPS)
Pajilla 0,25.
Cryopreservation
Sheep
Ultra-Fast Freezing
Open Straw (OPS)
Straw 0,25 cc
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Efecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivo |
| dc.title.translated.eng.fl_str_mv |
Effect of vitrification protocol and packaging system on the survival rate of in vivo derived ovine embryos |
| title |
Efecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivo |
| spellingShingle |
Efecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivo 630 - Agricultura y tecnologías relacionadas::636 - Producción animal Criopreservación Cryopreservation Vitrificación Vitrification Ovinos Sheep Criopreservación Ovejas Vitrificación Pajilla Abierta (OPS) Pajilla 0,25. Cryopreservation Sheep Ultra-Fast Freezing Open Straw (OPS) Straw 0,25 cc |
| title_short |
Efecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivo |
| title_full |
Efecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivo |
| title_fullStr |
Efecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivo |
| title_full_unstemmed |
Efecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivo |
| title_sort |
Efecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivo |
| dc.creator.fl_str_mv |
González Mendoza, Daniel Fernando |
| dc.contributor.advisor.none.fl_str_mv |
Jiménez Escobar, Claudia Zambrano, Jorge |
| dc.contributor.author.none.fl_str_mv |
González Mendoza, Daniel Fernando |
| dc.contributor.researchgroup.spa.fl_str_mv |
Reproducción Animal y Salud de Hato |
| dc.subject.ddc.spa.fl_str_mv |
630 - Agricultura y tecnologías relacionadas::636 - Producción animal |
| topic |
630 - Agricultura y tecnologías relacionadas::636 - Producción animal Criopreservación Cryopreservation Vitrificación Vitrification Ovinos Sheep Criopreservación Ovejas Vitrificación Pajilla Abierta (OPS) Pajilla 0,25. Cryopreservation Sheep Ultra-Fast Freezing Open Straw (OPS) Straw 0,25 cc |
| dc.subject.agrovoc.none.fl_str_mv |
Criopreservación Cryopreservation Vitrificación Vitrification Ovinos Sheep |
| dc.subject.proposal.spa.fl_str_mv |
Criopreservación Ovejas Vitrificación Pajilla Abierta (OPS) Pajilla 0,25. |
| dc.subject.proposal.eng.fl_str_mv |
Cryopreservation Sheep Ultra-Fast Freezing Open Straw (OPS) Straw 0,25 cc |
| description |
diagramas, ilustraciones a color, fotografías, tablas |
| publishDate |
2020 |
| dc.date.issued.none.fl_str_mv |
2020-11-13 |
| dc.date.accessioned.none.fl_str_mv |
2021-05-11T20:10:30Z |
| dc.date.available.none.fl_str_mv |
2021-05-11T20:10:30Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/79502 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/79502 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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S. S., & Aschenbrenner, G. A. (2020). EFFECT OF FLUNIXIN MEGLUMINE AND hCG AT COMMERCIAL PROGRAMS FOR MULTIPLE OVULATION AND EMBRYO TRANSFER (MOET) IN SHEEP. Archives of Veterinary Science, 5(1), 56–66. www.ser.ufpr.br/veterinary Bergstein-Galan, Tácia Gomes, Weiss, R. R., & Kozicki, L. E. (2019). Effect of semen and donor factors on multiple ovulation and embryo transfer (MOET) in sheep. Reproduction in Domestic Animals, 54(2), 401–407. https://doi.org/10.1111/rda.13381 Bettencourt, E. M., Bettencourt, C. M., Silva, J. C. e., Ferreira, P., Manito, C. I., Matos, C. M., Romão, R. J., & Rocha, A. (2008). Effect of season and gonadotrophin preparation on superovulatory response and embryo quality in Portuguese Black Merinos. Small Ruminant Research, 74(1–3), 134–139. https://doi.org/10.1016/j.smallrumres.2007.05.001 Bettencourt, E. M. V., Bettencourt, C. M., Silva, J. N. C. E., Ferreira, P., de Matos, C. P., Oliveira, E., Romão, R. J., Rocha, A., & Sousa, M. (2009). Ultrastructural characterization of fresh and cryopreserved in vivo produced ovine embryos. Theriogenology, 71(6), 947–958. https://doi.org/10.1016/j.theriogenology.2008.10.019 Bhat, M. H., Sharma, V., Khan, F. A., Naykoo, N. A., Yaqoob, S. H., Vajta, G., Khan, H. M., Fazili, M. R., Ganai, N. A., & Shah, R. A. (2015). Open pulled straw vitrification and slow freezing of sheep IVF embryos using different cryoprotectants. Reproduction, Fertility and Development, 27(8), 1175–1180. https://doi.org/10.1071/RD14024 Bielanski, A., & Vajta, G. (2009). Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units. In Human Reproduction (Vol. 24, Issue 10, pp. 2457–2467). Oxford University Press. https://doi.org/10.1093/humrep/dep117 Blanco, M. R., Simonetti, L., & Rivera, O. E. (2003). Embryo production and progesterone profiles in ewes superovulated with different hormonal treatments. Small Ruminant Research, 47(3), 183–191. https://doi.org/10.1016/S0921-4488(02)00245-6 Bottrel, M., Hidalgo, M., Mogas, T., Pereira, B., Ortiz, I., Díaz-Jiménez, M., Consuegra, C., Morató, R., & Dorado, J. (2020). One-step warming does not affect the in vitro viability and cryosurvival of cryotop-vitrified donkey embryos. Theriogenology, 152, 47–52. https://doi.org/10.1016/j.theriogenology.2020.04.026 Brasil, O. O., Moreira, N. H., Santos, G., Silva, B. D. M., Mariante, A. S., & Ramos, A. F. (2016). Superovulatory and embryo yielding in sheep using increased exposure time to progesterone associated with a GnRH agonist. Small Ruminant Research, 136, 54–58. https://doi.org/10.1016/j.smallrumres.2016.01.005 Bruno-Galarraga, M., Cueto, M., Gibbons, A., Pereyra-Bonnet, F., Subiabre, M., & González-Bulnes, A. (2015). Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs. Theriogenology, 84(5), 784–790. https://doi.org/10.1016/j.theriogenology.2015.05.011 Caamaño, J. N., Gómez, E., Trigal, B., Muñoz, M., Carrocera, S., Martín, D., & Díez, C. (2015). Survival of vitrified invitro-produced bovine embryos after a one-step warming in-straw cryoprotectant dilution procedure. Theriogenology, 83(5), 881–890. https://doi.org/10.1016/j.theriogenology.2014.11.021 Chian, R. C., Son, W. Y., Huang, J. Y., Cui, S. J., Buckett, W. M., & Tan, S. L. (2005). High survival rates and pregnancies of human oocytes following vitrification: preliminary report. Fertility and Sterility, 84, S26, 2005. https://doi.org/https://doi.org/10.1016/j.fertnstert.2005.07.086 Cocero, M., Aguilar., B., Alabart, J. L., Olivera, J., & Folch, J. (2000). FACTORES QUE AFECTAN AL RENDIMIENTO DE LA T.E.CONGELADOS EN EL PROGRAMA GENÉTICO DE OVIARAGON. 760–762. Cognie, Y. (1999). State of the art in sheep-goat embryo transfer. Theriogenology, 51(1), 105–116. https://doi.org/10.1016/S0093-691X(98)00235-0 Contreras-Solis, I., Diaz, T., Lopez, G., Caigua, A., Lopez-Sebastian, A., & Gonzalez-Bulnes, A. (2008). Systemic and intraovarian effects of corpus luteum on follicular dynamics during estrous cycle in hair breed sheep. Animal Reproduction Science, 104(1), 47–55. https://doi.org/10.1016/j.anireprosci.2007.01.021 Cortés-Reyes, É., Rubio-Romero, J. A., & Gaitán-Duarte, H. (2010). Statistical methods for evaluating diagnostic test agreement and reproducibility. Revista Colombiana de Obstetricia y Ginecologia, 61(3), 247–255. https://doi.org/10.18597/rcog.271 Crilly, J. P., Politis, A. P., & Hamer, K. (2017). Use of ultrasonographic examination in sheep veterinary practice. Small Ruminant Research, 152(July), 166–173. https://doi.org/10.1016/j.smallrumres.2016.12.021 Cuadro, F., dos Santos-Neto, P. C., Pinczak, A., Barrera, N., Crispo, M., & Menchaca, A. (2018). 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Journal of Animal Science and Biotechnology, 5(1), 1–7. https://doi.org/10.1186/2049-1891-5-19 |
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info:eu-repo/semantics/openAccess |
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Atribución-NoComercial-SinDerivadas 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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1 recurso en línea (108 páginas) |
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application/pdf |
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Universidad Nacional de Colombia |
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Bogotá - Medicina Veterinaria y de Zootecnia - Maestría en Salud y Producción Animal |
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Facultad de Medicina Veterinaria y de Zootecnia |
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Bogotá |
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Universidad Nacional de Colombia - Sede Bogotá |
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Universidad Nacional de Colombia |
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https://repositorio.unal.edu.co/bitstream/unal/79502/4/2949613899.2020.pdf https://repositorio.unal.edu.co/bitstream/unal/79502/2/license.txt https://repositorio.unal.edu.co/bitstream/unal/79502/3/license_rdf https://repositorio.unal.edu.co/bitstream/unal/79502/5/2949613899.2020.pdf.jpg |
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Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Jiménez Escobar, Claudiaf813567a35b606404b1676a988af6d79Zambrano, Jorge179e9da4e31ffe8da3fbc2ce59023ce9González Mendoza, Daniel Fernandoad82b8eb0b32a0bf6f4f283226bb7f72Reproducción Animal y Salud de Hato2021-05-11T20:10:30Z2021-05-11T20:10:30Z2020-11-13https://repositorio.unal.edu.co/handle/unal/79502Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/diagramas, ilustraciones a color, fotografías, tablasLa presente investigación tuvo como fin evaluar metodologías que faciliaran el proceso de vitrificación de embriones ovinos y la eliminación de potenciales contaminantes, comparando dos sistemas de empaque (abierto y cerrado) ya que el uso de empaques abiertos se consideran un riesgo de bioseguridad en este tipo de biotecnología reproductiva. Para este fin, se seleccionaron 25 ovejas trihibridas adultas en buenas condiciones sanitarias y nutricionales, cruces de razas Katahdin, Pelibuey y Dorset entre el primero y sexto parto. Se sincronizaron un grupo de cinco ovejas, cada 7 días durante cinco semanas mediante esponjas vaginales impregnadas con 60mg de Acetato Medroxiprogesterona por 13 días; además, se realizó un tratamiento de superovulación entre el día 11 al 14 de tratamiento con 160mg de Hormona Folículo Estimulante porcina (pFSH) en ocho dosis decrecientes, cada 12h paralelamente con la remoción del dispositivo vaginal en el día 13. Por otro lado, se realizó detección de celo con un macho vasectomizado y monta natural controlada, al día siete de esta se realizó recolección de embriones por medio de laparotomía. Subsiguientemente se evaluaron y clasificaron los embriones de acuerdo con las pautas de la Sociedad Internacional de Transferencia de Embriones, y se procedió a realizar criopreservación de sólo embriones categorizados como buenos y excelentes. Se obtuvieron 222 embriones de los cuales se criopreservaron 220, tomando un primer grupo (n=110, 50%) que se vitrificó mediante empaque Abierto (open-pulled Straw, OPS) y el segundo tratamiento experimental (n=110, 50%) se vitrificó a través del método cerrado con pajillas de 0.25cc. Ambos tratamientos divididos equitativamente por etapa de desarrollo y tipo de empaque. Se desvitrificaron 160 embriones, de los 220 congelados, ochenta por cada método de vitrificación los cuales fueron cultivados en una incubadora con 5% de CO2 en aire a 39° C por 72 horas, determinando el porcentaje de embriones reexpandidos a 24, 48 y 72 horas post desvitrificación y su tasa de eclosión, obteniendo una viabilidad del 72,6% (n= 80) para los embriones vitrificados por OPS y del 67,6% (n=80) para los embriones vitrificados por pajilla. Posteriormente se compararon las tasas de supervivencia embrionaria con la prueba de chi-cuadrado con un p-valor 0,49, concluyendo que no existe dependencia o asociación entre la variable (supervivencia) y el factor (empaque); las diferencias significativas se tomaron con un p<0,05. Finalmente, con este estudio se logró aplicar con éxito biotecnologías reproductivas como la ultrasonografía, laparoscopia, sincronización, superovulación, transferencia de embriones y criopreservación de embriones por medio de la vitrificación con dos sistemas de empaque en ovejas trihibridas bajo nuestras condiciones ambientales y de manejo. Considerando que el empaque cerrado es mejor en términos de bioseguridad y que no hubo diferencias con el sistema OPS, se recomienda trabajar el sistema cerrado para vitrificar embriones ovinos.The purpose of this research was to evaluate two different packaging systems to cryopreserve ovine embryos avoiding potential contaminants in vitrification processes related to the use of open packages such as the open-pulled straw system, which is considered to be unsuitable for use in commercial embryo cryopreservation. For this purpose, 25 adult trihybrid ewes (crosses of Katahdin, Pelibuey and Dorset) in good sanitary and nutritional conditions were selected between the first and sixth lambing. Groups of five ewes were synchronized every 7 days for five weeks with intravaginal pessaries impregnated with 60mg of Medroxyprogesterone Acetate for 13 days. In addition, a superovulation treatment was performed between the 11th and 14th day of treatment with 160mg of porcine Follicle-Stimulating Hormone (pFSH) in eight decreasing doses every 12h. Controlled natural breeding was performed after estrus detection with a vasectomized male; seven days after natural breeding, embryo collection was performed by means of laparotomy. Subsequently, the embryos were evaluated and classified according to the guidelines of the International Embryo Transfer Society and only embryos categorized as excellent and good were used in cryopreservation treatments. From the 222 embryos obtained, 220 were cryopreserved; embryos were divided in two treatment groups: first group (n=110, 50%) which were vitrified in open-pulled straws (OPS) and the second group (n=110, 50%) were vitrified in 0.25cc straws. Both treatments were equally divided by developmental stage and type of packaging. For survival evaluation, 160 of the 220 embryos were devitrified, eighty by each method of packaging. These were immediately cultured in an incubator with 5% CO2 in air at 39° C for 72 hours, determining a percentage of embryos re-expanded at 24, 48 and 72 hours post-devitrification and the hatching rate. A viability of 72.6% (n=80) for the embryos vitrified by OPS and 67.6% (n=80) for the embryos vitrified by straw were obtained. Embryo survival rates were not significantly different among the two packaging methods (chi-square test) with a pvalue of 0.49. Finally, with this study, reproductive biotechnologies such as ultrasonography, laparoscopy, synchronization, superovulation, embryo transfer, and cryopreservation of embryos through vitrification with two packing systems were successfully applied in trihybrid ewes. Considering that a closed packaging system is safer in terms of biosecurity and that there were no significant differences in survival rates compared to the OPS system, we recommend to freeze sheep embryos with closed techniques such as the use of 0.25 cc straws.Maestría1 recurso en línea (108 páginas)application/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina Veterinaria y de Zootecnia - Maestría en Salud y Producción AnimalFacultad de Medicina Veterinaria y de ZootecniaBogotáUniversidad Nacional de Colombia - Sede Bogotá630 - Agricultura y tecnologías relacionadas::636 - Producción animalCriopreservaciónCryopreservationVitrificaciónVitrificationOvinosSheepCriopreservaciónOvejasVitrificaciónPajilla Abierta (OPS)Pajilla 0,25.CryopreservationSheepUltra-Fast FreezingOpen Straw (OPS)Straw 0,25 ccEfecto del protocolo de vitrificación y sistemas de empaque sobre la tasa de supervivencia de embriones ovinos obtenidos in vivoEffect of vitrification protocol and packaging system on the survival rate of in vivo derived ovine embryosTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbecia, J. A., Forcada, F., Palacín, I., Sánchez-Prieto, L., Sosa, C., Fernández-Foren, A., & Meikle, A. (2015). Undernutrition affects embryo quality of superovulated ewes. Zygote, 23(1), 116–124. https://doi.org/10.1017/S096719941300035XAbubakar, A. A., Andeshi, R. A., Yakubu, A. S., Lawal, F. M., & Adamu, U. (2014). Comparative Evaluation of Midventral and Flank Laparotomy Approaches in Goat. Journal of Veterinary Medicine, 2014, 1–6. https://doi.org/10.1155/2014/920191Aké-lópez, J. R., Centurión-castro, F. G., Magaña-monforte, J. G., & Aké-villanueva, J. R. (2014). Efecto del progestágeno y de la dosis de gonadotropina corionica equina en la sincronización del estro y tasa de gestación en ovejas Pelibuey inseminadas por laparoscopia. Ecosistemas y Recursos Agropecuarios, 1(3), 261–268.Akiyama, K., Kobayashi, J., Sato, Y., Sata, R., Ohashi, M., Sasaki, E., Oda, Y., Ogawa, Y., Ueda, S., Nabenishi, H., & matoba, S. (2010). Calf production from vitrified bovine sexed embryos following in-straw dilution. Animal Science Journal, 81(4), 461–466. https://doi.org/10.1111/j.1740-0929.2010.00763.xAlmodin, C. G., Minguetti-Camara, V. C., Paixao, C. L., & Pereira, P. C. (2010). Embryo development and gestation using fresh and vitrified oocytes. Human Reproduction, 25(5), 1192–1198. https://doi.org/10.1093/humrep/deq042Arévalo Garay, Á., & Correa Assmus, G. (2013). Tecnología en la ovinocultura colombiana: estado del arte. Ciencia Animal, 6, 125–142.Asgari, V., Hosseini, S. M., Ostadhosseini, S., Hajian, M., Azhdari, Z. T., Mosaie, M., & Nasr-Esfahani, M. H. (2012). Specific activation requirements of in vitro-matured sheep oocytes following vitrification-warming. Molecular Reproduction and Development, 79(7), 434–444. https://doi.org/10.1002/mrd.22047Baril, G., Traldi, a. L., Cognié, Y., Leboeuf, B., Beckers, J. F., & Mermillod, P. (2001). Successful direct transfer of vitrified sheep embryos. Theriogenology, 56(2), 299–305. https://doi.org/10.1016/S0093-691X(01)00564-7Bartlewski, P. M., Seaton, P., Franco Oliveira, M. E., Kridli, R. T., Murawski, M., & Schwarz, T. (2016). Intrinsic determinants and predictors of superovulatory yields in sheep: Circulating concentrations of reproductive hormones, ovarian status, and antral follicular blood flow. Theriogenology, 86(1), 130–143. https://doi.org/10.1016/j.theriogenology.2016.04.024Bartlewski, P. M., Seaton, P., Szpila, P., Oliveira, M. E. F., Murawski, M., Schwarz, T., Kridli, R. T., & Zieba, D. A. (2015). Comparison of the effects of pretreatment with Veramix sponge (medroxyprogesterone acetate) or CIDR (natural progesterone) in combination with an injection of estradiol-17β on ovarian activity, endocrine profiles, and embryo yields in cyclic ewes superovu. Theriogenology, 84(7), 1225–1237. https://doi.org/10.1016/j.theriogenology.2015.07.002Bergstein-Galan, T. G., Weiss, R. R., Kozicki, L. E., Bortoleto, C. T., Lara, N. S. S., & Aschenbrenner, G. A. (2020). EFFECT OF FLUNIXIN MEGLUMINE AND hCG AT COMMERCIAL PROGRAMS FOR MULTIPLE OVULATION AND EMBRYO TRANSFER (MOET) IN SHEEP. Archives of Veterinary Science, 5(1), 56–66. www.ser.ufpr.br/veterinaryBergstein-Galan, Tácia Gomes, Weiss, R. R., & Kozicki, L. E. (2019). Effect of semen and donor factors on multiple ovulation and embryo transfer (MOET) in sheep. Reproduction in Domestic Animals, 54(2), 401–407. https://doi.org/10.1111/rda.13381Bettencourt, E. M., Bettencourt, C. M., Silva, J. C. e., Ferreira, P., Manito, C. I., Matos, C. M., Romão, R. J., & Rocha, A. (2008). Effect of season and gonadotrophin preparation on superovulatory response and embryo quality in Portuguese Black Merinos. Small Ruminant Research, 74(1–3), 134–139. https://doi.org/10.1016/j.smallrumres.2007.05.001Bettencourt, E. M. V., Bettencourt, C. M., Silva, J. N. C. E., Ferreira, P., de Matos, C. P., Oliveira, E., Romão, R. J., Rocha, A., & Sousa, M. (2009). Ultrastructural characterization of fresh and cryopreserved in vivo produced ovine embryos. Theriogenology, 71(6), 947–958. https://doi.org/10.1016/j.theriogenology.2008.10.019Bhat, M. H., Sharma, V., Khan, F. A., Naykoo, N. A., Yaqoob, S. H., Vajta, G., Khan, H. M., Fazili, M. R., Ganai, N. A., & Shah, R. A. (2015). Open pulled straw vitrification and slow freezing of sheep IVF embryos using different cryoprotectants. Reproduction, Fertility and Development, 27(8), 1175–1180. https://doi.org/10.1071/RD14024Bielanski, A., & Vajta, G. (2009). Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units. In Human Reproduction (Vol. 24, Issue 10, pp. 2457–2467). Oxford University Press. https://doi.org/10.1093/humrep/dep117Blanco, M. R., Simonetti, L., & Rivera, O. E. (2003). Embryo production and progesterone profiles in ewes superovulated with different hormonal treatments. Small Ruminant Research, 47(3), 183–191. https://doi.org/10.1016/S0921-4488(02)00245-6Bottrel, M., Hidalgo, M., Mogas, T., Pereira, B., Ortiz, I., Díaz-Jiménez, M., Consuegra, C., Morató, R., & Dorado, J. (2020). One-step warming does not affect the in vitro viability and cryosurvival of cryotop-vitrified donkey embryos. Theriogenology, 152, 47–52. https://doi.org/10.1016/j.theriogenology.2020.04.026Brasil, O. O., Moreira, N. H., Santos, G., Silva, B. D. M., Mariante, A. S., & Ramos, A. F. (2016). Superovulatory and embryo yielding in sheep using increased exposure time to progesterone associated with a GnRH agonist. Small Ruminant Research, 136, 54–58. https://doi.org/10.1016/j.smallrumres.2016.01.005Bruno-Galarraga, M., Cueto, M., Gibbons, A., Pereyra-Bonnet, F., Subiabre, M., & González-Bulnes, A. (2015). Preselection of high and low ovulatory responders in sheep multiple ovulation and embryo transfer programs. Theriogenology, 84(5), 784–790. https://doi.org/10.1016/j.theriogenology.2015.05.011Caamaño, J. N., Gómez, E., Trigal, B., Muñoz, M., Carrocera, S., Martín, D., & Díez, C. (2015). Survival of vitrified invitro-produced bovine embryos after a one-step warming in-straw cryoprotectant dilution procedure. Theriogenology, 83(5), 881–890. https://doi.org/10.1016/j.theriogenology.2014.11.021Chian, R. C., Son, W. Y., Huang, J. Y., Cui, S. J., Buckett, W. M., & Tan, S. L. (2005). High survival rates and pregnancies of human oocytes following vitrification: preliminary report. Fertility and Sterility, 84, S26, 2005. https://doi.org/https://doi.org/10.1016/j.fertnstert.2005.07.086Cocero, M., Aguilar., B., Alabart, J. L., Olivera, J., & Folch, J. (2000). FACTORES QUE AFECTAN AL RENDIMIENTO DE LA T.E.CONGELADOS EN EL PROGRAMA GENÉTICO DE OVIARAGON. 760–762.Cognie, Y. (1999). State of the art in sheep-goat embryo transfer. Theriogenology, 51(1), 105–116. https://doi.org/10.1016/S0093-691X(98)00235-0 Contreras-Solis, I., Diaz, T., Lopez, G., Caigua, A., Lopez-Sebastian, A., & Gonzalez-Bulnes, A. (2008). Systemic and intraovarian effects of corpus luteum on follicular dynamics during estrous cycle in hair breed sheep. Animal Reproduction Science, 104(1), 47–55. https://doi.org/10.1016/j.anireprosci.2007.01.021Cortés-Reyes, É., Rubio-Romero, J. A., & Gaitán-Duarte, H. (2010). Statistical methods for evaluating diagnostic test agreement and reproducibility. Revista Colombiana de Obstetricia y Ginecologia, 61(3), 247–255. https://doi.org/10.18597/rcog.271Crilly, J. P., Politis, A. P., & Hamer, K. (2017). Use of ultrasonographic examination in sheep veterinary practice. Small Ruminant Research, 152(July), 166–173. https://doi.org/10.1016/j.smallrumres.2016.12.021Cuadro, F., dos Santos-Neto, P. C., Pinczak, A., Barrera, N., Crispo, M., & Menchaca, A. (2018). Serum progesterone concentrations during FSH superstimulation of the first follicular wave affect embryo production in sheep. Animal Reproduction Science, 196, 205–210. https://doi.org/10.1016/j.anireprosci.2018.08.011Cueto, M. I., Gibbons, A. E., Pereyra-Bonnet, F., Silvestre, P., & González-Bulnes, A. (2011a). Effects of Season and Superovulatory Treatment on Embryo Yields in Fine-Wool Merinos Maintained Under Field Conditions. Reproduction in Domestic Animals, 46(5), 770–775. https://doi.org/10.1111/j.1439-0531.2010.01738.xD’Alessandro, A. G., & Martemucci, G. (2016). Superovulatory response to gonadotrophin FSH/LH treatment and effect of progestin supplement to recipients on survival of transferred vitrified embryos in goats. Theriogenology, 85(2), 296–301. https://doi.org/10.1016/j.theriogenology.2015.09.038Dalcin, L., Silva, R. C., Paulini, F., Silva, B. D. M., Neves, J. P., & Lucci, C. M. (2013). Cytoskeleton structure, pattern of mitochondrial activity and ultrastructure of frozen or vitrified sheep embryos. Cryobiology, 67(2), 137–145. https://doi.org/10.1016/j.cryobiol.2013.05.012de Araújo-Lemos, P. F. B., de Freitas Neto, L. M., de Melo, J. V., Moura, M. T., Lima, P. F., & Oliveira, M. a. L. (2014). Comparison of different cryoprotectant regimes for vitrification of ovine embryos produced in vivo. Small Ruminant Research, 119(1–3), 100–106. https://doi.org/10.1016/j.smallrumres.2014.02.013de Araújo-Lemos, P. F. B., Freitas Neto, L. M., Moura, M. T., Melo, J. V., Lima, P. F., & De Oliveira, M. A. L. (2015). Comparison of vitrification and conventional freezing for cryopreservation of caprine embryos. Zygote, 23(4), 594–602. https://doi.org/10.1017/S0967199414000215De Paula, W. B. M., Agip, A. N. A., Missirlis, F., Ashworth, R., Vizcay-Barrena, G., Lucas, C. H., & Allen, J. F. (2013). 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D., Wang, S., & Fuller, B. J. (2017). Cryoprotectants: A review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures. Cryobiology, 76, 74–91. https://doi.org/10.1016/j.cryobiol.2017.04.004Fernandez, J., Bruno- Galarraga, M. M., Soto, A. T., de la Sota, R. L., Cueto, M. I., Lacau, I. M., & Gibbons, A. E. (2018). Hormonal therapeutic strategy on the induction of accessory corpora lutea in relation to follicle size and on the increase of progesterone in sheep. Theriogenology, 105, 184–188. https://doi.org/10.1016/j.theriogenology.2017.09.020Figueira, L. M., Alves, N. G., Batista, R. I. T. P., Brair, V. L., Lima, R. R., Oliveira, M. E. F., Fonseca, J. F., & Souza-Fabjan, J. M. G. (2019). Pregnancy rate after fixed-time transfer of cryopreserved embryos collected by non-surgical route in Lacaune sheep. Reproduction in Domestic Animals, 54(11), 1493–1496. https://doi.org/10.1111/rda.13550Figueira, L. M., Alves, N. G., Maia, A. L. R. e. S., Souza-Fabjan, J. M. G. de, Batista, R. I. T. P., Morais, M. C. da C., Lima, R. R. de, Oliveira, M. E. F., & Fonseca, J. F. da. (2020). Embryo yield and quality are associated with progestogen treatment during superovulation protocol in lactating Lacaune ewes. Theriogenology, 155, 132–138. https://doi.org/10.1016/j.theriogenology.2020.06.004Figueira, L. M., Alves, N. G., Souza-Fabjan, J. M. G., Oliveira, M. E. F., Lima, R. R., Souza, G. N., & Fonseca, J. F. (2020). Preovulatory follicular dynamics, ovulatory response and embryo yield in Lacaune ewes subjected to synchronous estrus induction protocols and non-surgical embryo recovery. In Theriogenology (Vol. 145). Elsevier Inc. https://doi.org/10.1016/j.theriogenology.2019.11.004Fonseca, J. F., Oliveira, M. E. F., Brandão, F. Z., Batista, R. I. T. P., Garcia, A. R., Bartlewski, P. M., & Souza-Fabjan, J. M. G. (2019). Non-surgical embryo transfer in goats and sheep: The Brazilian experience. Reproduction, Fertility and Development, 31(1), 17–26. https://doi.org/10.1071/RD18324Forcada, F., Amer-Meziane, M. A., Abecia, J. A., Maurel, M. C., Cebrián-Pérez, J. A., Muiño-Blanco, T., Asenjo, B., Vázquez, M. I., & Casao, A. (2011). Repeated superovulation using a simplified FSH/eCG treatment for in vivo embryo production in sheep. Theriogenology, 75(4), 769–776. https://doi.org/10.1016/j.theriogenology.2010.10.019Forcada, F., Sánchez-Prieto, L., Casao, A., Palacín, I., Cebrián-Pérez, J. A., Muiño-Blanco, T., & Abecia, J. A. (2012). Use of laparoscopic intrauterine insemination associated with a simplified superovulation treatment for in vivo embryo production in sheep: A preliminary report. Animal Production Science, 52(12), 1111–1116. https://doi.org/10.1071/AN12129Garcia-Dominguez, X., Marco-Jimenez, F., Viudes-de-Castro, M. P., & Vicente, J. S. (2019). Minimally invasive embryo transfer and embryo vitrification at the optimal embryo stage in rabbit model. 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Journal of Animal Science and Biotechnology, 5(1), 1–7. https://doi.org/10.1186/2049-1891-5-19Gobernación de BoyacáColcienciasFundación Universitaria Juan de CastellanosUniversidad Nacional de Colombia Programas de Posgrado de la Facultad de Medicina Veterinaria y de Zootecnia.ORIGINAL2949613899.2020.pdf2949613899.2020.pdfTesis de Maestría en Salud Animalapplication/pdf1193374https://repositorio.unal.edu.co/bitstream/unal/79502/4/2949613899.2020.pdfe11876f2c8866771e397eac3c6e11ba0MD54LICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/79502/2/license.txtcccfe52f796b7c63423298c2d3365fc6MD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805https://repositorio.unal.edu.co/bitstream/unal/79502/3/license_rdf4460e5956bc1d1639be9ae6146a50347MD53THUMBNAIL2949613899.2020.pdf.jpg2949613899.2020.pdf.jpgGenerated 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