Evaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensional
ilustraciones (principalmente a color), diagramas, fotografías
- Autores:
-
Saiz Culma, Juan José
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- eng
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/86500
- Palabra clave:
- 610 - Medicina y salud::612 - Fisiología humana
610 - Medicina y salud::615 - Farmacología y terapéutica
610 - Medicina y salud::616 - Enfermedades
Condrocitos
Cartílago hialino
Terapia por estimulación eléctrica
Glicosaminoglicanos
Ingeniería de tejidos
Regeneración tisular dirigida
Materiales biomiméticos
Chondrocytes
Hyaline cartilage
Electric stimulation therapy
Glycosaminoglycans
Tissue engineering
Guided tissue regeneration
Biomimetic materials
Cartílago hialino
Campos eléctricos
Condrocitos
Proliferación celular
Glicosaminoglicanos
Estímulos biofísicos
Hyaline cartilage
Electric fields
Chondrocytes
Cell proliferation
Glycosaminoglycans
Biophysical stimuli
- Rights
- openAccess
- License
- Reconocimiento 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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|
| dc.title.spa.fl_str_mv |
Evaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensional |
| dc.title.translated.eng.fl_str_mv |
In vitro evaluation of the effect of electrical stimulation on cultured chondrocytes in a three-dimensional construct |
| title |
Evaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensional |
| spellingShingle |
Evaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensional 610 - Medicina y salud::612 - Fisiología humana 610 - Medicina y salud::615 - Farmacología y terapéutica 610 - Medicina y salud::616 - Enfermedades Condrocitos Cartílago hialino Terapia por estimulación eléctrica Glicosaminoglicanos Ingeniería de tejidos Regeneración tisular dirigida Materiales biomiméticos Chondrocytes Hyaline cartilage Electric stimulation therapy Glycosaminoglycans Tissue engineering Guided tissue regeneration Biomimetic materials Cartílago hialino Campos eléctricos Condrocitos Proliferación celular Glicosaminoglicanos Estímulos biofísicos Hyaline cartilage Electric fields Chondrocytes Cell proliferation Glycosaminoglycans Biophysical stimuli |
| title_short |
Evaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensional |
| title_full |
Evaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensional |
| title_fullStr |
Evaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensional |
| title_full_unstemmed |
Evaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensional |
| title_sort |
Evaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensional |
| dc.creator.fl_str_mv |
Saiz Culma, Juan José |
| dc.contributor.advisor.spa.fl_str_mv |
Vaca González, Juan Jairo Garzón-Alvarado, Diego A. |
| dc.contributor.author.spa.fl_str_mv |
Saiz Culma, Juan José |
| dc.contributor.researchgroup.spa.fl_str_mv |
laboratorio de Biomiméticos: Grupo de Mecanobiología de Órganos y Tejidos |
| dc.subject.ddc.spa.fl_str_mv |
610 - Medicina y salud::612 - Fisiología humana 610 - Medicina y salud::615 - Farmacología y terapéutica 610 - Medicina y salud::616 - Enfermedades |
| topic |
610 - Medicina y salud::612 - Fisiología humana 610 - Medicina y salud::615 - Farmacología y terapéutica 610 - Medicina y salud::616 - Enfermedades Condrocitos Cartílago hialino Terapia por estimulación eléctrica Glicosaminoglicanos Ingeniería de tejidos Regeneración tisular dirigida Materiales biomiméticos Chondrocytes Hyaline cartilage Electric stimulation therapy Glycosaminoglycans Tissue engineering Guided tissue regeneration Biomimetic materials Cartílago hialino Campos eléctricos Condrocitos Proliferación celular Glicosaminoglicanos Estímulos biofísicos Hyaline cartilage Electric fields Chondrocytes Cell proliferation Glycosaminoglycans Biophysical stimuli |
| dc.subject.decs.spa.fl_str_mv |
Condrocitos Cartílago hialino Terapia por estimulación eléctrica Glicosaminoglicanos Ingeniería de tejidos Regeneración tisular dirigida Materiales biomiméticos |
| dc.subject.decs.eng.fl_str_mv |
Chondrocytes Hyaline cartilage Electric stimulation therapy Glycosaminoglycans Tissue engineering Guided tissue regeneration Biomimetic materials |
| dc.subject.proposal.spa.fl_str_mv |
Cartílago hialino Campos eléctricos Condrocitos Proliferación celular Glicosaminoglicanos Estímulos biofísicos |
| dc.subject.proposal.eng.fl_str_mv |
Hyaline cartilage Electric fields Chondrocytes Cell proliferation Glycosaminoglycans Biophysical stimuli |
| description |
ilustraciones (principalmente a color), diagramas, fotografías |
| publishDate |
2023 |
| dc.date.issued.none.fl_str_mv |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2024-07-17T00:32:14Z |
| dc.date.available.none.fl_str_mv |
2024-07-17T00:32:14Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/86500 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.repo.none.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/86500 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.references.spa.fl_str_mv |
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Escobar J, Guevara J, et al. Capacitively coupled electrical stimulation of rat chondroepiphysis explants: A histomorphometric analysis. Bioelectrochemistry 2019, vol. 126, pp. 1-11. Vaca-González J. The effect of electric fields on hyaline cartilage: an in vitro and in silico study. Universidad Nacional de Colombia, 2019. |
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Facultad de Medicina |
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Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Vaca González, Juan Jairoc0cf94a51fb9b402f66de8d4af9de08eGarzón-Alvarado, Diego A.3bd3300e515de537404ed24aaad21afe600Saiz Culma, Juan José437fc67a38774208fe957bc0ebbf6d77laboratorio de Biomiméticos: Grupo de Mecanobiología de Órganos y Tejidos2024-07-17T00:32:14Z2024-07-17T00:32:14Z2023https://repositorio.unal.edu.co/handle/unal/86500Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones (principalmente a color), diagramas, fotografíasTreatment of degenerative pathologies of articular cartilage remains a clinical challenge. Biophysical stimuli such as electric fields appear to be a promising non-invasive tool for cartilage tissue restoration. In this in vitro study, the effects of capacitively coupled electric fields were evaluated with an alternating voltage of 50 V (corresponds to 7.7 mV/cm) and 100 V (corresponds to 8.7 mV/cm) with a frequency of 60 kHz during 21 days, on cultured chondrocytes in gelatin hydrogels. Cell quantification and glycosaminoglycan detection were measured from the stimulated and control samples. The results on day 7 showed a reduction in cell proliferation by 24.7% and 39.2% (p < 0.05) in the samples stimulated with electric fields of 7.7 mV /cm and 8.7 mV/cm, respectively. However, on day 7, in the samples stimulated with an electric field of 8.7 mV/cm, an increase of 35.7% (p < 0.05) was obtained with respect to the control in the production of glycosaminoglycans. In conclusion, the results indicate that electrical stimulation has an effect on the synthesis of important molecules that make up the extracellular matrix of hyaline cartilage, such as glycosaminoglycans, which allows to conclude that electric stimulation is a very promising tool to enhance articular cartilage tissue engineering outcomes in therapies that use hydrogels. (Texto tomado de la fuente)El tratamiento de las patologías degenerativas del cartílago hialino sigue siendo un desafío clínico. Los estímulos biofísicos como los campos eléctricos parecen ser una herramienta prometedora no invasiva para la restauración del tejido cartilaginoso. En este estudio in vitro, se evaluaron los efectos de los campos eléctricos acoplados capacitivamente con un voltaje en forma de onda sinusoidal de 50 V (corresponde a 7,7 mV/cm) y 100 V (corresponde a 8,7 mV/cm) con una frecuencia de 60 kHz durante 21 días, en condrocitos cultivados en hidrogeles de gelatina. A partir de las muestras estimuladas y controles, se llevaron a cabo mediciones cuantitativas de proliferación celular y detección de glicosaminoglicanos. Los resultados en el día 7, mostraron una reducción de la proliferación celular en un 24,7% y 39,2% (p < 0,05) en las muestras estimuladas con campos eléctricos de 7,7 mV/cm y 8,7 mV/cm, respectivamente. Sin embargo, en el día 7, en las muestras estimuladas con un campo eléctrico de 8,7 mV/cm, se evidenció un incremento de 35,7% (p < 0,05) con respecto al control en la producción de glicosaminoglicanos. En conclusión, los resultados indican que la estimulación eléctrica tiene un efecto en la síntesis de moléculas importantes que componen la matriz extracelular del cartílago hialino, tales como los glicosaminoglicanos, lo cual permite mejorar los tratamientos que se realizan en la actualidad en ingeniería de tejidos y medicina regenerativa con el fin de recuperar el tejido. (Texto tomado de la fuente)MaestríaMagíster en Ingeniería BiomédicaIngeniería de Tejidosxiv, 58 páginasapplication/pdfengUniversidad Nacional de ColombiaBogotá - Medicina - Maestría en Ingeniería BiomédicaFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::612 - Fisiología humana610 - Medicina y salud::615 - Farmacología y terapéutica610 - Medicina y salud::616 - EnfermedadesCondrocitosCartílago hialinoTerapia por estimulación eléctricaGlicosaminoglicanosIngeniería de tejidosRegeneración tisular dirigidaMateriales biomiméticosChondrocytesHyaline cartilageElectric stimulation therapyGlycosaminoglycansTissue engineeringGuided tissue regenerationBiomimetic materialsCartílago hialinoCampos eléctricosCondrocitosProliferación celularGlicosaminoglicanosEstímulos biofísicosHyaline cartilageElectric fieldsChondrocytesCell proliferationGlycosaminoglycansBiophysical stimuliEvaluación in vitro del efecto de estimulación eléctrica sobre condrocitos cultivados en un constructo tridimensionalIn vitro evaluation of the effect of electrical stimulation on cultured chondrocytes in a three-dimensional constructTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBhosale A, Richardson J. 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