Molecular identification of clinical isolates of Fusarium in Colombia

Objective Identifying Fusarium isolates from mycosis symptomatic patients through molecular techniques as PCR and sequencing.Methods In this study, samples were taken from 101 mycosis symptomatic patients in-between 2004-2006. To determine isolates belonging to the Fusarium genus, the DNAr 28S regio...

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Autores:
Gaviria-Rivera, Adelaida Maria
Giraldo-López, Alejandra
Santa-Cardona, Carolina
Cano-Restrepo, Luz
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/65423
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/65423
http://bdigital.unal.edu.co/66446/
Palabra clave:
36 Problemas y servicios sociales, asociaciones / Social problems and social services
61 Ciencias médicas; Medicina / Medicine and health
PCR
rDNA
sequence analysis
elongation factor
ADN ribosómico
PCR
secuenciación
elongación
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_5ec5adc96572fc7815a78e346f50f9cf
oai_identifier_str oai:repositorio.unal.edu.co:unal/65423
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Molecular identification of clinical isolates of Fusarium in Colombia
title Molecular identification of clinical isolates of Fusarium in Colombia
spellingShingle Molecular identification of clinical isolates of Fusarium in Colombia
36 Problemas y servicios sociales, asociaciones / Social problems and social services
61 Ciencias médicas; Medicina / Medicine and health
PCR
rDNA
sequence analysis
elongation factor
ADN ribosómico
PCR
secuenciación
elongación
title_short Molecular identification of clinical isolates of Fusarium in Colombia
title_full Molecular identification of clinical isolates of Fusarium in Colombia
title_fullStr Molecular identification of clinical isolates of Fusarium in Colombia
title_full_unstemmed Molecular identification of clinical isolates of Fusarium in Colombia
title_sort Molecular identification of clinical isolates of Fusarium in Colombia
dc.creator.fl_str_mv Gaviria-Rivera, Adelaida Maria
Giraldo-López, Alejandra
Santa-Cardona, Carolina
Cano-Restrepo, Luz
dc.contributor.author.spa.fl_str_mv Gaviria-Rivera, Adelaida Maria
Giraldo-López, Alejandra
Santa-Cardona, Carolina
Cano-Restrepo, Luz
dc.subject.ddc.spa.fl_str_mv 36 Problemas y servicios sociales, asociaciones / Social problems and social services
61 Ciencias médicas; Medicina / Medicine and health
topic 36 Problemas y servicios sociales, asociaciones / Social problems and social services
61 Ciencias médicas; Medicina / Medicine and health
PCR
rDNA
sequence analysis
elongation factor
ADN ribosómico
PCR
secuenciación
elongación
dc.subject.proposal.spa.fl_str_mv PCR
rDNA
sequence analysis
elongation factor
ADN ribosómico
PCR
secuenciación
elongación
description Objective Identifying Fusarium isolates from mycosis symptomatic patients through molecular techniques as PCR and sequencing.Methods In this study, samples were taken from 101 mycosis symptomatic patients in-between 2004-2006. To determine isolates belonging to the Fusarium genus, the DNAr 28S region was amplified through PCR and specific PCR primers further confirmed their identity to the species level. Additionally, in order to confirm the identity of the species of the isolates, 75 isolates of these were analyzed by partial sequencing of the 28S rDNA and the TEF1-α gene.Results The 28S rDNA portion detected all 101 isolates as belonging to Fusarium and the PCR specific primers detected 52 and 29 isolates as F. oxysporum and F. solani, respectively; 34 and 41 of these, afterwards studied by partial sequencing of the 28S rDNA and TEF1-α genes respectively, were effectively identified by the technique.Conclusion From all the molecular markers used to identify Fusarium isolates, the sequence of the TEF1-α gene provided the best resolution in the identification of species level; however it is possible to discriminate between F. oxysporum and F. solani isolates by PCR, in most of the cases, what is important considering the simplicity of the technique and a faster diagnosis.
publishDate 2018
dc.date.issued.spa.fl_str_mv 2018-01-01
dc.date.accessioned.spa.fl_str_mv 2019-07-03T00:19:55Z
dc.date.available.spa.fl_str_mv 2019-07-03T00:19:55Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.content.spa.fl_str_mv Text
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format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.issn.spa.fl_str_mv ISSN: 2539-3596
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/65423
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/66446/
identifier_str_mv ISSN: 2539-3596
url https://repositorio.unal.edu.co/handle/unal/65423
http://bdigital.unal.edu.co/66446/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv https://revistas.unal.edu.co/index.php/revsaludpublica/article/view/51923
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista de Salud Pública
Revista de Salud Pública
dc.relation.references.spa.fl_str_mv Gaviria-Rivera, Adelaida Maria and Giraldo-López, Alejandra and Santa-Cardona, Carolina and Cano-Restrepo, Luz (2018) Molecular identification of clinical isolates of Fusarium in Colombia. Revista de Salud Pública, 20 (1). pp. 94-102. ISSN 2539-3596
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina - Instituto de Salud Pública
institution Universidad Nacional de Colombia
bitstream.url.fl_str_mv https://repositorio.unal.edu.co/bitstream/unal/65423/1/51923-389179-1-PB.pdf
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bitstream.checksum.fl_str_mv 434155bfed4e556a970c61d990dd49f4
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repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
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spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Gaviria-Rivera, Adelaida Maria6c56fd42-5cdf-4b75-8d47-456f036278f6300Giraldo-López, Alejandra0049b7e3-d2c3-4ad6-bd64-6187623a4962300Santa-Cardona, Carolina32a23223-6e95-4315-a98e-5b761c49626a300Cano-Restrepo, Luzc5d16c19-c49c-4efe-af5c-5d46b9c275203002019-07-03T00:19:55Z2019-07-03T00:19:55Z2018-01-01ISSN: 2539-3596https://repositorio.unal.edu.co/handle/unal/65423http://bdigital.unal.edu.co/66446/Objective Identifying Fusarium isolates from mycosis symptomatic patients through molecular techniques as PCR and sequencing.Methods In this study, samples were taken from 101 mycosis symptomatic patients in-between 2004-2006. To determine isolates belonging to the Fusarium genus, the DNAr 28S region was amplified through PCR and specific PCR primers further confirmed their identity to the species level. Additionally, in order to confirm the identity of the species of the isolates, 75 isolates of these were analyzed by partial sequencing of the 28S rDNA and the TEF1-α gene.Results The 28S rDNA portion detected all 101 isolates as belonging to Fusarium and the PCR specific primers detected 52 and 29 isolates as F. oxysporum and F. solani, respectively; 34 and 41 of these, afterwards studied by partial sequencing of the 28S rDNA and TEF1-α genes respectively, were effectively identified by the technique.Conclusion From all the molecular markers used to identify Fusarium isolates, the sequence of the TEF1-α gene provided the best resolution in the identification of species level; however it is possible to discriminate between F. oxysporum and F. solani isolates by PCR, in most of the cases, what is important considering the simplicity of the technique and a faster diagnosis.Objetivo Identificar aislamientos de Fusarium en pacientes con micosis por medio de las técnicas moleculares de PCR y secuenciación.Métodos Se tomaron 101 muestras de pacientes con micosis sintomática, entre los años 2004 y 2006. Para la detección de aislamientos como pertenecientes al género Fusarium, se amplificó parcialmente por PCR la región 28S del DNAr; y posteriormente —para la detección de la especie de Fusarium— se utilizaron cebadores específicos para F. oxysporum y F. solani. La verificación de la identidad de la especie de los aislamientos se hizo por secuenciación parcial de los genes 28S DNAr y TEF1-α.Resultados El total de 101 aislamientos fueron detectados como pertenecientes al género Fusarium utilizando un cebador universal de la region 28S DNAr; 52 y 29 aislamientos se detectaron como F. oxysporum o F. salani, respectivamente con los cebadores específicos, y la secuenciación parcial de los genes 28S rDNA o TEF1-α confirmó la identidad de las especies.Conclusión La secuenciación parcial del gen TEF1-α es aún el mejor marcador molecular para identificar aislamientos de Fusarium a nivel de especie. Sin embargo, en la mayoría de los casos es posible discriminar entre aislamientos de F. oxysporum y F. solani por PCR con cebadores específicos, lo que proporciona una ventaja importante considerando la simplicidad de la técnica y el rápido diagnóstico.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina - Instituto de Salud Públicahttps://revistas.unal.edu.co/index.php/revsaludpublica/article/view/51923Universidad Nacional de Colombia Revistas electrónicas UN Revista de Salud PúblicaRevista de Salud PúblicaGaviria-Rivera, Adelaida Maria and Giraldo-López, Alejandra and Santa-Cardona, Carolina and Cano-Restrepo, Luz (2018) Molecular identification of clinical isolates of Fusarium in Colombia. Revista de Salud Pública, 20 (1). pp. 94-102. ISSN 2539-359636 Problemas y servicios sociales, asociaciones / Social problems and social services61 Ciencias médicas; Medicina / Medicine and healthPCRrDNAsequence analysiselongation factorADN ribosómicoPCRsecuenciaciónelongaciónMolecular identification of clinical isolates of Fusarium in ColombiaArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL51923-389179-1-PB.pdfapplication/pdf687221https://repositorio.unal.edu.co/bitstream/unal/65423/1/51923-389179-1-PB.pdf434155bfed4e556a970c61d990dd49f4MD51THUMBNAIL51923-389179-1-PB.pdf.jpg51923-389179-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg7822https://repositorio.unal.edu.co/bitstream/unal/65423/2/51923-389179-1-PB.pdf.jpg8f96958022f33de8dcf14bff20eb637bMD52unal/65423oai:repositorio.unal.edu.co:unal/654232023-05-19 23:47:04.558Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

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