High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i

The present article describes an efficient, simple and unexpensive method for the production of high cellular densities of Haemophilus influenzae for cellular and cell free vaccine production. We present here a liquid culture-based method that had not been successfully implemented in Colombia, so fa...

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Autores:
Zuluaga, D.
Tipo de recurso:
Article of journal
Fecha de publicación:
1998
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/40848
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/40848
http://bdigital.unal.edu.co/30945/
Palabra clave:
Haemophilus influenciae
fermentation
outer membrane antigens
P6 protein
Haemophilus influenzae
Fermentación
Antígenos de membrana externa
proteina P6
Rights
closedAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_5ea92274098fa67fb5db160d657a6dce
oai_identifier_str oai:repositorio.unal.edu.co:unal/40848
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
spelling Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbZuluaga, D.a3cd3ec9-281d-45ac-afb9-ae998e7ac6333002019-06-28T09:42:07Z2019-06-28T09:42:07Z1998https://repositorio.unal.edu.co/handle/unal/40848http://bdigital.unal.edu.co/30945/The present article describes an efficient, simple and unexpensive method for the production of high cellular densities of Haemophilus influenzae for cellular and cell free vaccine production. We present here a liquid culture-based method that had not been successfully implemented in Colombia, so far having given low yields and high costs. The kinetics, costs and production of the most promisory antigen candidates for vaccine production were evaluated in liquid and solid reference media.EI presente artículo describe una metodología eficiente, simple y económica para la obtención de altas densidades celulares de Haemophilus influenzae, con el fin de producir vacunas tanto celulares como acelulares. Se describe un método de cultivo en líquido para este microorganismo, el cual no había sido implementado en el país. Después de varios años de investigación por parte de varios grupos, principalmente debido a los bajos rendimientos obtenidos o a los altos costos de las formulaciones más exitosas. Se evalúa el sistema, con respecto a medios sólidos y líquidos de referencia, teniendo en cuenta la cinética de crecimiento, los costos y la expresión de los antígenos más promisorios como vacuna purificada.spaUniversidad Nacional de Colombiahttp://revistas.unal.edu.co/index.php/biotecnologia/article/view/29989Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de BiotecnologíaRevista Colombiana de BiotecnologíaRevista Colombiana de Biotecnología; Vol. 1, núm. 1 (1998); 69-73 1909-8758 0123-3475Zuluaga, D. (1998) High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i. Revista Colombiana de Biotecnología; Vol. 1, núm. 1 (1998); 69-73 1909-8758 0123-3475 .High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part iArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTHaemophilus influenciaefermentationouter membrane antigensP6 proteinHaemophilus influenzaeFermentaciónAntígenos de membrana externaproteina P6unal/40848oai:repositorio.unal.edu.co:unal/408482021-04-23 10:40:25.899Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co
dc.title.spa.fl_str_mv High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i
title High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i
spellingShingle High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i
Haemophilus influenciae
fermentation
outer membrane antigens
P6 protein
Haemophilus influenzae
Fermentación
Antígenos de membrana externa
proteina P6
title_short High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i
title_full High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i
title_fullStr High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i
title_full_unstemmed High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i
title_sort High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i
dc.creator.fl_str_mv Zuluaga, D.
dc.contributor.author.spa.fl_str_mv Zuluaga, D.
dc.subject.proposal.spa.fl_str_mv Haemophilus influenciae
fermentation
outer membrane antigens
P6 protein
Haemophilus influenzae
Fermentación
Antígenos de membrana externa
proteina P6
topic Haemophilus influenciae
fermentation
outer membrane antigens
P6 protein
Haemophilus influenzae
Fermentación
Antígenos de membrana externa
proteina P6
description The present article describes an efficient, simple and unexpensive method for the production of high cellular densities of Haemophilus influenzae for cellular and cell free vaccine production. We present here a liquid culture-based method that had not been successfully implemented in Colombia, so far having given low yields and high costs. The kinetics, costs and production of the most promisory antigen candidates for vaccine production were evaluated in liquid and solid reference media.
publishDate 1998
dc.date.issued.spa.fl_str_mv 1998
dc.date.accessioned.spa.fl_str_mv 2019-06-28T09:42:07Z
dc.date.available.spa.fl_str_mv 2019-06-28T09:42:07Z
dc.type.spa.fl_str_mv Artículo de revista
dc.type.coar.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_6501
dc.type.coarversion.spa.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/ART
format http://purl.org/coar/resource_type/c_6501
status_str publishedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/40848
dc.identifier.eprints.spa.fl_str_mv http://bdigital.unal.edu.co/30945/
url https://repositorio.unal.edu.co/handle/unal/40848
http://bdigital.unal.edu.co/30945/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.spa.fl_str_mv http://revistas.unal.edu.co/index.php/biotecnologia/article/view/29989
dc.relation.ispartof.spa.fl_str_mv Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Biotecnología
Revista Colombiana de Biotecnología
dc.relation.ispartofseries.none.fl_str_mv Revista Colombiana de Biotecnología; Vol. 1, núm. 1 (1998); 69-73 1909-8758 0123-3475
dc.relation.references.spa.fl_str_mv Zuluaga, D. (1998) High yield fermentation of haemophilus influenzae and protein p6 expression: an approach to the production of a purified vaccine. part i. Revista Colombiana de Biotecnología; Vol. 1, núm. 1 (1998); 69-73 1909-8758 0123-3475 .
dc.rights.spa.fl_str_mv Derechos reservados - Universidad Nacional de Colombia
dc.rights.coar.fl_str_mv http://purl.org/coar/access_right/c_14cb
dc.rights.license.spa.fl_str_mv Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/closedAccess
rights_invalid_str_mv Atribución-NoComercial 4.0 Internacional
Derechos reservados - Universidad Nacional de Colombia
http://creativecommons.org/licenses/by-nc/4.0/
http://purl.org/coar/access_right/c_14cb
eu_rights_str_mv closedAccess
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia
institution Universidad Nacional de Colombia
repository.name.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
repository.mail.fl_str_mv repositorio_nal@unal.edu.co
_version_ 1814090231291314176

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