Estudio de las lectinas presentes en el alga roja Tricleocarpa cylindrica

ilustraciones, fotografías, graficas

Autores:: Duarte Tayo, Angie Patricia

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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oai_identifier_str	oai:repositorio.unal.edu.co:unal/81388
network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Estudio de las lectinas presentes en el alga roja Tricleocarpa cylindrica
dc.title.translated.eng.fl_str_mv	Study of the lectins from the red algae Tricleocarpa cylindrica
title	Estudio de las lectinas presentes en el alga roja Tricleocarpa cylindrica
spellingShingle	Estudio de las lectinas presentes en el alga roja Tricleocarpa cylindrica 570 - Biología::572 - Bioquímica Protectinas EXTRACCION (QUIMICA) Extraction (chemistry) Lectinas Algas marinas Aglutinación Caracterización bioquímica Carbohidratos Interacciones proteína Tricleocarpa cylindrica Lectins Seaweed Agglutination Biochemical characterization Carbohydrates Protein interactions
title_short	Estudio de las lectinas presentes en el alga roja Tricleocarpa cylindrica
title_full	Estudio de las lectinas presentes en el alga roja Tricleocarpa cylindrica
title_fullStr	Estudio de las lectinas presentes en el alga roja Tricleocarpa cylindrica
title_full_unstemmed	Estudio de las lectinas presentes en el alga roja Tricleocarpa cylindrica
title_sort	Estudio de las lectinas presentes en el alga roja Tricleocarpa cylindrica
dc.creator.fl_str_mv	Duarte Tayo, Angie Patricia
dc.contributor.advisor.none.fl_str_mv	Vega Castro, Nohora Angélica Puyana Hegedus, Mónica
dc.contributor.author.none.fl_str_mv	Duarte Tayo, Angie Patricia
dc.contributor.researchgroup.spa.fl_str_mv	Grupo de Investigación en Proteinas Grip
dc.subject.ddc.spa.fl_str_mv	570 - Biología::572 - Bioquímica
topic	570 - Biología::572 - Bioquímica Protectinas EXTRACCION (QUIMICA) Extraction (chemistry) Lectinas Algas marinas Aglutinación Caracterización bioquímica Carbohidratos Interacciones proteína Tricleocarpa cylindrica Lectins Seaweed Agglutination Biochemical characterization Carbohydrates Protein interactions
dc.subject.lemb.spa.fl_str_mv	Protectinas EXTRACCION (QUIMICA)
dc.subject.lemb.eng.fl_str_mv	Extraction (chemistry)
dc.subject.proposal.spa.fl_str_mv	Lectinas Algas marinas Aglutinación Caracterización bioquímica Carbohidratos Interacciones proteína
dc.subject.proposal.none.fl_str_mv	Tricleocarpa cylindrica
dc.subject.proposal.eng.fl_str_mv	Lectins Seaweed Agglutination Biochemical characterization Carbohydrates Protein interactions
description	ilustraciones, fotografías, graficas
publishDate	2021
dc.date.issued.none.fl_str_mv	2021
dc.date.accessioned.none.fl_str_mv	2022-03-25T17:05:54Z
dc.date.available.none.fl_str_mv	2022-03-25T17:05:54Z
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/81388
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/81388 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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(2021). 35 years in plant lectin research: a journey from basic science to applications in agriculture and medicine. Glycoconjugate Journal 2021, 1–15. https://doi.org/10.1007/S10719-021-10015-X Vanderlei, E., Patoilo, K., Lima, N., Lima, A., Rodrigues, J., Silva, L., Lima, M., Lima, V., & Benevides, N. (2010). Antinociceptive and anti-inflammatory activities of lectin from the marine green alga Caulerpa cupressoides. International Immunopharmacology, 10(9), 1113–1118. https://doi.org/10.1016/J.INTIMP.2010.06.014 Varki, A, Etzler, M. E., Cummings, R. D., & Esko, J. D. (2009). Discovery and Classification of Glycan-Binding Proteins. In Essentials of Glycobiology, second edition. Cold Spring Harbor Laboratory Press. https://www.ncbi.nlm.nih.gov/books/NBK1923/ Varki, Ajit, Cummings, R. D., Esko, J. D., Stanley, P., Hart, G. W., Aebi, M., Darvill, A. G., Kinoshita, T., Packer, N. H., Prestegard, J. H., Schnaar, R. L., & Seeberger, P. H. (2017). Essentials of glycobiology, third edition. 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dc.rights.license.spa.fl_str_mv	Atribución-NoComercial 4.0 Internacional
dc.rights.uri.spa.fl_str_mv	http://creativecommons.org/licenses/by-nc/4.0/
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dc.format.extent.spa.fl_str_mv	xvi, 116 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia
dc.publisher.program.spa.fl_str_mv	Bogotá - Ciencias - Maestría en Ciencias - Bioquímica
dc.publisher.department.spa.fl_str_mv	Departamento de Química
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.place.spa.fl_str_mv	Bogotá, Colombia
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Vega Castro, Nohora Angélica1bac60b0381f939db8d6c334f123ddc1Puyana Hegedus, Mónica7c87af3e9533802a30d322ede662b51dDuarte Tayo, Angie Patricia591f029a4d7c7412bddf3593ebfb5473Grupo de Investigación en Proteinas Grip2022-03-25T17:05:54Z2022-03-25T17:05:54Z2021https://repositorio.unal.edu.co/handle/unal/81388Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, fotografías, graficasEl estudio en lectinas de algas es menor comparado con vegetales o animales, sin embargo, se han estudiado principalmente en algas rojas (Phylum Rhodophyta). Por otra parte, la purificación de estas moléculas es dispendiosa dada la cantidad de pigmentos que contienen sus extractos proteicos. En este estudio se llevaron a cabo ensayos de purificación de las lectinas del alga roja Tricleocarpa cylindrica, dado que previamente se detectó su actividad aglutinante; por otra parte, se ha reportado que estas lectinas tienen diferentes actividades biológicas que las postulan como moléculas de gran interés farmacológico. Inicialmente se realizó un tratamiento con acetona a la harina del alga para eliminar interferentes que producen hemólisis de eritrocitos, por precipitación con sulfato de amonio se detectó únicamente actividad aglutinante en la fracción del 50-80%s, los ensayos de inhibición con carbohidratos mostraron reconocimiento principalmente por D-glucosa. Aglutinó preferentemente eritrocitos de humano tipo B con cantidades de 0.6 g de proteína pura, similar a lo reportado para lectinas de otras especies de algas rojas. La lectina de Tricleocarpa cylindrica (LTC) se purificó con una combinación de métodos cromatográficos, que incluyeron intercambio iónico, afinidad y un paso final con HPLC. Esta nueva proteína se caracterizó por ser una glicoproteína con un peso molecular de 29 kDa por SDS PAGE, un punto isoeléctrico ácido (5.7) y alta estabilidad en un rango de pH de 3.0-7.4 y temperatura hasta los 58ºC. (Texto tomado de la fuente)Lectins from marine algae have not been studied such as vegetables or animals, even though, their study is centered in red algae (Phylum Rhodophyta). Furthermore, purification of these proteins is difficult due to pigments in saline extracts. In this study, purification of the lectins from the red algae Tricleocarpa cylindrica were carried out because hemagglutination activity was detected previously; additionally, these lectins could be molecules of great pharmacological potential for its various biological activities. First, removal of pigments was achieved by washing the algae flour with cool acetone to avoid hemolysis, precipitation assays from PBS extracts showed that the whole lectin was obtained in the fractions precipitated with ammonium sulphate 50-80%s. The lectin was able to agglutinate human RBCs from B and O donors; although it agglutinated preferentially B erythrocytes with a minimum lectin quantity of 0.6 g, like other species studied. Several purification procedures were assayed which included DEAE-sephadex, affinity chromatography, and the last step on HPLC. It led to obtaining a pure lectin having high specific agglutination activity (SAA). The PI showed a unique band at pH 5.7, in SDS-PAGE a band around 29 kDa, thermal stability (58 0C), and activity in the pH range 3.0-7.4.MaestríaMagíster en Ciencias - BioquímicaEstudios de Lectinas de algas marinas del Caribe Colombianoxvi, 116 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaDepartamento de QuímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::572 - BioquímicaProtectinasEXTRACCION (QUIMICA)Extraction (chemistry)LectinasAlgas marinasAglutinaciónCaracterización bioquímicaCarbohidratosInteracciones proteínaTricleocarpa cylindricaLectinsSeaweedAgglutinationBiochemical characterizationCarbohydratesProtein interactionsEstudio de las lectinas presentes en el alga roja Tricleocarpa cylindricaStudy of the lectins from the red algae Tricleocarpa cylindricaTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAlam, T. (2018). Extraction of Natural Colors from Marine Algae. Journal of Agricultural and Marine Sciences, 23, 81–91. https://doi.org/10.24200/jams.vol23iss1pp81-91Alexandre, K. B., Gray, E. S., Lambson, B. E., Moore, P. L., Choge, I. A., Mlisana, K., Karim, S. S. A., McMahon, J., O’Keefe, B., Chikwamba, R., & Morris, L. (2010). Mannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin. Virology, 402(1), 187–196. https://doi.org/10.1016/J.VIROL.2010.03.021Alpuche, J., Pereyra, A., Agundis, C., Rosas, C., Pascual, C., Slomianny, M. C., Vázquez, L., & Zenteno, E. (2005). Purification and characterization of a lectin from the white shrimp Litopenaeus setiferus (Crustacea decapoda) hemolymph. Biochimica et Biophysica Acta (BBA) - General Subjects, 1724(1–2), 86–93. https://doi.org/10.1016/J.BBAGEN.2005.04.014Anam, C., Chasanah, E., Perdhana, B. P., Fajarningsih, N. D., Yusro, N. F., Sari, A. 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Protein Science : A Publication of the Protein Society, 16(7), 1485. https://doi.org/10.1110/PS.072889407ORIGINAL1018479298.2021.pdf1018479298.2021.pdfTesis de Maestría en Bioquímicaapplication/pdf2901622https://repositorio.unal.edu.co/bitstream/unal/81388/3/1018479298.2021.pdfb6e031920f4c7ec1e443eeac7d762146MD53LICENSElicense.txtlicense.txttext/plain; charset=utf-84074https://repositorio.unal.edu.co/bitstream/unal/81388/4/license.txt8153f7789df02f0a4c9e079953658ab2MD54THUMBNAIL1018479298.2021.pdf.jpg1018479298.2021.pdf.jpgGenerated Thumbnailimage/jpeg4299https://repositorio.unal.edu.co/bitstream/unal/81388/5/1018479298.2021.pdf.jpgc7cf64068048a0110447b604a9f4de9cMD55unal/81388oai:repositorio.unal.edu.co:unal/813882024-08-05 23:10:22.612Repositorio Institucional Universidad Nacional de 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EVESURBIFBPUiBMQSBTRUNSRVRBUsONQSBHRU5FUkFMLiAqTEEgVEVTSVMgQSBQVUJMSUNBUiBERUJFIFNFUiBMQSBWRVJTScOTTiBGSU5BTCBBUFJPQkFEQS4gCgpBbCBoYWNlciBjbGljIGVuIGVsIHNpZ3VpZW50ZSBib3TDs24sIHVzdGVkIGluZGljYSBxdWUgZXN0w6EgZGUgYWN1ZXJkbyBjb24gZXN0b3MgdMOpcm1pbm9zLiBTaSB0aWVuZSBhbGd1bmEgZHVkYSBzb2JyZSBsYSBsaWNlbmNpYSwgcG9yIGZhdm9yLCBjb250YWN0ZSBjb24gZWwgYWRtaW5pc3RyYWRvciBkZWwgc2lzdGVtYS4KClVOSVZFUlNJREFEIE5BQ0lPTkFMIERFIENPTE9NQklBIC0gw5psdGltYSBtb2RpZmljYWNpw7NuIDE5LzEwLzIwMjEK

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