Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia
ilustraciones, diagramas
- Autores:
-
Osorio Certuche, Nicole
- Tipo de recurso:
- Fecha de publicación:
- 2023
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/84264
- Palabra clave:
- Infección latente
Latent Infection
Pseudomonas aeruginosa
Factores de virulencia
Infecciones Asociadas a la Atención en Salud
Secuencio-tipos
Virulence factors
Healthcare-associated infections
Sequence-types
WGS
- Rights
- openAccess
- License
- Atribución-NoComercial 4.0 Internacional
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UNACIONAL2 |
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Universidad Nacional de Colombia |
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|
| dc.title.spa.fl_str_mv |
Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia |
| dc.title.translated.eng.fl_str_mv |
Genomic characterization of virulence factors of clinical isolates of Pseudomonas aeruginosa based on WGS from a hospital in Bogotá, Colombia |
| title |
Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia |
| spellingShingle |
Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia Infección latente Latent Infection Pseudomonas aeruginosa Factores de virulencia Infecciones Asociadas a la Atención en Salud Secuencio-tipos Virulence factors Healthcare-associated infections Sequence-types WGS |
| title_short |
Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia |
| title_full |
Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia |
| title_fullStr |
Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia |
| title_full_unstemmed |
Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia |
| title_sort |
Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia |
| dc.creator.fl_str_mv |
Osorio Certuche, Nicole |
| dc.contributor.advisor.none.fl_str_mv |
Barreto Hernández, Emiliano Leal Castro, Aura Lucía |
| dc.contributor.author.none.fl_str_mv |
Osorio Certuche, Nicole |
| dc.contributor.researchgroup.spa.fl_str_mv |
Epidemiologia molecular Bioinformática Enfermedades infecciosas |
| dc.subject.decs.spa.fl_str_mv |
Infección latente |
| topic |
Infección latente Latent Infection Pseudomonas aeruginosa Factores de virulencia Infecciones Asociadas a la Atención en Salud Secuencio-tipos Virulence factors Healthcare-associated infections Sequence-types WGS |
| dc.subject.decs.eng.fl_str_mv |
Latent Infection |
| dc.subject.proposal.spa.fl_str_mv |
Pseudomonas aeruginosa Factores de virulencia Infecciones Asociadas a la Atención en Salud Secuencio-tipos |
| dc.subject.proposal.eng.fl_str_mv |
Virulence factors Healthcare-associated infections Sequence-types WGS |
| description |
ilustraciones, diagramas |
| publishDate |
2023 |
| dc.date.accessioned.none.fl_str_mv |
2023-07-25T16:58:01Z |
| dc.date.available.none.fl_str_mv |
2023-07-25T16:58:01Z |
| dc.date.issued.none.fl_str_mv |
2023-01-30 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/84264 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/84264 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
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spa |
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spa |
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(2020). resistencia bacteriana y producción de biopelículas en la ciudad de Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D . C . T . -Colombia. In Universidad de cartagena. Vives-Flórez, M., & Garnica, D. (2006). Comparison of virulence between clinical and environmental Pseudomonas aeruginosa isolates. International Microbiology, 9(4), 247–252. https://doi.org/10.2436/im.v9i4.9582 Wick, R. R., Judd, L. M., Gorrie, C. L., & Holt, K. E. (2017). Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLOS Computational Biology, 13(6), e1005595. https://doi.org/10.1371/JOURNAL.PCBI.1005595 World Health Organization. (2011). Report on the burden of endemic health care-associated infection worldwide: Clean care is safer care. 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Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Barreto Hernández, Emilianob7a2cae2c08b5d6a549e173576c6c82dLeal Castro, Aura Lucíaf07a334eb72fd9cef067a37ca1553060Osorio Certuche, Nicolef5a629880b839bc70ba23eb42d243a1fEpidemiologia molecular Bioinformática Enfermedades infecciosas2023-07-25T16:58:01Z2023-07-25T16:58:01Z2023-01-30https://repositorio.unal.edu.co/handle/unal/84264Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasEn humanos la infección por P. aeruginosa es controlada por múltiples factores de virulencia y es una de las causas más recurrentes y graves de infecciones asociadas a la atención en salud. Por lo tanto, el objetivo general de este estudio fue caracterizar los perfiles genómicos de virulencia en aislados clínicos de Pseudomonas aeruginosa provenientes de un hospital universitario de Bogotá- Colombia, utilizando la tecnología de secuenciación de genoma completo (WGS). Este estudio prospectivo se realizó durante los años 2019 y 2021, en donde se secuenciaron 54 aislamientos provenientes de 37 pacientes de los servicios de UCI, hospitalización y Unidad quirúrgica, utilizando las plataformas illumina y Oxford Nanopore. En los genomas se encontraron un total de 246 genes de virulencia, encontrando la presencia de genes de gran importancia en la virulencia de esta bacteria, como el gen pilA que se detectó en el 48,1% de los aislamientos y el gen algD en el 100%. Para las toxinas la prevalencia fue para exoU de (16,6%), exoT (92,5%), exoS (79,6%) y exoY (88,8%). Adicionalmente se encontró la presencia de 16 secuencio-tipos (ST) ya reportados y se encontraron 13 ST nuevos. En conclusión, el uso de tecnologías como WGS permitió determinar el perfil de virulencia de aislados clínicos de P. aeruginosa, logrando un acercamiento global a los perfiles de virulencia de los aislamientos clínicos de esta bacteria en el país, siendo el primer reporte de la prevalencia de más de 200 genes de virulencia en Colombia para P. aeruginosa. (Texto tomado de la fuente)In humans, P. aeruginosa infection is controlled by multiple virulence factors and is one of the most recurrent and serious causes of healthcare-associated infections. The aim of this study was to characterize the virulence genomic profiles in clinical isolates of Pseudomonas aeruginosa from a hospital in Bogotá-Colombia, using whole genome sequencing (WGS) technology. A prospective study was carried out during the years 2019 and 2021, where 54 isolates from 37 patients from both the ICU and hospitalization services, and operating rooms were sequenced, using the illumina and Oxford Nanopore platforms. A total of 246 virulence genes were found in the genomes, finding the presence of genes of great importance in the virulence of this bacterium, such as the pilA gene that was detected in 48.1% of the isolates and the algD gene that found in the 100% of them. For toxins, the prevalence was for exoU de (16.6%), exoT (92.5%), exoS (79.6%) and exoY (88.8%). Additionally, the presence of 16 sequence-types (ST) already reported and 13 new ST were found. In conclusion, the use of technologies such as WGS made it possible to determine the virulence profile of clinical isolates of P. aeruginosa, achieving a global approach to the virulence profiles of the clinical isolates of this bacterium in the country, being the first report of the prevalence of more than 200 virulence genes in Colombia for P. aeruginosa.MaestríaMagíster en Ciencias - MicrobiologíaBiología molecular de agentes infecciososXI. 79 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogotá,ColombiaUniversidad Nacional de Colombia - Sede BogotáCaracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, ColombiaGenomic characterization of virulence factors of clinical isolates of Pseudomonas aeruginosa based on WGS from a hospital in Bogotá, ColombiaTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMBogotáColombiaAslani, M. 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Journal of Clinical Laboratory Analysis, 35(2), 1–6. https://doi.org/10.1002/jcla.23627Infección latenteLatent InfectionPseudomonas aeruginosaFactores de virulenciaInfecciones Asociadas a la Atención en SaludSecuencio-tiposVirulence factorsHealthcare-associated infectionsSequence-typesWGSLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/84264/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1115086252_2023.pdf1115086252_2023.pdfTesis de Maestría en Ciencias - Microbiologíaapplication/pdf3137609https://repositorio.unal.edu.co/bitstream/unal/84264/2/1115086252_2023.pdf9637116c15f6f30bfdd4e0304ce692b0MD52THUMBNAIL1115086252_2023.pdf.jpg1115086252_2023.pdf.jpgGenerated Thumbnailimage/jpeg4182https://repositorio.unal.edu.co/bitstream/unal/84264/3/1115086252_2023.pdf.jpg9d82d1d3b9745750eb6a9fb766bcda7aMD53unal/84264oai:repositorio.unal.edu.co:unal/842642023-08-13 23:03:45.627Repositorio Institucional Universidad Nacional de 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