Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectados
ilustraciones, diagramas, fotografías
- Autores:
-
Lasso Delgado, Jose Manuel
- Tipo de recurso:
- Fecha de publicación:
- 2024
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/86036
- Palabra clave:
- 590 - Animales::599 - Mamíferos
570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales
Patología animal
animal pathology
Oligodendroglía/patología
Moquillo/fisiopatología
Oligodendroglia/pathology
Distemper/physiopathology
Distemper canino
Oligodendrocitos
Desmielinización
Apoptosis
Caspasa 3
Caspasa 9
Canine distemper
Oligodendrocytes
Demyelination
Apoptosis
Caspase 3
Caspase 9
- Rights
- openAccess
- License
- Reconocimiento 4.0 Internacional
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| dc.title.spa.fl_str_mv |
Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectados |
| dc.title.translated.eng.fl_str_mv |
Evaluation of oligodendrocyte death caused by canine distemper virus in naturally infected animal brain |
| title |
Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectados |
| spellingShingle |
Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectados 590 - Animales::599 - Mamíferos 570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales Patología animal animal pathology Oligodendroglía/patología Moquillo/fisiopatología Oligodendroglia/pathology Distemper/physiopathology Distemper canino Oligodendrocitos Desmielinización Apoptosis Caspasa 3 Caspasa 9 Canine distemper Oligodendrocytes Demyelination Apoptosis Caspase 3 Caspase 9 |
| title_short |
Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectados |
| title_full |
Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectados |
| title_fullStr |
Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectados |
| title_full_unstemmed |
Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectados |
| title_sort |
Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectados |
| dc.creator.fl_str_mv |
Lasso Delgado, Jose Manuel |
| dc.contributor.advisor.spa.fl_str_mv |
Arboleda Bustos, Gonzalo Humberto Botero Espinosa, Lucia |
| dc.contributor.author.spa.fl_str_mv |
Lasso Delgado, Jose Manuel |
| dc.contributor.researchgroup.spa.fl_str_mv |
Muerte Celular |
| dc.subject.ddc.spa.fl_str_mv |
590 - Animales::599 - Mamíferos 570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales |
| topic |
590 - Animales::599 - Mamíferos 570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animales Patología animal animal pathology Oligodendroglía/patología Moquillo/fisiopatología Oligodendroglia/pathology Distemper/physiopathology Distemper canino Oligodendrocitos Desmielinización Apoptosis Caspasa 3 Caspasa 9 Canine distemper Oligodendrocytes Demyelination Apoptosis Caspase 3 Caspase 9 |
| dc.subject.agrovoc.spa.fl_str_mv |
Patología animal |
| dc.subject.agrovoc.eng.fl_str_mv |
animal pathology |
| dc.subject.decs.spa.fl_str_mv |
Oligodendroglía/patología Moquillo/fisiopatología |
| dc.subject.decs.eng.fl_str_mv |
Oligodendroglia/pathology Distemper/physiopathology |
| dc.subject.proposal.spa.fl_str_mv |
Distemper canino Oligodendrocitos Desmielinización Apoptosis Caspasa 3 Caspasa 9 |
| dc.subject.proposal.eng.fl_str_mv |
Canine distemper Oligodendrocytes Demyelination Apoptosis Caspase 3 Caspase 9 |
| description |
ilustraciones, diagramas, fotografías |
| publishDate |
2024 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-06T20:50:34Z |
| dc.date.available.none.fl_str_mv |
2024-05-06T20:50:34Z |
| dc.date.issued.none.fl_str_mv |
2024-02-01 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/86036 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/86036 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
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spa |
| dc.relation.references.spa.fl_str_mv |
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Prevalencia del virus del Distemper Canino en perros (Canis lupus familiaris) de Risaralda, Colombia. In Prevalencia del virus del Distemper Canino en perros (Canis lupus familiaris) de Risaralda, Colombia (Issue 2). Universidad Tecnológica de Pereira, Facultad de Ciencias de la Salud, Medicina Veterinaria y Zootecnia. Rohowsky-Kochan, C., Davidow, A., Dowling, P., & Cook, S. D. (2021). Increased frequency of canine distemper virus-specific antibodies in multiple sclerosis. Brain and Behavior, 11(1), 1–9. https://doi.org/10.1002/brb3.1920 Saito, T. B., Alfieri, A. A., Wosiacki, S. R., Negrão, F. J., Morais, H. S. A., & Alfieri, A. F. (2006). Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis. Research in Veterinary Science, 80(1), 116–119. https://doi.org/10.1016/j.rvsc.2005.03.002 Schmittgen, T. D., & Livak, K. J. (2008). Analyzing real-time PCR data by the comparative CT method. 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Nature Reviews Molecular Cell Biology. https://doi.org/10.1038/s41580-023-00689-6 Zacarias, J., Dimande, A., Achá, S., Dias, P. T., Leonel, E. M., Messa, A., Macucule, B., Júnior, J. L., & Bila, C. G. (2016). Severe canine distemper outbreak in unvaccinated dogs in Mozambique. Journal of the South African Veterinary Association, 87(1), e1-2. https://doi.org/10.4102/jsava.v87i1.1350 Zaqout, S., Becker, L. L., & Kaindl, A. M. (2020). Immunofluorescence Staining of Paraffin Sections Step by Step. Frontiers in Neuroanatomy, 14(November), 1–11. https://doi.org/10.3389/fnana.2020.582218 Zhao, J., & Ren, Y. (2022). Multiple Receptors Involved in Invasion and Neuropathogenicity of Canine Distemper Virus: A Review. Viruses, 14(7). https://doi.org/10.3390/v14071520 Zurbriggen, A., Schmid, I., Graber, H. U., & Vandevelde, M. (1997). Oligodendroglial pathology in canine distemper. 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Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Arboleda Bustos, Gonzalo Humberto8d01100986b4e816de54bb4d3f52f1f0Botero Espinosa, Lucia9feb4b2c171f4e4975d9195bd3f56cf9600Lasso Delgado, Jose Manuel790e0f779ee73f31a856efabb12041ccMuerte Celular2024-05-06T20:50:34Z2024-05-06T20:50:34Z2024-02-01https://repositorio.unal.edu.co/handle/unal/86036Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramas, fotografíasEl Distemper Canino (CDV) es un virus de la familia Paramixoviridae y género Morbillivirus que afecta a caninos y otros mamíferos carnívoros, causando la enfermedad conocida como Distemper canino (DC) o "Moquillo canino". Este virus, se considera inmunosupresor y puede inducir la muerte de células inmunitarias, facilitando infecciones secundarias. En su fase neurológica, considerada terminal, provoca desmielinización en el sistema nervioso central (SNC) y periférico (SNP). Aunque la apoptosis se ha relacionado con la muerte celular en el SNC, se desconoce el mecanismo molecular de muerte celular específica en células mielinizantes (oligodendrocitos). Metodología: Se diseño un estudio descriptivo transversal cuyo objetivo fue dilucidar si la apoptosis participa como mecanismo de muerte de oligodendrocitos en la enfermedad desmielinizante causada por CDV en caninos infectados naturalmente. Se evaluaron muestras parafinadas cerebrales y cerebelares postmortem de 17 caninos positivos para CDV y 10 caninos control de la colección del Laboratorio de Patología Animal de la Universidad Nacional de Colombia. En estas, se buscó determinar: 1) la presencia y activación de proteínas proapoptóticas (Caspasa 3 clivada, Caspasa 9 clivada) en áreas desmielinizadas de cerebro y cerebelo a través de inmunofluorescencia. y 2) la presencia viral y expresión de genes proapoptóticos (Casp3, Casp9, BAX) y antiapoptóticos (BCL-2) mediante reacción en cadena de la polimerasa en tiempo real por transcriptasa inversa (RT-qPCR). Resultados: En la inmunofluorescencia se evidenció un aumento significativo de la activación y presencia de proteínas proapoptóticas (Caspasa 3 clivada: p = <0.0001, Caspasa 9 clivada: p = <0.0001) en áreas de desmielinización tanto en corteza como en cerebelo de los animales caso con respecto a animales control. Por otra parte, los resultados de los RT-qPCR arrojaron resultados significativos en la expresión de caspasa 3 (p = 0.0297), pero no mostraron cambios significativos en los demás genes proapoptóticos como caspasa 9 (p = 0.4437) y BAX (p = 0.6134), ni antiapoptóticos como BCL-2 (p = 0.3910). Estos hallazgos sugieren una asociación directa entre el virus del distemper canino y la apoptosis de los oligodendrocitos, destacando la complejidad de las respuestas celulares y genéticas involucradas en la patogénesis de esta enfermedad. Sin embargo existen limitantes en el estudio como el tamaño de la muestra que pueden afectar los resultados de forma significativa, además del uso de muestras parafinadas que pueden afectar la extracción y los índices de calidad de los ácidos nucleicos de la muestra de RNA para el RT-qPCR. (Texto tomado de la fuente).Canine Distemper (CDV) is a virus belonging to the Paramyxoviridae family and Morbillivirus genus that affects canines and other carnivorous mammals, causing the disease known as Canine Distemper (CD) or "Canine Distemper." This virus is considered immunosuppressive and can induce the death of immune cells, facilitating secondary infections. In its neurological phase, considered terminal, it causes demyelination in the central and peripheral nervous systems (CNS). Although apoptosis has been linked to cell death in the CNS, the specific molecular mechanism of cell death in myelinating cells (oligodendrocytes) is unknown. Methodology: A descriptive cross-sectional study was designed to elucidate whether apoptosis participates as a mechanism of oligodendrocyte death in the demyelinating disease caused by CDV in naturally infected canines. Postmortem brain and cerebellum paraffin samples from 17 CDV-positive canines and 10 control canines from the Animal Pathology Laboratory collection at the National University of Colombia were evaluated. The study aimed to determine: 1) the presence and activation of proapoptotic proteins (cleaved Caspase 3, cleaved Caspase 9) in demyelinated areas of the brain and cerebellum through immunofluorescence, and 2) the viral presence and expression of proapoptotic genes (Casp3, Casp9, Bax) and antiapoptotic genes (Bcl-2) through real-time reverse transcription polymerase chain reaction (RT-qPCR). Results: Immunofluorescence revealed a significant increase in the activation and presence of proapoptotic proteins (cleaved Caspase 3: p < 0.0001, cleaved Caspase 9: p < 0.0001) in demyelinated areas in both the cortex and cerebellum of case animals compared to control animals. On the other hand, RT-qPCR results showed significant outcomes in the expression of caspase 3 (p = 0.0297), but no significant changes in other proapoptotic genes such as caspase 9 (p = 0.4437) and BAX (p = 0.6134), nor in antiapoptotic gene BCL-2 (p = 0.3910). These findings suggest a direct association between the canine distemper virus and apoptosis of oligodendrocytes, highlighting the complexity of cellular and genetic responses involved in the pathogenesis of this disease. However, there are limitations in the study, such as the sample size, which could significantly impact results, and the use of paraffin samples, which may affect the extraction and purity of RNA for RT-qPCR.MaestríaMagíster en NeurocienciasSe diseñó un estudio descriptivo transversal en el cual se tomaron muestras de SNC (cerebrales y cerebelares) de necropsias de caninos con casos compatibles con DC, pertenecientes a la colección del Laboratorio de Patología Animal adscrito a la FMVZ-UNAL, para correlacionar los hallazgos histopatológicos y moleculares con procesos de muerte celular en la enfermedad desmielinizante causada por la infección del CDV, con el objetivo de analizar el mecanismo de muerte de oligodendrocitos que ocurre en la enfermedad desmielinizante del SNC en pacientes caninos naturalmente infectados por el virus y fallecidos por estas causas.Neurobiologia celular y molecularxiii, 81 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Medicina - Maestría en NeurocienciasFacultad de MedicinaBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá590 - Animales::599 - Mamíferos570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animalesPatología animalanimal pathologyOligodendroglía/patologíaMoquillo/fisiopatologíaOligodendroglia/pathologyDistemper/physiopathologyDistemper caninoOligodendrocitosDesmielinizaciónApoptosisCaspasa 3Caspasa 9Canine distemperOligodendrocytesDemyelinationApoptosisCaspase 3Caspase 9Evaluación de la muerte de oligodendrocitos causada por el virus de distemper canino en cerebro de animales naturalmente infectadosEvaluation of oligodendrocyte death caused by canine distemper virus in naturally infected animal brainTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAige, V., & Morales, C. 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Journal of the Neurological Sciences, 79(1–2), 33–41. https://doi.org/10.1016/0022-510X(87)90257-7EstudiantesInvestigadoresMaestrosPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86036/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1085280935.2024.pdf1085280935.2024.pdfTesis de Maestría en Neurocienciasapplication/pdf3147814https://repositorio.unal.edu.co/bitstream/unal/86036/2/1085280935.2024.pdf2605c8b335c978c1645a04894b8e0841MD52THUMBNAIL1085280935.2024.pdf.jpg1085280935.2024.pdf.jpgGenerated Thumbnailimage/jpeg4845https://repositorio.unal.edu.co/bitstream/unal/86036/3/1085280935.2024.pdf.jpg10b215a98ad82f6af53be9cf0e48d75aMD53unal/86036oai:repositorio.unal.edu.co:unal/860362024-05-06 23:04:44.767Repositorio Institucional Universidad Nacional de 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