Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.

ilustraciones

Autores:: Sepúlveda Correa, Alejandro

Tipo de recurso:

Fecha de publicación:: 2021

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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oai_identifier_str	oai:repositorio.unal.edu.co:unal/79917
network_acronym_str	UNACIONAL2
network_name_str	Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv	Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.
dc.title.translated.eng.fl_str_mv	Metagenomic characterization of genes associated with the synthesis and resistance of antimicrobial compounds in mangrove soils.
title	Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.
spellingShingle	Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar. 500 - Ciencias naturales y matemáticas 630 - Agricultura y tecnologías relacionadas::634 - Huertos, frutas, silvicultura Actinobacterias Aguas residuales Manglares (Ecología) Manglares Metabolitos secundarios Resistencia antimicrobiana Ambientes costeros áridos Ecología microbiana Microbial ecology Secondary metabolites Antibiotics resistance Arid coastal environments
title_short	Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.
title_full	Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.
title_fullStr	Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.
title_full_unstemmed	Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.
title_sort	Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.
dc.creator.fl_str_mv	Sepúlveda Correa, Alejandro
dc.contributor.advisor.none.fl_str_mv	Polanía, Jaime Vanegas Guerrero, Javier
dc.contributor.author.none.fl_str_mv	Sepúlveda Correa, Alejandro
dc.subject.ddc.spa.fl_str_mv	500 - Ciencias naturales y matemáticas 630 - Agricultura y tecnologías relacionadas::634 - Huertos, frutas, silvicultura
topic	500 - Ciencias naturales y matemáticas 630 - Agricultura y tecnologías relacionadas::634 - Huertos, frutas, silvicultura Actinobacterias Aguas residuales Manglares (Ecología) Manglares Metabolitos secundarios Resistencia antimicrobiana Ambientes costeros áridos Ecología microbiana Microbial ecology Secondary metabolites Antibiotics resistance Arid coastal environments
dc.subject.other.none.fl_str_mv	Actinobacterias
dc.subject.lemb.none.fl_str_mv	Aguas residuales Manglares (Ecología)
dc.subject.proposal.spa.fl_str_mv	Manglares Metabolitos secundarios Resistencia antimicrobiana Ambientes costeros áridos
dc.subject.proposal.eng.fl_str_mv	Ecología microbiana Microbial ecology Secondary metabolites Antibiotics resistance Arid coastal environments
description	ilustraciones
publishDate	2021
dc.date.accessioned.none.fl_str_mv	2021-08-11T16:38:37Z
dc.date.available.none.fl_str_mv	2021-08-11T16:38:37Z
dc.date.issued.none.fl_str_mv	2021
dc.type.spa.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv	Text
dc.type.redcol.spa.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/79917
dc.identifier.instname.spa.fl_str_mv	Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv	Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv	https://repositorio.unal.edu.co/
url	https://repositorio.unal.edu.co/handle/unal/79917 https://repositorio.unal.edu.co/
identifier_str_mv	Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv	spa
language	spa
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dc.rights.license.spa.fl_str_mv	Atribución-NoComercial-SinDerivadas 4.0 Internacional
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dc.format.extent.spa.fl_str_mv	68 páginas
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia Sede Medellin
dc.publisher.program.spa.fl_str_mv	Medellín - Ciencias Agrarias - Maestría en Bosques y Conservación Ambiental
dc.publisher.department.spa.fl_str_mv	Departamento de Ciencias Forestales
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias Agrarias
dc.publisher.place.spa.fl_str_mv	Medellín
dc.publisher.branch.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Medellín
institution	Universidad Nacional de Colombia
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spelling	Atribución-NoComercial-SinDerivadas 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Polanía, Jaimec75ee7ba70944e2cd7ab8f8d3b570234Vanegas Guerrero, Javier5c355b1928b3152d824b75fcd7631f93600Sepúlveda Correa, Alejandro01353ad1c0a3e033b2d2fc679c6ec1e02021-08-11T16:38:37Z2021-08-11T16:38:37Z2021https://repositorio.unal.edu.co/handle/unal/79917Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustracionesLos manglares responden a factores bióticos y abióticos, como la salinidad variable y el estrés antropogénico (por ejemplo, la contaminación por desechos urbanos). Esos factores también pueden afectar a las poblaciones de microorganismos e incluso a la relativa abundancia de genes que intervienen en las respuestas biológicas. Esta investigación tenía por objeto identificar los genes asociados con la resistencia y la biosíntesis de los compuestos antimicrobianos en los suelos de manglares sometidos a salinidades contrastantes. El ADN total se extrajo de muestras de suelo rizosférico de tres áreas con diferentes salinidades en un manglar contaminado con aguas residuales en la desembocadura del río Ranchería (La Guajira, Colombia) para su secuenciamiento mediante Illumina HiSeq 2500. El análisis de la diversidad funcional y taxonómica reveló un dominio de 51 familias y 182 géneros de actinobacterias. Las vías de biosíntesis más comunes fueron para la estreptomicina y los monobactámicos. Fue posible asociar 43 genes relacionados con la síntesis de compuestos antimicrobianos, y la abundancia de 24 de ellos se vio considerablemente influida por la salinidad. El aumento de la concentración de sal influyó en las vías metabólicas y en la abundancia diferencial de los genes asociados con la síntesis de compuestos antimicrobianos (por ejemplo, rfbB / rffG, INO1 / ISYNA1, rfbA / rffH, sat / met3, asd, lysC, proA, aspB, fabG). Además, de los 29 genes implicados en la resistencia intrínseca a los antibióticos, 16 estaban influidos significativamente por la salinidad (por ejemplo, aacA, oleC4, oleC5, vgb, acrB / mexB / adeJ / smeE / mtrD / cmeB, bpeF, mexF). Se concluye que los mecanismos de tolerancia y adaptabilidad a las condiciones de estrés salino favorecerían la síntesis de compuestos antimicrobianos en los manglares sujetos a contaminación por aguas residuales. (Tomado de la fuente)Mangroves respond to biotic and abiotic factors, such as variable salinity and anthropogenic stress (e.g., pollution from urban waste). Such factors may also affect populations of microorganisms and even the relative abundance of genes involved in biological responses. This research aimed to identify genes associated with resistance and biosynthesis of antimicrobial compounds in mangrove soils subjected to contrasting salinities. Total DNA was extracted from rhizospheric soil samples from three areas with different salinities in a wastewater contaminated mangrove at the Ranchería River's mouth (La Guajira, Colombia) for sequencing using Illumina HiSeq 2500. Functional and taxonomic diversity analysis revealed a domain of 51 families and 182 genera of actinobacteria. The most common biosynthesis pathways were for streptomycin and monobactamics. It was possible to associate 43 genes associated with the synthesis of antimicrobial compounds, and the abundance of 24 of them was significantly influenced by salinity. The increase in salt concentration influenced the metabolic pathways and the differential abundance of genes associated with the synthesis of antimicrobial compounds (e.g., rfbB / rffG, INO1 / ISYNA1, rfbA / rffH, sat / met3, asd, lysC, proA, aspB, fabG). In addition, out of 29 genes involved in intrinsic antibiotic resistance, 16 were significantly influenced by salinity (e.g. aacA, oleC4, oleC5, vgb, acrB / mexB / adeJ / smeE / mtrD / cmeB, bpeF, mexF). It is concluded that the mechanisms of tolerance and adaptability to the conditions of saline stress would favor the synthesis of antimicrobial compounds in mangroves subject to contamination by wastewater. 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FEMS Microbiology Letters, 188, 87–98.EspecializadaDiversidad funcional de microorganismos asociados al ciclaje de C, N y P en el manglar la Ranchería (La Guajira) mediante un acercamiento de metatranscriptómicaMinisterio de Ciencia, Tecnología e InnovaciónLICENSElicense.txtlicense.txttext/plain; charset=utf-83964https://repositorio.unal.edu.co/bitstream/unal/79917/1/license.txtcccfe52f796b7c63423298c2d3365fc6MD51ORIGINAL1017211887.2021.pdf1017211887.2021.pdfTesis de Maestría en Bosques y Conservación Ambientalapplication/pdf1607439https://repositorio.unal.edu.co/bitstream/unal/79917/3/1017211887.2021.pdf204b153513d38a082ac15b5954dee213MD53THUMBNAIL1017211887.2021.pdf.jpg1017211887.2021.pdf.jpgGenerated Thumbnailimage/jpeg5423https://repositorio.unal.edu.co/bitstream/unal/79917/4/1017211887.2021.pdf.jpg2b40802323299d8242aa6ddf6451621cMD54unal/79917oai:repositorio.unal.edu.co:unal/799172024-07-25 23:15:19.155Repositorio Institucional Universidad Nacional de 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GVyZWNob3MgZGUgYXV0b3IgcXVlIGNvbmxsZXZlIGxhIGRpc3RyaWJ1Y2nDs24gZGUgZXN0b3MgYXJjaGl2b3MgeSBtZXRhZGF0b3MuCkFsIGhhY2VyIGNsaWMgZW4gZWwgc2lndWllbnRlIGJvdMOzbiwgdXN0ZWQgaW5kaWNhIHF1ZSBlc3TDoSBkZSBhY3VlcmRvIGNvbiBlc3RvcyB0w6lybWlub3MuCgpVTklWRVJTSURBRCBOQUNJT05BTCBERSBDT0xPTUJJQSAtIMOabHRpbWEgbW9kaWZpY2FjacOzbiAyNy8yMC8yMDIwCg==

Caracterización metagenómica de genes asociados a la síntesis y resistencia de compuestos antimicrobianos en suelos de manglar.

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