Effect of the tryptone concentration on the calcium carbonate biomineralization mediated by Bacillus cereus

Five tryptone concentrations (0.2%, 0.4%, 0.6%, 0.8% and 1.0%) were evaluated on calcium carbonate biomineralization processes, using 0.5% of calcium acetate. A culture of Bacillus cereus, isolated from the gardens of The Universidad Pontificia Bolivariana (Medellin, Antioquia, Colombia) was used in all the assays. The experiments were monitored by pH measurements and mineralogical analyses of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Scanning electronic microscopy (SEM). Vaterite was the predominant polymorph in the precipitates after 6 days of the process. However, a considerable proportion of calcite appeared (above 30%) when the tryptone concentration was 0.4% or below. Additionally, the other experiments had a similar precipitate formation (between 3.2-3.6 g/L) except the assay using 0.2% of tryptone, which presented a low calcium carbonate production (1.79 g/L). A tryptone concentration below 0.4% would limit the bacterial metabolism and CO2 formation, necessary for calcium carbonate production.

• Autores: Caicedo-Pineda, Gerardo Andrés; Prada-Fonseca, María Consuelo; Casas-Botero, Ana Elisa; Martínez Tejada, Hader Vladimir
• Tipo de recurso: Article of journal
• Fecha de publicación: 2018
• Institución: Universidad Nacional de Colombia
• Repositorio: Universidad Nacional de Colombia
• Idioma: spa
• OAI Identifier: oai:repositorio.unal.edu.co:unal/68490
• Acceso en línea: https://repositorio.unal.edu.co/handle/unal/68490; http://bdigital.unal.edu.co/69523/
• Palabra clave: 62 Ingeniería y operaciones afines / Engineering; Bacillus cereus; biomineralization; calcium carbonate; tryptone; calcite; vaterite; Bacillus cereus; biomineralización; carbonato de calcio; triptona; calcita; vaterita
• Rights: openAccess
• License: Atribución-NoComercial 4.0 Internacional