Effect of the tryptone concentration on the calcium carbonate biomineralization mediated by Bacillus cereus

Five tryptone concentrations (0.2%, 0.4%, 0.6%, 0.8% and 1.0%) were evaluated on calcium carbonate biomineralization processes, using 0.5% of calcium acetate. A culture of Bacillus cereus, isolated from the gardens of The Universidad Pontificia Bolivariana (Medellin, Antioquia, Colombia) was used in...

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Autores:
Caicedo-Pineda, Gerardo Andrés
Prada-Fonseca, María Consuelo
Casas-Botero, Ana Elisa
Martínez Tejada, Hader Vladimir
Tipo de recurso:
Article of journal
Fecha de publicación:
2018
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/68490
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/68490
http://bdigital.unal.edu.co/69523/
Palabra clave:
62 Ingeniería y operaciones afines / Engineering
Bacillus cereus
biomineralization
calcium carbonate
tryptone
calcite
vaterite
Bacillus cereus
biomineralización
carbonato de calcio
triptona
calcita
vaterita
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
Description
Summary:Five tryptone concentrations (0.2%, 0.4%, 0.6%, 0.8% and 1.0%) were evaluated on calcium carbonate biomineralization processes, using 0.5% of calcium acetate. A culture of Bacillus cereus, isolated from the gardens of The Universidad Pontificia Bolivariana (Medellin, Antioquia, Colombia) was used in all the assays. The experiments were monitored by pH measurements and mineralogical analyses of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Scanning electronic microscopy (SEM). Vaterite was the predominant polymorph in the precipitates after 6 days of the process. However, a considerable proportion of calcite appeared (above 30%) when the tryptone concentration was 0.4% or below. Additionally, the other experiments had a similar precipitate formation (between 3.2-3.6 g/L) except the assay using 0.2% of tryptone, which presented a low calcium carbonate production (1.79 g/L). A tryptone concentration below 0.4% would limit the bacterial metabolism and CO2 formation, necessary for calcium carbonate production.