Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19

Ilustraciones a color, diagramas

Autores:
Hernández Bocanegra, Natalia Andrea
Tipo de recurso:
Fecha de publicación:
2024
Institución:
Universidad Nacional de Colombia
Repositorio:
Universidad Nacional de Colombia
Idioma:
spa
OAI Identifier:
oai:repositorio.unal.edu.co:unal/86453
Acceso en línea:
https://repositorio.unal.edu.co/handle/unal/86453
https://repositorio.unal.edu.co/
Palabra clave:
570 - Biología::576 - Genética y evolución
Receptor ACE2 humano
región Spike
variante
linaje viral
Human ACE2 receptor
Spike región
variant
viral lineage
ACE2
Enzima convertidora de angiotensina
Spike
Covid-19
SARS-CoV-2
Rights
openAccess
License
Atribución-NoComercial 4.0 Internacional
id UNACIONAL2_4c89bddd96bcfd7932395dbdb7cc01ce
oai_identifier_str oai:repositorio.unal.edu.co:unal/86453
network_acronym_str UNACIONAL2
network_name_str Universidad Nacional de Colombia
repository_id_str
dc.title.spa.fl_str_mv Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19
dc.title.translated.eng.fl_str_mv Analysis of genetic variability in seven exons of the human ACE2 gene and its relationship with the SARS-CoV-2 lineage in a group of individuals diagnosed with COVID-19
title Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19
spellingShingle Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19
570 - Biología::576 - Genética y evolución
Receptor ACE2 humano
región Spike
variante
linaje viral
Human ACE2 receptor
Spike región
variant
viral lineage
ACE2
Enzima convertidora de angiotensina
Spike
Covid-19
SARS-CoV-2
title_short Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19
title_full Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19
title_fullStr Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19
title_full_unstemmed Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19
title_sort Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19
dc.creator.fl_str_mv Hernández Bocanegra, Natalia Andrea
dc.contributor.advisor.none.fl_str_mv Pinzón Velasco, Andrés Mauricio
dc.contributor.author.none.fl_str_mv Hernández Bocanegra, Natalia Andrea
dc.contributor.researchgroup.spa.fl_str_mv Bioinformática
dc.contributor.orcid.spa.fl_str_mv https://orcid.org/0009-0001-4161-6069
dc.subject.ddc.spa.fl_str_mv 570 - Biología::576 - Genética y evolución
topic 570 - Biología::576 - Genética y evolución
Receptor ACE2 humano
región Spike
variante
linaje viral
Human ACE2 receptor
Spike región
variant
viral lineage
ACE2
Enzima convertidora de angiotensina
Spike
Covid-19
SARS-CoV-2
dc.subject.proposal.spa.fl_str_mv Receptor ACE2 humano
región Spike
variante
linaje viral
dc.subject.proposal.eng.fl_str_mv Human ACE2 receptor
Spike región
variant
viral lineage
dc.subject.wikidata.none.fl_str_mv ACE2
Enzima convertidora de angiotensina
Spike
dc.subject.bne.none.fl_str_mv Covid-19
SARS-CoV-2
description Ilustraciones a color, diagramas
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-07-16T15:25:52Z
dc.date.available.none.fl_str_mv 2024-07-16T15:25:52Z
dc.date.issued.none.fl_str_mv 2024
dc.type.spa.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.spa.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.spa.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.spa.fl_str_mv Text
dc.type.redcol.spa.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unal.edu.co/handle/unal/86453
dc.identifier.instname.spa.fl_str_mv Universidad Nacional de Colombia
dc.identifier.reponame.spa.fl_str_mv Repositorio Institucional Universidad Nacional de Colombia
dc.identifier.repourl.spa.fl_str_mv https://repositorio.unal.edu.co/
url https://repositorio.unal.edu.co/handle/unal/86453
https://repositorio.unal.edu.co/
identifier_str_mv Universidad Nacional de Colombia
Repositorio Institucional Universidad Nacional de Colombia
dc.language.iso.spa.fl_str_mv spa
language spa
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dc.coverage.temporal.none.fl_str_mv 2020-2021
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dc.publisher.branch.spa.fl_str_mv Universidad Nacional de Colombia - Sede Bogotá
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spelling Atribución-NoComercial 4.0 Internacionalinfo:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Pinzón Velasco, Andrés Mauricio366c2eddf6aa24434ee55e57da235448Hernández Bocanegra, Natalia Andreaca6fc7d8b6afea7694b18a21e26548d4Bioinformáticahttps://orcid.org/0009-0001-4161-60692020-2021Bogotá, Colombia2024-07-16T15:25:52Z2024-07-16T15:25:52Z2024https://repositorio.unal.edu.co/handle/unal/86453Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/Ilustraciones a color, diagramasThe interaction between the Spike protein of SARS-CoV-2 and the human ACE2 receptor plays a pivotal role in the development of COVID-19. This study aimed to assess the genetic variability of the viral lineage and seven exons of the human ACE2 gene, along with analyzing clinical backgrounds and sociodemographic conditions related to susceptibility and disease severity. Using sequencing technology, samples from nasopharyngeal swabs were examined for the viral genome, and blood samples from 50 individuals diagnosed with SARS-CoV-2 infection or viral pneumonia were sequenced. Although no statistically significant associations were found between viral lineages and disease severity, it was observed that the B.1.621 lineage predominated in mild cases, while the B.1.420 lineage was present in moderate cases, and the P.1 lineage was found in both moderate and severe individuals. Analysis of exonic sequences of the ACE2 gene revealed a homozygous variant in exon 17, involving a synonymous substitution at position 133, resulting in the encoding of valine. No significant associations were found between demographic data, clinical history, and disease severity. These results indicate genetic variability in the studied regions of the ACE2 gene, underscoring the importance of future large-scale studies with representative samples to comprehend the virus's pathophysiology and its relationship with the developed phenotype.La interacción entre la proteína Spike del SARS-CoV-2 y el receptor ACE2 humano es crucial en el desarrollo de la enfermedad COVID-19. Este estudio buscó evaluar la variabilidad genética del linaje viral y de siete exones del gen ACE2 humano, así como analizar antecedentes clínicos y condiciones sociodemográficas relacionadas con la susceptibilidad y severidad de la enfermedad. Utilizando tecnología de secuenciación, se examinaron muestras de hisopado nasofaríngeo para el genoma viral y muestras sanguíneas de 50 individuos diagnosticados con infección o neumonía viral por SARS-CoV-2. Estos individuos fueron ingresados a la Clínica Universitaria Colombia, ubicada en la ciudad de Bogotá, desde octubre del año 2020 a junio del año 2021, y se clasificaron en tres grupos de severidad según los síntomas, el diagnóstico, la insuficiencia respiratoria y la necesidad de ventilación como: leve, moderado y severo. A pesar de no encontrar asociaciones estadísticamente significativas entre los linajes virales y la gravedad de la enfermedad, se observó que el linaje B.1.621 predomina en casos leves, mientras que el linaje B.1.420 está presente solo en casos moderados y el linaje P.1 se encontró en individuos moderados y severos. El análisis de las secuencias de los siete exones del gen ACE2 reveló una única variante homocigota en el exón 17; que corresponde a la sustitución de una guanina (G) por una adenina (A), causando un cambio sinónimo en la posición 748 de la proteína ACE2 que resulta en la codificación de una valina. No se encontraron correlaciones significativamente estadísticas entre datos demográficos, antecedentes clínicos y gravedad de la enfermedad. Se requieren estudios futuros con muestras representativas para comprender la fisiopatología del virus y su relación con el fenotipo desarrollado. (Texto tomado de la fuente)MaestríaBiología molecular de agentes infecciososviii, 71 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - MicrobiologíaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá570 - Biología::576 - Genética y evoluciónReceptor ACE2 humanoregión Spikevariantelinaje viralHuman ACE2 receptorSpike regiónvariantviral lineageACE2Enzima convertidora de angiotensinaSpikeCovid-19SARS-CoV-2Análisis de la variabilidad genética en siete exones del gen ACE2 humano y su relación con el linaje de SARS-CoV-2 en un grupo de individuos diagnosticados con COVID-19Analysis of genetic variability in seven exons of the human ACE2 gene and its relationship with the SARS-CoV-2 lineage in a group of individuals diagnosed with COVID-19Trabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAbdullaev, A., Abdurakhimov, A., Mirakbarova, Z., Ibragimova, S., Tsoy, V., Nuriddinov, S., Dalimova, D., Turdikulova, S., & Abdurakhmonov, I. 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Journal of Virology, 85(9), 4122–4134. https://doi.org/10.1128/JVI.02232-10BibliotecariosEstudiantesInvestigadoresPersonal de apoyo escolarPúblico generalLICENSElicense.txtlicense.txttext/plain; charset=utf-85879https://repositorio.unal.edu.co/bitstream/unal/86453/1/license.txteb34b1cf90b7e1103fc9dfd26be24b4aMD51ORIGINAL1030666404.2024.pdf1030666404.2024.pdfapplication/pdf1416032https://repositorio.unal.edu.co/bitstream/unal/86453/2/1030666404.2024.pdf194bcde4506763c6ad73fd6f45efd53dMD52THUMBNAIL1030666404.2024.pdf.jpg1030666404.2024.pdf.jpgGenerated Thumbnailimage/jpeg5594https://repositorio.unal.edu.co/bitstream/unal/86453/3/1030666404.2024.pdf.jpg4df2a62f88cd2b5ade038a7f5a3f48a6MD53unal/86453oai:repositorio.unal.edu.co:unal/864532024-07-16 23:05:09.03Repositorio Institucional Universidad Nacional de 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