Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular
ilustraciones, diagramas
- Autores:
-
Urrego Orrego, Karen Yurany
- Tipo de recurso:
- Fecha de publicación:
- 2024
- Institución:
- Universidad Nacional de Colombia
- Repositorio:
- Universidad Nacional de Colombia
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unal.edu.co:unal/86328
- Palabra clave:
- 610 - Medicina y salud::612 - Fisiología humana
570 - Biología::572 - Bioquímica
Acondicionamiento Pretrasplante
Biotecnología
Congelación
Cordón Umbilical
Transplantation Conditioning
Biotechnology
Freezing
Umbilical Cord
Sangre de cordón umbilical
Trasplante de progenitores hematopoyéticos
Criopreservación
Dilución
Lavado
Apoptosis
Necrosis
Umbilical cord blood
Hematopoietic stem cell transplantation
Cryopreservation
Dilution
Washing
Apoptosis
Necrosis
- Rights
- openAccess
- License
- Reconocimiento 4.0 Internacional
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Universidad Nacional de Colombia |
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| dc.title.spa.fl_str_mv |
Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular |
| dc.title.translated.eng.fl_str_mv |
Pretransplantation procedure optimization of umbilical cord blood units thawing: prevention of cell death |
| title |
Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular |
| spellingShingle |
Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular 610 - Medicina y salud::612 - Fisiología humana 570 - Biología::572 - Bioquímica Acondicionamiento Pretrasplante Biotecnología Congelación Cordón Umbilical Transplantation Conditioning Biotechnology Freezing Umbilical Cord Sangre de cordón umbilical Trasplante de progenitores hematopoyéticos Criopreservación Dilución Lavado Apoptosis Necrosis Umbilical cord blood Hematopoietic stem cell transplantation Cryopreservation Dilution Washing Apoptosis Necrosis |
| title_short |
Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular |
| title_full |
Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular |
| title_fullStr |
Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular |
| title_full_unstemmed |
Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular |
| title_sort |
Optimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celular |
| dc.creator.fl_str_mv |
Urrego Orrego, Karen Yurany |
| dc.contributor.advisor.none.fl_str_mv |
Perdomo Arciniegas, Ana María Fontanilla Duque, Martha Raquel |
| dc.contributor.author.none.fl_str_mv |
Urrego Orrego, Karen Yurany |
| dc.contributor.researchgroup.spa.fl_str_mv |
Grupo de Investigación en Medicina transfusional, tisular y celular (GYMTIC) |
| dc.contributor.orcid.spa.fl_str_mv |
Urrego Orrego, Karen Yurany [000000023762826X] |
| dc.subject.ddc.spa.fl_str_mv |
610 - Medicina y salud::612 - Fisiología humana 570 - Biología::572 - Bioquímica |
| topic |
610 - Medicina y salud::612 - Fisiología humana 570 - Biología::572 - Bioquímica Acondicionamiento Pretrasplante Biotecnología Congelación Cordón Umbilical Transplantation Conditioning Biotechnology Freezing Umbilical Cord Sangre de cordón umbilical Trasplante de progenitores hematopoyéticos Criopreservación Dilución Lavado Apoptosis Necrosis Umbilical cord blood Hematopoietic stem cell transplantation Cryopreservation Dilution Washing Apoptosis Necrosis |
| dc.subject.decs.spa.fl_str_mv |
Acondicionamiento Pretrasplante Biotecnología Congelación Cordón Umbilical |
| dc.subject.decs.eng.fl_str_mv |
Transplantation Conditioning Biotechnology Freezing Umbilical Cord |
| dc.subject.proposal.spa.fl_str_mv |
Sangre de cordón umbilical Trasplante de progenitores hematopoyéticos Criopreservación Dilución Lavado Apoptosis Necrosis |
| dc.subject.proposal.eng.fl_str_mv |
Umbilical cord blood Hematopoietic stem cell transplantation Cryopreservation Dilution Washing Apoptosis Necrosis |
| description |
ilustraciones, diagramas |
| publishDate |
2024 |
| dc.date.accessioned.none.fl_str_mv |
2024-06-28T14:37:21Z |
| dc.date.available.none.fl_str_mv |
2024-06-28T14:37:21Z |
| dc.date.issued.none.fl_str_mv |
2024 |
| dc.type.spa.fl_str_mv |
Trabajo de grado - Maestría |
| dc.type.driver.spa.fl_str_mv |
info:eu-repo/semantics/masterThesis |
| dc.type.version.spa.fl_str_mv |
info:eu-repo/semantics/acceptedVersion |
| dc.type.content.spa.fl_str_mv |
Text |
| dc.type.redcol.spa.fl_str_mv |
http://purl.org/redcol/resource_type/TM |
| status_str |
acceptedVersion |
| dc.identifier.uri.none.fl_str_mv |
https://repositorio.unal.edu.co/handle/unal/86328 |
| dc.identifier.instname.spa.fl_str_mv |
Universidad Nacional de Colombia |
| dc.identifier.reponame.spa.fl_str_mv |
Repositorio Institucional Universidad Nacional de Colombia |
| dc.identifier.repourl.spa.fl_str_mv |
https://repositorio.unal.edu.co/ |
| url |
https://repositorio.unal.edu.co/handle/unal/86328 https://repositorio.unal.edu.co/ |
| identifier_str_mv |
Universidad Nacional de Colombia Repositorio Institucional Universidad Nacional de Colombia |
| dc.language.iso.spa.fl_str_mv |
spa |
| language |
spa |
| dc.relation.references.spa.fl_str_mv |
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Umbilical cord blood transplantation: the first 25 years and beyond. Blood, 122(4), 491–498. https://doi.org/10.1182/BLOOD-2013-02-453175 Ballen, K. K., Verter, F., & Kurtzberg, J. (2015). Umbilical cord blood donation: Public or private? In Bone Marrow Transplantation (Vol. 50, Issue 10, pp. 1271–1278). Nature Publishing Group. https://doi.org/10.1038/bmt.2015.124 Baust, J. G., Snyder, K. K., Van Buskirk, R., & Baust, J. M. (2017). Integrating Molecular Control to Improve Cryopreservation Outcome. Https://Home.Liebertpub.Com/Bio, 15(2), 134–141. https://doi.org/10.1089/BIO.2016.0119 Berz, D., McCormack, E. M., Winer, E. S., Colvin, G. A., & Quesenberry, P. J. (2007). Cryopreservation of Hematopoietic Stem Cells. American Journal of Hematology, 82(6), 463. https://doi.org/10.1002/AJH.20707 Bhattacharya, S. (2016). A Review on Cryoprotectant and its Modern Implication in Cryonics | Semantic Scholar. https://www.semanticscholar.org/paper/A-Review-on-Cryoprotectant-and-its-Modern-in-Bhattacharya/6bf59e0acdb26b2a60c9bd549ef497f4b5e0762e Bissoyi, A., Nayak, B., Pramanik, K., & Sarangi, S. K. (2014). Targeting cryopreservation-induced cell death: a review. Biopreservation and Biobanking, 12(1), 23–34. https://doi.org/10.1089/BIO.2013.0032 Bojic, S., Murray, A., Bentley, B. L., Spindler, R., Pawlik, P., Cordeiro, J. L., Bauer, R., & de Magalhães, J. P. (2021). Winter is coming: the future of cryopreservation. BMC Biology 2021 19:1, 19(1), 1–20. https://doi.org/10.1186/S12915-021-00976-8 Broxmeyer, H. E., Douglas, G. W., Hangoc, G., Cooper, S., Bard, J., English, D., Arny, M., Thomas, L., & Boyse, E. A. (1989). Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. Proceedings of the National Academy of Sciences of the United States of America, 86(10), 3828. https://doi.org/10.1073/PNAS.86.10.3828 Builes, N., Niño-Serna, L., & Combariza, J. F. (2023). Side effects after use of bedside thaw method in an umbilical cord blood stem cells for allogeneic transplantation in a children cohort: A single-center experience. Hematology/Oncology and Stem Cell Therapy, 17(1). https://doi.org/10.56875/2589-0646.1110 Campos De Carvalho, E., Carolina, A., & Vieira Curcioli, J. (2010). Infusión de células madre hematopoyéticas: tipos, características, reacciones adversas y de transfusión y sus implicaciones para la enfermería1. Scielo.Br. Retrieved January 24, 2024, from https://www.scielo.br/j/rlae/a/zrStPfTGbcFPL9Y8SFSYvGf/?format=pdf&lang=es Caocci, G., Greco, M., & La Nasa, G. (2017). Bone Marrow Homing and Engraftment Defects of Human Hematopoietic Stem and Progenitor Cells. Mediterranean Journal of Hematology and Infectious Diseases, 9(1), 2017032. https://doi.org/10.4084/MJHID.2017.032 Cava, C. (2015). Comparación de dos soluciones de Lavado para el Trasplante de Progenitores Hematopoyéticos sin Dimetil Sulfóxido. PDF Descarga libre. https://digitum.um.es/digitum/bitstream/10201/47027/1/Catalina%20Cava%20Tesis%20Doctoral.pdf Chang, T., & Zhao, G. (2021). Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges. Advanced Science, 8(6), 2002425. https://doi.org/10.1002/ADVS.202002425 Cheng, H., Zheng, Z., & Cheng, T. (2020). New paradigms on hematopoietic stem cell differentiation. Protein and Cell, 11(1), 34–44. https://doi.org/10.1007/S13238-019-0633-0/FIGURES/4 Chow, R. Y. K., Li, Q., Chow, C., Guo, V., Dang, T., Rao, A., Zeng, T., Chow, D. T.-L., Wang, B., & Chow, M. (2017). Cord Blood Stem Cell Processing, Banking and Thawing. 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Trasplante de células progenitoras hematopoyéticas: tipos, fuentes e indicaciones. Rev Cubana de Hematología, Inmunología y Hemoterapia. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-02892004000200002 Jang, T. H., Park, S. C., Yang, J. H., Kim, J. Y., Seok, J. H., Park, U. S., Choi, C. W., Lee, S. R., & Han, J. (2017). Cryopreservation and its clinical applications. Integrative Medicine Research, 6(1), 12–18. https://doi.org/10.1016/J.IMR.2016.12.001 Jara-Segura, E., Jensen-Gamboa, E., & Clínico Jensen, L. (2018). Recuento de células CD34+ por citometría de flujo Count of CD34 + cells by flow cytometry. Rev. Colegio de Microb. Quim. Clin. de Costa Rica, 24(2), 2215–3713. Johnson, D., Hashaikeh, R., & Hilal, N. (2021). Basic principles of osmosis and osmotic pressure. Osmosis Engineering, 1–15. https://doi.org/10.1016/B978-0-12-821016-1.00011-5 Karam Khaddour; Caroline K. Hana; Prerna Mewawalla. (2022). Hematopoietic Stem Cell Transplantation. 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Reconocimiento 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Perdomo Arciniegas, Ana María5d532cf1dbb19f57c31006442e91ee7eFontanilla Duque, Martha Raquelf299f62941972508549340696d2c4908Urrego Orrego, Karen Yurany71d848b8bf1ea05194a4fa0194adc6d9Grupo de Investigación en Medicina transfusional, tisular y celular (GYMTIC)Urrego Orrego, Karen Yurany [000000023762826X]2024-06-28T14:37:21Z2024-06-28T14:37:21Z2024https://repositorio.unal.edu.co/handle/unal/86328Universidad Nacional de ColombiaRepositorio Institucional Universidad Nacional de Colombiahttps://repositorio.unal.edu.co/ilustraciones, diagramasLa sangre de cordón umbilical (SCU) es una fuente de progenitores hematopoyéticos (PH) usados como terapia en diversas patologías, principalmente de tipo hematológico. En Colombia, el grupo de investigación en medicina transfusional, tisular y celular (GIMTTYC) del Instituto Distrital de Ciencia, Biotecnología e Innovación en Salud (IDCBIS), desarrolló un programa clínico de captación de donantes y de banqueo de células de SCU, así como servicios de búsqueda de compatibilidad, reserva y distribución de unidades, bajo estándares internacionales de terapia celular. Los procedimientos de congelación y descongelación de SCU se validan en cada banco con el fin de mantener viables las células que se usarán en el trasplante. La variación de las condiciones en estos procedimientos impacta la recuperación y viabilidad celular, que puede afectar la potencia terapéutica de los trasplantes. Con el fin de corroborar la funcionalidad de las células, antes y después de la descongelación de las unidades de SCU, se realizan pruebas de viabilidad celular por citometría de flujo y clonogenicidad. Por lo tanto, establecer las condiciones para mantener el número y la viabilidad celular después de la criopreservación, antes del trasplante es clave para garantizar la calidad de unidades en esta etapa. Para descongelar las unidades de SCU, estas se diluyen o lavan para disminuir el efecto citotóxico del crioprotector. Sin embargo, el GIMTTYC todavía requiere estandarizar el procedimiento de descongelación. En este trabajo se validó la implementación in situ de un protocolo pretrasplante de descongelación de unidades de SCU con la perspectiva de establecer herramientas que mejoren la recuperación y viabilidad celular pretrasplante. (Texto tomado de la fuente)Umbilical cord blood (UCB) is a source of hematopoietic progenitors (HP), which are used as therapy in various pathologies, mainly hematological. In Colombia, the transfusion, tissue and cellular medicine research group (GIMTTYC) of the Instituto Distrital de Ciencia, Biotecnología e Innovación en Salud (IDCBIS), developed a clinical program for donor recruitment and banking of SCU cells, as well as services of compatibility search, reservation and distribution of units, under international standards of cellular therapy. SCU freezing and thawing procedures are validated in each bank in order to maintain the viability of the cells to be used in transplantation. Varying conditions in these procedures impact cell recovery and viability, which can affect the therapeutic potency of transplants. In order to corroborate that the cells will be functional, before and after thawing of the SCU units, cell viability tests are performed by flow cytometry and clonogenicity. Therefore, establishing the conditions to maintain cell number and viability after cryopreservation prior to transplantation is key to ensure the quality of units at this stage. To thaw SCU units prior to transplantation, they are diluted or washed to decrease the cytotoxic effect of the cryoprotectant. However, in the GIMTTYC this process still requires standardization. This work validated the in situ implementation of a pretransplantation protocol for thawing SCU units with the perspective of establishing tools to improve pretransplantation cell recovery and viability.MaestríaMagíster en Ciencias - BioquímicaInvestigación en fisiología y patología de la hematopoyesis para el desarrollo y la innovación clínicaxviii, 90 páginasapplication/pdfspaUniversidad Nacional de ColombiaBogotá - Ciencias - Maestría en Ciencias - BioquímicaFacultad de CienciasBogotá, ColombiaUniversidad Nacional de Colombia - Sede Bogotá610 - Medicina y salud::612 - Fisiología humana570 - Biología::572 - BioquímicaAcondicionamiento PretrasplanteBiotecnologíaCongelaciónCordón UmbilicalTransplantation ConditioningBiotechnologyFreezingUmbilical CordSangre de cordón umbilicalTrasplante de progenitores hematopoyéticosCriopreservaciónDiluciónLavadoApoptosisNecrosisUmbilical cord bloodHematopoietic stem cell transplantationCryopreservationDilutionWashingApoptosisNecrosisOptimización del procedimiento pretrasplante de descongelación de unidades de sangre de cordón umbilical: prevención en muerte celularPretransplantation procedure optimization of umbilical cord blood units thawing: prevention of cell deathTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAkel, S., Regan, D., Wall, D., Petz, L., & McCullough, J. 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