Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina

Objective To assess a LED-fluorescence microscopy (LED-FM) capacitation program for the training of laboratory technicians without previous experience in FM.Methods We evaluated a teaching program that consists of a three-day course followed by an “in situ” two-month phase in which technicians acqui...

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Autores:: Imaz, Maria Susana
Allassia, Sonia
Aranibar, Mónica
Gunia, Alba Marisa
Poggi, Susana
Togneri, Ana María
Wolff, Lidia

Tipo de recurso:: Article of journal

Fecha de publicación:: 2018

Institución:: Universidad Nacional de Colombia

Repositorio:: Universidad Nacional de Colombia

Idioma:: spa

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dc.title.spa.fl_str_mv	Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina
title	Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina
spellingShingle	Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina 36 Problemas y servicios sociales, asociaciones / Social problems and social services 61 Ciencias médicas; Medicina / Medicine and health Tuberculosis teaching fluorescence Tuberculosis capacitación fluorescencia
title_short	Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina
title_full	Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina
title_fullStr	Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina
title_full_unstemmed	Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina
title_sort	Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina
dc.creator.fl_str_mv	Imaz, Maria Susana Allassia, Sonia Aranibar, Mónica Gunia, Alba Marisa Poggi, Susana Togneri, Ana María Wolff, Lidia
dc.contributor.author.spa.fl_str_mv	Imaz, Maria Susana Allassia, Sonia Aranibar, Mónica Gunia, Alba Marisa Poggi, Susana Togneri, Ana María Wolff, Lidia
dc.subject.ddc.spa.fl_str_mv	36 Problemas y servicios sociales, asociaciones / Social problems and social services 61 Ciencias médicas; Medicina / Medicine and health
topic	36 Problemas y servicios sociales, asociaciones / Social problems and social services 61 Ciencias médicas; Medicina / Medicine and health Tuberculosis teaching fluorescence Tuberculosis capacitación fluorescencia
dc.subject.proposal.spa.fl_str_mv	Tuberculosis teaching fluorescence Tuberculosis capacitación fluorescencia
description	Objective To assess a LED-fluorescence microscopy (LED-FM) capacitation program for the training of laboratory technicians without previous experience in FM.Methods We evaluated a teaching program that consists of a three-day course followed by an “in situ” two-month phase in which technicians acquired skills without the help of a FM expert; in order to gain confidence to recognize auramine-stained bacillus, during this phase, technicians examined duplicate slides stained by Ziehl Neelsen (ZN) and FM in a unblinded way. Technicians with acceptable performance, continued with a blinded-training period. Testing panels and rechecking process were used to evaluate proficiency after different length of experience.Results Post-course panel results showed that 70% of trainees made Low False Positive errors (LFPs). Analysis of two other panels showed that LFPs significantly decreased (Chi-squared test, p0.05) as the “in situ” training phase progressed. Processing at least three slides/day was associated with acceptable performance. During the blinded-training period, results of the rechecking process showed that sensitivity (96.8%) and specificity (99.8%) levels were satisfactory.Conclusion Moderate training (a three-day course) is not enough to make technicians proficient in LED-FM; however,great ability can be reached after a short “in situ” training phase even without the presence of experienced staff available in field to review doubtful results. Training was more effective in services with a minimum workload of 750 slides/year.
publishDate	2018
dc.date.issued.spa.fl_str_mv	2018-01-01
dc.date.accessioned.spa.fl_str_mv	2019-07-03T00:20:36Z
dc.date.available.spa.fl_str_mv	2019-07-03T00:20:36Z
dc.type.spa.fl_str_mv	Artículo de revista
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dc.identifier.issn.spa.fl_str_mv	ISSN: 2539-3596
dc.identifier.uri.none.fl_str_mv	https://repositorio.unal.edu.co/handle/unal/65430
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identifier_str_mv	ISSN: 2539-3596
url	https://repositorio.unal.edu.co/handle/unal/65430 http://bdigital.unal.edu.co/66453/
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language	spa
dc.relation.spa.fl_str_mv	https://revistas.unal.edu.co/index.php/revsaludpublica/article/view/61402
dc.relation.ispartof.spa.fl_str_mv	Universidad Nacional de Colombia Revistas electrónicas UN Revista de Salud Pública Revista de Salud Pública
dc.relation.references.spa.fl_str_mv	Imaz, Maria Susana and Allassia, Sonia and Aranibar, Mónica and Gunia, Alba Marisa and Poggi, Susana and Togneri, Ana María and Wolff, Lidia (2018) Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina. Revista de Salud Pública, 20 (1). pp. 110-116. ISSN 2539-3596
dc.rights.spa.fl_str_mv	Derechos reservados - Universidad Nacional de Colombia
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dc.rights.license.spa.fl_str_mv	Atribución-NoComercial 4.0 Internacional
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rights_invalid_str_mv	Atribución-NoComercial 4.0 Internacional Derechos reservados - Universidad Nacional de Colombia http://creativecommons.org/licenses/by-nc/4.0/ http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
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dc.publisher.spa.fl_str_mv	Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina - Instituto de Salud Pública
institution	Universidad Nacional de Colombia
bitstream.url.fl_str_mv	https://repositorio.unal.edu.co/bitstream/unal/65430/1/61402-388887-1-PB.pdf https://repositorio.unal.edu.co/bitstream/unal/65430/2/61402-388887-1-PB.pdf.jpg
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spelling	Atribución-NoComercial 4.0 InternacionalDerechos reservados - Universidad Nacional de Colombiahttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Imaz, Maria Susanab95d7f30-a0b5-4dee-a6ec-e5e243a170b7300Allassia, Sonia3ce3390b-ae4c-443a-b9b5-b45174a7e3b6300Aranibar, Mónicad8f1d1a6-0005-4123-b408-fd1789b3cf00300Gunia, Alba Marisa3b539476-74dd-44b7-8f67-6c857cf7776b300Poggi, Susanac7b313ef-b58e-4fdc-ad1a-97650d74362b300Togneri, Ana María8dc0f1b0-4d02-4028-ae5c-e41005738de4300Wolff, Lidia34b23b78-cf7c-4507-aab7-6fbb5043e1f13002019-07-03T00:20:36Z2019-07-03T00:20:36Z2018-01-01ISSN: 2539-3596https://repositorio.unal.edu.co/handle/unal/65430http://bdigital.unal.edu.co/66453/Objective To assess a LED-fluorescence microscopy (LED-FM) capacitation program for the training of laboratory technicians without previous experience in FM.Methods We evaluated a teaching program that consists of a three-day course followed by an “in situ” two-month phase in which technicians acquired skills without the help of a FM expert; in order to gain confidence to recognize auramine-stained bacillus, during this phase, technicians examined duplicate slides stained by Ziehl Neelsen (ZN) and FM in a unblinded way. Technicians with acceptable performance, continued with a blinded-training period. Testing panels and rechecking process were used to evaluate proficiency after different length of experience.Results Post-course panel results showed that 70% of trainees made Low False Positive errors (LFPs). Analysis of two other panels showed that LFPs significantly decreased (Chi-squared test, p0.05) as the “in situ” training phase progressed. Processing at least three slides/day was associated with acceptable performance. During the blinded-training period, results of the rechecking process showed that sensitivity (96.8%) and specificity (99.8%) levels were satisfactory.Conclusion Moderate training (a three-day course) is not enough to make technicians proficient in LED-FM; however,great ability can be reached after a short “in situ” training phase even without the presence of experienced staff available in field to review doubtful results. Training was more effective in services with a minimum workload of 750 slides/year.Objetivo Evaluar un programa de capacitación en microscopía de fluorescencia LED (MF-LED) para el entrenamiento de técnicos de laboratorio sin experiencia en MF.Métodos Se evaluó un programa de capacitación que consiste en un curso de tres días seguido de dos meses de entrenamiento «in situ», en donde los técnicos adquirieron habilidades sin presencia de un experto en la práctica diaria; para alcanzar confianza en el reconocimiento del bacilo, los técnicos, durante estos meses, examinaron en forma «no cegada» extendidos duplicados teñidos por Ziehl Neelsen (ZN) y MF. Aquellos laboratoristas que lograron rendimiento aceptable continuaron su entrenamiento «a ciegas». Su desempeño fue evaluado en distintos períodos del entrenamiento mediante paneles de láminas y relectura de extendidos.Resultados Los resultados de un panel posterior al curso mostraron que 70% de los participantes cometieron errores falsos positivos bajos (FPB). Dos paneles posteriores evidenciaron que los FPB disminuían significativamente (prueba de Chi cuadrado, p0.05) a medida que el entrenamiento avanzaba. El procesamiento de al menos tres extendidos/día se asoció con desempeño aceptable. Durante el período a ciegas, la relectura de láminas evidenció que la sensibilidad (96,8%) y especificidad (99,8%) fueron satisfactorias.Conclusiones Una capacitación moderada (curso de tres días) no es suficiente para adquirir competencia en MF-LED;sin embargo, se puede alcanzar habilidad después de una corta capacitación «in situ», incluso si no hay personal conexperiencia disponible en el servicio para revisar los resultados dudosos diariamente. El entrenamiento fue más efectivoen servicios con carga de trabajo mínima de 750 extendidos/año.application/pdfspaUniversidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina - Instituto de Salud Públicahttps://revistas.unal.edu.co/index.php/revsaludpublica/article/view/61402Universidad Nacional de Colombia Revistas electrónicas UN Revista de Salud PúblicaRevista de Salud PúblicaImaz, Maria Susana and Allassia, Sonia and Aranibar, Mónica and Gunia, Alba Marisa and Poggi, Susana and Togneri, Ana María and Wolff, Lidia (2018) Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina. Revista de Salud Pública, 20 (1). pp. 110-116. ISSN 2539-359636 Problemas y servicios sociales, asociaciones / Social problems and social services61 Ciencias médicas; Medicina / Medicine and healthTuberculosisteachingfluorescenceTuberculosiscapacitaciónfluorescenciaTraining tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in ArgentinaArtículo de revistainfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1http://purl.org/coar/version/c_970fb48d4fbd8a85Texthttp://purl.org/redcol/resource_type/ARTORIGINAL61402-388887-1-PB.pdfapplication/pdf320538https://repositorio.unal.edu.co/bitstream/unal/65430/1/61402-388887-1-PB.pdf685030a646ab37ecdd273d5879b0c00eMD51THUMBNAIL61402-388887-1-PB.pdf.jpg61402-388887-1-PB.pdf.jpgGenerated Thumbnailimage/jpeg8132https://repositorio.unal.edu.co/bitstream/unal/65430/2/61402-388887-1-PB.pdf.jpg9e8f8ff0658c129dfd9c14d958ee2feeMD52unal/65430oai:repositorio.unal.edu.co:unal/654302023-05-19 23:47:07.685Repositorio Institucional Universidad Nacional de Colombiarepositorio_nal@unal.edu.co

Training tuberculosis laboratory workers in LED-fluorescence microscopy: experience learned in Argentina

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